Tuesday, February 20, 2024

Israel’s statements on Brazil’s Lula are unacceptable, says Foreign Minister Vieira

After Brazil's President Luiz Inacio Lula da Silva (pic) on Sunday compared Israel’s war on Gaza to Hitler’s treatment of Jews, Israel said on Monday that Lula is not welcome in the Middle Eastern country until he takes back the comments. — Reuters pic

RIO DE JANEIRO, Feb 21 — The diplomatic spat between Brazil and Israel entered a third day yesterday, with Brazil’s foreign minister calling Israel’s response to comments made by President Luiz Inacio Lula da Silva on the Gaza Strip “unacceptable” and “untruthful.”

After Lula on Sunday compared Israel’s war on Gaza to Hitler’s treatment of Jews, Israel said on Monday that Lula is not welcome in the Middle Eastern country until he takes back the comments.

Yesterday, Brazilian Foreign Minister Mauro Vieira hit back at Israel. “For a foreign ministry to address a head of state from a friendly country in this way is unusual and revolting,” Vieira told Reuters and another news agency at the G20 summit in Rio de Janeiro.

“It is a shameful page in the history of Israel’s diplomacy,” Vieira said, adding that Israel tries to create a smokescreen to cover up what is happening in Gaza.

The Israeli government did not immediately respond to a request for comment on Vieira’s remarks outside of business hours.

Brazil said it does not intend to retract Lula’s comments.

Israeli Foreign Minister Israel Katz said on Monday that Israel “will not forget nor forgive,” calling Lula’s comments “a serious antisemitic attack” and saying that the Brazilian president is “persona non grata in Israel until he takes it back.” — Reuters

Gaza, Ukraine loom large as G20 foreign ministers meet

AFP
February 20, 2024

US Secretary of State Antony Blinken gestures on arrival at Brasilia Air Base on February 20, 2024, after landing for a three-day visit to the country 
- Copyright AFP EVARISTO SA


Joshua Howat BERGER, Louis GENOT

G20 foreign ministers open a two-day meeting Wednesday in Brazil, with the outlook bleak for progress on a thorny agenda of conflicts and crises, from the Gaza and Ukraine wars to growing polarization.

US Secretary of State Antony Blinken and Russian Foreign Minister Sergei Lavrov are both expected in Rio de Janeiro for the first high-level G20 meeting of the year — though not China’s Wang Yi.

In a world torn by conflicts and divisions, Brazil, which took over the rotating G20 presidency from India in December, has voiced hopes for what President Luiz Inacio Lula da Silva calls “the forum with the greatest capacity to positively influence the international agenda.”

But Lula’s bid to make the G20 a space for finding common ground suffered Sunday when the veteran leftist ignited a diplomatic firestorm by accusing Israel of “genocide,” comparing its military campaign in the Gaza Strip to the Holocaust.

The comments drew outrage in Israel, which declared him “persona non grata,” and could overshadow any bid to de-escalate the conflict via the G20.

“If Lula imagined he was going to propose peace resolutions on Israel or Ukraine, that just got swept off the table,” international relations specialist Igor Lucena told AFP.

More than four months after the Gaza war started with Hamas fighters’ unprecedented October 7 attack on Israel, which has vowed to wipe out the Islamist group in retaliation, there is little sign of progress toward peace.

A new UN Security Council resolution on a ceasefire was vetoed Tuesday by the United States, which said the text would endanger ongoing negotiations, including on the release of Hamas-held hostages.

The outlook is similarly downbeat on Russia’s war in Ukraine, which also has G20 members divided.

Despite a push from Western countries for the group to condemn President Vladimir Putin’s invasion, the G20’s last summit, held in New Delhi in September, ended with a watered-down statement that denounced the use of force but did not explicitly name Russia, which maintains friendly ties with fellow members like India and Brazil.

Underlining the G20 stalemate, the G7 group of top economies — Ukrainian allies Britain, Canada, France, Germany, Italy, Japan and the United States — will hold its own virtual meeting on the war Saturday, the second anniversary of Russia’s invasion.

– ‘Putting out fires’ –


Held at a marina on the Rio waterfront, the G20 meeting will open with a session on “addressing international tensions.”

The ministers will discuss global governance reform Thursday — a favorite issue for Brazil, which wants a greater voice for the global south at institutions like the UN, IMF and World Bank.

“The number and gravity of conflicts has returned to the level of the Cold War. That brings new urgency to the issue,” said Brazil’s top diplomat for G20 political negotiations, Mauricio Lyrio.

