Showing posts sorted by date for query biofuel. Sort by relevance Show all posts
Showing posts sorted by date for query biofuel. Sort by relevance Show all posts

Wednesday, January 21, 2026

Maritime Leaders Convene in Singapore on FuelEU Strategy and Crew Welfare

OceanOpt

Published Jan 20, 2026 8:00 PM by The Maritime Executive


[By: OceanOpt]

OceanOpt, a provider of End-to-End Maritime Emissions & Operations Intelligence, gathered 40 maritime leaders in Singapore for a critical fireside chat, "Finishing the Year with Confidence." Executives from BSM, DNV, Stephenson Harwood, and CMB.TECH joined the discussion, dissecting how strategic advisory and unified data governance can turn regulatory complexity into a competitive edge. With the industry facing tight FuelEU Maritime deadlines, the conversation quickly moved past generalities to the specific operational realities of compliance and crew welfare.

The Human Cost of Compliance Capt. Graciano Ausan, a recently retired Master Mariner, offered a stark reality check from the front lines. "We are the frontline of maritime operations," he noted. "Yet seafarers report the same fuel data to four separate platforms simultaneously."

He revealed that on some vessels, fuel reporting alone consumes over 90 minutes daily—time stolen from essential maintenance and safety tasks. "This is time diverted
from maintenance and safety," Ausan warned.

Legal Voids and Liability This operational friction is compounded by legal ambiguity. Rachel Hoyland of Stephenson Harwood LLP warned that current BIMCO clauses have not kept pace with FuelEU complexities.

"Biofuel consumption clarity, pool liability allocation, and settlement mechanics remain undefined across most charters," Hoyland stated. She urged the sector not to wait for regulation, arguing that "he legal profession must lead here... through contractual flexibility."

A Unified Strategic Response Bridging the gap between these legal voids and operational burdens, the OceanOpt leadership team - Anil Jacob (Managing Director), Alex Joseph (Consulting Lead), Capt. Mohit Sabharwal (Business Development Lead), and Adon Jacob (Business Analyst) presented their "Compliance to Transformation" framework.

The model posits that successful decarbonization requires a three-stage evolution: securing a compliance baseline, driving performance enhancement through analytics, and finally achieving long-term transformation.

Launching VECTOR This strategic approach underpins VECTOR, OceanOpt’s newly launched emissions intelligence platform. Directly addressing the crew welfare crisis raised by Capt. Ausan, Managing Director Anil Jacob showcased the platform's impact.

"Unified reporting platforms can reduce crew burden dramatically," Jacob said. "Through API integration with partner operators, we’ve achieved single platform reporting on over 100 vessels, reducing time from 90 minutes to 20 minutes daily. This is not just efficiency - it’s operational sustainability."

Consensus: Strategy Beats Compliance The dialogue concluded with a clear consensus: the window for early-mover advantage is closing. "The question facing operators isn’t ‘How do I comply?’ It’s ‘How do I use compliance strategy to compete?" Jacob noted. "The companies moving decisively... will enter 2026 with an advantage."

The products and services herein described in this press release are not endorsed by The Maritime Executive.


Mariners for America Conference

LOOK D.E.I.

Mariners for America & Maritime Institute of Technology and Graduate Studies
Grow, Develop, Sustain

Published Jan 20, 2026 7:57 PM by The Maritime Executive


[By: Maritime Institute of Technology and Graduate Studies]

Help Shape the Future of the U.S. Maritime Workforce
The U.S. maritime industry is preparing for a period of significant growth, and the need for a strong, sustainable American mariner workforce has never been greater. The Mariners for America Conference at MITAGS will bring together vessel operators, unions, academies, training institutions, regulators, and industry leaders to explore bold ideas and practical solutions for attracting, developing, and retaining mariners. 

Download Agenda Here

Why Attend:

  • Stay ahead of maritime industry trends and workforce changes.
  • Gain insights that can elevate your career and organization.
  • Be part of a growing national maritime movement.Help strengthen America’s maritime future.
  • Build meaningful connections with leaders across the U.S. maritime sector.

