Decellularized spinach serves as an edible platform for laboratory-grown meat

The veiny skeleton of a spinach leaf shows for the first time it can support the growth of artificial meat, a Boston College researcher reports

BOSTON COLLEGE

Research News

 

IMAGE: THIS DIAGRAM SHOWS THE STEPS BOSTON COLLEGE AND WPI RESEARCHERS TOOK TO ISOLATE AND SEED PRIMARY BOVINE SATELLITE CELLS ON A DECELLULARIZED SPINACH LEAF SCAFFOLD. view more 

CREDIT: FOOD BIOSCIENCE

Chestnut Hill, Mass. (3/31/21) -- Spinach, a cost-efficient and environmentally friendly scaffold, provided an edible platform upon which a team of researchers led by a Boston College engineer has grown meat cells, an advance that may accelerate the development of cultured meat, according to a new report in the advance online edition of the journal Food BioScience.

Stripped of all but its veiny skeleton, the circulatory network of a spinach leaf successfully served as an edible substrate upon which the researchers grew bovine animal protein, said Boston College Professor of Engineering Glenn Gaudette, the lead author of the new study. The results may help increase the production of cellular agriculture products to meet rising demand and reduce environmental costs.

"Cellular agriculture has the potential to produce meat that replicates the structure of traditionally grown meat while minimizing the land and water requirements," said Gaudette, the inaugural chair of BC's new Engineering Department. "We demonstrate that decellularizing spinach leaves can be used as an edible scaffold to grow bovine muscle cells as they develop into meat."

Earlier advances by Gaudette in this area garnered worldwide attention. In 2017, Gaudette and a multi-university team showed that human heart tissue could be cultivated on a spinach leaf scaffold, which was chosen because it offered a natural circulatory system that is nearly impossible to replicate with available scientific tools and techniques.

"In our previous work, we demonstrated that spinach leaves could be used to create heart muscle patches," said Gaudette. "Instead of using spinach to regrow replacement human parts, this latest project demonstrates that we can use spinach to grow meat."

Gaudette said the team, which included Worcester Polytechnic Institute graduate students Jordan Jones and Alex Rebello, removed the plant cells from the spinach leaf and used the remaining vascular framework to grow isolated cow precursor meat cells. The cells remained viable for up to 14 days and differentiated into muscle mass.

"We need environmentally and ethically friendly ways to grow meat in order to feed the growing population," said Gaudette, whose research is supported by New Harvest. "We set out to see if we can use an edible scaffold to accomplish this. Muscle cells are anchorage dependent, meaning they need to grab on to something in order to grow. In the lab, we can use plastic tissue culture plates, but plastic is not edible."

The researchers point out that the successful results will lead to further characterization of the materials and scientific processes to better understand how to meet consumer demand and gauge how large-scale production could be accomplished in accordance with health and safety guidelines.

"We need to scale this up by growing more cells on the leaves to create a thicker steak," said Guadette. "In addition, we are looking at other vegetables and other animal and fish cells."

 Study contributes to our understanding of how cocaine withdrawal affects brain circuits

The results could help clinicians understand addiction and enable people to better manage substance withdrawal

WYSS CENTER FOR BIO AND NEUROENGINEERING

VIDEO: RESEARCHERS HAVE FOUND NEW EVIDENCE OF HOW COCAINE WITHDRAWAL AFFECTS BRAIN CIRCUITS. THEY PREPARED ENTIRE INTACT MOUSE BRAINS AND MADE THEM COMPLETELY TRANSPARENT BY REMOVING LIPIDS THEN IMAGED THEM WITH THE... view more 

CREDIT: WYSS CENTER FOR BIO AND NEUROENGINEERING AND UNIVERSITY OF LAUSANNE

GENEVA, LAUSANNE, 31 March 2021: Cocaine is a highly addictive substance that, in the long term, can have adverse effects on health and wellbeing. There are around 18 million cocaine users globally, according to a UN report. Understanding how cocaine modifies brain networks could reveal potential targets for therapies to treat addiction and other neuropsychological disorders.

A new study published today in the journal Frontiers in Synaptic Neuroscience by a team of researchers from the University of Lausanne and the Wyss Center for Bio and Neuroengineering reveals that during cocaine withdrawal, neurons in a brain area associated with depression connect to neurons embedded in an anti-reward system.

