Monday, November 01, 2021

Vanderbilt astrophysicist leads international workshop to turn plans for a crewed lunar observatory into reality


Meeting Announcement

VANDERBILT UNIVERSITY

Karan Jani, research assistant professor of physics and astronomy, co-chaired the first international workshop focused on gravitational wave detection on the moon. The workshop builds on Jani’s recent studies that make the case for building a crewed, lunar-based observatory. 

“We are at the dawn of a new space age, with the moon at the center of our campaign for the next several years,” Jani said. “This workshop drew a broad consensus that fundamental physics and astronomy have immense potential on the moon.” 

The workshop convened more than 350 leading experts from the fields of GW science, planetary science and lunar exploration to discuss the geophysical properties of the moon and opportunities for this type of observation with key technologies that are already at an advanced stage of development. Participants were in agreement that lunar GW detection could become a realistic space science mission with international cooperation.  

Opportunities for breakthrough science exist through partnerships with other future detectors, like the joint European Space Agency and NASA space mission Laser Interferometer Space Antenna, and with proposed terrestrial GW detectors the Einstein Telescope and the Cosmic Explorer. Discussions highlighted the exciting possibility that a GW detector on the moon could provide a more complete understanding of where gravitational waves come from, and the orbit of the moon around Earth would significantly increase the ability to triangulate the position of those sources.  

“The lunar GW detectors can probe the most important questions about our universe, from the nature of dark energy to the birth of the first stars,” Jani said. “Many of the required technologies are already being developed by space agencies and major private companies. It’s only a matter of time before we have our graduate students taking shifts on the moon!”  

The workshop was well attended, reflecting worldwide interest in GW science and lunar exploration, said Jan Harms, professor at the Gran Sasso Science Institute. “We now need to work hard to seize the opportunities given to us to realize a first lunar GW detector. Turning our moon into a resonant antenna for messages from the universe would be a historical achievement.” 

And officials at high-level funding agencies were attentive, added Stavros Katsanevas, European Gravitational Observatory director, which is “a very good omen for the development of this emerging field. 

“We were all impressed by the quality of the presentations, key elements of which were the interdisciplinarity between GW science and geoscience, the complementarity of the proposed instrumentation guaranteeing the detailed understanding of lunar vibrations.”  

The workshop took place on Oct. 14 and 15 and was hosted by the European Gravitational Observatory near Pisa, Italy. Jani was joined by researchers from EGO and Gran Sasso Science Institute. The workshop was supported by the AHEAD 2020 project, funded from the European Union’s Horizon 2020 research and innovation program under grant agreement 871158. 

Urgent changes needed to global guidelines designed to stop surgical infection


Peer-Reviewed Publication

UNIVERSITY OF BIRMINGHAM

Wound infections are the most common problem after surgery, particularly in developing countries, but promised innovations to tackle the issue do not work and global guidance needs changing, a new study reveals.

Both World Health Organisation (WHO) and the UK’s National Institute of Health Research guidelines recommend that surgeons use alcoholic chlorhexidine skin preparation and triclosan coated sutures to prevent Surgical Site Infection (SSI).

However, the world’s largest wound infection trial could not demonstrate superiority of these interventions over lower cost alternatives.

Carried out in Benin, Ghana, India, Mexico, Nigeria, Rwanda and South Africa, the FALCON trial was funded by the UK’s National Institute for Health Research (NIHR).

Publishing their findings today in The Lancet, researchers participating in this study are calling for guidelines recommending these measures, either specifically to Low- and Middle-income Countries (LMIC) or at a general global level, to be revised.

Co-author Mr. Aneel Bhangu, from the University of Birmingham, commented: “Surgical site infection is the world’s most common postoperative complication - a major burden for both patients and health systems. We have delivered the biggest trial of its kind, where we could not demonstrate the superiority of these interventions over cheaper alternatives.

“Our findings are hugely important for a wide range of care providers in LMICs, as following existing WHO and NICE guidelines, which have significant cost implications for organisations which have limited resources.”

Patients who develop SSI experience pain, disability, poor healing with risk of wound breakdown, prolonged recovery times and psychological challenges.

Those patients in LMICs are disproportionately affected by higher rates of SSI compared to those in high-income countries - increasing the risk of catastrophic expenditure, impoverishment, and wider negative community impact.

