Monday, May 29, 2023

Helium nuclei research advances our understanding of cosmic ray origin and propagation

The latest observations from Low Earth Orbit with the International Space Station provide further evidence of spectral hardening and softening of cosmic ray particles

Peer-Reviewed Publication

WASEDA UNIVERSITY

Measurement of the Cosmic Ray-Helium Spectrum with CALorimetric Electron Telescope 

IMAGE: COSMIC RAY HELIUM PARTICLES WERE FOUND TO FOLLOW A DOUBLE BROKEN POWER LAW, WITH SPECTRAL HARDENING FROM 1.3 TEV AND SOFTENING FROM 30 TEV view more 

CREDIT: WASEDA UNIVERSITY

Much of our understanding of the Universe and its mysterious phenomena is based on theoretical interpretations. In order to deepen the understanding of distant objects and energetic phenomena, astronomers are looking at cosmic rays, which are high-energy charged particles composed of protons, electrons, atomic nuclei, and other subatomic particles. Such studies have revealed that cosmic rays contain all the elements known to us in the periodic table, suggesting that these elements originate from stars and high-energy events such as supernovae. Additionally, due to their charged nature, the path of cosmic rays through space is influenced by the magnetic fields of interstellar phenomena and objects.

Detailed observations of cosmic rays can, thus, not only shed light on the origins of these particles but also decode the existence of high-energy objects and phenomena such as supernova remnants, pulsars, and even dark matter. In an effort to better observe high-energy radiations, Japan, Italy, and USA collaboratively established the CALorimetric Electron Telescope (CALET) on the International Space Station in 2015.

In 2018, observations of the cosmic ray proton spectrum from 50 GeV to 10 TeV revealed that the particle flux of protons at high energies was significantly higher than expected. These results deviated from the conventional cosmic ray acceleration and propagation models that assume a “single power-law distribution,” wherein the number of particles decrease with increasing energy.

Consequently, in a study published in 2022, the CALET team, including researchers from Waseda University, found cosmic ray protons in the energy range of 50 GeV to 60 TeV to follow a “Double Broken Power Law.” This law assumes that the number of high-energy particles initially increase until 10 TeV (known as spectral hardening) and then decrease with an increase in energy (known as spectral softening).

Extending these observations further, the team has now found similar trends of spectral hardening and softening in the cosmic ray helium spectrum captured over a broad range of energy, from 40 GeV to 250 TeV.

The study, published in the journal Physical Review Letters on 27 April, 2023, was led by Associate Professor Kazuyoshi Kobayashi from Waseda University, Japan, along with contributions from Professor Emeritus Shoji Torii, Principal Investigator of the CALET project, also affiliated with Waseda University, and Research Assistant Paolo Brogi from the University of Siena in Italy.

CALET has successfully observed energy spectral structure of cosmic ray helium, especially spectral hardening starting from around 1.3 TeV, and the tendency of softening starting from around 30 TeV,” says Kobayashi.

These observations are based on data collected by CALET aboard the International Space Station (ISS) between 2015 to 2022. Representing the largest energy range to date for cosmic helium nuclei particles, these observations provide additional evidence for deviation of the particle flux from the single power-law model. The researchers noticed that deviation from the expected power-law distribution was more than eight standard deviations away from the mean, indicating a very low probability of this deviation occurring by chance.

Notably, the initial spectral hardening observed in this data suggests that there may be unique sources or mechanisms that are responsible for accelerating and propagating the helium nuclei to high energies. The discovery of these spectral features is also supported by recent observations from the Dark Matter Particle Explorer, and questions our current understanding of the origin and nature of cosmic rays.

These results would significantly contribute to the understanding of cosmic ray acceleration in the supernova remnant and propagation mechanism,” says Torii.

These findings undoubtedly enhance our understanding of the Universe. Even as we prepare for manned missions to the Moon and Mars, the energy distribution of cosmic ray particles can also provide further insight into the radiation environment in space and its effects on astronauts.