When we receive a reward, neurons fire in the brain's ventral tegmental area (VTA) - a region of the brain that produces dopamine. Dopamine is a chemical messenger that creates feelings of joy or pleasure. It spreads throughout the brain, motivating us to repeat our actions and achieve the feeling of pleasure again, a central facet of addictive behavior. When we miss out on a reward, or stop taking an addictive drug, the lateral habenula - a brain area linked to depression - becomes hyperactive and sends signals to the VTA that results in depressive symptoms.

What researchers did not know, until now, is exactly where the neurons from the lateral habenula extend into the VTA and what they connect to during withdrawal from cocaine. Through a series of experiments, the team discovered that, in mice, the neurons from the lateral habenula synapse mostly to neurons in the VTA that do not produce dopamine, creating a connection to an anti-reward system that may be key in driving adverse behavior.

Manuel Mameli, PhD, Associate Professor in the Department of Fundamental Neuroscience, University of Lausanne said: "We wanted to understand why individuals experience depression when they stop taking a substance like cocaine. We knew that cocaine withdrawal induces changes to brain circuits linked to depression and reward, but our results have now taken us a step further and show that there is a reorganization of brain circuits that drive negative behavior."

The team combined their expertise in sample preparation and advanced microscopy to capture 3D images of entire intact mouse brains. They used viral 'tags' to make the neurons fluoresce, then created completely transparent samples by removing lipids, before imaging with the Wyss Center's custom-built lightsheet microscope. With data analytics and visualization tools, they identified the 3D morphology of specific neurons to understand their anatomical distribution inside the brain.

Stephane Pages, PhD, Scientific Coordinator at the Wyss Center and Senior Research Associate at the University of Geneva said: "The beauty of lightsheet imaging is being able to see which neurons connect to each other in the brain. Our microscope and data analytics capabilities pinpointed the specific neuronal connections between the two major brain areas implicated in drug withdrawal."

The results of the study also show that neurons in the anti-reward circuits not only project to the VTA but also from there into other parts of the brain resulting in a cascade of 'anti-reward' information into areas responsible for decision making and motivation, amongst others. The next step is to dissect the functionality of these non-dopamine, anti-reward, circuits to better understand how they influence behavior.

Neuronal connections between the two major brain areas implicated in drug withdrawal (IMAGE)

CAPTION

Neurons from the lateral habenula (green) mostly connect to an anti-reward network of non-dopamine neurons in the VTA. Dopamine neurons are shown in purple. Although there are some connections between the green neurons from the lateral habenula and purple dopamine neurons, these are not the majority.

CREDIT

Mameli Lab, University of Lausanne

The paper: Output-specific adaptation of habenula-midbrain excitatory synapses during cocaine withdrawal by Joseph Clerke, Patricia Preston-Ferrer, Ioannis S. Zouridis, Audrey Tissot, Laura Batti, Fabian F. Voigt, Stéphane Pagès, Andrea Burgalossi and Manuel Mameli is published in Frontiers in Synaptic Neuroscience, doi 10.3389/fnsyn.2021.643138.

Image caption: Neurons from the lateral habenula (green) mostly connect to an anti-reward network of non-dopamine neurons in the VTA. Dopamine neurons are shown in purple. Although there are some connections between the green neurons from the lateral habenula and purple dopamine neurons, these are not the majority.

Image credit: Mameli Lab, University of Lausanne

About the Wyss Center for Bio and Neuroengineering, Geneva, Switzerland

The Wyss Center is an independent, non-profit research and development organization that advances our understanding of the brain to realize therapies and improve lives.

The Wyss Center staff, together with the Center's academic, clinical and industrial collaborators, pursue innovations and new approaches in neurobiology, neuroimaging and neurotechnology.

Wyss Center advances reveal unique insights into the mechanisms underlying the dynamics of the brain and the treatment of disease to accelerate the development of devices and therapies for unmet medical needs.

The Wyss Center was established by a generous donation from the Swiss entrepreneur and philanthropist Hansjörg Wyss in 2014. Additional resources from funding agencies and other sources help the Wyss Center accelerate its mission. http://www.wysscenter.ch/

The diary of a drug fiend : Crowley, Aleister, 1875-1947 ...

https://archive.org/details/b29826433

2017-10-12 · The diary of a drug fiend by Crowley, Aleister, 1875-1947. n 80057223. Publication date 1922 Topics Crowley, Aleister, 1875-1947, Substance-Related Disorders, Drug abuse, Drug addicts ... PDF download. download 1 file . SINGLE PAGE ORIGINAL JP2 TAR download. download 1 file ...