The NIHR Global Research Health Unit on Global Surgery trial covered 5,788 patients from 54 hospitals in seven countries - a broad and representative range including adults and children undergoing contaminated/dirty surgery, emergency surgery and caesarian section.

Co-author Professor Adesoji Ademuyiwa, from the University of Lagos, commented: The overall SSI rate was very high at 22% - a preventable complication that is causing unnecessary suffering and burden to patients and systems.

“It is clear that small randomised trials should now be avoided and should be replaced with larger trials that can provide more robust evidence on the incidence of SSI, ultimately leading to more effective measures to help tack this global healthcare challenge.”

For further information, interviews or to request an embargoed copy of the research paper please contact Tony Moran, International Communications Manager, University of Birmingham on +44 (0)782 783 2312 or t.moran@bham.ac.uk. Out-of-hours, please call +44 (0) 7789 921 165.

Notes for editors

  • The University of Birmingham is ranked amongst the world’s top 100 institutions. Its work brings people from across the world to Birmingham, including researchers, teachers and more than 6,500 international students from over 150 countries.
  • ‘Reducing surgical site infections in low and middle income countries: a pragmatic, multicentre, stratified, randomised controlled trial (FALCON)’ - Adesoji O Ademuyiwa and Aneel Bhangu is published in The Lancet.
  • Please feel free to include this post-embargo link in online articles: https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(21)01548-8/fulltext

The mission of the National Institute for Health Research (NIHR) is to improve the health and wealth of the nation through research. We do this by:

  • Funding high quality, timely research that benefits the NHS, public health and social care;
  • Investing in world-class expertise, facilities and a skilled delivery workforce to translate discoveries into improved treatments and services;
  • Partnering with patients, service users, carers and communities, improving the relevance, quality and impact of our research;
  • Attracting, training and supporting the best researchers to tackle complex health and social care challenges;
  • Collaborating with other public funders, charities and industry to help shape a cohesive and globally competitive research system;
  • Funding applied global health research and training to meet the needs of the poorest people in low and middle income countries.

NIHR is funded by the Department of Health and Social Care. Its work in low and middle income countries is principally funded through UK Aid from the UK government.

What big teeth you have: Tooth root surface area can determine primate size


Peer-Reviewed Publication

NORTH CAROLINA STATE UNIVERSITY

Gorilla and Lemur Skuls 

IMAGE: THE SKULLS OF A GORILLA AND LEMUR WITH THE TEETH "DIGITALLY DISSECTED" IN ORDER TO STUDY THEIR TOOTH ROOT SURFACE AREAS. view more 

CREDIT: CREDIT: DEUTSCH AND HARTSTONE-ROSE.

An often overlooked feature could give scientists new insight into the lives of ancient primate species. Researchers from North Carolina State University have developed formulas that can calculate the body size of a primate based on the root size of its teeth. The formulas could allow researchers to make use of partial and incomplete fossils in order to learn how ancient primates – including human ancestors – interacted with their environment.

Ashley Deutsch, NC State graduate student and first author of a paper describing the research, wanted to know if it was possible to determine what a primate’s diet was without having the actual tooth crowns at hand – by looking instead at the roots.

What we commonly think of when we think of teeth isn’t the whole story – the part that does the chewing is merely the crown. The part that keeps the tooth anchored in the jaw is the root.

“The tooth root transmits the force of the jaws into the food,” says Adam Hartstone-Rose, professor of biological sciences at NC State and paper coauthor. “You can think of the root as the handle of a hammer – the handle size is related to the amount of force you can put into the hammer. So if a hammer has a small handle, it will have a small head to hammer small things. In the same way, a big tooth root can transmit more force to the tooth’s crown to crush more obdurate foods.”

Deutsch and the research team initially set out to determine whether tooth roots could indicate the shape of the tooth’s crown, thus telling them what particular primates preferred to eat. Using computer tomography, Deutsch analyzed and calculated the tooth root surface area – or area of contact where the root fits into the jaw – of 70 primates from 75 species, ranging in size from tiny mouse lemurs to great apes.

“It was a bit like trying to figure out if you have an axe or hammer based on the shape of the handle,” Deutsch says.

Ultimately, she found that the tooth roots only related to diet in a few lineages (for example, lemurs); however, she was able to determine how big the primates were across all lineages.