“We need to adapt the international system to prevent new conflicts,” he told journalists Tuesday. “Now, we’re just putting out fires.”

Brazil also wants to use its G20 presidency to push the fights against poverty and climate change.

There will also be space for bilateral meetings on the sidelines of the gathering — though a Blinken-Lavrov encounter looks unlikely, given the exploding tension over Russian opposition leader Alexei Navalny’s death in prison Friday.

Blinken and Lavrov last met in person at a G20 gathering in India in March 2023.

– Election-year havoc –


Founded in 1999, the Group of 20 brings together most of the world’s biggest economies.

Originally an economic forum, it has grown increasingly involved in international politics.

But the prospects for major advances via the group are dim in a year when elections will be held in some 50 countries, including key G20 members such as the United States and Russia, said Lucena.

“Reaching big agreements will be difficult,” he said.

“It’s not a favorable environment for resolving conflicts. On the contrary.”

A Brazilian government source said that after recent G20 struggles for consensus, the hosts axed the requirement that every meeting produce a joint statement — with the exception of the annual leaders’ summit, scheduled for November in Rio.

burs-jhb/bgs


Israel-Hamas War Splits G-20, Risking Paralysis at Meeting

Michael Nienaber, Samy Adghirni and Simone Iglesias
Tue, February 20, 2024 


(Bloomberg) -- The Group of 20 nations is so split on the conflicts in Gaza and Ukraine that they may be forced to reduce the forum’s scope and avoid geopolitical issues altogether this year, according to people familiar with the matter.

Removing all sensitive political topics from G-20 statements would diminish the relevance of the format, said one of the people, who spoke on the condition of anonymity. But that would give the group the chance on reaching consensus on other issues.

G-20 foreign ministers will meet in Rio de Janeiro starting Wednesday, when the group is expected to discuss the conflict in the Middle East. Complicating the upcoming gathering is the fact that Brazilian President Luiz Inacio Lula da Silva over the weekend compared Israel’s war on Hamas with Adolf Hitler’s extermination of Jews during the Holocaust.

Lula is setting the tone for developing nations since Brazil holds the rotating presidency of the G-20. Several Latin American countries have pulled their ambassadors from Israel, while South Africa has filed a lawsuit with the International Court of Justice, accusing Israel of genocide.

The Group of Seven represents the US and its main allies while the G-20 brings together countries from across the spectrum, including China, and so it becomes a focus for global disputes. Israel is not a member of the G-20.

In the run-up to the meeting of foreign ministers — as well as a gathering of the finance ministers next week — officials representing developing nations including South Africa and Brazil have said they want their position that Israel is committing genocide reflected in any joint G-20 statement, according to the people.

That wording has been rejected by several other G-20 nations including the US and Germany, the people said.

Brazil has explored strategies to keep the wars from overshadowing the rest of the agenda. These include potentially issuing a single statement at the end of Brazil’s presidency in November, rather than after each ministerial meeting, according to the people.

Some members representing the developing nations have argued that the G-20 should drop any references to geopolitical conflicts, including Russia’s war against Ukraine, since an agreement on those issues is seen as impossible, said the people. The upshot could be that any future G-20 statement will be shorter and less political.

Mauricio Carvalho Lyrio, the secretary for economic and financial affairs at Brazil’s foreign affairs ministry, told reporters on Tuesday that ministers participating in Rio’s meetings would issue a report rather than a statement.

“A declaration can’t be an end in itself,” he said. “There is an obsession to make statements, but sometimes they prevent progress in the discussions.”

Economic Focus


It also means that the G-20 format would re-focus on its initial aim of fostering economic cooperation and strengthening fiscal resilience to prevent a repeat of a global financial crisis, according to the people.

Still, Carvalho Lyrio insisted that geopolitics and crises would still be the main topic of discussion of Rio’s closed-meetings. Ministers will also focus on global governance reform, he said, which includes revamping institutions such as the International Monetary Fund and United Nations Security Council and is a key area of concern for host Brazil.

During a meeting in Morocco in October only a few days after Hamas launched its deadly attack on Israel, G-20 finance chiefs agreed on a communique that didn’t mention the conflict, underlining the forum’s struggle to address conflicts seen as threats to the global economy.

This followed a G-20 summit in India in September where leaders — after days of wrangling — managed to agree on compromise language on Russia’s invasion of Ukraine that won praise from the US and its allies but drew criticism from Kyiv.