 

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100 kW hydrogen fuel cell - digital twin in operation - using green hydrogen and waste plastic hydrogen

Aiming to achieve both carbon neutrality and sustainable economic growth

Business Announcement

TOKYO INSTITUTE OF TECHNOLOGY

Figure.1 Photos of 100 kW hydrogen fuel cell - digital twin 

IMAGE: THE PHOTOS OF THE PLATFORM "100 KW HYDROGEN FUEL CELL - DIGITAL TWIN" FOR OPTIMIZING THE DESIGN AND CONTROL OF A 100 KW HYDROGEN FUEL CELL THAT SEEKS TO BALANCE CARBON NEUTRALITY AND ECONOMIC ADVANTAGE BY MIXING RENEWABLE ENERGY. HYDROGEN/WASTE PLASTIC HYDROGEN. view more 

CREDIT: PROFESSOR MANABU IHARA OF TOKYO INSTITUTE OF TECHNOLOGY

The Tokyo Tech InfoSyEnergy Research and Education Consortium, the Tokyo Tech Academy of Energy and Informatics (Head of Consortium and Academy Director Manabu Ihara, Prof.), and several companies such as Toshiba Corporation and Toshiba Energy Systems & Solutions Corporation are jointly developing a platform "100 kW hydrogen fuel cell - digital twin" for optimizing the design and control of a 100 kw hydrogen fuel cell that seeks to balance carbon neutrality and economic advantage by mixing renewable energy hydrogen/waste plastic hydrogen. They installed the platform in the Tokyo Tech Environmental Energy Innovation (EEI) Building and started operation of the platform.

Hydrogen generated by renewable energy is expected to contribute to carbon neutrality. However, at the current time, introduction is not progressing as expected due to reasons such as the high cost of water electrolyzer and the incompatibility of electrolyzer sizes. Therefore, in order to increase incentives for introducing hydrogen as a technology for carbon neutrality, this system produces hydrogen by supplying electricity from solar cells in the EEI Building to a small-capacity water electrolyzer. It also mixes an appropriate ratio of hydrogen produced from waste plastic (produced by thermal decomposition of waste plastic, steam reforming, shift reaction, and refining process; Resonac Holdings Corporation) and supplies it to the hydrogen fuel cell. Power from the fuel cell will be supplied to the EEI Building and campus, and waste heat will be supplied to the EEI Building's air conditioning system (central heating and cooling) for advanced utilization of waste heat.

Aiming for both carbon neutrality and sustainable economic growth, the system is the first in the world to mix renewable energy hydrogen and waste plastic hydrogen, supply the mixture to a fuel cell, and connect it to the building's air conditioning system for advanced use of electricity and heat. Moving forward, they aim to establish an urban hydrogen energy utilization model that appropriately mixes and optimizes global hydrogen and local hydrogen.

The system is connected to the intelligent energy system Ene-Swallow® that performs peak cut control at the Ookayama Campus of Tokyo Institute of Technology (Tokyo Tech). Its detailed real-time operation data is accumulated in a database and used as energy big data for joint research and education between industry and academia. Furthermore, the mixing ratio of renewable energy hydrogen and waste plastic hydrogen to be supplied to the fuel cell can be controlled in real time from Ene-Swallow®. In the future, the acquisition of detailed data will make it possible to design and control the device capacity of the system. As a carbon neutral digital twin (Ene-Swallow® Digital Twin), a platform that can be integrated and linked with the aim of achieving carbon neutral and sustainable economic growth, they will work for further advancement and promotion as part of R&D for "Carbon-neutral digital twin with the core of energy bigdata" in the JST-MIRAI Program "Advanced Intelligent Information Society" mission area (Program Officer: Eisaku Maeda).