Registration Is Open:

Early Bird Price:  $900 (Dec 1 – Feb 28)
Standard Price:  $1,000 (after Feb 28)

Register here.

Call for Sponsors

  • Support the mission to grow, develop, and sustain the U.S. mariner workforce by becoming a conference sponsor. 
  • Sponsorship gives your organization visibility among key maritime stakeholders, including vessel operators, maritime schools, regulators, and industry leaders. 
  • Your support will help us deliver a high-quality conference while positioning your brand as a leader in the maritime industry. 
  • To learn more about sponsorship opportunities, please contact us at Events@mitags.org.

Sponsorship Levels:

Pilot Sponsor – $4,000 
Includes signage at registration, logo in the event program, free exhibit table, and recognition in all pre-conference communications. More information here.

Master Sponsor – $2,000
Includes signage at registration, logo in the event program, free exhibit table, and recognition in all pre-conference communications. More information here.

Navigator Sponsor – $1,000
Includes signage at registration, logo in the event program, free exhibit table, and recognition in all pre-conference communications. More information here.

Additional Opportunities

Lanyard Sponsor: $500
Sponsor is responsible for producing and shipping lanyards to the conference venue. More information here.

Exhibit Table: $500
Nonprofits and government organizations — please contact Events@mitags.org to request a table. More information here.

Reception Sponsor: $2,000 (SOLD OUT)
Includes signage at the reception.
Sponsorship at $2,000 would include credit as a Master-Level Sponsor. 

Dinner Sponsor: $2,000
Includes logo placement on all dinner tables.
Sponsorship at $2,000 would include credit as a Master-Level Sponsor. More information here.

Stay On Campus for the Mariners for America Conference

Make the most of your conference experience by staying right on-site at the Maritime Conference Center (MCC) — home of MITAGS and the Mariners for America Conference. Staying on campus keeps you close to every keynote, workshop, and networking opportunity while enjoying the comfort and convenience of being on-site. More information here

The products and services herein described in this press release are not endorsed by The Maritime Executive.

Tuesday, January 20, 2026

 

Export concentration leaves Canada's canola sector vulnerable, research finds amid trade talks






University of Calgary





Calgary, AB – January 19, 2026 

As Canada and China announce a landmark tariff agreement, new research from The Simpson Centre shows that Canada’s canola sector remains structurally vulnerable due to heavy export concentration and limited diversification capacity. 

Based on analysis of Canadian export data from 2010-2025 and examination of market concentration patterns across canola seed, oil, and meal, our research team examined how export dependence on China and the United States creates distinct vulnerabilities across different segments of the canola value chain. These two markets collectively absorb nearly 90% of Canadian canola exports, with limited capacity to reallocate shipments to alternative destinations when trade disruptions occur. 

The research identified several critical challenges preventing export diversification: 

  • Insufficient crushing infrastructure in alternative markets means many countries cannot process Canadian canola seed at scale. 

  • EU restrictions on GMO products effectively close off a major consumer market for Canadian canola oil. 

  • Domestic transportation bottlenecks limit Canada’s ability to handle increased volumes of processed oil even as crushing capacity expands.  

Addressing these issues is essential for building resilience in Canada’s export-dependent agricultural sectors. 

“While the prime minister’s latest deal with China, which may drop seed duties nearly 70% by March, is welcome news, the fact remains: even as one barrier comes down, the underlying structural vulnerabilities persist,” said Farzana Shirin, Agricultural and Applied Economist, The Simpson Centre.  

The research comes at a pivotal time for Canada’s canola sector, as negotiators work to restore normal trade relations with China while uncertainty persists around U.S. biofuel policy changes that could exclude Canadian feedstocks from critical incentive programs. With Canadian producers managing simultaneous challenges in both major export markets, understanding the constraints preventing rapid diversification is essential for developing effective policy responses that strengthen industry resilience beyond short-term tariff relief. 

This research exemplifies The Simpson Centre’s mandate to bridge cutting-edge analysis and practical policy outcomes. The findings contribute to informed decision-making at all levels of government and inform policy frameworks addressing export concentration risks, domestic processing investment, and trade diversification strategies for agricultural commodities. 