Weight loss changes people's responsiveness to food marketing: study

UNIVERSITY OF BRITISH COLUMBIA

Research News

 

Obesity rates have increased dramatically in developed countries over the past 40 years -- and many people have assumed that food marketing is at least in part to blame. But are people with obesity really more susceptible to food marketing? And if they are, is that a permanent predisposition, or can it change over time?

According to a new study by UBC Sauder School of Business Assistant Professor Dr. Yann Cornil (he/him/his) and French researchers, people with obesity do tend to be more responsive to food marketing -- but when their weight drops significantly, so does their responsiveness to marketing.

For the study, which was published in the Journal of Consumer Psychology, the researchers followed three groups: patients with severe obesity before they had gastric bypass or other weight-loss surgeries (collectively known as bariatric surgery), as well as three and 12 months after; people with obesity who were not undergoing bariatric surgery; and people who were not obese.

To measure their responsiveness to food marketing, the researchers evaluated what's called framing effects -- that is, how branding, advertising, and labeling "frame," and thus influence food evaluations and choices. In one study, participants were asked to estimate the calorie content in well-known snacks and drinks including some, which marketers typically framed as healthy (i.e. apple juice, granola bars), and others, which are not framed as healthy (i.e. soft drinks, chocolate bars).

The researchers found that everyone underestimated the calorie content of snacks that were framed as healthy but the effect was more pronounced in people with obesity.

To further test the framing effect, the researchers had participants hypothetically choose a portion of french fries from a fast food restaurant, and gave them the nutritional information they would need to make an informed decision. The three options were always the same in quantity -- 71g, 117g, and 154g -- but in one instance they were labeled small, medium and large, and in another instance the same portions were labeled mini, small and medium: a marketing tactic aimed at making larger portions seem more reasonable.

"We measured how likely people were sensitive to that framing, and whether it would change their choice of fries quantity depending on how the portions are labeled," explains Dr. Cornil, who says the people with obesity were more likely to follow the labeling and not the actual information about quantity -- so they would choose the portion labeled "medium" even though that's quite large.

Overall the researchers, who worked closely with the Pitié-Salpêtrière Hospital in Paris, found that the people with obesity tended to be more responsive to food marketing -- but when they lost a significant amount of weight because of bariatric surgery, their level of responsiveness to food marketing dropped substantially.

"People with obesity going through bariatric surgery will become less responsive to marketing over time," says Dr. Cornil. "And after 12 months, their responsiveness to marketing reaches the level of people with more medically-recommended weight."

Dr. Cornil says it's not clear whether people with obesity become less responsive to marketing because of physiological changes following the surgery -- hormonal, neurological shifts or changes to the gut microbiota -- or because of people's desire to change their lifestyles and habits. Another possible reason, he adds, is that people's tastes tend to shift following bariatric surgery.

"The results clearly suggest a bidirectional influence between people's weight status, psychology and responsiveness to the environment -- including marketing," says Dr. Cornil. "So, it's a complex relationship."

However, had the researchers found the responsiveness to marketing remained high even after weight loss, it would have pointed to a deeper-rooted predisposition.

"That would mean people are endowed with unchangeable psychological characteristics that would always make them more responsive to marketing -- which would make it very difficult to sustain a medically-recommended weight," he explains. "But one of the positive things is that after significant weight loss, people become less responsive to marketing, such that it is more sustainable to remain at a lower body mass index."

Dr. Cornil says the findings are especially important because for years, researchers have assumed that marketing messages -- especially for foods that are high-calorie and low in nutrition -- are at least partly responsible for the obesity epidemic, but there wasn't clear empirical evidence.

"Our results provide important insights for policy-makers in charge of regulating food marketing in order to curb obesity," says Dr. Cornil.

