Showing posts sorted by relevance for query Homunculus. Sort by date Show all posts
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Tuesday, October 11, 2022

ON THE ROAD TO HOMUNCULUS

Brain-like organoids grown in a dish provide window into autism


The structures are reminiscent of one wrinkle of a human brain at 15 to 19 weeks post-conception.

Peer-Reviewed Publication

UNIVERSITY OF UTAH HEALTH

Lab-grown organoids reveal insights into the brain 

IMAGE: SESAME SEED-SIZED BRAIN-LIKE ORGANOIDS ARE GROWN IN THE LAB FROM HUMAN CELLS. THEY ARE PROVIDING INSIGHTS INTO THE BRAIN AND UNCOVERING DIFFERENCES THAT MAY CONTRIBUTE TO AUTISM IN SOME PEOPLE. view more 

CREDIT: TREVOR TANNER

(Salt Lake City) - Whatever you do, don’t call them “mini-brains,” say University of Utah Health scientists. Regardless, the seed-sized organoids—which are grown in the lab from human cells—provide insights into the brain and uncover differences that may contribute to autism in some people.

“We used to think it would be too difficult to model the organization of cells in the brain,” says Alex Shcheglovitov, PhD, assistant professor of neurobiology at U of U Health. “But these organoids self-organize. Within a few months, we see layers of cells that are reminiscent of the cerebral cortex in the human brain.”

The research describing the organoids and their potential for understanding neural diseases publishes in Nature Communications on Oct 6 with Shcheglovitov as senior author and Yueqi Wang, PhD, a former graduate student in his lab, as lead author. They carried out the research with postdoctoral scientist Simone Chiola, PhD, and other collaborators at the University of Utah, Harvard University, University of Milan, and Montana State University.

Investigating autism

Having the ability to model aspects of the brain in this way gives scientists a glimpse into the inner workings of a living organ that is otherwise nearly impossible to access. And since the organoids grow in a dish, they can be tested experimentally in ways that a brain cannot.

Shcheglovitov’s team used an innovative process to investigate effects of a genetic abnormality associated with autism spectrum disorder and human brain development. They found that organoids engineered to have lower levels of the gene, called SHANK3, had distinct features.

Even though the autism organoid model appeared normal, some cells did not function properly:

  • Neurons were hyperactive, firing more often in response to stimuli,
  • Other signs indicated neurons may not efficiently pass along signals to other neurons,
  • Specific molecular pathways that cause cells to adhere to one another were disrupted.

These findings are helping to uncover the cellular and molecular causes of symptoms associated with autism, the authors say. They also demonstrate that the lab-grown organoids will be valuable for gaining a better understanding of the brain, how it develops, and what goes wrong during disease.

“One goal is to use brain organoids to test drugs or other interventions to reverse or treat disorders,” says Jan Kubanek, PhD, a co-author on the study and an assistant professor of biomedical engineering at the U.

Building a better brain model

Scientists have long searched for suitable models for the human brain. Lab-grown organoids are not new, but previous versions did not develop in a reproduceable way, making experiments difficult to interpret.

To create an improved model, Shcheglovitov’s team took cues from how the brain develops normally. The researchers prompted human stem cells to become neuroepithelial cells, a specific stem cell type that forms self-organized structures, called neural rosettes, in a dish. Over the course of months, these structures coalesced into spheres and increased in size and complexity at a rate similar to the developing brain in a growing fetus.

After five months in the lab, the organoids were reminiscent of “one wrinkle of a human brain” at 15 to 19 weeks post-conception, Shcheglovitov says. The structures contained an array of neural and other cell types found in the cerebral cortex, the outermost layer of the brain involved in language, emotion, reasoning, and other high-level mental processes.

Like a human embryo, organoids self-organized in a predictable fashion, forming neural networks that pulsated with oscillatory electrical rhythms and generated diverse electrical signals characteristic of a variety of different kinds of mature brain cells.

“These organoids had patterns of electrophysiological activity that resembled actual activity in the brain. I didn’t expect that,” Kubanek says. “This new approach models most major cell types and in functionally meaningful ways.”

Shcheglovitov explains that these organoids, which more reliably reflect intricate structures in the cortex, will allow scientists to study how specific types of cells in the brain arise and work together to perform more complex functions.

“We’re beginning to understand how complex neural structures in the human brain arise from simple progenitors,” Wang says. “And we’re able to measure disease-related phenotypes using 3D organoids that are derived from stem cells containing genetic mutations.”

He adds that using the organoids, researchers will be able to better investigate what happens at the earliest stages of neurological conditions, before symptoms develop.


CAPTION

Single neural rosette-derived organoids develop multiple brain cell types and have an organization and neural activity never seen before in models of this kind.

CREDIT

Trevor Tanner

Single neural rosette-derived organoids model aspects of the brain (IMAGE)

UNIVERSITY OF UTAH HEALTH

Visit UBrain browser to visualize the cells and electrical responses detected in organoids.

The research published as “Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes.

Support for the work came from the National Institutes of Health, Brain Research Foundation, Brain and Behavior Research Foundation, Whitehall Foundation, University of Utah Neuroscience Initiative, and University of Utah Genome Project Initiative.

About University of Utah Health

University of Utah Health  provides leading-edge and compassionate care for a referral area that encompasses Idaho, Wyoming, Montana, and much of Nevada. A hub for health sciences research and education in the region, U of U Health has a $458 million research enterprise and trains the majority of Utah’s physicians and health care providers at its Colleges of Health, Nursing, and Pharmacy and Schools of Dentistry and Medicine. With more than 20,000 employees, the system includes 12 community clinics and five hospitals. U of U Health is recognized nationally as a transformative health care system and provider of world-class care.