Wednesday, January 14, 2026

 

ABB Chosen to Supply Technology for BC Ferries’ New Major Vessels

ABB Marine & Ports
ABB’s integrated power, propulsion and control solution has been chosen for BC Ferries’ four new hybrid-electric major vessels. The New Major Vessels have been designed to minimize emissions and underwater radiated noise (URN), with the goal of contributi

Published Jan 13, 2026 6:19 PM by The Maritime Executive


[By: ABB]

ABB will supply a complete package of power, propulsion and control technology for four new double-ended passenger and car ferries operated by British Columbia Ferry Services (BC Ferries). One of the largest ferry operators in the world, BC Ferries provides year-round vehicle and passenger service on 25 routes to 47 terminals, carrying approximately 9.7 million vehicles and 22.7 million passengers annually. Demand on the ferry system is expected to increase as the province’s population is forecast to grow 44 percent by 2046.

The hybrid-electric ferries, which will replace four end-of-life vessels, are part of the BC Ferries’ New Major Vesselsprogram, aimed at delivering safe, environmentally sustainable and reliable operations in and around the Strait of Georgia, the body of water separating Vancouver Island from the Lower Mainland of British Columbia. The order was booked in the fourth quarter of 2025.

Scheduled for delivery beginning in 2029 from China Merchants Industry Weihai (CMI Weihai) Shipyard, the vessels will be equipped with ABB’s gearless, steerable Azipod® electric propulsion.The system offers proven reliability thanks to significantly fewer moving parts than mechanical thrusters, while the special propeller design helps reduce underwater radiated noise (URN). This helps safeguard at-risk species, such as the Southern Resident killer whale, and preserve one of the world’s most biologically rich marine ecosystems3.

ABB’s Onboard DC Grid™ power distribution system will serve as the backbone for efficient energy flow, minimizing conversion losses and enabling higher overall system efficiency and lower emissions than comparable propulsion arrangements.

Each ferry will be equipped to accommodate up to 70 megawatt-hours (MWh) of battery energy storage. This enables efficient hybrid operations today and supports a future shift to fully electric, zero-emission service. The hybrid configuration uses biofuel or renewable diesel and continuously balances energy between generators and batteries. Each vessel can also connect to a high-capacity shore charging system rated above 60 megawatts (MW) for full electric operation. This system is more than 100 times more powerful than the fastest public electric vehicle charging stations in North America, which typically deliver up to 500 kilowatts (kW) per plug. This high-capacity charging supports fast turnaround in port and enables the transition to zero-emission operations.

ABB’s digital solutions will give crews a clear overview of ship operations and support safe, efficient journeys. These digital technologies are intended to help BC Ferries deliver an improved travel experience for passengers while reducing environmental impact.

“BC Ferries’ New Major Vessels represent the largest capital investment in our history and are essential to renewing our fleet, increasing capacity on our busiest routes, and strengthening system resilience,” said Nicolas Jimenez, President & CEO, BC Ferries. “Their design reflects what our customers value most: comfort, accessibility and environmental stewardship. With diesel-battery hybrid technology that can operate on bio and renewable diesel today and transition to full electrification as infrastructure evolves, these ships are a critical part of building a cleaner, quieter, and more reliable ferry system for the future."

“We proudly support BC Ferries’ goals to reduce greenhouse gas emissions from their operations, striving to meet British Columbia’s 2030 greenhouse gas emissions reduction target for the transportation sector4 by at least 27 percent by 2030, from 2008 levels, in support of a cleaner future for British Columbia, and its ambitions to transition to all-electric operation,” said Rune Braastad, President, ABB’s Marine & Ports division. “ABB’s deep roots in Canada make it possible to support generational infrastructure projects like the New Major Vessels.”

“Winning the contract to deliver such a wide scope of solutions is highly significant for ABB’s marine business in North America,” said Timo Vesala, Head of Sales, Marine Systems, Americas, ABB’s Marine & Ports division. “As someone who lives and works in Vancouver, I recognize the importance of this initiative for British Columbia – not only in providing consistently reliable and resilient ferry services, but also in helping local communities experience cleaner air and quieter waterways.”