Israel insists Hamas needs to be destroyed following the group’s Oct. 7 attack on southern Israeli communities, which killed 1,200 people. More than 29,000 have been killed in Gaza since Israel began its retaliatory offensive, according to health officials in the Hamas-run territory.

Hamas is considered a terrorist organization by the US, European Union and others.

--With assistance from Arne Delfs, Ramsey Al-Rikabi, Sylvia Westall and Andrew Rosati.

 Bloomberg Businessweek



Report: China disappointed over US veto on Israel-Hamas ceasefire vote

US Ambassador to the United Nations Linda Thomas-Greenfield casts a veto vote on a UN Security Council resolution to demand an immediate humanitarian ceasefire in Gaza, amid the ongoing conflict between Israel and Hamas, at UN headquarters in New York February 20, 2024. — Reuters pic

BEIJING, Feb 21 — China expressed “strong disappointment” over the United States blocking a draft United Nations Security Council resolution on the Israel-Hamas war calling for an immediate humanitarian ceasefire, Xinhua said today, citing its UN representative Zhang Jun.

The United States yesterday vetoed for the third time a draft United Nations Security Council (UNSC) resolution, blocking a demand for an immediate humanitarian ceasefire as it instead pushes the 15-member body towards a rival draft that calls for a temporary ceasefire linked to the release of hostages held by Hamas.

The US has said the draft resolution put forward by Algeria could jeopardize “sensitive negotiations” between US, Egypt, Israel and Qatar aimed at brokering a pause in fighting and securing the release of hostages.

“China expresses its strong disappointment at and dissatisfaction with the US veto,” Xinhua said, citing Zhang who urged the UNSC to push for a ceasefire calling it a “moral obligation that the council cannot shy away from”.

“The US veto sends a wrong message, pushing the situation in Gaza into a more dangerous one,” said Zhang, adding that objection to ceasefire in Gaza is “nothing different from giving the green light to the continued slaughter”.

Zhang said the spillover of the conflict is destabilising the Middle East region, raising risks of a wider war.

“Only by extinguishing the flames of war in Gaza can the world prevent the fires of hell from engulfing the entire region,” Xinhua cited him as saying

. — Reuters


The U.S. on Tuesday vetoed an Arab-backed United Nations Security Council resolution calling for a cease-fire in Gaza. But this time, Washington proposed calling for a temporary cease-fire and rejecting an Israeli ground offensive in Rafah without civilian protection. Patsy Widakuswara reports.

Objection to cease-fire in Gaza equals license to kill: Chinese envoy

(Xinhua09:20, February 21, 2024

UNITED NATIONS, Feb. 20 (Xinhua) -- Following the U.S. veto on a Security Council draft resolution that would have demanded an immediate humanitarian cease-fire in Gaza, a Chinese envoy said Tuesday that objection to a cease-fire in Gaza is nothing different from giving the green light to the continued slaughter.

The draft resolution won 13 votes in favor among the 15 members of the Security Council. Britain abstained.

China expresses its strong disappointment at and dissatisfaction with the U.S. veto, said Zhang Jun, China's permanent representative to the United Nations.

Algeria, on behalf of the Arab states, put forward the draft resolution demanding an immediate cease-fire in Gaza, the immediate release of all hostages, guaranteed access to humanitarian supplies, and the rejection of forced displacement. Such a resolution, based on the minimum requirements of humanity, is urgently required by the situation on the ground and deserves the support of all Security Council members, he said in an explanation of vote after the vote.

Algeria, demonstrating reason, sincerity, and an open attitude, held lengthy and extensive consultations with all parties on the draft resolution and took on board many constructive ideas, which made the draft resolution more balanced, he said. "The outcome of today's vote clearly shows that on the issue of a cease-fire to halt the fight in Gaza, it is not that the Security Council does not have an overwhelming consensus, but rather it is the exercise of veto by the United States that stifles the council consensus."

The U.S. veto sends a wrong message, pushing the situation in Gaza into a more dangerous one, said Zhang.

The U.S. claim that a resolution would interfere with the ongoing diplomatic efforts is totally untenable. Given the situation on the ground, the continued passive avoidance of an immediate cease-fire is nothing different from giving the green light to the continued slaughter, he said.

While the draft resolution has been vetoed, the spillover of the conflict is destabilizing the entire Middle East region, leading to rising risks of a wider war. Only by extinguishing the flames of war in Gaza can the world prevent the fires of hell from engulfing the entire region. The Security Council must act quickly to stop this carnage, said Zhang.

