Monday, November 06, 2023

 

ICYMI

The remains of an ancient planet lie deep within Earth (VIDEO)


Peer-Reviewed Publication

CALIFORNIA INSTITUTE OF TECHNOLOGY

Mysterious giant blobs of material near Earth's core 

VIDEO: 

VISUALIZATION OF THE EARTH WITH LARGE "BLOBS" OF DENSE MATERIAL NEAR EARTH'S CORE. THESE BLOBS WERE DISCOVERED IN THE 1980S. NOW, RESEARCHERS PROPOSE THAT THEY ARE ACTUALLY THE REMNANTS OF AN ANCIENT PLANET, THEIA, THAT COLLIDED WITH EARTH TO FORM THE MOON.

view more 

CREDIT: EDWARD GARNERO




In the 1980s, geophysicists made a startling discovery: two continent-sized blobs of unusual material were found deep near the center of the Earth, one beneath the African continent and one beneath the Pacific Ocean. Each blob is twice the size of the Moon and likely composed of different proportions of elements than the mantle surrounding it.

Where did these strange blobs—formally known as large low-velocity provinces (LLVPs)—come from? A new study led by Caltech researchers suggests that they are remnants of an ancient planet that violently collided with Earth billions of years ago in the same giant impact that created our Moon.

The study, published in the journal Nature on November 1, also proposes an answer to another planetary science mystery. Researchers have long hypothesized that the Moon was created in the aftermath of a giant impact between Earth and a smaller planet dubbed Theia, but no trace of Theia has ever been found in the asteroid belt or in meteorites. This new study suggests that most of Theia was absorbed into the young Earth, forming the LLVPs, while residual debris from the impact coalesced into the Moon.

The research was led by Qian Yuan, O.K. Earl Postdoctoral Scholar Research Associate in the laboratories of both Paul Asimow (MS '93, PhD '97), the Eleanor and John R. McMillan Professor of Geology and Geochemistry; and Michael Gurnis, the John E. And Hazel S. Smits Professor of Geophysics and Clarence R. Allen Leadership Chair, director of Caltech’s Seismological Laboratory, and director of the Schmidt Academy for Software Engineering at Caltech.

Scientists first discovered the LLVPs by measuring seismic waves traveling through the earth. Seismic waves travel at different speeds through different materials, and in the 1980s, the first hints emerged of large-scale three-dimensional variations deep within the structure of Earth. In the deepest mantle, the seismic wave pattern is dominated by the signatures of two large structures near the Earth's core that researchers believe possess an unusually high level of iron. This high iron content means the regions are denser than their surroundings, causing seismic waves passing through them to slow down and leading to the name "large low velocity provinces." 

Yuan, a geophysicist by training, was attending a seminar about planet formation given by Mikhail Zolotov, a professor at Arizona State University, in 2019. Zolotov presented the giant-impact hypothesis, while Qian noted that the Moon is relatively rich in iron. Zolotov added that no trace had been found of the impactor that must have collided with the Earth.

"Right after Mikhail had said that no one knows where the impactor is now, I had a 'eureka moment' and realized that the iron-rich impactor could have transformed into mantle blobs," says Yuan.

Yuan worked with multidisciplinary collaborators to model different scenarios for Theia's chemical composition and its impact with Earth. The simulations confirmed that the physics of the collision could have led to the formation of both the LLVPs and the Moon. Some of Theia's mantle could have become incorporated into the Earth's own, where it ultimately clumped and crystallized together to form the two distinct blobs detectable today at Earth's core–mantle boundary today; other debris from the collision mixed together to form the Moon.

Given such a violent impact, why did Theia's material clump into the two distinct blobs instead of mixing together with the rest of the forming planet? The researchers' simulations showed that much of the energy delivered by Theia's impact remained in the upper half of the mantle, leaving Earth’s lower mantle cooler than estimated by earlier, lower-resolution impact models. Because the lower mantle was not totally melted by the impact, the blobs of iron-rich material from Theia stayed largely intact as they sifted down to the base of the mantle, like the colored masses of paraffin wax in a turned-off lava lamp. Had the lower mantle been hotter (that is, if it had received more energy from the impact), it would have mixed more thoroughly with the iron-rich material, like the colors in a stirred pot of paints.