Creating a mouse embryo from stem cells to learn more about the mammalian development process

Creating a mouse embryo from stem cells to learn more about the mammalian development process
Correct self-organization is necessary for proper morphogenesis. a, Time course of the
 assembly of ETX embryos stained to reveal E-cadherin (monochrome), 
Oct4 (red) and Gata4 (green). The bottom row of images are magnifications of the images
 above and show E-cadherin staining around a nascent cavity, as indicated by the dashed
 yellow lines. The dashed green line indicates the boundary between the ES and XEN 
compartment. Scale bar, 5 μm. b, Representative images showing Oct4 (red), 
Gata4 (green), E-cadherin (monochrome) and DAPI (gray) staining in day 4 cadherin
 OE ETX structures formed by combining E-cadherin OE ES cells with P-cadherin OE 
TS cells and wild-type XEN cells. ETX structures formed by combining wild-type cells 
were used as a control. Scale bars, 100 μm. c, Comparison and quantification of joined 
cavity formation in cadherin OE and control ETX structures. n = 361 (control group) and
 n = 253 (cadherin OE group). N = 5 for each condition. The data are presented as means 
± s.d. Statistical significance was determined by unpaired two-tailed Student’s t-test. d, 
Representative image showing Oct4 (red), Gata4 (green), laminin (monochrome) and 
DAPI (blue) staining in day 4 cadherin OE ETX structures formed by combining
 E-cadherin OE ES cells with P-cadherin OE TS cells and wild-type XEN cells.
 ETX structures formed by combining wild-type cells were used as a control. Scale bars, 
100 μm. e, Quantification of the structures that contained continuous or discontinuous 
laminin. n = 40 ETX structures per condition. N = 3. The data are presented as means 
± s.d. Statistical significance was determined by unpaired two-tailed Student’s t-test. f,
 Self-organization principles in stem cell-derived ETX embryos. Differential expression of
 E-, K- and P-cadherins enables the sorting of ES (epiblast-like), XEN (VE-like) and 
TS (TE-like) stem cells. Wild-type ES cells with low E-cadherin expression and wild-type 
TS cells with low P-cadherin expression exhibited detrimental global sorting efficiency. 
This could be overcome by overexpressing E-cadherin in ES cells and P-cadherin in TS
 cells to increase the efficiency of ETX embryo formation. Proper morphogenesis, including
 cavity formation, basement membrane formation (purple) and symmetry breaking can only
 be observed in well-sorted structures. Numerical data are available as source data. 
Credit: Nature Cell Biology (2022). DOI: 10.1038/s41556-022-00984-y

A team of researchers at the California Institute of Technology, working with one colleague from The Francis Crick Institute and another from the University of Cambridge, both in the U.K., has developed a way to grow mouse embryos without using mouse eggs or sperm to learn more about early mammalian development. In their paper published in the journal Nature Cell Biology, the group describes using several types of stem cells to grow mouse embryos.

Prior research has shown that mammalian embryos differentiate into different types of cell masses as they develop. Researchers have also found that  are involved in the processes but the mechanisms responsible are still unknown. In this new effort, the researchers used three different kinds of stem cells to grow a mouse embryo that matured to the point of having a  and the beginnings of a brain.

To create such embryos, the researchers first studied communications between stem cell groups in naturally developing . They learned to recognize the elements that went into such communications and the means by which it was carried out. In essence, they "deciphered the code." They then isolated three main types of stem cells that made up the cell masses in early embryo development: pluripotent, which eventually grow to become body tissue, and two other types that grew to become the amnionic sac and placenta. They also noted the quantities of each type of stem cell.

The next step was to attempt to create a mouse embryo from scratch using the three types of stem cells in a lab setting. With careful tending, the researchers grew an embryo that matured enough to allow for study of its development.

To test further, the researchers repeated the procedure but added genetically engineered cells to see how it impacted maturation of the embryo. They found they could replicate some of the same brain development issues that have been seen in . They suggest their work could also help explain what goes wrong when mice (or people) miscarry.

Stem cell biologists create new human cell type for research

More information: Min Bao et al, Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension, Nature Cell Biology (2022). DOI: 10.1038/s41556-022-00984-y
Journal information: Nature Cell Biology 
© 2022 Science X Network

Wednesday, July 06, 2022

RIMMER: No Laughing Matter – the unintended hilarity of Russia’ special military operation

RIMMER: No Laughing Matter – the unintended hilarity of Russia’ special military operation
Russia is not joking about in Ukraine, but their efforts to justify its invasion are risible. / wiki

By Julian Rimmer in London July 6, 2022

The Ukrainian War is no laughing matter, but the Russians are doing their level best to make it funny. As Larkin wrote, they may not mean to, but they do. While there is seemingly no aspect of this conflict that has not been subjected to forensic examination by the kommentariat in the last four months, observers have overlooked the comedy inadvertently provided by the Kremlin.

They shouldn’t.

The importance of humour as a means of undermining an authoritarian state cannot be overplayed. Totalitarian regimes despises jokes because each one is a tiny revolution, they crack the porcelain bauble of the dictator’s distended ego and undermine the authority he (and it’s always a he) projects. Sedition thrives amid the irony. Ridicule of the system weakened the Soviet Union and helped bring about its collapse.

Moreover, it’s comedy gold.

All of which more than justifies casting a scornful eye over the farce masquerading as a ‘special military operation’ and poking fun at the unintentional slapstick which has characterised the invasion of Ukraine.

Despite their fearsome reputation for martial prowess, Russian troops have seemed anything but when captured. Callow youths bawl and call their mums. Bravery would appear to be redefined as holding a man down while someone else tortures him, as if it were a Navalny protest, or trussing him up before applying similar techniques to those used at Katyn. The lasting image of this conflict will be Dagestani conscripts loading second-hand washing machines onto their tanks.

Russian Homunculus with Azovstal POW

Photos like the one above suggest recruiting sergeants have few criteria for selection other than the correct limb count. Every new military commander appointed by Putin looks fatter than his predecessor and whether it’s Shoigu, Dvornikov or Zidhko, the abdominal convexity of their uniforms suggests they have all eaten too many pirozhki. ‘Battle-hardened’ generals with enough medals pinned to their chest to cause a commodity supercycle have nothing more courageous on their CVs than Chechnya in the 1990s (they actually lost the first war), defeat of the tiny Georgian army in 2008, the border skirmish in Ukraine of 2014 and erm, the high-altitude, low-precision bombing of the somewhat less than invincible Syrian opposition forces in 2016.