Deutsch developed a series of formulas based on the relationship between the tooth root surface of a molar or premolar – the teeth located between canines and molars – and primate body mass. The formulas can be used to estimate body mass of primates with more or less specificity, depending upon whether their class is known. The formulas can also explain up to 96% of variation in body mass within the examined primate sample.

But perhaps the most useful application of the equation will be with fossils that are currently of little use to anthropologists and paleontologists.

“As long as the fossil has a bit of root you can use the formulas even if the tooth crowns are missing,” Deutsch says. “Fossils are often maddeningly incomplete, but now those incomplete pieces can be useful, and they could answer questions about our own lineage, like how big our ancient ancestors were.”

Deutsch hopes to continue refining the existing equations and expand the work beyond primates to other mammals, including carnivores.

“Ashley has looked at something that is often preserved but also generally ignored by science and found it might hold answers to one of the most important ecological questions,” Hartstone-Rose says. “Just knowing how big an animal is tells you a lot about how it interacted with the environment. And that could give us a lot of insight into our own ancient history.”

The research appears as the cover article in the American Journal of Biological Anthropology and is supported by the National Science Foundation (grant IOS-15-57125). Former NC State postdoctoral fellow Edwin Dickinson, as well as NC State undergraduates Victoria Whichard and Giulia Lagomarsino, also contributed to the work.

-peake-

Note to editors: An abstract follows.

“Primate Body Mass and Dietary Correlates of Tooth Root Surface Area”

DOI: 10.1002/ajpa.24430

Authors: Ashley R. Deutsch, Edwin Dickinson, Victoria A. Whichard, Giulia R. Lagomarsino, Adam Hartstone-Rose, North Carolina State University; Jonathan M. G. Perry, Western Universty of Health Sciences, Oregon; Kornelius Kupczik, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
Published: October 26, 2021 in the American Journal of Biological Anthropology

Abstract:
Objectives: This study aims to examine primate postcanine tooth root surface area (TRSA) in the context of two ecological variables (diet and bite force). We also assess scaling relationships within distinct taxonomic groups and across the order as a whole.
Materials and Methods: Mandibular postcanine TRSA was measured using a three-dimensional computed tomography (CT) method for catarrhine (N = 27), platyrrhine (N = 21) and strepsirrhine (N = 24) taxa; this represents the first sample of strepsirrhines. Two different body size proxies were used: cranial geometric mean (GM) using nine linear measurements, and literature-derived body mass (BM).
Results: TRSA correlated strongly with body size, scaling with positive allometry or isometry across the order as a whole; however, scaling differed significantly between taxa for some teeth. Among Strepsirrhini, molar TRSA relative to GM differed significantly between folivores and pliant-object feeders. Additionally, P4 TRSA relative to BM differentiated folivores from both hard- and pliant-object feeders. Among Cercopithecoidea, P4 TRSA adjusted by GM differed between hard- and pliant-object feeders.
Discussion: Dietary signals in TRSA appear primarily driven by high frequency loading experienced by folivores. Stronger and more frequent dietary signals were observed within Strepsirrhini relative to Haplorhini. This may reflect the constraints of orthognathism within the latter, constraining the adaptability of their postcanine teeth. Finally, because of the strong correlation between TRSA and BM for each tooth locus (mean r2 = 0.82), TRSA can be used to predict BM in fossil primates using provided equations.

New public health education programs to address workforce needs


Federal initiative awards $3 million to UMass Lowell

Grant and Award Announcement

UNIVERSITY OF MASSACHUSETTS LOWELL

LOWELL, Mass. – UMass Lowell has been awarded a $3 million cooperative agreement from the U.S. Department of Health and Human Services’ Office of the National Coordinator for Health Information Technology to develop new undergraduate and graduate programs in public health informatics and technology, to educate diverse students for vital jobs.

The grant, one of 10 awarded to minority-serving institutions and other institutions of higher education around the country, is part of the Public Health Informatics & Technology Workforce Development Program, a federal effort to address the gaps in public health capacity that hurt the U.S. response to COVID-19. The funding comes from the American Rescue Plan, the $1.9 trillion federal aid package designed to address the public health and economic impacts of the pandemic.