Overview of exhaust heat utilization in 100 kW hydrogen fuel cell system that supplies a mixture of renewable energy hydrogen and waste plastic hydrogen / Overview of connected energy devices Overview of exhaust heat utilization in 100 kW hydrogen fuel cell system that supplies a mixture of renewable energy hydrogen and waste plastic hydrogen / Overview of connected energy devices 

Overview of Ene-Swallow® Digital Twin Overview of Ene-Swallow® Digital Twin 


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About Tokyo Institute of Technology
Tokyo Tech stands at the forefront of research and higher education as the leading university
for science and technology in Japan. Tokyo Tech researchers excel in fields ranging from
materials science to biology, computer science, and physics. Founded in 1881, Tokyo Tech
hosts over 10,000 undergraduate and graduate students per year, who develop into scientific
leaders and some of the most sought-after engineers in industry. Embodying the Japanese
philosophy of “monotsukuri,” meaning “technical ingenuity and innovation,” the Tokyo Tech
community strives to contribute to society through high-impact research.
https://www.titech.ac.jp/english/

WHITE SUPREMACY KILLS

Study finds that eight factors put Black adults at greater risk of early death

The findings show that America's race-based mortality gap is a social, not biological, construct and can be addressed via eight factors

Peer-Reviewed Publication

TULANE UNIVERSITY

Black adults who live in the United States have a 59% higher risk of premature death than White adults.

A new study from Tulane University published in Lancet Public Health has found that this gap can be entirely explained by disparities in eight areas of life critical to health and well-being: employment, income, food security, education level, access to healthcare, quality health insurance, home ownership and marital status.

These eight factors are called social determinants of health. Using data from the National Health and Nutrition Examination Survey, a CDC survey used to determine disease prevalence and risks across the country, Tulane researchers modeled the impact of each factor on a person’s life expectancy. When all unfavorable social determinants were accounted for, the 59% mortality disparity was reduced to zero.

“It totally disappeared,” said Josh Bundy, lead author and epidemiologist at Tulane’s School of Public Health and Tropical Medicine. “There’s no difference between Black and White premature mortality rates after accounting for these social determinants.”

While the mortality gap has been largely pinned on socioeconomic factors such as education level, income and employment status in recent years, researchers have acknowledged that these factors only explained most of the gap, Bundy said.

“This is the first time that anyone completely explained the differences,” Bundy said. “We didn’t expect that, and we were excited about that finding because it suggests social determinants should be the primary targets for eliminating health disparities.”

Socioeconomic factors were still found to play a major role, accounting for approximately 50% of the Black-White difference in mortality in the study. However, the other nearly 50% of the difference was explained by marital status, food security and whether someone has public or private health insurance, softer indicators that can speak to a person’s social support network, stability or job quality. 

Unfavorable social determinants of health were more common among Black adults and were found to carry enormous risk.

Having just one unfavorable social determinant of health was found to double a person’s chances of an early death. With six or more, a person has eight times higher risk of premature mortality.

Jiang He, the corresponding author and Joseph S. Copes Chair of Epidemiology the School of Public Health and Tropical Medicine, said these results “demonstrated that race-based health disparities are social, not biological, constructs.”

Bundy agreed, adding that the findings explain how “structural racism and discrimination lead to worse social risk factors, which may lead to premature death.”

“So how do we eliminate the structural differences between races?” Bundy said. “And regardless of race, if you have six or more of these factors, you’re at a really high risk. How do we address these issues for everyone?”

As a concept, social determinants of health is a relatively new framework being emphasized by the CDC’s Healthy People 2030 initiative.

Going forward, Bundy hopes the concept gains more traction and that policymakers use these findings to address the race-based mortality gap.

“These social determinants of health are the foundation of health problems,” Bundy said. “They need to be a top priority going forward and it’s going to take policy, research and a multi-disciplinary approach to tackle these issues.”

Global flash droughts expected to increase in a warming climate

Peer-Reviewed Publication

UNIVERSITY OF OKLAHOMA

Mena, OK 

IMAGE: A FIGURE SHOWING THE IMPACT OF A FLASH DROUGHT ON A GRASSLAND IN OKLAHOMA. THE PHOTOS ON THE TOP ROW SHOW THE IMPACT OF THE FLASH DROUGHT ON THE ECOSYSTEM COMPARED WITH PHOTOS OF THE SAME AREA WITHOUT FLASH DROUGHT IMPACTS (BOTTOM ROW). view more 

CREDIT: IMAGE PROVIDED BY THE UNIVERSITY OF OKLAHOMA

The rapid development of unexpected drought, called flash drought, can severely impact agricultural and ecological systems with ripple effects that extend even further. Researchers at the University of Oklahoma are assessing how our warming climate will affect the frequency of flash droughts and the risk to croplands globally.