There are, then, few honours on the escutcheon. Despite giving it the big ’un on the global stage for the last two decades, Russia’s military strategy looks to have been devised by Gengis Khan and if one extrapolates the ~30,000 lives expended to capture one fifth of Ukraine’s territory thus far there’ll be very few soldiers remaining to goosestep round Red Square next May 9th even if they succeed in denazifying and demilitarising the whole country.

Keyboard Warrior

Oscar Wilde quipped ‘One must have a heart of stone to read Little Nell without laughing’ and I feel exactly the same about former president Medvedev, that debonair badminton player, wearing military fatigues and sporting a beard on what looks suspiciously like a private jet.

The only thing more ludicrous was the image of that simian Kadyrov, whose fringe grows low on the forehead, wearing a pair of fifteen hundred-buck Prada boots – with stack heels.

These boots are made for walking – round a shopping mall.

And what about Putin, old football face himself? While his porcine eyes sink ever deeper into the sockets drilled into those puffy cheeks and jowls, the inscrutable expression prevails at the other end of a very long table. I’m reminded of the character in ‘Castaway’. Not Tom Hanks but Wilson the volleyball.

Old football face Putin

Kremlin propaganda is so hilariously cack-handed it’s more of a liability than an asset. Most of it makes perfect sense if you interpose the word ‘not’ into every sentence publicly uttered. Civilian deaths during the invasion are all elaborately stage managed and nothing more than provokatsiya. One suspects that if Putin were to wipe Ukraine off the map with a nuclear weapon, it would be dismissed as Russophobic provocation’ by the victims. The sinking of the Moskva was an accident. The withdrawal from Snake Island was a gesture of goodwill. Towns are rubbled in order to liberate them. Fifty years after Ben Tre in the Vietnamese War, Russians are deploying the same logic: ‘It became necessary to destroy Ukraine to save it’.

Of course, if you listen to Kremlin mouthpieces then sanctions are, of course, a mere fleabite to Putin’s puissance. Perhaps Russians are self-sufficient in fast food since they took over the McDonald's franchise. The food could not reasonably be expected to deteriorate. IKEA closed its business in Russia and I wondered if we should invade Ukraine on that basis? Will the Kremlin derive sufficient consolation from this to compensate for the loss of $250bn of currency reserves held offshore? Putin will insist this is a war of national security and so the money does not matter. In my experience, however, when people say it’s not about the money, it’s definitely about the money.

Still, the amount of energy expended explaining why sanctions have no impact implies they doth protest too much. Russian autos are now produced without airbags and if there’s one feature of a car I’d regard as essential in Russia, even more than the wheels, it’s the airbag. I also don’t understand why oligarchs allow the seizure of their yachts abroad. If your boat will be detained in the Med why not sail the Murmansk to Kamchatka route along Russia’s Arctic coastline? You’re safe from international piracy there.

If you like your comedy stand-up, though, there is nothing more gutbustingly hysterical than Russian television. There’s no need for light entertainment or sitcoms on the box when you have geopolitical talk shows occupying most of prime-time. These debates consist of swivel-eyed political scientists (a descriptor unknown elsewhere) and overweight, superannuated generals competing to make the most bloodcurdling threats about emptying Russia’s thermo-nuclear arsenal on the US, the EU or NATO. Light relief is provided by footage of the artillery bombardment of Ukrainian villages or the most offensive host complaining about the sequestration of his holiday apartments in Lake Como.

Weakest Link for Nutters

The only moments of fleeting goodwill are reserved for nostalgia about the Trump administration or hope that Tucker Carlson may one day replace him.

Laughter, then, holding up Putin to scorn and derision, is one of the best weapons at the West’s – and Ukraine’s disposal. Sooner or later, someone will update ‘Springtime for Hitler’ for his benefit. Dictators tend not to have a sense of humour because they lack the prerequisite self-awareness and cannot defend themselves against it. Humiliation is what they fear most. Through the tragedy, keep the guffaws coming. There’s no shortage of material.

Sunday, July 19, 2020

History of Alchemy from Early to Middle Ages


Term Paper (Advanced Seminar), 1997

13 Pages, Grade: 1.5 (A)

Free online reading

contents

Introductory remarks
1. The dawn of Alchemy
1.1. The word Alchemy
1.2. Legendary origins of alchemy
1.3. The oldest written sources
2. Alchemy in the West
2.1. Egypt
1.2. The Hellenistic era
2.3. The Arabic era
2.4. The Christian Middle Ages
3. Alchemy in the Far East
3.1. China
3.2. India and Tibet
Concluding remarks
Selected bibliography

Introductory remarks

This paper seeks to investigate the origins and the history of Alchemy. Almost no art or science has been subject to such controversial discussion over centuries than alchemy. It must be said that the alchemists themselves very much contributed to this controversy by keeping their recipes and practices secret. Because of the highly encrypted language and the excessive use of symbols and pictures in many alchemical treatises and works it really does not make wonder that alchemy became denounced as pseudo-science, deception and quite in a few cases even as folly. However, on the other hand there were a number of famous and learned men who seriously believed in alchemy and its possibilities. Also it should not be forgotten that alchemy was the mother of modern chemistry and many alchemists, though by mere chance, discovered on their quest for gold chemical processes and substances that are still in use. Mentioning gold is not to say that this was the one and only aim of all alchemists. As we shall see, alchemists throughout the world actually strove for three aims: wealth, i.e. making and multiplying of gold, silver, and precious stones; longevity, i.e. the panacea universalis, a substance that cures all illnesses; immortality, i.e. the elixir of life which restores youth at any given age.1
In this paper I will try to present the appearances of alchemy in a chronological order, however sometimes it will be necessary to abandon this way because of the parallel development of alchemy in very different regions. Also of interest will be some of the main theories and their development and finally important representatives of alchemy.

1. The dawn of Alchemy

1.1. The word Alchemy

Scholars are not yet sure where the word alchemy actually originates from. The Arabic prefix al- was put in front of an, apparently, much older word. Burckhardt2 traces chemy back to Old Egyptian k ê me/ch ê me = 'black soil' ,which is a name for Egypt, or just 'black' . An argument for that theory is that alchemy later sometimes was named Egyptian Art. According to other theories the word was coined by the Greek alchemists in Alexandria. In Greek it is P0:,^", meaning the stirring of the melted metal. It was then applied to the whole art. Yet another possible origin of the word is in the field of legends to which I will come in the following.