“Tremendous weaknesses in our public health data infrastructure were exposed by the COVID-19 pandemic,” said UMass Lowell Public Health Prof. Dan Berlowitz, chair of the department, who is leading the initiative at the university. “Information systems were not there, and data-handling expertise and management were not there for tracking positive tests, contacting people who had been exposed, identifying high-risk populations, and reporting illness and death. And even when they were there, everyone was doing it differently.”

The project will include several other UMass Lowell public health professors, faculty from the Solomont School of Nursing, the Department of Computer Science and the Manning School of Business, allowing the university to develop a new undergraduate public health pathway in health informatics and technology over the next year, as well as a new graduate program and graduate certificates for people already in the workforce.

As part of the federal effort, UMass Lowell will also aim to increase the number of people from underrepresented communities in the public health IT workforce and to educate all students in health equity. 

UMass Lowell is partnering with Middlesex and Northern Essex community colleges as it develops the new undergraduate major to make sure students who graduate from the two-year schools can transfer smoothly to UMass Lowell. All three schools are designated as “minority-serving institutions” by the U.S. Department of Education, Berlowitz said. 

UMass Lowell also will work with community partners throughout the Merrimack Valley to place students in internships and make sure the new programs meet local job needs.

“We could not have received this grant without our educational partners and our strong relationships with community partners,” Berlowitz said.

The new concentration for undergraduate public health majors will combine classes in computer science and public health, as well as business classes, according to UMass Lowell’s Amy Smalarz, assistant teaching professor and undergraduate program coordinator for public health.

“Teaching students how to build those databases, how to organize them and how to use them is a big piece,” she said.

The new graduate degree and certificates offered through UMass Lowell’s Division of Graduate, Online and Professional Studies will draw on courses for the existing master’s degree in health information management with a focus on health informatics.

The community and industry partners on the grant include the city of Lowell Health Department, the city of Lawrence Board of Health, the Greater Lowell Health Alliance, the Greater Lawrence Family Health Center, Lowell General Hospital, the Department of Veterans Affairs Center for Healthcare Organization and Implementation Research, the UMass Chan Medical School Division of Health Informatics and Implementation Science, LLX Solutions, and Academic Public Health Corp.

UMass Lowell is a national research university located on a high-energy campus in the heart of a global community. The university offers its students bachelor’s, master’s and doctoral degrees in business, education, engineering, fine arts, health, humanities, sciences and social sciences. UMass Lowell delivers high-quality educational programs, vigorous hands-on learning and personal attention from leading faculty and staff, all of which prepare graduates to be leaders in their communities and around the globe. www.uml.edu

Oral hookworm vaccine could save millions around the world


Peer-Reviewed Publication

UNIVERSITY OF QUEENSLAND

Hookworm in intestine 

IMAGE: AN ARTIST'S IMPRESSION OF HOOKWORMS INSIDE A HUMAN INTESTINE. view more 

CREDIT: THE UNIVERSITY OF QUEENSLAND

There’s been a significant breakthrough in the development of a vaccine to prevent hookworm infection – a parasite which causes serious disease in tens of millions of people globally.

Trials of the vaccine candidate in mice, led by researchers at The University of Queensland, indicate that it is more than twice as effective than existing alternatives and marks a leap forward in the battle against the highly contagious parasite.

Professor Istvan Toth from UQ’s School of Chemistry and Molecular Biology said the ease with which the vaccine could be administered –  via tablet, liquid or powder – would be a gamechanger for developing countries.

“Our vaccine candidate can be orally self-administered, bypassing the need for trained medical staff, and means there’s no requirement for special storage, enabling it to reach large, isolated populations,” Professor Toth said.

“Vaccination can be carried out at a significantly reduced cost, which not only improves the health of those affected and at high risk, but also helps improve economic growth in disease-endemic areas.”

Hookworm currently infects around half a billion people globally and lives within the human intestine, using the host’s blood as its source of nourishment, digested through a special set of enzymes.

It’s often found in regions with poor water quality, sanitation, and hygiene – greatly impacting on the physical and cognitive development of children and increasing the risk of mortality and miscarriage.

UQ’s trials in mice showed significant improvements on an alternative vaccine candidate which only achieved a 30 to 50 per cent reduction in the number of worms.

“The UQ-developed vaccine resulted in an impressive 94 per cent worm reduction in mice,” Professor Toth said.

“So not only is our new vaccine candidate easier to deliver, it triggers a staggeringly good immune response.”