The Security Council must take action to push for a cease-fire. This should not be a matter of debate, but rather a moral obligation that the council cannot shy away from. It is a legal responsibility that the council must assume. Even more so, this is a political requirement that the council must fulfill in accordance with the UN Charter, he said.

"The veto cannot muffle the strong call for a cease-fire and an end to the war. The Security Council cannot stop its work to uphold justice and fulfill its responsibilities just because of the veto," he said.

China urges Israel to heed the call of the international community, abandon its plans for a Rafah offensive, and stop the collective punishment of the people of Palestine. China expects countries with significant influence to have fewer political calculations, but rather to be truly impartial and responsible, and to make the right choice to push for a cease-fire in Gaza. China calls on the international community to pool all diplomatic efforts to give the people of Gaza a chance to survive, give the people of the entire Middle East region a chance to have peace, and give it a chance for justice to be upheld, said Zhang.

(Web editor: Zhang Kaiwei, Zhong Wenxing)

Jessica Le Masurier reports on US veto of UNSC Gaza ceasefire resolution • FRANCE 24 English

 palestine palestinian gaza ceasfire israel protest demonstration

From River To Sea Both Should Be Freed From Hatred And Suffering – OpEd

By 

In Arabic, the word Jihad is a noun meaning the activity of “striving and/or persevering.” According to Prophet Muhammad there are two types of Jihad: minor and major. Once when Muslims were returning from a military expedition, which for Prophet Muhammad was a minor jihad. He said to the fighters that now they had to go through the major jihad. When Prophet Muhammad was asked what he meant by major jihad, he said it was the spiritual jihad. 

On another occasion, he said the real mujahid is the one who declares jihad against his/her carnal soul. (Tirmidhi). Exercising self-control and using willpower and reason to overcome one’s anger is described by Prophet Muhammad as “the major jihad.” Overcoming our own feelings of hatred, revenge and anger is much more difficult than overcoming our enemies.

In today’s world of fanaticism and extremism the words of Al-Ghazali, a 12th century Persian Muslim theologian, need to be repeated by all the world’s religious and political leaders: “Declare your jihad on thirteen enemies you cannot see – Egoism, Arrogance, Conceit, Selfishness, Greed, Lust, Intolerance, Anger, Lying, Cheating, Gossiping and Slandering. If you can master and destroy them, then you will be ready to fight the enemy you can see.”

Congresswoman Rashida Tlaib says, “From the river to the sea is an aspirational call for freedom, human rights, and peaceful coexistence, not death, destruction, or hate.” I say we can make it truly aspirational by making it focus on both people first and the land second. “From the river to the sea Palestinians and Israelis should be freed of hatred and suffering by ‘a two state for two peoples sharing of the land peacefully.'”

But the Hamas’ 2017 charter states that in principle, it “rejects any alternative to the full and complete liberation of Palestine, from the river to the sea.” Hamas opposes a two state solution, wants all the land between the Mediterranean Sea and the River Jordan, and violently opposed the Oslo peace accords negotiated by Israel and the Palestine Liberation Organization (PLO) in the mid-1990s. If the war ends with Hamas eliminated, and with a new Israeli government elected; there is the hope that the miracle of the Yom Kippur War may be repeated. 

On October 27, 1978, only five years after Egypt started the Yom Kippur War with a surprise  attack on Israel, Egyptian President Anwar Sadat and Israeli Prime Minister Menachem Begin were named winners of the Nobel Peace Prize for their progress toward achieving a Middle East accord. The Yom Kippur War was followed six years later by a Peace Treaty between Egypt and Israel. 

Could the same process follow the defeat of Hamas, and its opposition to a two state solution?  The only possible chance for avoiding more wars is the two state solution. To establish a Palestinian state alongside Israel. That will not be possible with the current leaders on either side. Extremists, both Israeli and Palestinian, will do all they can to sink the idea, as they have done since the 1990s. If this war does not deliver enough of a shock to break deeply-held prejudices and to make the idea of two states viable, nothing will. And without a mutually-acceptable way of ending the conflict, more generations of Palestinians and Israelis will be sentenced to more wars.