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Unique macro-vertebrate at risk from blood sport and climate change

OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY (OIST) GRADUATE UNIVERSITY

Research News

 

IMAGE: AN ARTISTIC DEPICTION OF A KANGASKHAN - A TWO-METER-TALL POKÉMON ENDEMIC TO AUSTRALIA. view more 

CREDIT: IMAGE BY KATERINA ZAPFE

• The kangaskhan, Australia's only species of endemic Pokemon in Pokemon Go, is commonly poached within its natural habitat by Pokemon trainers for use in fighting contests

• Researchers used several species distribution modeling algorithms to predict how climate change, on top of the already existing human-induced pressures, would impact the distribution of the kangaskhan in the future

• In addition to this, they found a way to measure how biased commonly used species distribution models are, and found that some models are so biased that their results weren't influenced by the data at all

• The researchers compared these results to previously published models for hundreds of species of Australian mammals and found similar biases

• This research has highlighted specific problems with common species distribution models, and has given scientists new statistical tools to refine them in the future

Kangaskhan (Garura kangaskhani) are two-meter-tall Pokemon, endemic to Australia. Although commonly spotted around cities and other urban areas, information is lacking about their basic biology and wider range distribution. Much of what is known is based on anecdotes from the public, especially unlicensed breeders, many of whom are not trained in scientific research. Today, kangaskhan are believed to be threatened due to frequent poaching of adults and eggs. The poaching is primarily motivated by the demand for these animals to be used in fighting contests. This pressure, combined with the fact that climate change is predicted to have a large impact in Australia over the next few decades, has resulted in a bleak outlook for this majestic Pokemon species.

Now, researchers in the Biodiversity and Biocomplexity Unit at the Okinawa Institute of Science and Technology Graduate University (OIST) have taken an extensive look at how climate change will impact the existence of suitable habitat for kangaskhan. This study was published today in Methods in Ecology and Evolution.

"Apart from seeming to prefer partly cloudy weather, very little is known about the species' climatic preferences," said lead author Dr. Dan Warren, who previously worked as a researcher in Australia. "This makes it difficult to estimate the response of kangaskhan to environmental change, or how climate change might combine with the effects of poaching to impact the species' long-term survival. For this research, we used several well-known modeling methods to gain insight into how threatened kangaskhan really are."

With data that was initially recorded by hobbyists and professional trainers seeking out kangaskhan for exploitation, the researchers attempted to shed light on what the future holds for this species. Species distribution models were the obvious way to go. These models are a common way for scientists to predict changes in the availability of suitable habitat for a species, in response to estimated shifts in environmental parameters like rainfall, humidity, temperature, and vegetation cover.

In terms of the results, the models differed on what they predicted the future to be for the kangaskhan depending on the algorithms used. Three of the models predicted a decrease in habitat suitability, though differed substantially on the magnitude of this decline. Two other models switched their answer depending on the climate estimates used, and a sixth model predicted an increase in suitability of habitat. Policymakers and stakeholders should take this uncertainty into account and err on the side of caution when it comes to safeguarding the future of kangaskhan.

Aside from revealing uncertainty surrounding the long-term survival of kangaskhan, the research has also shed light on how scientists can calculate the biases that come with species distribution models. Every one of these models comes with a level of bias and uncertainty, depending on the choices made in the modeling process. For example, when predicting the implications of climate change, the amount of future CO2 emissions needs to be considered, and yet estimating this can be tricky as it's based on human behavior. Differences in these estimates can interact with other aspects of the modeling process, resulting in biases in predictions of how climate change will affect threatened and endangered species. Scientists know about these biases but have never found a way to measure them before, until now.

"We found that we could determine the level of bias with a simple statistical test," said Dr. Warren. "And, in doing so, we realized that some of these models could be so biased that the data didn't have much effect on their results... effectively the conclusion was picked based on study design regardless of what the data actually showed," said Dr. Warren.

In addition to the kangaskhan, the researchers demonstrated similar biases for these models by reexamining a previous study that built models for 220 species of Australian mammals, as well as a study that simulated artificial organisms in order to understand how well models estimated species' environmental tolerances. The researchers hope that by revealing these biases they are providing a more secure future for kangaskhan and the rest of the world's biodiversity.

Dr. Warren emphasized that although the study itself might seem strange, what it's revealed will actually go on to help other researchers create more robust estimates of species distribution. His primary reason for choosing to do this research on a Pokemon character was to engage a broader audience with issues that conservation scientists regularly need to think about.

"It's a bit silly but it's also cool science," said Dr. Warren. "Stakeholders use these models for predicting a number of important ecological phenomena, from the effects of climate change, to the dispersal of diseases and invasive species, so having a firm understanding of the biases the models might have is important. We've just scratched the surface of what we can do with this method so I think this could be a useful tool for a whole bunch of things."

His final point was a word of caution with respect to the culture that surrounds Pokemon. "It's specifically based around over-exploitation with the tagline 'gotta catch them all.' The rarer they get, the more valuable they get. This is like some of the larger tuna species, which are in serious danger of going extinct. We might have focused on climate change in this study but, for many Pokemon (and many species around the world), overexploitation should also be a concern."