The next steps are to examine how the early presence of Theia's heterogeneous material deep within the earth might have influenced our planet's interior processes, such as plate tectonics.

"A logical consequence of the idea that the LLVPs are remnants of Theia is that they are very ancient," Asimow says. "It makes sense, therefore, to investigate next what consequences they had for Earth's earliest evolution, such as the onset of subduction before conditions were suitable for modern-style plate tectonics, the formation of the first continents, and the origin of the very oldest surviving terrestrial minerals."

The paper is titled "Moon-forming impactor as a source of Earth's basal mantle anomalies." Qian Yuan is the first author. In addition to Yuan and Asimow, the additional Caltech coauthor is Yoshinori Miyazaki, Stanback Postdoctoral Scholar Research Associate in Comparative Planetary Evolution. Additional coauthors are Mingming Li, Steven Desch, and Edward Garnero (PhD '94) of Arizona State University (ASU); Byeongkwan Ko of ASU and Michigan State University; Hongping Deng of the Chinese Academy of Sciences; Travis Gabriel of the U.S. Geological Survey; Jacob Kegerreis of NASA’s Ames Research Center; and Vincent Eke of Durham University. Funding was provided by the National Science Foundation, the O.K. Earl Postdoctoral Fellowship at Caltech, the U.S. Geological Survey, NASA, and the Caltech Center for Comparative Planetary Evolution.

Simulation of Theia's collision with Earth (VIDEO)

A detailed simulation of Theia crashing into Earth. While the collision was violent, it was not energetic enough to melt the Earth's lower mantle -- meaning that remnants of Theia could be preserved, rather than mixed homogenously in with the Earth's material.

CREDIT

Hongping Deng

 

Breakthrough discovery sheds light on heart and muscle health


Scientists shoot first true-to-life 3D image of the thick filament of mammalian heart muscle


Peer-Reviewed Publication

MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY

Illustration of the interacting thick and thin filaments in the cardiac sarcomere 

IMAGE: 

ILLUSTRATION OF THE INTERACTING THICK AND THIN FILAMENTS IN THE CARDIAC SARCOMERE BASED ON STRUCTURAL CRYO ELECTRON-TOMOGRAPHY DATA.

view more 

CREDIT: MPI OF MOLECULAR PHYSIOLOGY


Atrial fibrillation, heart failure and stroke – hypertrophic cardiomyopathy can lead to many serious health conditions and is a major cause of sudden cardiac death in people younger than 35. „The heart muscle is a central engine of the human body. Of course, it is easier to fix a broken engine, if you know how it is built and how it functions”, says Stefan Raunser. “At the beginning of our muscle research we have successfully visualized the structure of the essential muscle building blocks and how they interact using electron cryo-microscopy. However, these were static images of proteins taken out of the living cell. They only tell us little about how the highly variable, dynamic interplay of muscle components moves the muscle in its native environment”, says Raunser.

Throuh thick and thin

Skeletal and heart muscles contract upon the interaction of two types of parallel protein filaments in the sarcomere: thin and thick. The sarcomere is subdivided in several regions, called zones and bands, in which these filaments are arranged in different ways. The thin filament consists of F-actin, troponin, tropomyosin, and nebulin. The thick filament is formed of myosin, titin and myosin binding protein C (MyBP-C). The latter can form links between the filaments, whereas myosin, the so-called motor protein interacts with the thin filament to generate force and muscle contraction. Alterations in the thick filament proteins are associated with muscle diseases. A detailed picture of the thick filament would be of immense importance for developing therapeutical strategies to cure these diseases, but has been missing so far.