Although it might seem impossible now, I do believe that within a decade or two Muslims will visit Jerusalem and pray together with Jews as Prophet Isaiah states: “In that day there will be a highway from Egypt to Assyria. The Assyrians will go to Egypt, and the Egyptians to Assyria. The Egyptians and Assyrians will worship together. On that day Israel  will join a three-party  alliance with Egypt and Assyria, a blessing upon the heart. The LORD of Hosts will bless them saying, “Blessed be Egypt My people, Assyria My handiwork, and Israel My inheritance.” …(Isaiah 19:23-5) And then “Nation shall not lift sword against nation, neither shall they learn war anymore. (Isaiah 2:4)

For more than seven decades political nationalist leaders in Israel and Palestine have failed to find a way to end the conflict between their two peoples. Perhaps it is time for religious leaders who understand the religious importance of repentance, humility, forgiveness, compromise and hope for peace in overcoming more than seven decades of pain and anger. As the Qur’an states: “Perhaps Allah will put, between you and those to whom you have been enemies among them, affection. And Allah is competent, and Allah is Forgiving and Merciful.”  (60:7) Then the words of the Qur’an will be full-filled “From the depths of Darkness into the Light; for Allah is very kind and merciful to you.” (Qur’an 57:9)

There is much about the Gaza war that we still don’t know: how long it will last, what the death toll will be, how many hostages can be rescued or returned, and how successful Israel will be in its declared objective of destroying Hamas.

But so far the most important fact is that Iran isn’t getting what it wanted from the war. The real goal was to disrupt the gradual deepening of the strategic ties between Israel and its most important Arab neighbors. Tehran’s support for Bashar al-Assad in Syria is responsible for many times more deaths and refugees than all the Israeli-Palestinian wars combined. Indeed, Iran’s supreme leader Ayatollah Ali Khamenei told Hamas leader Ismail Haniyeh that Iran would not enter Hamas’ war with Israel; and accused the terror group of not giving Iran any prior warning of the October 7 attacks.

Dr. Mohamed Chtatou, a Professor at a university in Rabat, Morocco writes: “After the current (Hamas-Israel) war, Israel’s ultra-nationalist coalition will undoubtedly be undermined by public opinion, and probably by a commission of inquiry. If the Palestinian Authority were to agree to take over Gaza – backed by the international reconstruction aid that would inevitably arrive – and if a centrist coalition government were to emerge in Israel, everything would once again be possible. Two difficult “ifs”? Perhaps, but there is no serious alternative.”

The Qur’an refers to Prophet Abraham as a community or a nation: “Abraham was a nation/community [Ummah]; dutiful to God, a monotheist [hanif], not one of the polytheists.” (16:120) If Prophet Abraham is an Ummah; then fighting between the descendants of Prophets Ishmael and Isaac is a civil war and should always be avoided. And prior to the 20th century Arabs and Jews never did make war with each other. Even the surprise attack by Egypt and Syria of the Yom Kippur War was followed six years later by a Peace Treaty between Egypt and Israel. Could the same process follow the defeat of Hamas?

If all Arabs and Jews can live up to the ideal that ‘the descendants of Abraham’s sons should never make war against each other’ is the will of God; we will help fulfill the 2700 year old vision of Prophet Isaiah: “In that day there will be a highway from Egypt to Assyria. The Assyrians will go to Egypt, and the Egyptians to Assyria. The Egyptians and Assyrians will worship together. In that day Israel  will join a three-party alliance with Egypt and Assyria, a blessing upon the heart. The LORD of Hosts will bless them saying, “Blessed be Egypt My people, Assyria My handiwork, and Israel My inheritance.”…(Isaiah 19:23-5)

Prophet Muhammad said: “Should I not tell you what is better in degree than prayer, fasting, and charity.” They (the companions) said: “Yes.” He said: “Reconciling people, because grudges and disputes are a razor (that shaves off faith).” (Ahmad, Abu Dawood, and At-Tirmithi)

This is an excellent guide to dealing with the three-generation old Israeli-Palestinian conflict. Rather than focusing mostly on what the other side did to us, we all should focus on how the conflict has hurt all of us, and how much better our future would be if we could live next to each other in peace. If the descendants of Prophet Isaac and Prophet Ishmael negotiate a settlement that reflects the religious policy that “…there is no sin upon them if they make terms of settlement between them – and settlement [reconciliation and peace] is best.” (Quran 4: 128)  

Three thousand Hamas gunmen invaded southern Israel on October 7 2023, killing 1,200 people and taking another 253 back to Gaza as hostages. As of February 19, 2024, 30,000 Palestinians, including about 12,000 Hamas fighters killed in Gaza since Israel launched a major military campaign in response. 