The products and services herein described in this press release are not endorsed by The Maritime Executive.

Monday, January 12, 2026

 

Gamma rays quickly toughen nitrogen‑fixing bacteria



QST team pairs experimental evolution with controlled gamma irradiation to create heat‑tolerant biofertilizer strains in weeks, pointing to faster, greener production for food, pharma, and biofuels




The National Institutes for Quantum Science and Technology

Wild-type vs high-temperature-tolerant Bradyrhizobium diazoefficiens mutants 

image: 

Heat-tolerant mutant lines of rhizobia obtained by experimental evolution combined with repeated mutagenesis with gamma rays

view more 

Credit: Dr. Yoshihiro Hase from the National Institutes for Quantum Science and Technology, Japan




Takasaki, Japan — Heat‑resilient biofertilizers could help crops cope with rising temperatures but engineering them has been slow and uncertain. A new study at the National Institutes for Quantum Science and Technology (QST) shows that pairing experimental evolution with controlled gamma‑ray mutagenesis can accelerate the path to heat‑tolerant nitrogen‑fixing bacteria, shortening development timelines and opening practical routes to more reliable, climate‑ready microbial products for agriculture, food processing, pharmaceuticals, and biofuel production. The study was made available online on November 19, 2025, and published in Volume 831 on July, 01, 2025, in the Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis journal.

The team focused on Bradyrhizobium diazoefficiens USDA110, a workhorse bacterium used to help soybean and other legumes capture nitrogen. While the wild-type grows best at around 32–34 °C and stalls at ~36 °C, QST researchers raised culture temperatures stepwise from 34 °C to 37 °C over 76–83 days and irradiated populations ten times at specific doses, then selected the lines that continued to form robust colonies at 36 °C.

A clear “sweet spot” emerged: around 40 Gy produced the greatest number of stable, heat‑tolerant lines, whereas higher doses (80–120 Gy) initially yielded more tolerant lines but with smaller colonies and traits that faded when selection relaxed, consistent with an excess of deleterious mutations. In practical terms, the method lets researchers tune the mutation load to favor beneficial changes while preserving overall fitness.

Genomic analyses of the top performers revealed changes in two core genes across independently evolved lines: the 16S rRNA gene, central to the protein‑making machinery, and rpoC, which encodes the β subunit of RNA polymerase. Convergent mutations in such essential systems point to mechanisms that help bacterial transcription and translation continue smoothly under heat stress—precisely the behaviors industry needs in high‑temperature processes.

By combining adaptive laboratory evolution with precisely repeated doses of gamma rays, we shortened the path to robust, heattolerant bacteria from months or years to just weeks,” said Dr. Yoshihiro Hase, project leader at the Takasaki Institute for Advanced Quantum Science (TIAQ), QST. “It’s a practical lever for making biofertilizers more reliable in hotter fields and bioreactors.”

This controllable mutagenesis avoids transgenic modifications and can be tuned to maximize beneficial changes while limiting genetic load,” added Dr. Katsuya Satoh, senior principal researcher at TIAQ. “We see a route that industry can adopt safely to boost resilience and productivity.”

Beyond agriculture, the approach could be generalized to yeasts, bacteria, and microalgae used in food processing, therapeutic manufacturing, and biofuel production—helping deliver high‑quality products at lower environmental cost. In the long term, QST anticipates ultra‑low‑cost microalgal cultivation and other heat‑tolerant platforms that contribute to food and energy security.

 

***

 

Reference
DOI: 10.1016/j.mrfmmm.2025.111919

 

About National Institutes for Quantum Science and Technology, Japan
The National Institutes for Quantum Science and Technology (QST) was established in April 2016 to promote quantum science and technology in a comprehensive and integrated manner. The new organization was formed from the merger of the National Institute of Radiological Sciences (NIRS) with certain operations that were previously undertaken by the Japan Atomic Energy Agency (JAEA).

QST is committed to advancing quantum science and technology, creating world-leading research and development platforms, and exploring new fields, thereby achieving significant academic, social, and economic impacts.