1.2. Legendary origins of alchemy

Basically there are three legends going around about the origin of alchemy. According to the first and perhaps most wide-spread one alchemy was brought into this world by the deity Hermes Trismegistos, who in the course of an ongoing syncretism in Hellenistic Egypt and Greece was equalled to the gods Chnum, Thoth and Ptah. On the basis of that legend alchemy is called the Hermetic Art and if something is sealed air- and watertight it is hermetically sealed.
According to an other legend alchemy, together with magic and other occult arts, came to mankind after the battle of the angels when the Lord threw the rebellious angels out of the heaven. They came down to earth, got married with ordinary women and taught them everything they knew. This knowledge was written down in a book called Chem~. 3 This legend is also apt to explain a possible origin of witches because this book is nothing less than the first Grimoire.4
Finally the third legend says that alchemy was taught to Moses and Aaron by the Lord himself because they were chosen. according to an apocryphal biblical story Moses destroyed the Golden Calf, burnt it to ashes, dissolved the ashes in water and gave the people of Israel to drink. Now, for someone who hears this story it must be quite obvious that if Moses was able to destroy gold so completely he must as well be able to make it.

1.3. The oldest written sources

Unfortunately there are only a few examples of early alchemical writings that have survived till today. Among these artefacts are some fragmentary cuneiform script tablets from old Egypt that contain recipes for making alloys and colouring metals. Burckhardt says that in Egypt alchemy was considered a holy art and therefore was handed down orally. The big fire in the library of Alexandria, too, surely destroyed a number of precious works on alchemy .
The so far oldest known and most famous sources are two papyri from the 3rd century AD. They became known as Papyrus Leidensis and Papyrus Holmiensis5. Both contain a number of recipes for the imitation of gold and silver by forming either gold-containing alloys or simply golden-looking ones like brass. Furthermore they contain recipes for 'making' precious stones and pearls. Some recipes are open deception6. It is held by some scholars that both papyri are compilations of much earlier works. For a very detailed description of the two papyri see the outstanding work of Lippmann. Another source is the tabula smaragdina, ascribed to Hermes Trismegistos. Its authenticity has been doubted at7 not only because the oldest preserved version is an Arabic translation. It contains a very dense summa of alchemy and because of this density is very difficult to understand. There is a number of other works dating back to that period and published under famous names, but most of these works are pseudepigraphs and therefore cannot be taken as a kind of 'authentic' sources.

2. Alchemy in the West

2.1. Egypt

It is very difficult to investigate early alchemy in Egypt, meaning pre-Hellenistic Egypt. Lippmann, Burckhardt et alii hold that here is the true cradle of alchemy, though in a wider sense. The oldest evidences can be traced back to the Old Kingdom (c. 3200 BC). As early as that the Egyptians were good metallurgists. However this metallurgy was in the hands of the priestly caste who claimed to have their knowledge right from the gods Ptah and/or Thoth8. The workshops were in the temples of these gods and the artisans were either priests themselves or slaves of the temple. Bearing this in mind indubitably must have been highly respected for their secret wisdom, a fact that reinforced their power.
Around 1000 BC one can find the first hints of a theory, that there are four elements, Fire, Water, Earth, Air, and everything consists of a mixture of them. If the mixture is changed it theoretically should be possible to transform one substance into an other. This theory usually is ascribed to Aristotle, but apparently similar theories, naming four and sometimes five elements, developed independently in different regions and cultures at about the same time. Also rather early emerged the concept of the seven planets and their corresponding metals, perhaps coming from Babylon.
In the course of time there developed a proper temple industry. They 'multiplied' gold by creating alloys, e.g. electron, with less valuable metals, were skilful in gilding objects for ritual purposes and even created alloys that merely looked like gold, e.g. brass. Apart from these metallurgical enterprises there also appeared the imitating of precious stones and the colouring of glass. The substances in use were mineral salts and certain ashes and slags. At quite an early stage the art of the priests became J,6<@B"D"*@J@H, i.e. was handed down from father to son in order to keep it secret. Burckhardt in particular explains the lack of written artefacts with this secretness.
However, step by step the Egyptian empire declined and foreign influences became stronger. Among those influences were all sorts of philosophical concepts and streams like platonic, pre-Socratic, and Aristotelian philosophy. A new age of alchemy was dawning.