If Hezbollah attacks Israel there will be two to three times more deaths. A Hamas official based in Qatar told Reuters that the terror group estimated it had lost 6,000 fighters during the four-month-old conflict, well below the 12,000 Israel says it has killed. The comments were a rare acknowledgment from the Hamas terror group that it has suffered significant losses and appeared to mark the first time that Hamas has differentiated between combatants and civilians in a death toll from the war.

Iran has warned its proxy Hezbollah not to give Israel cause to launch a full-scale war along the Israel-Lebanon border, fearing it would risk gains it believes Iran has made in the region since Hamas’s October 7 massacres in southern Israel.

The Nakba (catastrophe), the displacement of Palestinians during the 1948 establishment of the State of Israel and the War of Independence, could have been avoided if the Palestinian leadership had accepted the UN two state solution. Indeed, if the Palestinian leadership had accepted the British 1937 two state solution; millions of Jews would have been able to escape the Holocaust (catastrophe). Hopefully, the Palestinians will not make an all or nothing mistake again. 

A mid February national poll by Channel 12 news indicated that the current opposition, along with Benny Gantz’s National Unity party (previously in the opposition but now a member of the emergency government), could secure 75 of the Knesset’s 120 seats if elections were held today, with the bloc loyal to Prime Minister Netanyahu far behind at 45. Israel will be ready for a two state solution if the Palestinians desire an honest peace, meaning a total Peace to end the 83 years of conflict. 



Rabbi Allen S. Maller
Allen Maller retired in 2006 after 39 years as Rabbi of Temple Akiba in Culver City, Calif. He is the author of an introduction to Jewish mysticism. God. Sex and Kabbalah and editor of the Tikun series of High Holy Day prayerbooks.
Alberta government declares ‘early start’ to wildfire season


Connor O'DonovanVideo Journalist, Alberta Bureau
Published on Feb. 20, 2024, 

After the destructive wildfires in Canada in 2023, Alberta is getting a head start on preparations this year by declaring an 'early start' to the season

Alberta had a record wildfire year, what 2024 could hold for the province


Visit The Weather Network's wildfire hub to keep up with the latest on the 2024 wildfire season across Canada.

Alberta’s forestry minister has declared an "early start to wildfire season," announcing a number of new measures aimed at preparing for a potentially severe year.

“We have requested funding for an additional 100 wildland firefighters in budget 2024,” Todd Loewen, forestry minister, said Tuesday afternoon at a warehouse in Whitecourt, Alta.

RELATED: Alberta has dozens of wildfires still burning this winter. Here's why

“As of today, a permit is required for any burning planned in the forest protection area. This will reduce the likelihood of new, human-caused wildfires.”
(Alberta government/YouTube)

Alberta Wildfire typically defines the season as March through October. Loewen, though, says prevailing conditions are motivating the province to take a proactive stance, and it asked Albertans to do the same.

“We know we’re in an El Niño right now that’s been causing us to have warmer and drier temperatures overall. We’re hoping that comes to an end,” Loewen said.

“I urge Albertans, especially those who live in or near the forest protection areas, to become familiar with FireSmart principles and prepare their homes, properties and communities accordingly.”



Loewen stated thermal-imaging drones will be used to monitor fires following a pilot project in 2023, and nighttime helicopter operations will be expanded.

The forestry minister noted the 100 new positions would result in 1,000 firefighting personnel working by May 15.

Alberta is scheduled to release its 2024 budget next week.

 

Researchers are using RNA in a new approach to fight HIV


You know mRNA, now meet siRNA

Peer-Reviewed Publication

UNIVERSITY OF WATERLOO




Society learned about the value of mRNA during the COVID-19 pandemic when we saw scientists and medical professionals harness its power to deliver a vaccine for the virus within a year.

Now, University of Waterloo pharmacy associate professor Emmanuel Ho has developed a novel nanomedicine loaded with genetic material called small interfering RNAs (siRNA) to fight human immunodeficiency virus (HIV) using gene therapy. These siRNAs regulate which genes or proteins are turned on or off in our cells and showed a 73 per cent reduction in HIV replication.

“This opens the door for new therapeutics in the fight against HIV,” said Dr. Ho, who is among Waterloo’s researchers and entrepreneurs leading health innovation in Canada.

Autophagy, also known as the body’s recycling process, plays an important role in our body to eliminate microbes such as viruses and bacteria inside cells. HIV is quite smart and produces a protein, Nef, that prevents cells from activating autophagy.