Website: https://www.qst.go.jp/site/qst-english/

 

About Dr. Yoshihiro Hase
Dr. Yoshihiro Hase works at the Takasaki Institute for Advanced Quantum Science, National Institutes for Quantum Science and Technology, Japan. His research focuses on mutagenesis by quantum beams in plants and bacteria and he has published more than 70 papers on these topics, which have received more than 1,600 citations.

 

Funding information
This study was partially supported by The Canon Foundation.

Monday, January 05, 2026

 

Herbarium records lead Bucknell researcher to a new plant species in the Australian outback



Specialized organs for feeding ants are first of their kind



Pensoft Publishers

Morphology of Solanum nectarifolium 

image: 

Morphology of Solanum nectarifolium, a newly-described species of Australian bush tomato.

view more 

Credit: Kym Brennan





LEWISBURG, Pa. — A recent study led by Bucknell University Professor Chris Martinebiology, the David Burpee Professor in Plant Genetics & Research, has identified and described a new species of bush tomato with a special connection to ants — a taxonomic journey sparked by unusual specimens held in Australian herbarium collections.

The study, co-authored by a set of Australian botanists and Jason Cantley — the former Burpee Postdoctoral Fellow in Botany at Bucknell who is now Associate Professor of Biology at San Francisco State University — was published in the open-access journal PhytoKeys and underscores the critical role that natural history collections play in biodiversity science. The new species, Solanum nectarifolium, or the Tanami Bush Tomato, was named for the location of its original collection area — the northern edge of the Tanami Desert — and for the uniquely conspicuous nectar-producing organs on the undersides of its leaves. These extrafloral nectaries exude a sweet liquid to attract ants that might protect the plant from herbivores. This remarkable trait marks the first known Solanum species with extrafloral nectaries visible to the naked eye, a feature previously observed only microscopically in a handful of related Australian species.

Martine first had an inkling that something was unusual about the plants from that region of the Northern Territory while working on a project with another former Burpee Postdoc, Angela McDonnell, now an Assistant Professor at St. Cloud State University. The pair included DNA extracted from two herbarium specimens representing Solanum ossicruentum, a species known as the Blood Bone Tomato that the Martine Lab described in the same journal in 2016, in an ongoing analysis meant to build a new bush tomato evolutionary tree.

“We couldn’t understand why the two collections of the same species kept showing up in different parts of the tree,” says Martine. “I had collected one of them and was certain that it represented Solanum ossicruentum, so I reached out to the person who collected the other one, David Albrecht, and asked whether he thought the plants he saw in 1996 at a place called Jellabra Rockhole could be something else.”

Albrecht, Senior Botanist at the Northern Territory Herbarium at Alice Springs, suggested that the best way to know would be for botanists to revisit that remote region of the northwestern Tanami Desert and see for themselves. Martine, who had participated in seven collecting expeditions to northern Australia since 2004, wasn’t disappointed.

“I was kind of hoping he’d tell me that,” Martine says. “Because I was already planning some new fieldwork in the Northern Territory and this would give me a great season to visit an area I had never been to before. But to really be prepared for a trip like that, I first needed to understand what other botanists had recorded and collected there in the past – and there is only one surefire way to do that: check what is in the herbarium collections.”

So Martine started by using the Australasian Virtual Herbarium (AVH), a database of every plant specimen held in every herbarium in Australia. He searched for collections made of Solanum ossicruentum and a similar species called Solanum dioicum in the northern Tanami, finding 15 records for specimens gathered as far back as 1971.

“It was a really interesting distribution of points on the map, too,” Martine says. “These were far south of the other records for Solanum ossicruentum, with hundreds of miles of ‘empty’ country between the two clusters. I couldn’t wait to get to Australia to see what those Tanami plants looked like.”

In May 2025 Martine headed to Australia to meet his team for the trip: Cantley and paper coauthors Kym Brennan, Aiden Webb, and Geoff Newton, all associated with the Northern Territory Herbarium at Palmerston. But, first, Martine made a stop in another plant collection in the southwestern city of Perth.