Jordan Christian, a postdoctoral researcher, is the lead author of the study, “Global projections of flash drought show increased risk in a warming climate,” published today in Nature Communications Earth and Environment.

“In this study, projected changes in flash drought frequency and cropland risk from flash drought are quantified using global climate model simulations,” Christian said. “We find that flash drought occurrence is expected to increase globally among all scenarios, with the sharpest increases seen in scenarios with higher radiative forcing and greater fossil fuel usage.”

Radiative forcing describes the imbalance of radiation where more radiation enters Earth’s atmosphere than leaves it. Like burning fossil fuels, these activities are among the most significant contributors to climate warming. The changing climate is expected to increase severe weather events from storms, flash flooding, flash droughts and more.

“Flash drought risk over cropland is expected to increase globally, with the largest increases projected across North America and Europe,” Christian said.

“CMIP6 models projected a 1.5 times increase in the annual risk of flash droughts over croplands across North America by 2100, from the 2015 baseline of a 32% yearly risk in 2015 to 49% in 2100, while Europe is expected to have the largest increase in the most extreme emissions scenario (32% to 53%), a 1.7 times increase in annual risk,” he said.

Jeffrey Basara, an associate professor in the School of Meteorology in the College of Atmospheric and Geographic Sciences and the School of Civil Engineering and Environmental Sciences in the Gallogly College of Engineering, is Christian’s faculty advisor and study co-author. Basara is the executive associate director of the hydrology and water security program and leads OU’s Climate, Hydrology, Ecosystems and Weather research group. The researchers have been investigating ways to improve flash drought identification and prediction since 2017, with multiple papers published in the Journal of HydrometeorologyEnvironmental Research Letters and Nature Communications.

“This study continues to emphasize that agricultural producers, both domestic and abroad, will face increasing risks associated with water availability due to the rapid development of drought. As a result, socioeconomic pressures associated with food production, including higher prices and social unrest, will also increase when crop losses occur due to flash drought,” Basara said.

About the study 

Global projections of flash drought show increased risk in a warming climate,” published in Nature Communications Earth & Environment, May 25, 2023. The National Science Foundation provided funding for the study. In addition to lead author Jordan Christian, co-authors from the University of Oklahoma include professors Jeffrey Basara, Elinor Martin and Jason Furtado in the School of Meteorology as well as Xiangming Xiao in the Department of Biology and Microbiology. Other co-authors include Jason A. Otkin, University of Wisconsin–Madison; Lauren E. L. Lowman, Wake Forest University; Eric D. Hunt, University of Nebraska-Lincoln; and Vimal Mishra, Indian Institute of Technology.

About the University of Oklahoma Office of the Vice President for Research and Partnerships 

The University of Oklahoma is a leading research university classified by the Carnegie Foundation as the nation’s highest tier of research universities. Faculty, staff and students at OU are tackling global challenges and accelerating the delivery of practical solutions that impact society in direct and tangible ways through research and creative activities. OU researchers expand foundational knowledge while moving beyond traditional academic boundaries, collaborating across disciplines and globally with other research institutions as well as decision-makers and practitioners from industry, government and civil society to create and apply solutions for a better world. Find out more at ou.edu/research.

Why high school kids are saying no to alcohol

Peer-Reviewed Publication

UNIVERSITY OF OTAGO

Jude Ball 

IMAGE: DR JUDE BALL. view more 

CREDIT: UNIVERSITY OF OTAGO.

Striking differences in the way high school students socialise may be one of the reasons behind a dramatic drop in youth drinking over the last 20 years, a study from the University of Otago, New Zealand, has found.

Public health researcher Dr Jude Ball has compared attitudes to drinking among high school students in 1999-2001 to those in 2022.

Dr Ball and colleagues Dr Michaela Pettie and Loleseti Poasa interviewed 64 students aged between 14 and 17 at a co-ed school in Wellington in 2022, and compared their views to 41 Christchurch students aged 14-17 who took part in a 1999-2001 study, the Adolescent Friendships and Lifestyles Project.