Co-author of the paper Dr Mariusz Skwarczynski said the research team targeted the hookworm’s digestion enzyme (APR 1).

“When the function of these enzymes is blocked, the parasite starves,” Dr Skwarczynski said.

“Our vaccine produces antibodies against the hookworm enzymes responsible for the digestion of blood – they simply stop being able to eat properly.”

The researchers plan to continue working on and refining the vaccine candidate in preclinical development settings, to ensure its safety and efficacy, before beginning human clinical trials.

“We’re very optimistic that, along with our colleagues, led by Professor Alex Loukas from James Cook University, we will be able to deliver a successful vaccine that stops this parasite in its tracks,” Dr Skwarczynski said.

The research has been published in Scopus (DOI: 10.3390/vaccines9091034).

Psychologists create first-ever body-maps of hallucinations

Leicester psychologists have, for the first time, created body-maps of the sensations which arise during hallucinations in people experiencing psychosis.

Peer-Reviewed Publication

UNIVERSITY OF LEICESTER

Figure_3 

IMAGE: EXAMPLE OF PARTICIPANT ANNOTATIONS, USED TO CREATE NOVEL BUT SIMPLE MULTIMODAL UNUSUAL SENSORY EXPERIENCE (MUSE) BODY-MAPS. view more 

CREDIT: UNIVERSITY OF LEICESTER

Leicester psychologists have, for the first time, created body-maps of the sensations which arise during hallucinations in people experiencing psychosis.

The study, published in The Lancet’s EClinicalMedicine, provides the most extensive descriptive data to date on the feelings which arise during hallucinations and where individuals reported sensations in the body. University of Leicester researchers also studied the emotions reported during hallucinations, with confusion, fear and frustration being the most common.

Although there was great variation in the localisation of feelings across participants, for each individual feelings were recurrently concentrated in particular body areas. Areas of concentration often held repeated sources of feelings like pain, heat, or tension.

Dr Katie Melvin, of the Department of Neuroscience, Psychology and Behaviour at the University of Leicester and corresponding author for the study, said:

“During a systematic review of existing research, we found indicators of the contributions that multiple senses, emotions and feelings may make to hallucinations.

“We designed a study and developed the novel but simple multimodal unusual sensory experience (MUSE) map method to investigate these features further. MUSE maps involve documenting hallucinations in daily life and include body-mapping. The article shares new insights through body-maps and data on the immediate feeling of hallucinations.

“The range of feelings in the body and around the body (into peri-personal space) were particularly interesting. Participants often described that the method helped them share experiences that were difficult to put into words.

“The methods and outcomes of this study can contribute to advances on how we understand hallucinations and how we can support people who experience them. The next steps for this area of research will be further understanding the embodiment and feeling of hallucinations in different populations and developing interventions to support with this.”

Psychosis is a term which describes experiences where an individual may have difficulties in determining what is real and what is not real.

Research indicates psychosis is associated with experiencing trauma, adverse life events, and stress. People may be given a diagnosis such as schizophrenia. Experiences of perceiving or believing things which those around us do not can also occur in physical health conditions such as brain tumours or acute infections.

Psychosis can have serious adverse outcomes on individuals including distress, lack of sleep, social withdrawal, lack of motivation, difficulties in carrying out daily activities, experiences of discrimination and lost opportunities.

Participants in this study were asked by the research team to prospectively document the feeling and senses of hallucinations for one week prior to an interview.

Novel visual diary methods involving drawing, writing and body-mapping generated 42 MUSE maps, which set out the specific areas across the body – and beyond, in so-called peripersonal space – where participants experienced sensations during hallucinations.

The study found that hallucinations were characterised by numerous feelings arising at once, often including multisensory, emotional, and embodied features. Researchers suggest further uptake of visual, ecological and prospective methods may enhance understandings of lived experiences of hallucinations.

Leicester’s Department of Neuroscience, Psychology and Behaviour brings together expertise in the fields of Neuroscience, Psychology, Clinical Psychology and Ophthalmology. Researchers work in partnership with University Hospitals of Leicester NHS Trust, and with Leicestershire Partnership Trust.

The feeling, embodiment and emotion of hallucinations in first episode psychosis: A prospective phenomenological visual-ecological study using novel multimodal unusual sensory experience (MUSE) maps’ is available in EClinicalMedicine, published by The Lancet.