Milestones in muscle research

“If you want to fully understand how the muscle works on the molecular level, you need to picture its components in their natural environment - one of the biggest challenges in biological research nowadays that cannot be tackled by traditional experimental approaches”, says Raunser. To overcome this obstacle his team developed an electron cryo-tomography workflow specifically tailored to the investigation of muscle samples: The scientists flash-freeze mammalian heart muscle samples, produced by the Gautel group in London, at a very low temperature (- 175 °C). This preserves their hydration and fine structure and thus their native state. A focused ion beam (FIB milling) is then applied to thin out the samples to an ideal thickness of around 100 nanometers for the transmission electron microscope, which acquires multiple images as the sample is tilted along an axis. Finally, computational methods reconstruct a three-dimensional picture at high resolution. In recent years, Raunser’s group successfully applied the customized workflow, resulting in two recent groundbreaking publications: They produced the first high-resolution images of the sarcomere and of a so far nebulous muscle protein called nebulin. Both studies provide unprecedented insights into the 3D organization of muscle proteins in the sarcomere, e. g. how myosin binds to actin to control muscle contraction and how nebulin binds to actin to stabilize it and to determine its length.

Completing the painting

In their current study the scientists produced the first high-resolution image of the cardiac thick filament spanning across several regions in the sarcomere. “With 500 nm length this makes for the longest and biggest structure ever resolved by cryo-ET”, says Davide Tamborrini from the MPI Dortmund, first-author of the study. Even more impressive are the newly gained insights into the thick filament’s molecular organization and thus into its function. The arrangement of the myosin molecules depends on their position in the filament. The scientists suspect, that this allows the thick filament to sense and process numerous muscle-regulating signals and thus to regulate the strength of muscle contraction depending on the sarcomere region. They also revealed how titin chains run along the filament. Titin chains intertwine with myosin, acting as a scaffold for its assembly and probably orchestrating a length-depending activation of the sarcomere.

“Our aim is to paint a complete picture of the sarcomere one day. The image of the thick filament in this study is ‘only’ a snapshot in the relaxed state of the muscle. To fully understand how the sarcomere functions and how it is regulated, we want to analyze it in different states e. g. during contraction“, says Raunser. Comparison with samples from patients with muscle disease will ultimately contribute to a better understanding of diseases like hypertrophic cardiomyopathy and to the development of innovative therapies.

Thick filament structure in the relaxed cardiac sarcomere. The upper image shows a tomographic slice of a cardiac sarcomere. Thin filaments are marked with a green and thick filaments with a purple arrow. The middle image shows the reconstructed thick (purple) and thin (green) filaments. The lower image shows the structure of the thin filament spanning across several sarcomere regions. Scale bar shows 50 nm.

The ringed seals in Ilulissat Icefjord, Greenland are special


Local hunters in the Icefjord near Ilulissat have long known about a special ringed seal – the Kangia seal – which is significantly larger and has a markedly different fur colour and pattern than typical Arctic ringed seals.


Peer-Reviewed Publication

AARHUS UNIVERSITY




Exploring Arctic nature can be difficult. Harsh conditions and great distances are significant challenges when researchers want to coax secrets out of nature.

However, a research project, led by Greenlandic and Danish researchers, has now succeeded in describing a new type of ringed seal that lives in the Icefjord near Ilulissat in West Greenland; a unique natural area on the UNESCO World Heritage List.

The results have just been published in the renowned scientific journal Molecular Ecology.

A small population

Over a number of years, the researchers together with local hunters captured seals in nets and mounted a small satellite transmitter on the seals’ backs. When the seals were up for air, the satellite transmitter sent a message about their location.

”We could see that the Kangia seals primarily stay inside the Icefjord. We were able to count the seals from a plane and therefore able to estimate that there are only approx. 3,000 of these special Kangia ringed seals,” says Aqqalu Rosing-Asvid, Senior Researcher at the Pinngortitaleriffik – Greenland Institute of Nature, and one of the researchers behind the study.

The small resident population is highly unusual compared to the typical Arctic ringed seal, which has an enormous population size and often travels thousands of kilometres around the Arctic in search of food.

Isolated for thousands of years

The researchers also took small tissue samples from the captured seals. The samples were sent for genetic analyses to uncover the seals’ DNA profile, and the results revealed that the Kangia ringed seals are genetically different from the typical Arctic ringed seal.

But where and how the Kangia ringed seal was isolated from the other Arctic ringed seals and why it acquired its new special biological characteristics is still a mystery.