This is the first research to develop a combination nanomedicine that can reactivate autophagy and prevent HIV entry into cells, allowing our body to re-initiate its defence system.

Additionally, HIV has a gene, CCR5, that allows the virus to enter a cell. The siRNAs target both Nef and CCR5 to reduce HIV infection. 

This nanomedicine is intended to be applied vaginally to protect against sexual transmission of HIV. As a result, the nanomedicine is designed to be stable without leakage of siRNAs in the acidic vaginal environment but release the siRNA once inside cells.

“Viruses are smart. They produce Nef proteins to prevent autophagy from occurring,” Ho said. “Our process allows our body to fight the viral infection without needing additional drugs,” 

Ho confirms that the next steps include further optimizing the process and improving our understanding of how autophagy plays a role in how our cells protect us from viruses.

“We also hope this will shed some light to develop more alternative approaches to effectively reduce antimicrobial resistance,” Ho said.

The study, pH-sensitive dual-preventive siRNA-based nanomicrobicide reactivates autophagy and inhibits HIV infection in vaginal CD4+ cells, was recently published in the Journal of Controlled Release.

 

Annual breast cancer screening beginning at 40 saves lives


Peer-Reviewed Publication

RADIOLOGICAL SOCIETY OF NORTH AMERICA




OAK BROOK, Ill. – Annual breast cancer screening beginning at age 40 and continuing to at least age 79 results in the highest reduction in mortality with minimal risks, according to a new study published today in Radiology, a journal of the Radiological Society of North America (RSNA).

Breast cancer is the second most common cause of cancer death for women in the U.S. Despite research demonstrating that consistent participation in screening mammography can reduce breast cancer deaths by 40%, only 50% or less of eligible women actually participate in annual screening.

“There is an ongoing debate over the recommendations for breast cancer screening, specifically about when to start and the frequency of screening,” said lead researcher Debra L. Monticciolo, M.D., professor of radiology at Dartmouth Geisel School of Medicine in Hanover, New Hampshire.

Dr. Monticciolo said a recommendation by the U.S. Preventive Services Task Force (USPSTF) in 2009 to screen every other year, or biennially, beginning at age 50 resulted in a nationwide decline in screening participation. The USPSTF drafted new recommendations in 2023, suggesting women participate in biennial screening between 40 and 74. The American College of Radiology, the Society of Breast Imaging and the National Comprehensive Cancer Network recommend annual screening for women at average risk for breast cancer beginning at age 40 and continuing as long as the woman is in good health.

In the study, Dr. Monticciolo and colleagues performed a secondary analysis of Cancer Intervention and Surveillance Modeling Network (CISNET) 2023 median estimates of breast cancer screening outcomes. CISNET modeling data gives researchers the opportunity to estimate the outcomes of screening at various frequencies and starting ages using U.S. data.

The researchers compared the benefits of screening, including mortality reduction, life years gained, breast cancer deaths averted, and its risks—including benign, or unnecessary, biopsies and recall rates—for four different scenarios: biennial screening of women 50-74 (the longstanding USPSTF recommendation), biennial screening of women 40-74 (the task force’s new draft recommendation), annual screening 40-74, and annual screening 40-79. CISNET does not offer modeling past age 79.

The review of CISNET estimates showed that annual screening of women 40-79 with either digital mammography or tomosynthesis showed a mortality reduction of 41.7%. Biennial screening of women 50-74 and 40-74 showed mortality reduction of 25.4% and 30%, respectively. Annual screening of women 40-79 years showed the lowest per mammogram false-positive screens (6.5%) and benign biopsies (0.88%) compared to other screening scenarios.

“The biggest takeaway point of our study is that annual screening beginning at 40 and continuing to at least age 79 gives the highest mortality reduction, the most cancer deaths averted, and the most years of life gained,” Dr. Monticciolo said. “There’s a huge benefit to screening annually until at least 79 and even more benefit if women are screened past 79.”

Dr. Monticciolo said that although the USPSTF uses CISNET modeling to formulate its recommendations, it refers to recall rates and benign biopsies as harms, rather than risks.

“To balance the harms and benefits of screening mammography, they’re willing to give up some mortality benefit to avoid women being recalled for additional imaging and benign biopsies,” she said. 

According to the researchers’ analyses, the chance of a woman having a benign biopsy following annual screening is less than 1%, and all recall rates for screening mammography are under 10%. When screening is performed annually with tomosynthesis, the recall rate decreases to 6.5%.

