Friday, August 09, 2024


Smooth sailing for eggplant: breakthrough in understanding prickle formation



 News Release 

Nanjing Agricultural University The Academy of Science

Morphological characterization of prickle in eggplant. 

image: 

Morphological characterization of prickle in eggplant. ah Phenotype comparison between two parental lines in different tissues: stems (ab), leaves (cd), flowers (ef), and fruits (gh) from the prickly eggplant ‘PI 381159’ and the prickleless eggplant ‘XQ23’, respectively. Black bars represent scales of 1 cm. i Scanning electron micrographs of longitudinally sliced prickles. j Magnified representation of the section highlighted by the white square in graph, illustrating the junction between the prickle and the stem epidermis. Black bars represent scales of 1 mm (ij).

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Credit: Horticulture Research




Scientists have discovered the gene responsible for prickles in eggplants, a trait that complicates farming. Using advanced genetic techniques, they identified the Prickly Eggplant (PE) gene on chromosome 6 and pinpointed SmLOG1 as the key factor. CRISPR-Cas9 gene editing confirmed that disabling SmLOG1 eliminates prickles, paving the way for prickle-free eggplant varieties. This breakthrough not only sheds light on prickle development but also promises to streamline eggplant cultivation and harvesting, benefiting the agricultural industry.

Eggplants, a staple crop globally, present significant challenges in cultivation and harvesting due to their prickles. These prickles, which serve as a natural defense mechanism, complicate handling and reduce efficiency in agricultural practices. Addressing these issues is crucial for improving farming outcomes. Consequently, researchers are focused on understanding the genetic basis of prickle formation to develop prickle-free eggplant varieties. Based on these challenges, there is a pressing need to conduct in-depth research and identify solutions for more efficient eggplant cultivation.

Researchers from Jiangsu Normal University, in collaboration with the Chinese Academy of Agricultural Sciences, have made significant strides in this area. Their findings (DOI: 10.1093/hr/uhae134), published in the prestigious journal Horticulture Research on May 10, 2024, focus on the identification of a key gene responsible for the prickly phenotype in eggplants.

The research team employed bulk segregant RNA-sequencing (BSR-seq) and linkage analysis to identify the Prickly Eggplant (PE) gene on chromosome 6, which is crucial for prickle formation in eggplants. They discovered that the SmLOG1 gene, encoding a cytokinin biosynthetic enzyme, plays a pivotal role in this process. Through CRISPR-Cas9 gene editing, they knocked out SmLOG1 in prickly eggplants, resulting in plants completely devoid of prickles. This confirmed the gene's essential role in prickle development. Furthermore, the study identified a specific SNP in the SmLOG1 promoter region associated with prickle variation, which can be used as a molecular marker for breeding. This groundbreaking research not only advances our understanding of the genetic mechanisms behind prickle formation but also offers practical applications in breeding prickleless eggplant varieties, significantly enhancing agricultural efficiency and reducing the labor required for cultivation and harvesting.

Dr. Lei Zhang, the lead researcher, stated, "This breakthrough in understanding the genetic basis of prickle formation in eggplants is a significant step forward. It not only solves a long-standing agricultural problem but also showcases the power of genetic research in improving crop varieties."

The identification of the PE gene and its association with prickle formation paves the way for developing prickleless eggplant varieties through marker-assisted selection (MAS). This advancement holds promise for making eggplant cultivation and harvesting more efficient and less labor-intensive, ultimately benefiting farmers and the agricultural industry.

###

References

DOI

10.1093/hr/uhae134

Original Source URL

https://doi.org/10.1093/hr/uhae134

Funding information

This work was supported by the National Natural Science Foundation of China (Nos 32102378 and 32101732), the Young Elite Scientist Sponsorship Program by Jiangsu Association for Science and Technology (No. JSTJ-2022-051), the Natural Science Foundation of Xuzhou City (No. KC23053), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.

 

South Africa’s controversial lion farming industry is fueling the illegal international trade in big cat bones



Pensoft Publishers
Lion at a commercial breeding facility in South Africa. 

image: 

Lion at a commercial breeding facility in South Africa. 

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Credit: Roberto Vieto / World Animal Protection.




A new research paper published in the scientific journal Nature Conservation has uncovered concerning activities within South Africa's captive lion industry, shedding light on the urgent need for comprehensive governmental action.