1.2. The Hellenistic era

Several scholars and most encyclopaediae place the beginning of alchemy in this period. Indeed alchemy during this era reached a bright bloom. The centre of this new period became Alexandria with its great library. Characteristic of it was a heretofore unknown religious syncretism. As mentioned above, the Greek god Hermes Trismegistos in Hellenistic Egypt became mixed with the gods Chnum, Thoth, and Ptah and was equally worshipped. The Ouruboros, a snake that eats its own tail, is the symbol of two old Egyptian gods9 and at the same time of the Greek Agathodaimon, also a snake-shaped god. The latter later on was sometimes referred to as Egyptian philosopher, king, or god.10 The Egyptians readily accepted virtually every new philosophical idea and absorbed it into their own thinking, re-shaping and adapting it freely according to their needs. Among the important theories of the time is that of the ovum philosophicum, the egg that contains all four elements. Heraklitos and Xenophanes framed it in the formula ª< 6"Â B?< (one and all). Plato and Aristotle developed a transmutation theory on the basis of the four-element theory. A substance can be transformed into an other substance by adding or reducing certain features. For instance if you add heat to water it becomes steam, i.e. changes its state from liquid to gas. Likewise it must be possible to transmute base metals into silver or gold. The new concept here was that of the prima materia, the black original state of all matter without features. The process is described as follows: first reduce the base metal to the materia prima (black ash, coal, slag, blackness of crows), as the next step the salting ( taricheia, sepsis of Isis), Alloiosis and Metabolè under the influence of the sulphurs, salts, and 'waters' .11 In the phial there is a constant movement "<@(upward, male, active, fire, air, spirit of Mercury) and 6"J@ ( downward, female, passive, Water, Earth, spirit of Sulphur). The reaction of these two sprits creates the homunculus which is rising through the colours to gold. There were lots of imaginations about the time span required for the transmutation. Numbers vary between 9 hours, 7, 14, 21, 40, 41, 110 days and 4, 6, 9,12 months. Likewise there are to be found descriptions of 4 steps of transmutation (nigredo - black, reduction of the metal to the prima materiaalbedo - white, adding of purified mercury, citrinitas - yellow, adding of purified sulphur, rubedo - red, the mixture of the ingredients results in gold); 7 steps in connection with the seven planets(calcinatio - Mercury, putrefactio - Saturn, sublimatio - Jupiter, solutio - Moon, distillatio - Venus, coagulatio - Mars, extractio - Sun); 10 steps12, and 12 steps in correspondence to the zodiacal signs ( Aries - calcinatio, Taurus - congelatio, Gemini - fixatio, Cancer - solutio, Leo - digestio, Virgo - distillatio, Libra - sublimatio, Scorpio - separatio, Sagittarius - ceratio, Capricornus - fermentatio, Aquarius - multiplicatio, Pisces - proiectio). There co-existed the concepts of the tetrasomy ( copper, lead, tin, and iron as dead bodies), which is resurrected by the pneuma theion (divine spirit), and the three principles of Mercury (soul), Sulphur (spirit) and Sal (body), the trinity of which was called Androgyn. The most influential concept, however, was the idea of a substance which accelerates or causes transmutation at all, respectively, a substance known as xerion, elixir, philosophers' stone. Natural philosophy of that time held that all metals will one day reach the perfection of gold and that the seed of gold is in every metal. By means of the stone they sought to speed up nature and produce gold or silver in a much shorter time. Another belief was that base metals are 'ill' and have to be 'cured' with 'the stone'. From the 4th century onwards there developed a theory that mercury instead of gold is original part of all metals.
With the decline of the old religion there of course was connected a decline of the status of the priests. In reaction to this the priests adopted the claim of not creating substitutes of equal value but of making gold and silver itself. Under the influence of the younger Stoa superstition and mysticism rose. Alchemy became increasingly connected with magic and mancy. Alchemical treatises began to demand outer and inner purity. The adept should be free from envy, hatred and avarice. What's more, the adept must be chaste and for the time of the work keep a strict diet. Magical formulas and invocations together with purifying rituals emerged. An oath of secretness was established that ruled to speak about the art either not at all, or tecte (encrypted). The alchemist now had to observe the stars and wait for favourable constellations and some authors claimed, that the work must be begun on special days or in a special season. The methods and substances were being more and more obscured and the practical value of the writings gradually sank. As possible causes for the failure of the work were listed envy of demons, bad influence of the planets, the wrong season, ignorance or inappropriate use of the rituals and formulas.
Let me now come to some of the representatives of that period's alchemy and, in passing, their works. The first one to mention is Bolos of Mendes (around 200 BC). He brought with him the idea of the unity of the cosmos and interpreted the works of the Egyptian in this way. He seems to have been a practically working man because he wrote a book called 'Physica et Mystica' (title of the Latin translation). It contains a number of, rather obscure; recipes for the making of gold and colouring of metals. The next one is the disputed Hermes Trismegistos. between the 1st century BC and the 3rd century AD there was been published a vast number of works under the name of Hermes. The actual Corpus Hermeticum consists of 18 works, none of which really speaks about alchemy.
According to Haage the Corpus Hermeticum realiter is more a Gnostic than alchemical work and it merely was interpreted alchemically. The most tangible and historically authentic alchemist of the Hellenistic period, and perhaps its last great one, was Zosimos of Panopolis. He lived at the turn of the 4th to the 5th century. He was both a critical and productive author who built several apparatus himself. Zosimos was convinced of the possibility of transmuting base metals into gold by means of a certain substance. He described it as a dry, intensively red powder and called it P¬D4@< (xerion). His works are 'the divine water', 'of chemical devices and furnaces' ( to him goes back the Athanor13 ), 'of chemistry' and 'of the holy art'. In all his works he stresses that the way to the xerion leads via observation of and insight into nature. He also repeatedly pleads for keeping the art secret and encoded. Those who are really learned and chosen will be able to understand, those who aren't should leave the matter untouched.
Throughout the whole period there emerged lots of pseudepigraphs ascribed to Democritos, Isis, Maria Hebraica, Cleopatra and Hermes Trismegistos. A quite famous example for these pseudepigraphs is the chryspoiia by an author who called herself Cleopatra. It uses lots of pictures and symbols, among these the Ouruboros as symbol of the great work with the words ª< Jò B?< (one is all) inside. The writings of Synesius of Kyrene, Heliodoros, Dioskoros et alii have come down to us, however their historical existence is disputed. The preferred genres of these alchemical works were recipe, allegory, riddle poetry, visions of revelation, didactic poetry and letters, dialogue treatises, and commentaries.14
Evidently the 'making' of gold was very popular and successful in Hellas, because in 296 emperor Diocletian of Byzantium saw himself forced to order the burning of alchemical works, either because he feared that Alexandria could stand up against his kingdom, or he as a Christian deemed these writings heretical. In fact he might have been afraid of an economic chaos because of counterfeit money.