Their findings are published today in the Asia Pacific journal, Drug and Alcohol Review.

Dr Ball says more than half of those who were in high school 20 years ago were regularly drinking and going to parties by the time they were in Year 10. By Year 12, all had at least some experience of using alcohol with their peers.

“The majority had been drunk at least once or twice and many drank to intoxication on a weekly basis.”

By contrast, only one of the Year 10 students interviewed last year reported drinking alcohol socially.

“Most had never had more than a few sips of alcohol. Even among the students in Years 11 and 12, about three-quarters were abstinent or drank moderately on rare occasions, often with family, rather than with friends.”

She says the reasons behind the change in attitudes are complex, but it seems social media and spending time online is replacing the drinking and partying of teens 20 years ago.

“Parties used to enable young people to expand their social circle, meet potential romantic partners, or take an existing friendship to a romantic or sexual level. Now adolescents can expand their social circle, meet potential romantic partners and try on a more flirtatious and confident persona – all without leaving the house.”

Dr Ball says not drinking is now far more socially acceptable among teens than it used to be.

“Twenty years ago, Year 10 students described a social hierarchy, with early adopters of alcohol at the top and non-drinking ‘nerds’ at the bottom. By Year 12, drinking was seen as an almost compulsory aspect of teen socialising, particularly for males.

“In contrast, non-drinking was the norm for many contemporary adolescents. Although alcohol was part of social life for some friend groups, many teens described active social lives that did not involve the use of alcohol or other substances.”

Dr Ball says a greater acceptance of diversity and more respect for individuals making their own personal choices had removed peer pressure to drink alcohol among contemporary teens.

One Year 11 student commented: “It feels like there aren’t (such) strict unwritten rules for being a teenager … maybe it’s because the internet has made liking different things much more prevalent … I think people are just more understanding of the fact that everyone’s different.”

Some contemporary adolescents regarded drinking or a party lifestyle as a risk to their personal ambitions and an unproductive use of time and money.

One student commented: “I’ve got better things to do than party … I’m looking out for my future here. I can’t have this distraction.”

Dr Ball says there was also a much stronger awareness of alcohol-related risks among the new generation.

“Many mentioned the long-term health risks associated with alcohol, including cancer, liver damage, and the impact of substance use on brain development.”

They were also more likely to worry about the risk of becoming addicted.

“Today’s youth are more likely to regard drinking as a high risk activity with few benefits, while in the past, not drinking alcohol was socially risky.”

Dr Ball says it is unclear whether the changes in the attitudes of 14-17 year olds over the past 20 years reflects a unique generation who will remain ‘dry’ throughout their lives, or just one which is  delaying alcohol use and will ‘catch up’ with previous generations when they reach early adulthood.

“New Zealand evidence suggests binge drinking remains highly prevalent in early adulthood, and despite declining alcohol use in high school students, binge drinking in older adolescents remains much higher here than in other countries like Australia and the US. Policy changes to reduce alcohol harm among young people is still an important public health priority.”

Groundbreaking images of root chemicals offer new insights on plant growth

Technology used in cancer research leads to roadmap of chemicals important for agriculture, food production and climate resilience

Peer-Reviewed Publication

UNIVERSITY OF CALIFORNIA - SAN DIEGO

Testing Plant Roots 

IMAGE: UC SAN DIEGO AND STANFORD SCIENTISTS STUDIED MAIZE (CORN) PLANT ROOTS AND THEIR METABOLITES—MOLECULES INVOLVED IN THE PLANT’S ENERGY PRODUCTION—UNDER DIFFERENT SETTINGS, INCLUDING A CONTROL CONDITION (LEFT) AND TREATED WITH ACONITATE (CENTER) AND SUCCINATE (RIGHT). view more 

CREDIT: DICKINSON LAB, UC SAN DIEGO

On a sunny springtime stroll through a park, it’s easy to ignore the parts of plants that are hidden from view. Plant biologists see things differently. They look below the surface where plant roots are organized in elaborate systems that are critical to the organism’s development. Intricately organized tree root systems, for example, can span as far underground as the tree grows high above the soil.