The study by World Animal Protection, conducted through direct interviews with workers at two closed-access lion facilities in South Africa’s North West Province, reveals disturbing practices. These include:

  • The use of legal activities such as commercial captive lion breeding and canned hunting to mask involvement in the illegal international trade of lion and tiger bones.
  • Animal welfare violations including reports of malnutrition, lack of clean water, filthy enclosures and animals being deliberately starved throughout the low-hunting season.
  • Illegal hunting practices such as reports of animals being drugged and hunted within an hour of release in enclosures that violate legal minimum size requirements.
  • Unsafe working conditions including reports of a lack of protective gear for workers when preparing the bodies of hunted animals.
  • Potential shifts towards commercial exploitation of other felid species like tigers which could be used as substitutes for lion bones.
  • Poaching incidents of big cats from commercial captive lion farms by unaffiliated actors, with often only the heads and paws of the animals harvested by the poachers.

Facility workers detailed the use of various strategies, including security cameras, patrols, and messaging apps, to evade detection during inspections. These findings emphasise the complexity and severity of issues within the captive lion industry, necessitating immediate and decisive action.

Lead researcher Dr Angie Elwin, Research Manager at World Animal Protection said: “Our study highlights the troubling reality of South Africa's captive lion industry. Legal activities are being exploited to facilitate illegal trade, and this is compounded by serious animal welfare violations and unsafe conditions for workers. Urgent action is needed to protect lions and people”.

Although the commercial captive breeding and canned hunting of lions remains legal, though poorly regulated in South Africa, the export of lion skeletons - including claws and teeth – was declared unconstitutional by the South African High Court in 2019.

In 2021, the South African Government announced its intention to immediately halt the “domestication and exploitation of lions, and to ultimately close all captive lion facilities in South Africa”.

However, a lack of enforcement of regulations and clarity on the future of the industry, has left a legal grey area, enabling some farms to operate what on the surface appear to be legitimate captive lion breeding and ‘canned’ trophy hunting businesses - but which in reality supply the illegal international big cat bone trade facilitated by organised crime gangs.

In light of these revelations, the study calls for the South African Government to implement a comprehensive and well-managed plan to transition away from current practices in the captive lion industry. Key recommendations include:

  • Full Audit of the Industry: To ensure all commercial captive lion farms are officially registered and compliant with regulations until the industry is phased out.
  • Breeding Moratorium: Establish an immediate moratorium on lion breeding to prevent further growth of the commercial captive lion population.
  • Prevention of Bone Stockpiling: Develop and enforce plans to prevent the accumulation of lion bones, which risks fuelling the illegal international lion bone trade.
  • Phase out Plan: Enact a time bound strategic plan to phase out the captive lion farming industry, ensuring ethical treatment of animals and safety for workers.

Senior researcher Dr Neil D’Cruze, Head of Wildlife Research at World Animal Protection said: "The South African Government must take immediate action to fulfil its public pledge to end the controversial captive lion industry. Without a comprehensive time-bound plan and stringent enforcement, this commercial industry will continue to pose significant legal, animal cruelty, and conservation concerns".

It is estimated that between 8,000-12,000 lions and other big cats, including tigers, are bred and kept in captivity in more than 350 facilities across the country.

This study serves as a crucial call to action for both the South African Government and the international community to address and resolve the complex issues surrounding the captive lion industry.

The publication comes at a time when South African NGO Blood Lions encourages the public to raise their voice by sharing the 2024 World Lion Day “You’re killing them softly” campaign message, aimed at informing tourists and visitors to lion farms about the hidden suffering and cruelty involved, and to sign this petition urging the South African government among others to extend the ban on captive lion breeding and trading to other predators.


Lions at a commercial breeding facility in South Africa.

Credit

Roberto Vieto / World Animal Protection

Prepared lion skeleton.

Credit

Lord Ashcroft KCMG PC.



Original source:

Elwin A, Asfaw E, D'Cruze N (2024) Under the lion’s paw: lion farming in South Africa and the illegal international bone trade. Nature Conservation 56: 1–17. https://doi.org/10.3897/natureconservation.56.124555

 

Weather 'whiplash' in Antarctica may help predict effects of future climate change



Virginia Tech
Jeb Barrett 

image: 

(From left) Ph.D. candidate Sarah N. Power, graduate student Meredith Snyder, and Professor J.E. “Jeb” Barrett, in Taylor Valley, one of the McMurdo Dry Valleys in Antarctica, in January 2023. Power and Snyder, along with Barrett, are among the authors of the Earth’s Future journal article.