2.3. The Arabic era

While in Hellenistic Egypt and Greece alchemy was still in full bloom a new era was approaching with the expansion of the Arabic influence from Syria and Persia. The Arabs conquered huge areas from Asia via Northern Africa to Spain. Unlike other conquerors they did not destroy the culture and philosophy they encountered on their campaigns but treated them with great interest and respect. According to Lippmann no other expanding culture ever was so tolerant towards other cultures. Unavoidably they came across the blooming alchemy at the school of Alexandria and soon after (c. 7th century) the first translations of Greek works emerged, first in Syrian, later in Arabic. At their work the translators did not hesitate simply to take over Greek terms for devices and substances and prefix them with the Arabic al-15. The Arabs had a slightly more technical view on alchemy. Of course they also worked on transmuting base metals into gold but increasingly they discovered its use for medicine, If 'the stone' (al-iksir, elixir) is able to cure metals it must also be able to cure humans. The Arabic physicians dealt with the humoral pathology of Galenos, a concept on the basis of the four bodily liquids blood, black and yellow gall, and mucus. Galenos thought that if all these liquids are in equilibrium, man is healthy and even-tempered. He explains the human tempers16 with the gaining of the ascendancy of one of the four liquids. The medicine so far depended on herbal remedies. The experiments of the alchemists, however, gradually led to a pharmacopoeia of mineral remedies which step by step replaced the herbs. The same experiments with 'strong, i.e. corrosive, waters' several hundred years later led to the discovery of the mineral acids. The alleged founder of Arabic alchemy is Prince Khalid Jazid Ibn Mu'awija (635-704) of the Umaiyade dynasty. He strove in vain for the crown of the caliph and therefore he allegedly occupied himself with medical, astrological, and alchemical studies. He initiated the translation of several Greek works into Arabic. He gathered a number of scholars of his time at his court but had them incarcerated and executed because the promised transmutation always failed.
A shining authority of Arabic alchemy was Djabir Ibn Hajjan (c.720-819), in the newer research called Geber arabicus, in order to contrast him from Geber latinus who lived at the end of the 13th century. He advocated the experiment and practical work as the sources of theory. According to him mere brooding is futile. He developed a new theory of transmutation. At the beginning the matter must be reduced to the four elements. As a second step the ingredients must be brought into an equilibrium. For that purpose Djabir worked out a system of mathematical proportions that basically goes back to neo-Platonian and Pythagorean ideas. Now the matter must be reconstructed to the configuration of gold. He also worked on the concept of the lapis philosophorum. He defined it as trinity of soul, spirit and body in an equilibrium. In that state it is both volatile and constant, male and female, hot and cold, and moist and dry.
The very voluminous Corpus Gabirianum (8th-10th century) in two versions, one in 112 volumes, one in 70 volumes, is ascribed to him and a man called Al-Hasan ibn an-Nakid al-Mausili who lived in the middle/end of the 9th and the beginning of the 10th century. The long version of the Corpus Gabirianum is an extensive treatise on antique sciences in general. The 30 volumes that have come down to us contain a number of methods to create the elixir out of mineral and/or organic ingredients. The short version, apparently written by Djabir himself, consists of 70 volumes and contains an again extensive but complete and systematic description of the Djabirian alchemy. In these books Djabir repeatedly refers to the works of Greek authorities, not knowing that most of these are pseudepigraphs. Later ( in the 12th century) Gerhard of Cremona translated them into Latin and published the under the title Liber divinitatis de LXX.
Another very influential alchemist was Abu Bakr Muhammed ibn Zakarija 'ar-Razi (c.865-925) , often called Rhazes. He was a famous and successful physician and managed a hospital in Baghdad. He occupied himself with the Greek philosophy of nature and the Galenic humoral pathology and alchemy. In general he accepted Djabir's theories except his idea of the proportions of the ingredients. He again adopted a Sal-Sulphur-Mercury theory. During his practical studies he worked out a new classifying system. He distinguished between animal, vegetable, and earth-like matter and sub-divided the latter into volatile (spirits), metals (bodies), stones, vitriols, boraxes, and salts, thus making a big step towards systematic chemistry. Another theory of his was a corpuscular theory according to which all matter consists of atoms. The properties of the single matters depends from the density of the atoms - a very progressive view regarding the time when it was formulated. He perhaps did not know how close he was to truth. His main work was the Liber Secretorum 17 in which he laid down his theories and methods. Towards the end of his life he re-edited it and published an abridged version under the title Secretum Secretorum.
The latest one who deserves mentioning in the context of Arabic alchemy is Abu Ali al-Hussain Ibn Abdallah Ibn Sina (980-1037), latinized Avicenna. Like his colleague Rhazes he actually was a physician of high reputation. He studies the works of his predecessors and comes to believe that a real transmutation of metals is plainly impossible. Alchemy and the alchemists only can copy and imitate nature. He occupies himself with the elixir only for medical purposes. The search for gold has been abandoned for the sake of longevity. His main work Canon Medicinae (in the 12th century translated by Gerhard of Cremona) is one of the fundamental works of mediaeval medicine. A large number of pseudepigraphs was published under his name.

