Storage process: a new method reduces the acute toxicity of the essential oil of Artemisia argyi H. Lév. & Vaniot by 40%
Xia & He Publishing Inc.
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Artemisia argyi H. Lév. & Vaniot essential oil reduces its acute toxicity while enhancing its antioxidant and analgesic activities after storage. This demonstrates the feasibility of reducing toxicity while enhancing bioactivity through storage and offers valuable insights for the detoxification of other essential oil products.
view moreCredit: Dingkun Zhang, Ming Yang
Background and objectives
Artemisia argyi H. Lév. & Vaniot essential oil (AAEO) holds significant pharmacological potential, but its application is constrained by hepatotoxicity. This study aimed to investigate the feasibility of reducing AAEO’s toxicity through storage and to evaluate changes in chemical composition, toxicity, and bioactivity.
Methods
Gas chromatography-mass spectrometry was used to analyze compositional changes during storage. Zebrafish acute toxicity tests and the liver-specific transgenic zebrafish model Tg(fabp10:EGFP) were used to assess toxicity. Antimicrobial, analgesic, and antioxidant assays evaluated variations in bioactivity.
Results
Over the 150-day storage period, gas chromatography-mass spectrometry analysis identified 39 components. Zebrafish acute toxicity tests showed that the LD50 of AAEO stored for 0, 30, 60, 90, 120, and 150 days were 0.10 µL·mL−1, 0.10 µL·mL−1, 0.10 µL·mL−1, 0.11 µL·mL−1, 0.13 µL·mL−1, and 0.14 µL·mL−1, respectively, demonstrating a 40% reduction in acute toxicity after 150 days of storage. Using the liver-specific green fluorescent transgenic Tg(fabp10:EGFP) zebrafish model, the inhibition rates of AAEO on hepatic fluorescence intensity were measured at 68.5%, 43.5%, 42.6%, 37.8%, 34.6%, and 31.9% at different time points, confirming reduced hepatotoxicity after storage. Additionally, the antioxidant and analgesic activities of AAEO were significantly enhanced (p < 0.05) after storage, while the antibacterial activity decreased (p < 0.05).
Conclusions
After storage, AAEO significantly reduces hepatotoxicity, with a 40% decrease in acute toxicity after 150 days. Meanwhile, the antioxidant and analgesic activities of AAEO increase, while its antibacterial activity decreases after storage.
Full text:
https://www.xiahepublishing.com/2835-6357/FIM-2025-00018
The study was recently published in the Future Integrative Medicine.
Future Integrative Medicine (FIM) is the official scientific journal of the Capital Medical University. It is a prominent new journal that promotes future innovation in medicine.It publishes both basic and clinical research, including but not limited to randomized controlled trials, intervention studies, cohort studies, observational studies, qualitative and mixed method studies, animal studies, and systematic reviews.
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Journal
Future Integrative Medicine
Article Title
Storage Process: A New Method Reduces the Acute Toxicity of the Essential Oil of Artemisia argyi H. Lév. & Vaniot by 40%
Article Publication Date
30-Jun-2025
Lavender steps up as a natural preservative in skin-care emulsions
Journal of Dermatologic Science and Cosmetic Technology
Synthetic parabens and formaldehyde releasers are falling out of favour, but keeping creams safe from microbes remains a challenge. The global shift toward “clean-label” cosmetics has left formulators scrambling for milder preservatives. A Research Paper led by Dr Trapali (University of West Attica, Greece), now offers a drop-in solution: the simple pairing of Lavandula angustifolia hydrosol with its own essential oil.
Using standard O/W emulsions, the researchers challenged products with high loads of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. After 12 weeks at 25 °C and 40 °C, emulsions containing 0.05 % hydrosol + 0.05 % essential oil kept counts below 10 CFU/mL—well inside European Pharmacopoeia limits. In contrast, an unpreserved control passed 10⁵ CFU/mL within four weeks.
Six O/W emulsions were stored at 25 °C and 40 °C for 12 weeks. Products containing 0.05 % lavender essential oil plus 0.05 % hydrosol remained below 10² CFU g⁻¹, while an unpreserved control exceeded 10⁴ CFU g⁻¹ by week four. The authors also provide a rapid GC-MS protocol to ensure consistent linalool levels across lavender chemotypes.
The work is the first to document a true synergy between lavender hydrosol and essential oil, outperforming either agent alone.
Journal
Journal of Dermatologic Science and Cosmetic Technology
Method of Research
Experimental study
Subject of Research
Not applicable
Article Title
Evaluation of the efficacy of lavender formulations as preservative agents in O/W (oil-in-water) emulsions
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