Applying an advanced imaging technology to plant roots, researchers at the University of California San Diego and Stanford University have developed a new understanding of essential root chemicals that are responsible for plant growth. Using a type of mass spectrometer, a study led by UC San Diego Biological Sciences Postdoctoral Scholar Tao Zhang and Assistant Professor Alexandra Dickinson produced a “roadmap” that profiles where key small molecules are distributed along stem cells of maize (corn) plant roots and how their placement factors into the plant’s maturation. The findings were published in the journal Nature Communications.

“This chemical roadmap provides a resource that scientists can use to find new ways of regulating plant growth,” said Dickinson, a faculty member in the Department of Cell and Developmental Biology. “Having more information about how roots grow could be useful in conservation as we think about protecting our plants in natural environments and making them more sustainable, especially in agriculture.”

While working as a visiting scientist at Stanford University, Dickinson began collaborating with study co-first author Sarah Noll and Professor Richard Zare, who developed a mass spectrometry imaging system that helps surgeons distinguish between cancerous and benign tissue during tumor-removal operations.

Dickinson, Zare and Noll adapted the technology—called “desorption electrospray ionization mass spectrometry imaging” or DESI-MSI—to probe plant roots for the chemicals involved in growth and energy production. They initially focused on maize plants at the root tips, where stem cells play an active role in the plant’s development. Their method involved cutting through the center of the root to get a clear image of the chemicals inside.

“To help understand plant roots from the biology side, we needed to find out which chemicals are there,” said Zare. “Our imaging system sprays out droplets that strike different portions of the root and dissolve chemicals at that location. A mass spectrometer collects the droplet splash and tells us what those dissolved chemicals are. By systematically scanning the droplet target spot we make a spatial map of the root chemicals.”

The resulting images, believed to be some of the first to reveal the transition between stem cells and mature root tissue, show the foundational role of metabolites—molecules involved in the plant’s energy production. Tricarboxylic acid (TCA) cycle metabolites became the focus of the research since they were found to be a key player in controlling root development.

Coming into the study, the researchers expected a relatively uniform distribution of chemicals. Instead, with their chemical roadmap in hand, they found that TCA metabolites are clustered in patches across the root.

“I was surprised by how many chemicals are featured in really distinct patterns,” said Dickinson. “We can see that the plant is doing this on purpose—it needs these molecules in specific regions to grow properly.” The Dickinson lab showed that these TCA metabolites have predictable effects in development, not only in maize, but in another plant species as well (Arabidopsis). This is likely because TCA metabolites are highly conserved—they are made in all plants as well as animals.

Also emerging from the new images were previously unidentified chemical compounds. Dickinson says the mystery compounds could be critical for plant growth since they also are grouped in patterns at specific locations, suggesting a prominent role in development. Dickinson and her colleagues are now investigating these compounds and comparing varieties of maize that have different levels of stress resistance for adverse threats such as severe climate conditions and drought. The new information will help them develop novel chemical and genetic strategies for improving plant growth and stress resilience.

“We’re looking at different maize plants that have drought resistance to see if we’ve already found chemicals that are specific to that variety that we haven’t seen in other varieties,” said Dickinson. “We think that could be a way to find new compounds that can promote growth, especially in harsh conditions.”

The study’s full author list includes: Tao Zhang, Sarah Noll,  Jesus Peng, Amman Klair, Abigail Tripka, Nathan Stutzman, Casey Cheng, Richard Zare and Alexandra Dickinson.


Imaging Plant Root Growth 

Early toilets reveal dysentery in Old Testament Jerusalem

Peer-Reviewed Publication

UNIVERSITY OF CAMBRIDGE

Toilet seat taken from the House of Ahiel 

IMAGE: THE TOILET SEAT TAKEN FROM THE HOUSE OF AHIEL, EXCAVATED IN THE OLD CITY OF JERUSALEM. A DOMESTIC BUILDING MADE UP OF SEVEN ROOMS, IT WOULD HAVE HOUSED AN UPPER-CLASS FAMILY AT THE TIME. DATE OF CONSTRUCTION IS HARD TO PIN DOWN, WITH SOME PLACING IT AROUND THE 8TH CENTURY BCE. view more 

CREDIT: F. VUKASAVOVIC



A new analysis of ancient faeces taken from two Jerusalem latrines dating back to the biblical Kingdom of Judah has uncovered traces of a single-celled microorganism Giardia duodenalis – a common cause of debilitating diarrhoea in humans.