2.4. The Christian Middle Ages

During the 11th and 12th century Christian scholars became increasingly interested in science and philosophy of the Greek and Arab and thus became aware of alchemy as well. Cities like Paris, Salerno and Toledo became centres of education and science. Many philosophical and alchemical were translated into Latin, first of all in Spain and Sicily, then still occupied by the Arab. These works were regarded as the wisdom of 'the Old' and avidly studied, however some scholars, among them Adelard of Bath (1070-1146), stood up against the blind and un-reflected reception of the works. After their opinion one certainly should read these works but should not see in them the ultima ratio but rather a basis for own research. His contemporary Hortulanus wrote a compendium and a dictionary of alchemy and published a commentary of the tabula smaragdina. Alanus ab Insulis (1125- 1203) again wrote against the lack of scholarly self-consciousness he encountered among his contemporaries. He was abbot of Clairvaux and a very learned man. Because of his almost biblical age his contemporaries believed that he must have found the elixir. he wrote a book of recipes in rather obscure language. The books that by that time were read most were Djabir's Liber divinitatis de LXX, pseudo-Rhazes Liber lumen luminum and De aluminibus et salibus, a mixture of exoteric- scientific parts and esoteric - mystical allegories.
One of the greatest scholars of the middle Ages, Albertus Magnus (1193-1280), dedicated much of his life and work to alchemy. He, despite the works of Avicenna which he should have read, believed in the possibility of the transmutation and accordingly took effort in research. The essence of his theories and ideas he wrote down in his important works De Alchymia, De rebus metallicis et mineralibus, and Octo capita de philosophorum lapide. His contemporary Roger Bacon (1214-1292), like Albertus Magnus a very learned man18, had studied and taught at the important universities of his time. Bacon enjoyed a high reputation for trying out all experiments he read about. His concern for alchemy, however, caused him, he was member of the Franciscan order, several problems because about that time beginning in Spain alchemy became banned as un-Christian and pagan. In his main works Speculum AlchimiaeOpus tertium and his other writings he turned against the rising influence of occultism and magic19. He pleaded for alchemy as a serious science and a basis of philosophy of nature, and its practical side has nothing to do with mysticism and occult practices.
Notwithstanding the opposition of the clergy, alchemy became very popular during the 13th and 14th century. For the first time alchemy was included into encyclopaedias, e.g. De proprietatibus rerum by Bartholomaeus Anglicus (end of 12th century), Liber de natura rerum by Thomas Cantimpratensis(1201-1262), and Speculum maior by Vincenz of Beauvais, and lots of books with sometimes very obscure recipes were circulating. Allegedly alchemy also found its way into art and literature. According to Haage the Grail in Wolfram of Eschenbach's 'Parzival' is an allegory of the lapis philosophorum. This sounds intelligible because one of the properties both shared by the Grail and the philosophers' stone is to cure all diseases. The making and multiplying of gold and silver, like in the Hellenistic days, must have been very popular and profitable. The clergy increasingly frowned on this phenomenon until in 1317 Pope John XXII. issued the bull ' Spondent quas non exhibent ' which strictly prohibited counterfeiting.
At the end of this chapter the attention should be drawn to the work of Geber latinus (end of 13th-beginning of 14th century) . It is not completely proved yet, whether he really lived or not, however in his corpus there are some ideas that cannot have been those of Djabir Ibn Hajjan, alias Geber arabicus. His view of alchemy is that of an applied natural science and he is in favour of experiments rather than mere theory. his main works are S umma perfectionis magisterii, Liber de investigatione perfectionis, and Theorica et Practica. As sources for his Summa prfectionis magisterii the following works can be identified: Rhazes Liber Secretorum de voce Bubacaris, Geber arabicus Liber divinitatis de LXX, pseudo-Rhazes De aluminibus et salibus, pseudo-Aristotle De perfecta magisteria, Avicenna De congelatione et conglutinatione lapidum, pseudo-Avicenna De re recta, and Albertus Magnus De mineralibus. From these books and apparently after own studies Geber developed a new corpuscular theory and a new transmutation theory. To him all substances consist of corpuscles of different size which make them impure. Only if the alchemist is able to bring the substances into the state of mediocris substantia, i.e. all corpuscles are of the same size, they are fit for a transmutation. His recipe for a transmutation is as follows: 1. purify Mercury and Sulphur by sublimation, 2. fix the volatile Mercury by another sublimation, 3. sublime the Sulphur with iron and copper, and 4. bring the Mercury and the Sulphur, now both in mediocris substantia, to an reaction. As can be seen, the Sal is missing, to me a fact that clearly distinguishes him from Geber arabicus. Of his predecessors he picked up the idea that Mercury is a basic component of all metals and extended it to his theory of the lapis philosophorum being pure Mercury in mediocris substantia.
Like in all epochs before also in the Middle Ages of course there circulated pseudepigraphs in abundance. Again the names of the Greek philosophers, Hermes Trismegistos, but also of contemporary scholars like Arnaldus of Villanova and Raimundus Lullus, who somewhere in their lives occupied themselves with alchemy but never published own alchemical works, were to be found under sometimes obscure books of yet more obscure recipes.

3. Alchemy in the Far East

3.1. China

Let us now leave the West and direct our attention to the cultures of the Far East. Here in China and India also and alchemistic art developed but due to some cultural and historical peculiarities unfortunately the research so far did not quite succeed to detect the real origins of Eastern alchemy.
The roots of Chinese alchemy go far back into time, although most preserved written evidences are from the first few centuries AD. It can be held that they are compilations of much older works. An especially tragic date for the historians of China was the year 213 BC when emperor Shi-Huang-Ti ordered the burning of all books he could get, except those about farming, medicine, pharmacy, tree cultivation, and fortune-telling. Fortunately soon after him in the Han-period (205 BC-220 AD) there was much effort to replace the loss but lots of gaps evidently were carelessly filled with conjectures. Surely at these times forgery was booming.
The most striking difference to Western alchemy is that the Chinese give little or no importance to the making and multiplying of gold. The main goal of Chinese alchemy is the elixir of immortality. An argument for the old age Chinese alchemy is the belief in immortality which can be traced back to the 8th century BC, as early as in the 4th century BC it was believed that it is possible to attain immortality, and finally in the 1st century BC the corresponding drug was first mentioned in a treatise as 'drinkable gold'20. The Chinese philosophy already in ancient times developed the cosmic principle of Yin and Yang, represented by the symbol [. Either bears the seed of the other and neither could be without the other. The philosophy of nature is based on the concept of five elements (Fire, Water, Wood, Earth, Metal). In older research sometimes this is ascribed to Babylonian and/or Chaldean influences but more recent studies seem to have dropped that thought. Another philosophical root of Chinese alchemy is Taoism. This philosophical-religious movement was founded in the 6th century BC by Lao- Tse and its main work is the Tao-Te-King ('classic way of power'). From the beginning on the Taoists were outsiders and soon the movement split up into a purist and mystic fraction. The latter were said to possess super- natural powers and consequently the evolving alchemy was grafted onto them. Thanks to this and a collection called 'Yün chi ch'i ch'ien' ('seven tablets in a cloudy satchel') from 1023 AD we know some more about Chinese alchemy. The book 'Tan chin yao chüeh ('great secrets of alchemy'), ascribed to a certain Sun-Ssu -Miao (581-after 673), probably is the most famous Chinese alchemical work that has come down to us. It is a practical treatise on creating elixirs for attaining immortality, using organic and mineral ingredients (Mercury, Sulphur, salts of Mercury and arsenic are mentioned), and several recipes for the cure of diseases. Because some ingredients are highly poisonous it is no surprise that several monarchs died of elixir poisoning. Both alchemists and emperors became more cautious after a whole series of such royal deaths. In the centuries to come the Chinese presumably lost interest in alchemy because alchemical texts became more and more scarce and finally ceased completely.