“The visit to the Western Australian Herbarium was my first chance to spend a bunch of time with some of the actual specimens that I had earmarked based on the data in AVH,” Martine explains. “And what I saw there legit blew my mind.”

Every specimen looked similar to Solanum ossicruentum, except for a few subtle characteristics – and one thing that Martine had never seen in more than two decades of Outback botanizing.

“On the backs of the leaves, along the veins, were these visible round disks,” Martine notes. “They were each around a half-millimeter wide, really obvious, and the only bush tomato specimens that had them – we’re talking hundreds and hundreds of collections – were the ones from the northern Tanami.”

Martine thought they could be extrafloral nectaries (EFNs), non-flower organs on a plant that exude sweet liquid, typically as a means to attract ants that might protect the plants from herbivores. These were known to exist in a few Australian bush tomatoes, but those are tiny and have only been confirmed with microscopes. EFNs that could be seen without magnification would be something truly novel.

A few days later, Martine was in the herbarium at Palmerston and found the same pattern: more visible disks and only on plants from that same geographic area. Then he noticed that the most recent collection, from 2021, had been made by Kym Brennan – a renowned field biologist with an expertise in photography who was preparing for their trip in the next room.

“I ran in there and asked whether he remembered anything unusual about that collection – and before I could finish my explanation for why, he was already showing me an incredible photo of the leaves of that same plant. They were positively oozing with shiny, round droplets of nectar. And all from those disks on the veins.” 

Eight days and more than 1000 kilometers of driving later the team arrived near Brennan’s collection site 50 kilometers southwest of the community of Lajamanu, right along the edge of the unpaved Lajamanu Road.

“This was more-or-less the same place where others had collected it in the early 1970s, so we were cautiously optimistic that we’d not only find it there again, but that the plants would have the flowers and fruits on them that we needed to describe this as a new species,” explains Martine. “But it’s a harsh environment and the abundance of bush tomatoes is often dependent on fire occurrence. Sometimes you get to a place and there is nothing but old gray stems. Other times there are more happy plants than you can count. In this case, it was the latter situation!”

The team got to work taking notes, making measurements, and shooting photographs. And then Cantley called for Martine to come over to the plant he was examining. There were ants all over the leaf undersides, avidly moving from disk to disk and probing them for nectar. Hypothesis confirmed.

The collaborators decided on the scientific name “nectarifolium” – which translates to “nectar leaf,” for obvious reasons – and the English-language name Tanami Bush Tomato. Martine then contacted a few experts about the conspicuous nature of the EFNs and whether that has been seen anywhere else in the genus Solanum, a group of around 1200 species that includes the tomato, potato, and eggplant.

“As far as we know, this is the first Solanum species to be described as having extrafloral nectaries that you can see with your naked eye. That’s a pretty cool finding – and it all started with the examination of specimens that have been waiting in herbaria for as long as a half-century for someone to come along and take a closer look.”

Bucknell’s own Wayne E. Manning Herbarium, which holds approximately 25,000 plant specimens, now includes new samples of the Tanami Bush Tomato. But the official holotype remains at the Northern Territory Herbarium in Palmerston — almost 10,000 miles away from Bucknell’s campus.

“The Manning Herbarium may be small, but every specimen is a snapshot of biodiversity,” Martine says. “These collections allow us to study where species occur, how they’ve changed over time, and — in cases like this — even help discover new ones.”

The publication of the new species comes amid broader concern over the fate of natural history collections, such as Duke University’s recently announced closure of its herbarium housing more than 800,000 specimens. Martine and his colleagues agree that such closures could hinder future discoveries and conservation efforts.

Martine, a leading expert on Australian bush tomatoes, was recently elected president-elect of the Botanical Society of America. He will begin his term as president following the organization’s annual meeting in August 2026.

“It still doesn’t feel real and probably won’t until I start my term just after Botany 2026,” Martine says. “But I promise to do my best because plants are awesome and so are botanists.”