APRIL FOOLS

 

COVAX

Will US public support donating COVID-19 vaccines to low- and middle-income countries?

A new study led by VCU researchers surveyed 788 people. Among its findings: Older people were less likely to support donating vaccines, as were Republicans ONE IN THE SAME

VIRGINIA COMMONWEALTH UNIVERSITY

Research News

 

The pandemic is affecting every country, but not every country has equal access to the lifesaving COVID-19 vaccines. Recent estimates show that high-income countries -- which have just one-fifth of the global adult population -- have purchased more than half of the world's total vaccine doses, resulting in disparities for low- and middle-income countries.

A new study by researchers at Virginia Commonwealth University investigates a key question: Will the U.S. population support donating part of its COVID-19 vaccine stockpile to less prosperous countries?

"COVID-19 is a true global pandemic that has touched every nation on Earth. Borders closed, economic consequences and an incredible level of human suffering, with now more than half a million people who have died of COVID in the U.S. and many more worldwide," said lead author Jeanine Guidry, Ph.D., an assistant professor in the Richard T. Robertson School of Media and Culture in the College of Humanities and Sciences and director of the Media+Health Lab at VCU. "COVID-19 has shown how interconnected we all are, and to defeat it we will need to work together on a global scale."

For the study, "U.S. Public Support for COVID-19 Vaccine Donation to Low- and Middle-Income Countries during the COVID-19 Pandemic," the researchers conducted a survey of 788 U.S. adults that assessed the support of different population groups for donating vaccines, as well as in what quantity and in what time frame.

It found that older respondents were both less likely to endorse higher levels of COVID-19 vaccine donations and were more likely to want to wait until all in the U.S. who want the vaccine have received it.

"We know that while COVID affects everybody, the majority of the people who die from it are people who are older," Guidry said. "So this finding may reflect that vulnerability."

It also found that respondents who identified as Democrats were more likely to endorse more and faster COVID-19 vaccine donations to low- and middle-income countries than Republicans.

"It is possible that those who identify as Democrats were more likely to support higher levels of donation because it may be connected to a belief in health care as a human right and health care for all," Guidry said.

People without health insurance also were less likely to support donation, and wanted to wait until everyone in the U.S. who wants a vaccine has received it.

The study also found that people who rated higher on the "social dominance orientation" scale were both less likely to endorse higher levels of COVID-19 vaccine donations as well as more likely to want to wait until all in the U.S. who want the vaccine have received it. Social dominance orientation is a personality trait measuring a person's support for social hierarchy and belief that their group is superior to others.

"Social dominance orientation may actually be a fundamental driver of support for vaccine donations during the pandemic," said Paul Perrin, Ph.D., a co-author on the study and an associate professor in the Department of Psychology at VCU. "When people believe in their heart that some individuals are inherently better than others and should therefore be afforded more societal privileges, that is a deep worldview that can color many of their other belief systems."

The study's results also have some encouraging signs, said co-author Bernard Fuemmeler, Ph.D., a professor in the Department of Health Behavior and Policy in the VCU School of Medicine.

"Despite some of the differences we observed with respect to age, party affiliation or social dominance, it is encouraging that, for the most part, there was a majority support for donating as much as 10% of vaccines we have available in the U.S. to other countries," he said. "Despite some hesitancy among a minority of the sample, many we surveyed recognized the importance of closing the gap. Policymakers should be encouraged that proposals to donate the vaccine will be met with acceptance."

The study's findings could be valuable to policymakers, health care providers and public health communication professionals working to persuade and target key segments of the U.S. public to support the donation of vaccines to countries that lack the resources to develop and purchase sufficient quantities.

"Our goal is to provide indications about how we can best communicate to the public. How can we best say: 'OK, we need to [donate vaccines] but we want your buy-in. We want your support,'" Guidry said. "Having support for these decisions is going to be critically important. [This study] provides an important starting point."

###

In addition to Guidry, Perrin and Fuemmeler, the study's authors include VCU researchers Kellie Carlyle, Ph.D., an associate professor in the Department of Health Behavior and Policy; Carrie Miller, Ph.D., a postdoctoral fellow in the Department of Health Behavior and Policy; and Mark Ryan, M.D., associate professor in the Department of Family Medicine; as well as Candace Burton, Ph.D, assistant professor at the University of California at Irvine; Linnea Laestadius, Ph.D., an associate professor in the School of Public Health at the University of Wisconsin-Milwaukee; and Emily Vraga, Ph.D., an associate professor in the Hubbard School of Journalism and Mass Communication at the University of Minnesota.