A research team led by the University of Cambridge say it is the oldest example we have of this diarrhoea-causing parasite infecting humans anywhere on the planet. The study is published in the journal Parasitology.  

“The fact that these parasites were present in sediment from two Iron Age Jerusalem cesspits suggests that dysentery was endemic in the Kingdom of Judah,” said study lead author Dr Piers Mitchell from Cambridge’s Department of Archaeology.

“Dysentery is a term that describes intestinal infectious diseases caused by parasites and bacteria that trigger diarrhoea, abdominal cramps, fever and dehydration. It can be fatal, particularly for young children.”

“Dysentery is spread by faeces contaminating drinking water or food, and we suspected it could have been a big problem in early cities of the ancient Near East due to over-crowding, heat and flies, and limited water available in the summer,” said Mitchell.

The faecal samples came from the sediment underneath toilets found in two building complexes excavated to the south of the Old City, which date back to the 7th century BCE when Jerusalem was a capital of Judah.

During this time, Judah was a vassal state under the control of the Assyrian Empire, which at its height stretched from the Levant to the Persian Gulf, incorporating much of modern-day Iran and Iraq. Jerusalem would have been a flourishing political and religious hub estimated to have had between 8,000 and 25,000 residents.

Both toilets had carved stone seats almost identical in design: a shallow curved surface for sitting, with a large central hole for defecation and an adjacent hole at the front for male urination. “Toilets with cesspits from this time are relatively rare and were usually made only for the elite,” said Mitchell.

One was from a lavishly decorated estate at Armon ha-Natziv, surrounded by an ornamental garden. The site, excavated in 2019, probably dates from the days of King Manasseh, a client king for the Assyrians who ruled for fifty years in the mid-7th century.

The site of the other toilet, known as the House of Ahiel, was a domestic building made up of seven rooms, housing an upper-class family at the time. Date of construction is hard to pin down, with some placing it around the 8th century BCE.

However, its destruction is safely dated to 586 BCE, when Babylonian ruler Nebuchadnezzar II brutally sacked Jerusalem for a second time after its citizens refused to pay their agreed tribute, bringing to an end the Kingdom of Judah.


Ancient medical texts from Mesopotamia during the first and second millennium BCE describe diarrhoea affecting the populations of what is now the Near and Middle East. One example reads: “If a person eats bread and drinks beer and subsequently his stomach is colicky, he has cramps and has a flowing of the bowels, setu has gotten him”.

The cuneiform word often used in these texts to describe diarrhoea was sà si-sá. Some texts also included recommended incantations for reciting to increase the chances of recovery.

“These early written sources do not provide causes of diarrhoea, but they encourage us to apply modern techniques to investigate which pathogens might have been involved,” said Mitchell. “We know for sure that Giardia was one of those infections responsible.”

The team investigated the two-and-a-half-thousand year-old decomposed biblical period faeces by applying a bio-molecular technique called “ELISA”, in which antibodies bind onto the proteins uniquely produced by particular species of single-celled organisms.

“Unlike the eggs of other intestinal parasites, the protozoa that cause dysentery are fragile and extremely hard to detect in ancient samples through microscopes without using antibodies,” said co-author and Cambridge PhD candidate Tianyi Wang.

The researchers tested for EntamoebaGiardia and Cryptosporidium: three parasitic microorganisms that are among the most common causes of diarrhoea in humans, and behind outbreaks of dysentery. Tests for Entamoeba and Cryptosporidium were negative, but those for Giardia were repeatedly positive.

Previous research has dated traces of the Entamoeba parasite, which also causes dysentery, as far back as Neolithic Greece over 4,000 years ago. Previous work has also shown that users of ancient Judean toilets were infected by other intestinal parasites including whipworm, tapeworm and pinworm.

This research was undertaken through a collaboration between the University of Cambridge, Tel Aviv University, and the Israel Antiquities Authority.