Perhaps also special seals in other Arctic fjords

The study emphasises that there is still much we do not know about the diversity of organisms in the Arctic and thus their possibilities to adapt to climate change and human activities.

“There are many other fjords in the Arctic that have not yet been studied in detail, and where the ringed seals may also have locally developed new genetic variants,” points out Rune Dietz, Professor at the Department of Ecoscience at Aarhus University, who also participated in the study.

 

First mice engineered to survive COVID-19 like young, healthy humans


Model offers new ways to study disease impact and treatment approaches


Peer-Reviewed Publication

NYU LANGONE HEALTH / NYU GROSSMAN SCHOOL OF MEDICINE




Researchers have genetically engineered the first mice that get a human-like form of COVID-19, according to a study published online November 1 in Nature.

Led by researchers from NYU Grossman School of Medicine, the new work created lab mice with human genetic material for ACE2 – a protein snagged by the pandemic virus so it can attach to human cells as part of the infection. The mice with this genetic change developed symptoms similar to young humans infected with the virus causing COVID-19, instead of dying upon infection as had occurred with prior mouse models.

“That these mice survive creates the first animal model that mimics the form of COVID-19 seen in most people – down to the immune system cells activated and comparable symptoms,” said senior study author Jef Boeke, the Sol and Judith Bergstein Director of the Institute for Systems Genetics at NYU Langone Health. “This has been a major missing piece in efforts to develop new drugs against this virus.” 

“Given that mice have been the lead genetic model for decades,” added Boeke, “there are thousands of existing mouse lines that can now be crossbred with our humanized ACE2 mice to study how the body reacts differently to the virus in patients with diabetes or obesity, or as people age.”

Problem of Large DNA

The new study revolves around a new method to edit DNA, the 3 billion “letters” of the genetic code that serve as instructions for building our cells and bodies.

While famous techniques like CRISPR enable the editing of DNA editing just one or a few letters at a time, some challenges require changes throughout genes that can be up to 2 million letters long. In such cases, it may be more efficient to build DNA from scratch, with far-flung changes made in large swaths of code pre-assembled and then swapped into a cell in place of its natural counterpart. Because human genes are so complex, Boeke’s lab first developed its “genome writing” approach in yeast, one-celled fungi that share many features with human cells but that are simpler and easier to study.

More recently, Boeke’s team adapted their yeast techniques to the mammalian genetic code, which is made up of not just of genes that encode proteins, but also of many switches that turn on different genes at different levels in different cell types. By studying this poorly understood “dark matter” that regulates genes, the research team was able to design living mice with cells that had more human-like levels of ACE gene activity for the first time. The study authors used yeast cells to assemble DNA sequences of up to 200,000 letters in a single step, and then delivered these “naked” DNAs into mouse embryonic stem cells using their new delivery method, mSwAP-In.

Overcoming the size limits of past methods, mSwAP-In delivered a humanized mouse model of COVID-19 pathology by “overwriting” 72 kilobases (kb) of mouse Ace2 code with 180 kb of the human ACE2 gene and its regulatory DNA.

To accomplish this cross-species swap, the study method cut into a key spot in the DNA code around the natural gene, swapped in a synthetic counterpart in steps, and with each addition, added a quality control mechanism so that only cells with the synthetic gene survived. The research team then worked with Sang Yong Kim at NYU’s Rodent Genome Engineering Lab using a stem cell technique called “tetraploid complementation” to create a living mouse whose cells included the overwritten genes.

In addition, the researchers had previously designed a synthetic version of the gene Trp53, the mouse version of the human gene TP53, and swapped it into mouse cells. The protein encoded by this gene coordinates the cell’s response to damaged DNA, and can even instruct cells containing it to die to prevent the build-up of cancerous cells. When this “guardian of the genome” itself becomes faulty, it is a major contributor to human cancers.  

Whereas the ACE2 experiments had swapped in an unchanged version of a human gene, the synthetic, swapped-in Trp53 gene had been designed to no longer include a combination of molecular code letters – cytosine (C) next to guanine (G) – known to be vulnerable to random, cancer-causing changes. The researchers overwrote key CG “hotspots” with code containing a different DNA letter in adenine (A).