WHAT COULD POSSIBLY GO WRONG

Teaching robots to think like us

Brain cells, electrical impulses steer robot though maze

Peer-Reviewed Publication

AMERICAN INSTITUTE OF PHYSICS

Robot experiments 

VIDEO: ROBOT EXPERIMENTS. A ROBOT WAS PLACED ON FIELDS WITH OBSTACLES AND WAS DIRECTED TOWARD THE GOAL. view more 

CREDIT: YUICHIRO YADA, SHUSAKU YASUDA, AND HIROKAZU TAKAHASHI

WASHINGTON, D.C., October 26, 2021 -- Can intelligence be taught to robots? Advances in physical reservoir computing, a technology that makes sense of brain signals, could contribute to creating artificial intelligence machines that think like us.

In Applied Physics Letters, from AIP Publishing, researchers from the University of Tokyo outline how a robot could be taught to navigate through a maze by electrically stimulating a culture of brain nerve cells connected to the machine.

These nerve cells, or neurons, were grown from living cells and acted as the physical reservoir for the computer to construct coherent signals.

The signals are regarded as homeostatic signals, telling the robot the internal environment was being maintained within a certain range and acting as a baseline as it moved freely through the maze.

Whenever the robot veered in the wrong direction or faced the wrong way, the neurons in the cell culture were disturbed by an electric impulse. Throughout trials, the robot was continually fed the homeostatic signals interrupted by the disturbance signals until it had successfully solved the maze task.

These findings suggest goal-directed behavior can be generated without any additional learning by sending disturbance signals to an embodied system. The robot could not see the environment or obtain other sensory information, so it was entirely dependent on the electrical trial-and-error impulses.

"I, myself, was inspired by our experiments to hypothesize that intelligence in a living system emerges from a mechanism extracting a coherent output from a disorganized state, or a chaotic state," said co-author Hirokazu Takahashi, an associate professor of mechano-informatics.

Using this principle, the researchers show intelligent task-solving abilities can be produced using physical reservoir computers to extract chaotic neuronal signals and deliver homeostatic or disturbance signals. In doing so, the computer creates a reservoir that understands how to solve the task.

"A brain of [an] elementary school kid is unable to solve mathematical problems in a college admission exam, possibly because the dynamics of the brain or their 'physical reservoir computer' is not rich enough," said Takahashi. "Task-solving ability is determined by how rich a repertoire of spatiotemporal patterns the network can generate."

The team believes using physical reservoir computing in this context will contribute to a better understanding of the brain's mechanisms and may lead to the novel development of a neuromorphic computer.

###

The article "Physical reservoir computing with FORCE learning in a living neuronal culture" is authored by Yuichiro Yada, Shusaku Yasuda, and Hirokazu Takahashi. The article will appear in Applied Physics Letters on Oct. 26, 2021. (DOI: 10.1063/5.0064771). After that date, it can be accessed at https://aip.scitation.org/doi/full/10.1063/5.0064771.

ABOUT THE JOURNAL

Applied Physics Letters features rapid reports on significant discoveries in applied physics. The journal covers new experimental and theoretical research on applications of physics phenomena related to all branches of science, engineering, and modern technology. See https://aip.scitation.org/journal/apl.

 NOT HORSE DEWORMER

Horse hyperimmune antibody may help the fight against COVID-19, Brazilian study finds


Peer-Reviewed Publication

INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA DE BIOLOGIA ESTRUTURAL E BIOIMAGEM (INBEB)

Horse inoculation 

IMAGE: TRIMERIC SPIKE GLYCOPROTEIN INDUCES HIGH LEVEL OF HORSE NEUTRALIZING ANTIBODIES. view more 

CREDIT: GUILHERME DE OLIVEIRA

A study conducted by a consortium of Brazilian researchers has demonstrated that a hyperimmune serum consisting of purified antibody fragments produced in horses may be an efficient approach to combat covid-19. Tests conducted in hamsters improved the animal clinical conditions. The neutralizing activity of the sera developed by the scientists has been proved to be high against the P.1 (Gamma) and P.2 variants. The results have been published in iScience.