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Credit: Photo courtesy of J.E. "Jeb" Barrett.




The McMurdo Dry Valleys of Antarctica live up to their name. The region is one of the driest places on Earth — mountains form a wall around the valleys and prevent melting glacier water from intruding, humidity is extremely low, and no rain was documented in the valleys between the 1960s and the early 2020s.

So when Virginia Tech biological sciences Professor J.E. “Jeb” Barrett learned of a March 2022 day in the McMurdo Dry Valleys that the thermometer rocketed to more than 70 degrees Fahrenheit above its average, he thought examining the extreme weather change would be revealing.

In a paper published Aug. 5 in the American Geophysical Union journal Earth’s Future, Barrett and colleagues reported findings indicating that the March 2022 “weather whiplash” led to record-high death rates for invertebrate organisms that count on surviving winter in an “inactive freeze-dried state.”

The event that triggered Barrett’s work happened on March 18, 2022. On that day, a subtropical river of air in the atmosphere swept over Antarctica, creating an almost instant heat wave on a continent that had already begun freezing into the dark polar night of winter. The temperature jump in the McMurdo Dry Valleys that March paralleled that of the past few days when an Antarctica heat wave has alarmed scientists and others.

“Unfortunately, atmospheric scientists expect more weather anomalies like this in Antarctica,” Barrett said. “Studying the impact of the March 2022 event can help us predict how the ecosystem and creatures in it will respond to future climate changes."

The McMurdo Dry Valleys are especially valuable for scientific research because they may be the only place in Antarctica that have a three-decade-long record of meteorology, stream flow, and soil organisms.

The extreme climate of the McMurdo Dry Valleys limits the organisms that can live there to microscopic or nearly microscopic animals such as rotifers, tardigrades, and nematodes. In March in Antarctica, the equivalent of autumn in the Northern Hemisphere, these animals are transitioning into a dry, frozen status that is critical to their ability to survive the coldest months of the year.

Barrett and the team took advantage of multispectral satellite images to determine how much the March 18 spike in temperatures melted the frozen ground. They examined satellite shots from before March 18, then looked for areas that had darkened on the images immediately after the warm weather.

“Only two things could have resulted in the darker images that we saw: shadows from cloud cover or mountainous topography or the addition of water from melting ice,” Barrett said.

Once they eliminated the possibility of shadows on the satellite images, Barrett and other researchers knew the unusually hot day had resulted in a rapid, massive thaw. The valleys had been swamped. Anything near the surface would no longer be frozen.

In December 2022, researchers collected soil from areas within the valleys and counted the number of live or dead invertebrates. They found a mortality rate of more than 50 percent in areas that had wetted up during the March 2022 event. The only other cases of a mortality rate nearing that have occurred during experiments exploring extreme “freeze-thaw treatments.”

“Our findings from this weather event should be eye-opening for us all,” Barrett said. “As we’ve seen in Southwest Virginia recently as well as in many other U.S. communities, the weather can now change like the flip of a switch. Rapid changes to the environment are difficult for microscopic animals, and they are challenging for people, animals, and plants as well.”

A satellite image of the McMurdo Dry Valleys in Antarctica.

Credit

Photo courtesy of J.E. "Jeb" Barrett.

 

The search for the earliest traces of life on Earth


uOttawa team explores carbon in 3.9-billion-year-old Canadian rocks



University of Ottawa

The search for the earliest traces of life on Earth 

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“Our study focuses on chemical sedimentary rocks found in the Saglek-Hebron. These rocks, among the oldest on Earth, dating back 3.9 billion years, are created through oceanic precipitation”

Jonathan O’Neil

— Associate professor in the Department of Earth and Environmental Sciences

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Credit: University of Ottawa




uOttawa team explores carbon in 3.9-billion-year-old Canadian rocks

The isotopic composition of carbon in iron formations from the Saglek-Hebron Complex in Nunatsiavut (northern Labrador) has been seen as evidence of the earliest traces of life on Earth. But a new study by the University of Ottawa, Carleton University and University College London suggests otherwise.