“The AG switch left the gene’s function intact, but lessened its vulnerability to mutation, with the swap predicted to lead to a 10-to-50 fold lower mutation rate,” said first author Weimin Zhang, PhD, a post-doctoral scholar in Boeke’s lab. “Our goal is to demonstrate in a living test animal that this swap leads to fewer mutations and fewer resulting tumors, and those experiments are being planned.”

***

Along with Boeke and Zhang, NYU Langone study authors were Ran Brosh, Aleksandra Wudzinska, Yinan Zhu, Noor Chalhoub, Emily Huang, and Hannah Ashe in the Institute for Systems Genetics and Department of Biochemistry & Molecular Pharmacology; Ilona Golynker, Lucia Carrau, Payal Damani-Yokota, Camille Khairallah, Kamal Khanna, and Benjamin tenOever in the Department of Microbiology; and Matthew Maurano and Sang Yong Kim in the Department of Pathology.

The work was funded by National Institutes of Health CEGS grant 1RM1HG009491 and Perlmutter Cancer Center Support Grant P30CA016087. Boeke is a founder of CDI Labs, Inc., a founder of Neochromosome, Inc.; a founder of ReOpen Diagnostics, LLC, and serves or has served on the scientific advisory boards of Logomix Inc., Modern Meadow, Inc., Rome Therapeutics, Inc., Sample6, Inc., Sangamo, Inc., Tessera Therapeutics, Inc. and the Wyss Institute. Boeke also receives consulting fees and royalties from OpenTrons, and holds equity in the company. These relationships are managed in accordance with the policies of NYU.

 

In a surprising finding, light can make water evaporate without heat


A newly identified process could explain a variety of natural phenomena and enable new approaches to desalination.


Peer-Reviewed Publication

MASSACHUSETTS INSTITUTE OF TECHNOLOGY



Evaporation is happening all around us all the time, from the sweat cooling our bodies to the dew burning off in the morning sun. But science’s understanding of this ubiquitous process may have been missing a piece all this time.

In recent years, some researchers have been puzzled upon finding that water in their experiments, which was held in a sponge-like material known as a hydrogel, was evaporating at a higher rate than could be explained by the amount of heat, or thermal energy, that the water was receiving. And the excess has been significant — a doubling, or even a tripling or more, of the theoretical maximum rate.

After carrying out a series of new experiments and simulations, and reexamining some of the results from various groups that claimed to have exceeded the thermal limit, a team of researchers at MIT has reached a startling conclusion: Under certain conditions, at the interface where water meets air, light can directly bring about evaporation without the need for heat, and it actually does so even more efficiently than heat. In these experiments, the water was held in a hydrogel material, but the researchers suggest that the phenomenon may occur under other conditions as well.

The findings are published this week in a paper in PNAS, by MIT postdoc Yaodong Tu, professor of mechanical engineering Gang Chen, and four others.

The phenomenon might play a role in the formation and evolution of fog and clouds, and thus would be important to incorporate into climate models to improve their accuracy, the researchers say. And it might play an important part in many industrial processes such as solar-powered desalination of water, perhaps enabling alternatives to the step of converting sunlight to heat first.

The new findings come as a surprise because water itself does not absorb light to any significant degree. That’s why you can see clearly through many feet of clean water to the surface below. So, when the team initially began exploring the process of solar evaporation for desalination, they first put particles of a black, light-absorbing material in a container of water to help convert the sunlight to heat.

Then, the team came across the work of another group that had achieved an evaporation rate double the thermal limit — which is the highest possible amount of evaporation that can take place for a given input of heat, based on basic physical principles such as the conservation of energy. It was in these experiments that the water was bound up in a hydrogel. Although they were initially skeptical, Chen and Tu starting their own experiments with hydrogels, including a piece of the material from the other group. “We tested it under our solar simulator, and it worked,” confirming the unusually high evaporation rate, Chen says. “So, we believed them now.” Chen and Tu then began making and testing their own hydrogels.

They began to suspect that the excess evaporation was being caused by the light itself —that photons of light were actually knocking bundles of water molecules loose from the water’s surface. This effect would only take place right at the boundary layer between water and air, at the surface of the hydrogel material — and perhaps also on the sea surface or the surfaces of droplets in clouds or fog.