The research opens up the possibility for the development of passive immunization or treatment against SARS-CoV-2 infection. “By using the trimeric spike protein of SARS-COV-2 to immunize horses, a cocktail of purified IgG fragments (F(ab’)2) showing very high neutralizing activity was developed. This might be a useful countermeasure against covid-19, with the added advantages of being an affordable alternative that can be produced in horse serum production facilities available worldwide,” says Professor Leda Castilho, from the Federal University of Rio de Janeiro, who coordinates the laboratory that produces the recombinant spike protein used for horse hyperimmunization.

The scientists are confident of the efficiency of the new product. “The main finding in our study is that the equine hyperimmune globulins antibodies developed against the spike protein of the ancestral SARS-CoV-2 had high neutralizing action against the new variants of the virus, such as the gamma strain. The polyclonal nature explains this high potency, which can be tailored now by using a combination of mutant spike protein from different variants as antigens,” says Professor Jerson L. Silva, from the Federal University of Rio de Janeiro, and coordinator of the study.

Equine immunization is a well-known and easily scalable technology proven to generate high titers of neutralizing antibodies, and has been used to treat many diseases, such as rabies, tetanus, and snake envenomation.

Since equine antivenom products are routinely produced in both high and low-income countries, the Brazilian strategy could be easily reproduced in any part of the world and could be rapidly tested as a therapy or passive immunization tool for covid-19.

The study

Previous works on equine hyperimmune sera against SARS-CoV and MERS-CoV had shown positive results for these closely related betacoronaviruses. Another recent study has shown that the recombinant receptor-binding domain (RBD) of the SARS-CoV-2 S protein could stimulate antibody production in mice and equines.

In the Brazilian study, the scientists used the recombinant trimeric spike (S) glycoprotein to immunize horses for the production of hyperimmune globulins against SARS-CoV-2. The technique combines the advantages of using an antigen that closely resembles its state on the surface of the native virus with the biosafety advantages related to using a recombinant immunogen.

After developing the horse hyperimmune F(ab’)2 concentrate, the group carried out challenge tests in hamsters, injecting the substance into the animals. The researchers evaluated the pretreatment and treatment after SARS-CoV-2 infection.

The results showed the ability of the treatment to reduce viral load in pulmonary tissues, and to improve the animal clinical conditions, as reflected by weight gain. All intervention schemes protected the animals against weight loss in the acute phase of the disease. Non-treated animals showed an increased weight loss, whereas pre-treatment resulted in no body weight reduction.

The next step of the research is to conduct a phase 1/2 clinical trial in humans. The trial protocol has already been approved by Brazilian national ethics committee, and sterile fill and finish is underway to enable quality control and batch release data to be submitted to the Brazilian regulatory agency ANVISA. The clinical trial will investigate as outcomes all safety aspects, as well as duration of hospitalization, viral load, and progression to assisted ventilation.

###

Additional information

The research consortium is formed by researchers from the Federal University of Rio de Janeiro, the Vital Brazil Institute, and the Oswaldo Cruz Foundation.

The paper “Polyclonal F(ab’)2 fragments of equine antibodies raised against the spike protein neutralize SARS-CoV-2 variants with high potency” is available online in iScience.

Funding

The study was funded by the National Council for Scientific and Technological Development (CNPq), the Brazilian Ministry of Science, Technology, and Innovation for Virus Network, the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro (FAPERJ), the Coordination of Improvement of Higher Education (CAPES/MEC) and the National Institute of Science and Technology for Structural Biology and Bioimaging (INBEB).

Neutron star collisions are “goldmine” of heavy elements, study finds


Mergers between two neutron stars have produced more heavy elements in last 2.5 billion years than mergers between neutron stars and black holes.


Peer-Reviewed Publication

MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Most elements lighter than iron are forged in the cores of stars. A star’s white-hot center fuels the fusion of protons, squeezing them together to build progressively heavier elements. But beyond iron, scientists have puzzled over what could give rise to gold, platinum, and the rest of the universe’s heavy elements, whose formation requires more energy than a star can muster.

A new study by researchers at MIT and the University of New Hampshire finds that of two long-suspected sources of heavy metals, one is more of a goldmine than the other.

The study, published today in Astrophysical Journal Letters, reports that in the last 2.5 billion years, more heavy metals were produced in binary neutron star mergers, or collisions between two neutron stars, than in mergers between a neutron star and a black hole.