Original study:

Martine, C.T., Brennan, K., Cantley, J.T., Webb, A.T. and Newton, G. (2025). A new dioecious bush tomato, Solanum nectarifolium (Solanaceae), from the northern Tanami Desert, Northern Territory, Australia, with reassessment of S. ossicruentum and a change in the circumscription of S. dioicumPhytoKeys, 268, pp.183–199. doi: https://doi.org/10.3897/phytokeys.268.169893

  

Immature fruit and fruiting calyx of Solanum nectarifolium, a newly-described species of Australian bush tomato.  

Extrafloral nectaries (EFNs) on the leaves of Solanum nectarifolium.

Staminate flowers of Solanum nectarifolium, a newly-described species of Australian bush tomato.

Credit

Kym Brennan

New "Stomata in-sight" system allows scientists to watch plants breathe in real-time




University of Illinois at Urbana-Champaign Institute for Sustainability, Energy, and Environment

Representative 16-bit confocal microscope image of an open Zea mays stoma. 

image: 

Representative 16-bit confocal microscope image of an open Zea mays stoma.

view more 

Credit: Plant Physiology, Volume 199, Issue 4, December 2025, kiaf600, https://doi.org/10.1093/plphys/kiaf600





URBANA, Ill. — For centuries, scientists have known that plants "breathe" through microscopic pores on their leaves called stomata. These tiny valves are the gatekeepers that balance the intake of carbon dioxide into the leaf for photosynthesis against the loss of water vapor from the leaf to the atmosphere. Now, researchers at the University of Illinois Urbana-Champaign have developed a groundbreaking new tool that allows them to watch and quantify this process in real-time and under strictly controlled environmental conditions.
The study, published in the journal Plant Physiology, introduces a system dubbed "Stomata In-Sight." It solves a long-standing technical challenge in plant biology: how to observe the microscopic movements of stomatal pores while simultaneously measuring how much gas they are exchanging with the atmosphere.
The "Mouths" of the Plant, stomata (Greek for "mouths"), are critical to global agriculture. When they open, plants get the carbon they need to grow, but they also lose water. Therefore, understanding how the number and operation of these pores determine the efficiency of photosynthetic gas exchange is key to developing crops that need less water to grow and can reliably produce food, biofuel and bioproducts in times and places of drought stress.
"Traditionally, we've had to choose between seeing the stomata or measuring their function," explained the research team. Previous methods often involved making impressions of leaves (like taking a dental mold), which only captures a static snapshot, or using standard microscopes that observe the leaf without being able to control the conditions the leaf is experiencing. This is important because the stomata are highly responsive to variation in almost all aspects of the environment.
A Window into the Leaf The new "Stomata In-Sight" system integrates three complex technologies into one:
1.    Live Confocal Microscopy: A powerful imaging technique that uses lasers to create detailed, three-dimensional views of living cells without slicing into the plant.
2.    Leaf Gas Exchange: High-precision sensors that measure exactly how much CO2 the leaf is taking in and how much water it is releasing.
3.    Environmental Control: A chamber that allows researchers to manipulate light, temperature, humidity, and carbon dioxide levels to mimic real-world conditions.
By combining these, the team can watch exactly how the stomata respond to variation in the environment.
Why It Matters This high-definition view of plant physiology could revolutionize how we breed crops. By understanding the precise mechanical and chemical signals that cause stomata to open or close, and how that is influenced by the number of stomata on a leaf, scientists can identify genetic traits that lead to "smarter" plants—crops that use water most efficiently. That is crucial because water is the environmental factor that limits agricultural production the most.
The system was developed by Joseph D. Crawford, Dustin Mayfield-Jones, Glenn A. Fried, Nicolas Hernandez, and Andrew D.B. Leakey at the Department of Plant Biology and the Institute for Genomic Biology at the University of Illinois.
About the Paper The work was supported by the U.S. Department of Energy's Center for Advanced Bioenergy and Bioproducts Innovation, the National Science Foundation, and a philanthropic gift, and is published as an open-access article titled, "Stomata In-Sight: Integrating Live Confocal Microscopy with Leaf Gas Exchange and Environmental Control," in Plant Physiology. https://doi.org/10.1093/plphys/kiaf600
Contact: Andrew Leakey, leakey@illlinois.edu