In the lab, they monitored the surface of a hydrogel, a JELL-O-like matrix consisting mostly of water bound by a sponge-like lattice of thin membranes. They measured its responses to simulated sunlight with precisely controlled wavelengths.

The researchers subjected the water surface to different colors of light in sequence and measured the evaporation rate. They did this by placing a container of water-laden hydrogel on a scale and directly measuring the amount of mass lost to evaporation, as well as monitoring the temperature above the hydrogel surface. The lights were shielded to prevent them from introducing extra heat. The researchers found that the effect varied with color and peaked at a particular wavelength of green light. Such a color dependence has no relation to heat, and so supports the idea that it is the light itself that is causing at least some of the evaporation.

The researchers tried to duplicate the observed evaporation rate with the same setup but using electricity to heat the material, and no light. Even though the thermal input was the same as in the other test, the amount of water that evaporated never exceeded the thermal limit. However, it did so when the simulated sunlight was on, confirming that light was the cause of the extra evaporation.

Though water itself does not absorb much light, and neither does the hydrogel material itself, when the two combine they become strong absorbers, Chen says. That allows the material to harness the energy of the solar photons efficiently and exceed the thermal limit, without the need for any dark dyes for absorption.

Having discovered this effect, which they have dubbed the photomolecular effect, the researchers are now working on how to apply it to real-world needs. They have a grant from the Abdul Latif Jameel Water and Food Systems Lab to study the use of this phenomenon to improve the efficiency of solar-powered desalination systems, and a Bose Grant to explore the phenomenon’s effects on climate change modeling.

Tu explains that in standard desalination processes, “it normally has two steps: First we evaporate the water into vapor, and then we need to condense the vapor to liquify it into fresh water.” With this discovery, he says, potentially “we can achieve high efficiency on the evaporation side.” The process also could turn out to have applications in processes that require drying a material.

Chen says that in principle, he thinks it may be possible to increase the limit of water produced by solar desalination, which is currently 1.5 kilograms per square meter, by as much as three- or fourfold using this light-based approach. “This could potentially really lead to cheap desalination,” he says.

Tu adds that this phenomenon could potentially also be leveraged in evaporative cooling processes, using the phase change to provide a highly efficient solar cooling system.

Meanwhile, the researchers are also working closely with other groups who are attempting to replicate the findings, hoping to overcome skepticism that has faced the unexpected findings and the hypothesis being advanced to explain them. 

The research team also included Jiawei Zhou, Shaoting Lin, Mohammed Alshrah, and Xuanhe Zhao, all in MIT’s Department of Mechanical Engineering.

###

Written by David L. Chandler, MIT News Office

Paper: “Plausible photomolecular effect leading to water evaporation exceeding the thermal limit.”

https://www.pnas.org/doi/full/10.1073/pnas.2312751120

  I F...KING💓SCIENCE

What happens when we pass out? Researchers ID new brain and heart connections


Neurobiologists discover sensory neurons that control fainting, laying a foundation for targeted treatments for related disorders


Peer-Reviewed Publication

UNIVERSITY OF CALIFORNIA - SAN DIEGO

Heart stimulation 

IMAGE: 

AN IMAGE OF A HEART LABELED BY VAGAL SENSORY NEURONS. IN A NEW STUDY PUBLISHED IN THE JOURNAL NATURE, UC SAN DIEGO RESEARCHERS AND THEIR COLLEAGUES FOUND THAT THESE NEURONS TRIGGER FAINTING, LAYING A FOUNDATION FOR ADDRESSING FAINTING-RELATED DISORDERS.

view more 

CREDIT: AUGUSTINE LAB, UC SAN DIEGO




Nearly 40 percent of people experience syncope, or fainting spells, at least once in their lives. These brief losses of consciousness, whether brought by pain, fear, heat, hyperventilation or other causes, account for a significant portion of hospital emergency room visits. Yet the exact root mechanisms at play when people “pass out” largely have remained a mystery.