The study is the second to emerge from the researchers' survey findings. The first study, published in December, analyzed Americans' willingness to get a COVID-19 vaccine with and without emergency use authorization.

The new study will be published in the journal Vaccine, published by Elsevier. DOI: https://doi.org/10.1016/j.vaccine.2021.03.027. Copies of the paper are available to credentialed journalists upon request; please contact Elsevier's newsroom at newsroom@elsevier.com or +31 20 485 2719.

Fast, portable test can diagnose COVID-19 and track variants

The field test, called NIRVANA, can simultaneously detect and sequence SARS-CoV-2, influenza and other viruses

SALK INSTITUTE

Research News

 

IMAGE: THE NIRVANA FIELD-TEST KIT view more 

CREDIT: MO LI/KAUST

LA JOLLA--(March 31, 2021) Clinicians using a new viral screening test can not only diagnose COVID-19 in a matter of minutes with a portable, pocket-sized machine, but can also simultaneously test for other viruses--like influenza--that might be mistaken for the coronavirus. At the same time, they can sequence the virus, providing valuable information on the spread of COVID-19 mutations and variants. The new test, dubbed NIRVANA, was described online today by a multi-institution team of scientists in the journal Med.

"This is a virus detection and surveillance method that doesn't require an expensive infrastructure like other approaches," says Juan Carlos Izpisua Belmonte, co-corresponding author and a professor in Salk's Gene Expression Laboratory. "We can accomplish with one portable test the same thing that others are using two or three different tests, with different machines, to do."

Around the world, more than 100 million people have been infected with SARS-CoV-2, the virus that causes COVID-19. A staggering 500,000 Americans have died from COVID-19 to date. Testing the population is key to stopping the spread of the virus. In addition, tracking the spread of new SARS-CoV-2 variants--some of which could respond differently to treatments or vaccines--is critical.

Today, the standard approach to determining whether a nasal swab is positive for COVID-19 is to run a polymerase chain reaction (PCR) test to detect genetic material from the SARS-CoV-2 virus. If the sample is negative, however, patients and clinicians don't get any information on what might be causing the coronavirus-like symptoms--unless they run separate PCR tests, using different swab samples, for other viruses. And if the sample is positive for SARS-CoV-2, they don't learn which COVID-19 variant a patient is infected with unless another set of tests is run; those require a large and expensive next-generation gene-sequencing machine.

Last summer, Mo Li, an assistant professor of bioscience at King Abdullah University of Science and Technology in Saudi Arabia, was pondering ways he could lend his expertise in genetic engineering and nanopore sequencing to combatting the COVID-19 pandemic. Li, who previously spent six years as a Salk postdoctoral researcher in the Izpisua Belmonte lab, wondered whether a gene-detection approach called isothermal recombinase polymerase amplification (RPA) coupled with real-time nanopore sequencing might be more useful--and faster, cheaper and more portable--than the current COVID-19 testing approach. He teamed up with Izpisua Belmonte to find out.

Unlike PCR, which cycles through lower and higher temperatures to separate DNA strands and copy them, RPA uses proteins--rather than temperature changes--to accomplish the same thing in only 20 minutes. The technology lets researchers copy longer stretches of DNA, and probe for multiple genes at the same time.

"We quickly realized that we could use this technique to not only detect SARS-CoV-2, but other viruses at the same time," says Li.

In the new paper, Li and Izpisua Belmonte describe a small, portable device that can screen 96 samples at the same time using the RPA assay. They call the method NIRVANA, for "nanopore sequencing of isothermal rapid viral amplification for near real-time analysis."

The scientists designed NIRVANA to simultaneously test samples for COVID-19, influenza A, human adenovirus, and non-SARS-CoV-2 human coronavirus. In just 15 minutes, the researchers report, the device begins to report positive and negative results. And within three hours, the device finalizes results on all 96 samples--including the sequences of five regions of SARS-CoV-2 that are particularly prone to accumulate mutations leading to new variants such as the B.1.1.7 variant identified in the UK.