The study is the first to compare the two merger types in terms of their heavy metal output, and suggests that binary neutron stars are a likely cosmic source for the gold, platinum, and other heavy metals we see today. The findings could also help scientists determine the rate at which heavy metals are produced across the universe.

“What we find exciting about our result is that to some level of confidence we can say binary neutron stars are probably more of a goldmine than neutron star-black hole mergers,” says lead author Hsin-Yu Chen, a postdoc in MIT’s Kavli Institute for Astrophysics and Space Research. 

Chen’s co-authors are Salvatore Vitale, assistant professor of physics at MIT, and Francois Foucart of UNH.

An efficient flash

As stars undergo nuclear fusion, they require energy to fuse protons to form heavier elements. Stars are efficient in churning out lighter elements, from hydrogen to iron. Fusing more than the 26 protons in iron, however, becomes energetically inefficient.

“If you want to go past iron and build heavier elements like gold and platinum, you need some other way to throw protons together,” Vitale says.

Scientists have suspected supernovae might be an answer. When a massive star collapses in a supernova, the iron at its center could conceivably combine with lighter elements in the extreme fallout to generate heavier elements.

In 2017, however, a promising candidate was confirmed, in the form a binary neutron star merger, detected for the first time by LIGO and Virgo, the gravitational-wave observatories in the United States and in Italy, respectively. The detectors picked up gravitational waves, or ripples through space-time, that originated 130 million light years from Earth, from a collision between two neutron stars — collapsed cores of massive stars, that are packed with neutrons and are among the densest objects in the universe.

The cosmic merger emitted a flash of light, which contained signatures of heavy metals.

“The magnitude of gold produced in the merger was equivalent to several times the mass of the Earth,” Chen says. “That entirely changed the picture. The math showed that binary neutron stars were a more efficient way to create heavy elements, compared to supernovae.”

A binary goldmine

Chen and her colleagues wondered: How might neutron star mergers compare to collisions between a neutron star and a black hole? This is another merger type that has been detected by LIGO and Virgo and could potentially be a heavy metal factory. Under certain conditions, scientists suspect, a black hole could disrupt a neutron star such that it would spark and spew heavy metals before the black hole completely swallowed the star.

The team set out to determine the amount of gold and other heavy metals each type of merger could typically produce. For their analysis, they focused on LIGO and Virgo’s detections to date of two binary neutron star mergers and two neutron star – black hole mergers.

The researchers first estimated the mass of each object in each merger, as well as the rotational speed of each black hole, reasoning that if a black hole is too massive or slow, it would swallow a neutron star before it had a chance to produce heavy elements. They also determined each neutron star’s resistance to being disrupted. The more resistant a star, the less likely it is to churn out heavy elements. They also estimated how often one merger occurs compared to the other, based on observations by LIGO, Virgo, and other observatories.

Finally, the team used numerical simulations developed by Foucart, to calculate the average amount of gold and other heavy metals each merger would produce, given varying combinations of the objects’ mass, rotation, degree of disruption, and rate of occurrence.

On average, the researchers found that binary neutron star mergers could generate two to 100 times more heavy metals than mergers between neutron stars and black holes. The four mergers on which they based their analysis are estimated to have occurred within the last 2.5 billion years. They conclude then, that during this period, at least, more heavy elements were produced by binary neutron star mergers than by collisions between neutron stars and black holes.

The scales could tip in favor of neutron star-black hole mergers if the black holes had high spins, and low masses. However, scientists have not yet observed these kinds of black holes in the two mergers detected to date.

Chen and her colleagues hope that, as LIGO and Virgo resume observations next year, more detections will improve the team’s estimates for the rate at which each merger produces heavy elements. These rates, in turn, may help scientists determine the age of distant galaxies, based on the abundance of their various elements.

“You can use heavy metals the same way we use carbon to date dinosaur remains,” Vitale says. “Because all these phenomena have different intrinsic rates and yields of heavy elements, that will affect how you attach a time stamp to a galaxy. So, this kind of study can improve those analyses.”

This research was funded, in part, by NASA, the National Science Foundation, and the LIGO Laboratory.

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Written by Jennifer Chu, MIT News Office

Paper: The relative contribution to heavy metals production from binary neutron star mergers and neutron star-black hole mergers”

https://iopscience.iop.org/article/10.3847/2041-8213/ac26c6