3.2. India and Tibet

In India and Tibet there existed a kind of alchemy that strictly speaking was no alchemy in the true sense of the word but more or less pharmacy and, to apply the Paracelsian term, iatrochemistry. Lippmann reproaches especially Indian authors for a lack of chronological thinking. He criticises that newer findings simply were included into new editions of older works. Transmutation of metals into gold plays only a very marginal role in the older texts of that region. Immortality neither was a main objective of the Indians and Tibetans because their religions already offer a way to it. In India the Hinduistic god Shiva was thought to have invented alchemy, and Mercury, first mentioned in the Artha-sastra (4th-3rd century BC), was called the 'semen of Shiva'. In the oldest Indian texts, the Sanskrit Vedas, there are hints of alchemy close to that of China, and Lippmann thinks that there were mutual influences, at least after the rise of Buddhism. In Buddhist texts of the 2nd-5th century AD there emerges the idea of transmuting base metals into gold. According to Lippmann some alchemical knowledge was 'imported' from the West because the terms and processes are strikingly similar to Greek and Arabic ones. In Northern India up to the 8th century the existence of alchemy can be verified to a certain extent by Tibetan translations of alchemical texts, found in Buddhist stupas. These texts are about panaceae and transmutations of metals. As I said above, alchemy in India in fact was pharmacy. Accordingly the Indian 'alchemists' were mainly physicians. Unfortunately there are no names mentioned because almost every Indian alchemical work was published anonymously. They created very effective remedies using the salts of the metals, either of natural sources or chemically produced, sal ammoniac, sulphur, mercury, and other minerals. As a kind of 'spin-off' they also found methods for imitating precious stones and mixing colours for dyeing and colouring of cloth and, not to forget, make-up. With the rise of Tantrism between the 12th and 14th century alchemy, then surely influenced by the Western cultures, became associated with mysticism. Since there is no written evidence for alchemy after the mid of the 14th century it can be assumed that it died out as in neighbouring China.

Concluding remarks

In Europe alchemy did not simply fade away like in Asia. On the contrary during the renaissance and later in the 16th and 17th century it reached another climax. Generations of adepts were feverishly searching for the philosophers' stone and many of them ruined themselves completely. Nevertheless important inventions and discoveries were made, for instance the discovery of the mineral acids, the aqua vitae (alcohol), the Glauber salt, new methods for the production of steel, and so on. However, there were also very many cunning deceivers who roamed the land and pretended to be able to make gold. Many monarchs had their court alchemists because they hoped for an increase in their finance. Those court alchemists sometimes led comfortable lives but in many cases they also were hanged on the infamous gilded gallows when their patrons lost patience. A good example for recipes of the 17th century is a collection published 1992 by Heiko Skerra21. Parallel to the practical branch of alchemy there developed a mystical and occult movement. Secret societies and Hermetic orders emerged and worked out an inner alchemy. The transmutation was used by them as an allegory for the process of an inner catharsis the result of which should be an enlarged consciousness and a higher self.

Selected bibliography

Alchemie und die Alchemisten. Baden-Baden: AMORC-Bücher.1993
Brockhaus Enzyklopädie in vierundzwanzig Bänden: Neunzehnte, völlig neu bearbeitete Ausgabe. Erster Band A- APT, Mannheim: Brockhaus 1986
Burckhardt, Titus: Alchemie: Sinn und Weltbild. Neudruck Andechs: Dingfelder Verlag 1993
Haage, Bernhard Dietrich: Alchemie im Mittelalter; Ideen und Bilder - von Zosimus bis Paracelsus. Zürich/Düsseldorf: Artemis und Winckler, 1996
Lippmann, Edmund O. von: Entstehung und Ausbreitung der Alchemie. Mit einem Anhange: Zur älteren Geschichte der Metalle. Hildesheim/New York: Olms 1978
Schmieder, Karl Christoph: Geschichte der Alchemie. Halle 1832, Neudruck Langen: Roller 1987 Skerra, Heiko: Alchemie, der Stein der Weisen. Rhede(Ems):Ewert 1992
The New Enzyclopædia Britannica, Volume 25. Macropædia. Enzyclopædia Britannica, Inc. Chicago/Auckland/London/Madrid/Manila/Paris/Rome/Seoul/Sydney/Tokyo/Toronto
[...]

1 In different cultures these aims were different in their importance
2 Burckhardt, Titus: Alchemie: Sinn und Weltbild, 2. Aufl., Andechs:Dingfelder Verlag 1992
3 see also Lippmann, Edmund O. von : Entstehung und Ausbreitung der Alchemie. Mit einem Anhang: Zur älteren Geschichte der Metalle. Hildesheim/New York: Olms 1978, pp 310-312
4 secret book of magic, according to a wide-spread vernacular belief each witch possesses an own copy of it5 after the names of the libraries which keep them: Leiden and Stockholm
6 cf. Haage, Berhard Dietrich: Alchemie im Mittelalter, Ideen und Bilder - von Zosimus bis Paracelsus, Zürich /Düsseldorf: Artemis und Winckler, 1996, p. 70
7 Lippmann: Entstehung und Ausbreitung der Alchemie... p. 558 8 the god of fire and the god of wisdom
9 Apophis, the incarnation of darkness, and the Mhn snake, that protects the sun-god Re from Apophis10 it became custom to put living snakes into the foundations of a new temple
11 the hydor theion (divine water, spirit of mercury ), which resurrects the black prima materia
12 see Haage: Alchemie im Mittelalter... pp. 16-17
13 special kind of alchemical oven
14 cf. Haage: Alchemie im Mittelalter... p. 110
15 as in Al-chemy, Al-embic ( from ambix), Al-embroth ( sal divinum)
16 sanguine, choleric, melancholic, and phlegmatic
17 'secret' in this respect meaning 'technical knowledge, know how'
18 and therefore called doctor mirabilis
19 especially see his Epistola de secretis operibus artis et naturae et nullitate magiae
20 the term aurum potabile in the West refers to / is synonymous to panacea universalis
21 Alchemie, der Stein der Weisen. Rhede(Ems): Ewert 1992
13 of 13 pages
Details
Title
History of Alchemy from Early to Middle Ages
College
University of Leipzig
Course
Alchemy in Art and Literature
Grade
1.5 (A)
Author
Year
1997
Pages
13
Catalog Number
V94692
File size
366 KB
Language
English
Notes
Auf Wunsch liegt auch eine (gekürzte) deutsche Version vor.
Tags
History, Alchemy, Early, Middle, Ages, Literature
Quote paper
Torsten Bock (Author), 1997, History of Alchemy from Early to Middle Ages, Munich, GRIN Verlag, https://www.grin.com/document/94692