Li and Izpisua Belmonte tested NIRVANA on 10 samples known to be positive for SARS-CoV-2, 60 samples of unknown SARS-CoV-2 status, as well as samples of municipal wastewater harboring the SARS-COV-2 virus and others. In all cases, the assay was able to correctly identify which viruses were present. The sequencing data also allowed them to narrow down the origin of SARS-CoV-2 in positive samples; differentiating strains from China and Europe, for instance.

"The design of this assay is really flexible, so it's not just limited to the examples we've shown," says Li. "We can easily adapt it to tackle another pathogen, even something new and emergent."

With the small size and portability of the NIRVANA workflow, it could be used for fast virus detection at schools, airports or ports, the researchers say. It also could be used to monitor wastewater or streams for the presence of new viruses.

"The pandemic has provided two important lessons: first, test widely and quickly, and second, know your variants. Our NIRVANA method provides a promising solution to these two challenges not only for the current pandemic but also for possible future ones," says Izpisua Belmonte, who holds the Roger Guillemin Chair at Salk. Market analysis would be required to determine whether the initial cost of commercialization--and the constant tweaks to the test needed to make sure it detected new variants or new viruses of interest--are worth it, Belmonte adds.

###

In addition to Izpisua Belmonte and Li, other authors on the study were Concepcion Rodriguez Esteban of Salk; Chongwei Bi, Gerargo Ramos-Mandujano, Sharis Hala, Jinna Xu, Sara Mfarrej, Yeteng Tian and Arnab Pain of King Abdullah University of Science and Technology (KAUST); Estrella Nunez Delicado of UCAM Universidad Católica San Antonio de Murcia; Fadwa Alofi of King Fahad Hospital; Asim Khogeer of Saudi Arabia's Ministry of Health; Anwar Hashem of King Abdulaziz University; and Naif Almontashiri of Taibah University.

The work described in the current paper was supported by a competitive research grant from the King Abdullah University of Science and Technology.

About the Salk Institute for Biological Studies:

Every cure has a starting point. The Salk Institute embodies Jonas Salk's mission to dare to make dreams into reality. Its internationally renowned and award-winning scientists explore the very foundations of life, seeking new understandings in neuroscience, genetics, immunology, plant biology and more. The Institute is an independent nonprofit organization and architectural landmark: small by choice, intimate by nature and fearless in the face of any challenge. Be it cancer or Alzheimer's, aging or diabetes, Salk is where cures begin. Learn more at: salk.edu.

Sarah Everard and the Atlanta shooting are reminders that women are not safe

By Savannah Hopkinson, Opinion writer@savannahmhops  Mar 31, 2021, 

THE DESERT NEWS, UTAH

People gather to view and read inscriptions on floral tributes for Sarah Everard, at the bandstand in Clapham Common, London, on March 16, 2021. A body found in woodland south of London was identified as 33-year old Sarah Everard, who went missing while walking home on March 3, and a serving police officer stands charged with her kidnap and murder. Jonathan Brady, Associated Press

Sarah Everard, a 33-year-old who was abducted off the streets of London, did everything a woman is advised to in order to stay safe in a city — wearing identifiable clothing, staying in busy areas, talking to someone on the phone — but that didn’t stop her from disappearing and her body being discovered days later. Even worse, the alleged perpetrator is a police officer, the very person sworn to protect her from harm.

Then news broke of the shootings in Atlanta where minority women were the primary victims. As the Wall Street Journal put it: “The killings claimed the lives of four women born in Korea, two from China, a mother getting a massage with her husband and a handyman originally from Detroit.”

Twice, the more vicious dangers of misogyny were illuminated and had many women around the country pondering their safety.

Women’s History Month in March was meant to be a celebration of women’s accomplishments and contributions. But it’s hard to feel celebratory each week when the headlines cover a story that put a glaring spotlight on dangers for women.

Earlier this month Aubrey Eyre and I discussed the lingering barriers facing women in the workforce. I’ve commented on the future of women post-COVID-19 and the need for their contributions to economies across the globe

Women were hit hard last year. They’ll also be the key to rebuilding economies post COVID-19

In all of these I have touched on how remaining sexism can be a detriment to female progress, which in turn hinders progress for all of us. But I have not dived deeply into the reality that every woman has had to grapple with the need to constantly look over their shoulder.

No, women do not walk around with wide eyes, clutching their pearls to keep from fainting. On the contrary. Most of us go about our daily routine without thinking about being in danger.

Then we are faced with the reminder that simply being a woman can make you a target. And we remember.

I remember the night a man started riding circles around me on a bicycle when I was walking home from a concert, and it took another group of people coming by and intervening to make him leave.