Publishing a new report in Nature, University of California San Diego researchers, along with colleagues at The Scripps Research Institute and other institutions, have for the first time identified the genetic pathway between the heart and brain tied to fainting.

One of their unique approaches was to think of the heart as a sensory organ rather than the longstanding viewpoint that the brain sends out signals and the heart simply follows directions. School of Biological Sciences Assistant Professor Vineet Augustine, the paper’s senior author, applies a variety of approaches to better understand these neural connections between the heart and brain.

“What we are finding is that the heart also sends signals back to the brain, which can change brain function,” said Augustine. Information resulting from the study could be relevant to better understanding and treating various psychiatric and neurological disorders linked with brain-heart connections, the researchers note in their paper. “Our study is the first comprehensive demonstration of a genetically defined cardiac reflex, which faithfully recapitulates characteristics of human syncope at physiological, behavioral and neural network levels.”

Augustine, along with Biological Sciences Staff Research Associate Jonathan Lovelace and Graduate Student Jingrui Ma, the first authors of the paper, and their colleagues studied neural mechanisms related to Bezold-Jarisch reflex (BJR), a cardiac reflex first described in 1867. For decades researchers have hypothesized that the BJR, which features reduced heart rate, blood pressure and breathing, may be associated with fainting. But information lacked in proving the idea since the neural pathways involved in the reflex were not well known.

The researchers focused on the genetics behind a sensory cluster known as the nodose ganglia, which are part of the vagus nerves that carry signals between the brain and visceral organs, including the heart. Specifically, vagal sensory neurons, or VSNs, project signals to the brainstem and are thought to be associated with BJR and fainting. In their search for a novel neural pathway they discovered that VSNs expressing the neuropeptide Y receptor Y2 (known as NPY2R) are tightly linked to the well-known BJR responses.

Studying this pathway in mice, the researchers were surprised to find that when they proactively triggered NPY2R VSNs using optogenetics, a method of stimulating and controlling neurons, mice that had been freely moving about immediately fainted. During these episodes they recorded from thousands of neurons in the brains of the mice, as well as heart activity and changes in facial features including pupil diameter and whisking. They also employed machine learning in several ways to analyze the data and pinpoint features of interest. Once NPY2R neurons were activated, they found, mice exhibited rapid pupil dilation and the classic “eye-roll” seen during human fainting, as well as suppressed heart-rate, blood pressure and breathing rate. They also measured reduced blood flow to the brain, an area of collaboration with Professor David Kleinfeld’s laboratory in the UC San Diego Departments of Neurobiology and Physics.

“We were blown away when we saw how their eyes rolled back around the same time as brain activity rapidly dropped,” the researchers reported in a paper summary. “Then, after a few seconds, brain activity and movement returned. This was our eureka moment.”

Further testing showed that when NPY2R VSNs were removed from mice, the BJR and fainting conditions vanished. Previous studies had shown that fainting is caused by a reduction in brain blood flow, which the new study also found to be true, but the new evidence indicated that brain activity itself could be playing an important role. The findings therefore implicate the activation of the newly genetically identified VSNs and their neural pathways not only with BJR, but more centrally in overall animal physiology, certain brain networks and even behavior.

Such findings were difficult to tease out previously because neuroscientists study the brain and cardiologists study the heart, but many do so in isolation of the other. “Neuroscientists traditionally think the body just follows the brain, but now it is becoming very clear that the body sends signals to the brain and then the brain changes function,” said Augustine.

As a result of their findings, the researchers would like to continue tracking the precise conditions under which vagal sensory neurons are triggered into action.

“We also hope to more closely examine cerebral blood flow and neural pathways in the brain during the moment of syncope, to better understand this common but mysterious condition,” they note.

They also hope to use their research as a model to develop targeted treatments for fainting-associated conditions.

The coauthors of the Nature paper include: Jonathan Lovelace, Jingrui Ma, Saurabh Yadav, Karishma Chhabria, Hanbing Shen, Zhengyuan Pang, Tianbo Qi, Ruchi Sehgal, Yunxiao Zhang, Tushar Bali, Thomas Vaissiere, Shawn Tan, Yuejia Liu, Gavin Rumbaugh, Li Ye, David Kleinfeld, Carsen Stringer and Vineet Augustine.