“The risks of screening are non-lethal and manageable for most women,” she said. “But advanced breast cancer is often lethal. Breast cancer is easier to treat if it’s found earlier; we’re able to spare women extra surgeries and chemotherapy. It’s just a better idea to shift to early detection, and that’s what screening does.”

Dr. Monticciolo said she hopes that her study will add to the body of literature that supports annual screening beginning at age 40 as the best way to diagnose cancer early.

“This paper is important because it shows once again that there’s a tremendous increase in mortality benefit by screening annually between the ages of 40-79, and that the chances of experiencing harm are low on a per-exam basis,” she said. “It comes down to valuing women’s lives. I am hoping that primary care physicians see that risks of screening are manageable, and the benefits are tremendous. We need to do this for women.”

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“Outcomes of Breast Cancer Screening Strategies Based on Cancer Intervention and Surveillance Modeling Network Estimates.” Collaborating with Dr. Monticciolo were R. Edward Hendrick, Ph.D., and Mark A. Helvie, M.D.

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Bar-Ilan University researchers produce “laboratory testicles”


The artificial testicles will contribute to the advancement of research in male fertility and sexual development disorders, and are expected to facilitate the eventual production of sperm in the laboratory


Peer-Reviewed Publication

BAR-ILAN UNIVERSITY

Embryonic testis organoids bright field 

IMAGE: 

A BRIGHT FIELD IMAGE OF A TESTICULAR ORGANOID CREATED FROM MOUSE EMBRYOS AND INCUBATED IN A DISH FOR 14 DAYS. THE TUBULAR STRUCTURES FORMED IN THE DISH ARE CLEARLY VISIBLE. 

 

 

 

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CREDIT: CHELI LEV




The testis is responsible for sperm production and testosterone synthesis. Abnormalities in testis development and function lead to disorders of sex development (DSD) and male infertility. Currently, no in vitro system exists for modeling the testis.

 

Dr. Nitzan Gonen, a researcher specializing in the process of fetal sex determination, together with research students Aviya Stopel, Cheli Lev and Stav Dahari, has succeeded in creating "laboratory testicles" that may significantly advance understanding of the mechanisms involved in sex determination and provide solutions for male infertility, which affects one in 12 men worldwide.

 

The artificial testicles produced in Dr. Gonen’s lab at the Goodman Faculty of Life Sciences and the Institute of Nanotechnology and Advanced Materials at Bar-Ilan University, are testis organoids – tiny, artificial organs produced from real mouse testis. Development of organoids has greatly advanced in the last decade with the realization that two-dimensional cellular sample in vitro cannot mimic the behavior of an entire organ. Today, organoids of the brain, kidneys, intestines, and other organs have already been produced. The testicular organoids created by Gonen's group closely simulate a natural testicle.

 

The artificial testicles were cultured from immature testicular cells sampled from neonatal mice. The research team realized the procedure was a success when they identified tubule-like structures and cellular organization highly resembling that of the in vivo testis. These tubular structures parallel the multiple seminiferous tubules present in the natural testicle, where the sperm is produced.

 

The organoids were successfully cultured in vitro for nine weeks. This is considered a long period of time and can, theoretically, be enough time to complete the process of sperm production and hormone secretion. In mice this takes 34 days, so the relatively long lifespan of the organoids may allow these processes to occur in vitro. Dr. Gonen doesn’t yet know if the existing model will actually produce sperm cells, but the laboratory team has already noticed signs of the beginning of meiosis, a process in which gametes are produced. Gametes are reproductive cells, in this case sperm cells with half the number of chromosomes as in a normal cell, that "await" for the completion of the other half from another gamete, in this case an egg, upon fertilization.

 

Organoids usually resemble organs in the embryonic stage. In this case the researchers created conditions that allowed the organoid to mature in the laboratory and showed that even testicles grown from embryonic cells can develop and grow clear sperm tubes. The team was unsuccessful in its attempt to grow organoids from adult mice testis.

 

“Artificial testicles are a promising model for basic research on testicle development and function, which can be translated into therapeutic applications for disorders of sexual development and infertility,” explains Dr. Gonen. In the future she plans to produce organoids using human samples. A testis produced from human cells, for example, could help children being treated for cancer, which may impair their ability to produce functional sperm. As children are too young to produce their own sperm, these samples can be frozen and used in the future to have children. Gonen’s vision is to grow testes organoids from biopsies of children with cancer and hopefully grow fertile sperm in vitro.  

 

The finding of this study were recently published in the International Journal of Biological Sciences.