The study shows that the petrographic, geochemical and spectroscopic features in the graphite (the crystalline form of carbon) found in the Saglek-Hebron chemical sedimentary rocks are in fact “abiotic,” that is, nonliving physical or chemical aspects of an environment or devoid of life. 

This enhances our understanding of how early biomass transformed on Earth, emphasizing the interaction between non-biological processes and ancient life remnants. Studying graphitic materials is key to decoding carbon cycling on the early Earth.

This study is crucial to the search for ancient life on Earth and potentially on neighboring planets. 
 

What the researchers did

Researchers used micro-Raman spectroscopy and revisited the isotopic signatures in these rocks. Their findings show that graphite may come from liquid substances containing carbon, hydrogen and oxygen, probably originating from the breakdown of old organic materials.

“Our study focuses on chemical sedimentary rocks found in the Saglek-Hebron. These rocks, among the oldest on Earth, dating back 3.9 billion years, are created through oceanic precipitation. They include banded iron formations that may have been formed by the activity of bacteria,” explains co-author Jonathan O’Neil, an associate professor in the Department of Earth and Environmental Sciences at uOttawa. 

“They are ideal for studying ancient biological processes. Our study challenges the previous interpretation that the carbon isotopic composition of these rocks is indicative of a biological origin, but their spectroscopic properties rather suggesting abiotic characteristics. This prompts us to reconsider the processes responsible for isotopic signatures and how they could be linked to the action of micro-organisms,” O’Neil adds.

Research over the past year has focused on samples collected in Nunatsiavut during a field campaign in 2016. Petrological characterization was carried out in Ottawa and spectroscopic analysis of graphitic carbon was carried out in London, U.K.

Formed by fluids

“Graphitic carbon from chemical sedimentary rock samples has been studied in three sedimentary rock samples that are nearly 3.9 billion years old. Spectroscopic analysis of this graphitic carbon suggests that it was formed from metamorphic fluids (at temperatures of over 500oC), rather than by processes involving bacterial action,” says O’Neil.

The research shows that graphite in rocks may have formed without organic life, possibly through a carbon-extraction process. The degree of crystallization of the graphite correlates with the rocks’ metamorphism, indicating that metamorphism affects the preservation and change of carbon-based materials.

The study, titled “Abiotic synthesis of graphitic carbons in the Eoarchean Saglek-Hebron metasedimentary rocks,” was published in Nature Communications

AMERIKA

Not just an urban problem: new study reports higher rate of shootings by police across suburbs and rural areas


From 2015-2020, 45% of all incidents of shootings by police occurred in rural areas and 22% in the suburbs, reports a new study in the American Journal of Preventive Medicine


Peer-Reviewed Publication

Elsevier




Ann Arbor, August 8, 2024 – Media coverage of shootings by police typically involve urban incidents, giving the impression that the issue is unique to cities. However, national data built from the Gun Violence Archive tells a different story, showing a higher rate of shootings by police in rural and suburban areas than in cities during 2015-2020. A new study in the American Journal of Preventive Medicine, published by Elsevier, reports on the first nationwide, descriptive analysis of where, how often, and under what circumstances individuals in the US are injured or killed in shootings by police in urban, suburban and rural areas.

Lead investigator Julie A. Ward, PhD, MN, RN, Assistant Professor of Medicine, Health, and Society and Public Policy Studies at Vanderbilt University, explains the underlying rationale for the study, "As a public health nurse, I have seen how physically and emotionally impactful fatal and nonfatal shootings can be. Experiences in times of crisis and in encounters with police carry a lot of weight in shaping personal and community feelings of safety, but the federal government doesn’t require policing agencies to report when officers shoot and injure someone. Before our study, existing research on shootings by police in nonurban areas was very limited. Our systematic examination of the circumstances of incidents across the urban to rural continuum brings to light an overlooked population health concern affecting how people experience public safety across large regions of the US.

The study found that over the six years of data analyzed, 45% of shootings by police occurred in rural zip codes; 22% happened in suburban zip codes.

The findings also show that racial disparities exist across the urban-rural continuum. Black and Native American residents of urban areas were most disproportionately affected, but injury rates were also high among suburban Black residents, rural Black residents, and rural Native American residents. Nationally, these groups’ per capita rates of injury from shootings by police were three-to-five times higher than rates of injury among White residents living in the same rurality designation. Rates of injury to Hispanic residents of urban, suburban, and rural areas were nearly two times higher than non-Hispanic White residents’ rates of injury.