Heart slowing down (VIDEO)

 

University of Cincinnati study: Strawberry consumption may reduce dementia risk for middle-aged individuals


Research published in the journal Nutrients

Peer-Reviewed Publication

UNIVERSITY OF CINCINNATI

Robert Krikorian 

IMAGE: 

ROBERT KRIKORIAN, PHD.

view more 

CREDIT: PHOTO/UNIVERSITY OF CINCINNATI




New research from the University of Cincinnati found that daily strawberry consumption could help reduce the risk of dementia for certain middle-aged populations.

The research was recently published in the journal Nutrients.

Research background

In 2022, UC’s Robert Krikorian, PhD, and his team published research that found adding blueberries to the daily diets of certain middle-aged populations may lower the chances of developing late-life dementia. He said the current research into strawberries is an extension to the blueberry research.

“Both strawberries and blueberries contain antioxidants called anthocyanins, which have been implicated in a variety of berry health benefits such as metabolic and cognitive enhancements,” said Krikorian, professor emeritus in the UC College of Medicine’s Department of Psychiatry and Behavioral Neuroscience. “There is epidemiological data suggesting that people who consume strawberries or blueberries regularly have a slower rate of cognitive decline with aging.” 

In addition to containing anthocyanins, Krikorian said strawberries contain additional micronutrients called ellagitannins and ellagic acid that have been associated with health benefits.

About 50% of individuals in the U.S. develop insulin resistance, commonly referred to as prediabetes, around middle age, which has been shown to be a factor in chronic diseases. Krikorian said the metabolic and cardiovascular benefits of strawberry consumption have been studied previously, but there were relatively few studies on its cognitive effects.

“This study assessed whether strawberry consumption might improve cognitive performance and metabolic health in this population and, if so, whether there might be an association between cognitive enhancement and reduced metabolic disturbance,” he said.

Research methodology

A total of 30 overweight patients between 50-65 years old with complaints of mild cognitive decline were enrolled and completed the study. Krikorian said this population has an increased risk for late-life dementia and other common conditions. 

Over a period of 12 weeks, the participants were asked to abstain from berry fruit consumption of any kind except for a daily packet of supplement powder to be mixed with water and consumed with breakfast. Half of the participants received powders that contained the equivalent of one cup of whole strawberries (the standard serving size), while the other half received a placebo. 

The participants were given tests that measured certain cognitive abilities like long-term memory. The researchers also tracked their mood, intensity of depressive symptoms and metabolic data over the course of the study.

Those in the strawberry powder group had diminished memory interference, which is consistent with an overall improvement in executive ability. 

“Reduced memory interference refers to less confusion of semantically related terms on a word-list learning test,” Krikorian said. “This phenomenon generally is thought to reflect better executive control in terms of resisting intrusion of non-target words during the memory testing.” 

The strawberry-treated participants also had a significant reduction of depressive symptoms, which Krikorian said can be understood as a result from “enhanced executive ability that would provide better emotional control and coping and perhaps better problem-solving.”

Other strawberry studies have found improvement in metabolic measures including lower insulin, but there was no effect found on the patients’ metabolic health in this study.

“Those studies generally used higher dosages of strawberry powder than in our research, and this could have been a factor,” Krikorian said.

Next steps

While more research is needed, Krikorian said the strawberry treatment may have improved cognitive function by reducing inflammation in the brain.

“Executive abilities begin to decline in midlife and excess abdominal fat, as in insulin resistance and obesity, will tend to increase inflammation, including in the brain,” he said. “So, one might consider that our middle-aged, overweight, prediabetic sample had higher levels of inflammation that contributed to at least mild impairment of executive abilities. Accordingly, the beneficial effects we observed might be related to moderation of inflammation in the strawberry group.”

Moving forward, Krikorian said future research trials should include larger samples of participants and differing dosages of strawberry supplementation.

This research was supported with funding and donation of strawberry and placebo powders by the California Strawberry Commission, Watsonville, California 95076, USA. The research authors declare no conflict of interest. The funding organization had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript or in the decision to publish the results.