I remember the first time I was catcalled — when I was just 12 — and the feeling of shame that came with it.

I remember how I changed the route I walked to work just two years ago when a man started waiting on the same corner every morning and would follow me and ask prying questions.

I remember being nervous to walk through the underpass to the train station in the fall and winter, when it was already dark by the time I left the office.

The fear is not always a dominant feeling, but it’s always tethered in the subconscious.

One in 3 women experience sexual violence involving physical contact during their lifetime. That means that even if every woman “does everything right” there is a strong chance she will be a victim. It means that, statistically, each of us know multiple victims.

No, not all men are perpetrators, but it sure seems like it’s just about every woman feels impacted. So how can we tell the difference? I once saw a video that illustrated the point well: Not all bees will sting you and not all spiders or snakes will bite. But the majority of us avoid all bees, snakes and spiders — just in case.

When it could be a family member, a friend, a complete stranger or even a police officer, it feels as though there is no safe place. We can’t blame the victim for getting stung.

Women have been the victims of this warped thinking, and the responsibility for their safety cannot rest on their shoulders alone. We must all work to eradicate the menacing behaviors and thoughts that continue to manifest in brutal ways.

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Where does the problem-solving start? Certainly in the home to a large degree, where children are taught and observe such ideas and behaviors. And in the media, where women are still often portrayed in two-dimensional, stereotypical ways that are a result of the male gaze.

We emulate what we see. If we do not see victims being treated seriously or healthy examples of equality and respect, then how can one expect things to change?

With the right approach, existing problems can still be fixed and future ones can be prevented.

March, as Women’s History Month, was a great reminder of how capable and valuable women have always been, but it was simultaneously an example of how, despite those achievements, there is room for progress.

Sarah Everard was the epitome of the everyday woman — one that we all know and love. A vibrant young marketing executive in a busy city with a loving family, boyfriend and friends. We all know — or are — a Sarah.

Her loss has reignited a movement that, if successful, can make the world better for our own loved ones. For everyday women, we must make every day safer.

There are X-rays coming from Uranus

Image source: NASA, ESA, A. Simon, and M.H. Wong and A. Hsu

By Mike Wehner @MikeWehner
March 31st, 2021 

Uranus is big, cold, and mysterious. As the second-furthest planet from the Sun, it’s rather difficult for researchers here on Earth to study it without sending spacecraft to get a closer look. The only probe to ever make a relatively close flyby of the planet was Voyager 2, and that was over three decades ago. Recently, data beamed back to Earth from NASA’s Chandra X-ray Observatory — a large space telescope that was launched 21 years ago — has helped scientists learn a bit more about the frozen planet.

Using the telescope, which as its name implies is designed to detect X-ray emissions, researchers were able to spot X-ray emissions coming from the planet for the first time. It might sound crazy to think that data gathered back in 2002 was only recently analyzed, but scientists are busy people, and when they finally began comparing images taken from 2002 to 2017 they found clear indications that Uranus was beaming X-rays into space.

In the original images taken in 2002, the scientists say they found “a clear detection” of X-rays, and in the images taken in 2017, they think they might have even spotted an X-ray flare. The image below is the 2002 capture layered over an image of Uranus from a different telescope. The pink you see is the X-ray energy.



Uranus is weird, rotating at a 90-degree angle to all the other planets in our system, but that doesn’t explain the X-rays. Detecting X-ray emissions on Uranus isn’t an entirely unexpected development, as planets like Saturn have been found to do the same. The real question is what is generating that X-ray energy in the first place.

As NASA explains in a new blog post, the biggest source of that energy is likely the Sun itself. Uranus does get sunlight despite being way, way out there, and when that light hits the planet it can scatter. The telescope picks up the wavelengths that it can, and those happen to be X-rays. However, the researchers believe that the Sun isn’t the only source of energy, and it’s possible that the rings around Uranus are helping to produce X-rays of their own.

In a paper published in the Journal of Geophysical Research, a large team of scientists explains how charged particles that surround the planet may be helping to produce X-rays. When those particles interact with the material in the planet’s ring structure, the collisions between the two could be generating X-rays as well. Combined with the X-rays from scattered sunlight, there’s a relatively healthy source of X-ray energy coming from Uranus.

More work needs to be done to confirm this and, ideally, more observations would help. There aren’t currently any plans to visit Uranus on NASA’s short-term roadmap, so for the time being, staring at it from afar is the best we can hope for.