Dr. Ward notes, “This is an equity issue. Public safety should not just be a euphemism for policing. All residents should have access to services to help them stay safe and healthy, including in times of crisis.”

The most common types of policing encounters associated with the shootings were traffic stops, domestic violence events, shots-fired reports, attempts to serve warrants or complete arrests, and incidents involving behavioral health needs. Less than half of shootings in rural zip codes involved shots fired only by local police. Fifty-six percent of these shootings in rural areas involved shots fired by sheriffs’ offices, state police, other agencies, or multiple policing agencies.

Dr. Ward adds, “We often use ‘police’ as shorthand for agencies that engage in law enforcement and other policing activities. But this research makes it clear that if we only focus on improving accountability among local police departments, we will miss a big part of the picture experienced by nonurban residents. This suggests potential for wide-reaching prevention nationally if local and national responses include nonurban jurisdictions and nonurban police and sheriffs’ departments.”

She continues, “Over the past several years, we’ve seen huge growth in the national dialog around police use of force, police accountability systems, and alternative responses. A number of communities have introduced new dispatch services, dedicated behavioral health response teams, co-responder models, and other innovative approaches. To have a broad impact nationally, these potential improvements need to not only focus on urban areas or local police departments.”

 

 

Link found between sociocultural institutions in ethnic enclaves and resident health



UC Irvine-led study used novel measures in Asian American and Hispanic neighborhoods



University of California - Irvine





Irvine, Calif., Aug. 8, 2024 — The number of sociocultural institutions within ethnic enclaves may play a significant role in positively influencing the health of immigrant Asian American and Hispanic populations, according to recent research led by the University of California, Irvine.

 

For the study, published online in the journal Social Science and Medicine, researchers created and validated two novel measures – Asian- and Hispanic-serving sociocultural institutions – to identify the different mechanisms that link majority minority neighborhoods to health outcomes.

 

“Our new measures capture aspects of local economies that may support residents through in-language and culturally appropriate services, employment and social groups that help us estimate how they impact community health,” said corresponding author Brittany Morey, associate professor of health, society and behavior in the Joe C. Wen School of Population & Public Health. “Rather than census data, we used business listings to identify organizations that promote cultural and social identity, including arts, civic, historical, religious, social service and membership.”

 

An online audit of 1,627 businesses within 12 cities was conducted using keyword searches to determine potential Asian- or Hispanic-serving sociocultural institutions and assess their density within census tracts. Exploratory regression analyses showed that a high presence of SCIs may be associated with neighborhood-level health indicators. Researchers discovered a larger percentage of residents in a majority Asian tract who had received an annual checkup and fewer current smokers in both majority Asian and majority Hispanic tracts when there were more SCIs.

 

“Our approach advances methodology in measurement of neighborhood SCIs by capturing data that have been previously overlooked,” Morey said. “Further studies will be conducted to examine the impact that economic resources, social capital and the built environment have on positively influencing community-level well-being. Their potential suggests that support for neighborhood SCIs may lessen health inequities by race and ethnicity.”

 

Other team members included faculty, research scientists, postdoctoral fellows and graduate students from UC Irvine, UC San Francisco, the University of Southern California, Columbia University, Kaiser Permanente Northern California and New York’s Roswell Park Comprehensive Cancer Center. The full list can be found here.

 

This research was supported by the National Cancer Institute under grant numbers R01CA230440, R01CA241125 and U01CA195565.

 

About the University of California, Irvine: Founded in 1965, UC Irvine is a member of the prestigious Association of American Universities and is ranked among the nation’s top 10 public universities by U.S. News & World Report. The campus has produced five Nobel laureates and is known for its academic achievement, premier research, innovation and anteater mascot. Led by Chancellor Howard Gillman, UC Irvine has more than 36,000 students and offers 224 degree programs. It’s located in one of the world’s safest and most economically vibrant communities and is Orange County’s second-largest employer, contributing $7 billion annually to the local economy and $8 billion statewide. For more on UC Irvine, visit www.uci.edu.

 

Media access: Radio programs/stations may, for a fee, use an on-campus studio with a Comrex IP audio codec to interview UC Irvine faculty and experts, subject to availability and university approval. For more UC Irvine news, visit news.uci.edu. Additional resources for journalists may be found at https://news.uci.edu/media-resources.