Friday, January 19, 2024

 

A more eco-friendly facial sheet mask that moisturizes, even though it’s packaged dry



Peer-Reviewed Publication

AMERICAN CHEMICAL SOCIETY





Starting a new year, many people pledge to enact self-care routines that improve their appearance. And facial sheet masks soaked in skin care ingredients provide an easy way to do this. However, these wet masks and their waterproof packaging often contain plastics and preservatives. Now, a study in ACS Applied Materials & Interfaces reports a dry-packaged hydrating facial mask that is made of biobased and sustainable materials.

Consumers in the beauty industry are increasingly concerned about the sustainability and sourcing of personal care items, in terms of both products’ ingredients and packaging. Facial sheet masks are popular cosmetic products advertised to benefit and enhance the skin. But they are typically made with plastic backing fabrics and are packaged with wet ingredients, requiring preservatives and disposable water-tight pouches. A more environmentally friendly option would be to package the facial masks dry. So, Jinlain Hu and coworkers aimed to design a facial sheet mask with biobased materials that could be enveloped in paper and later activated to deliver moisture and nutrients.

The researchers developed a facial mask with a sheet of plant-based polylactic acid (PLA), which could repel water, and they coated it in a layer of gelatin mixed with hyaluronic acid and green tea extract. They deposited the top layer as either tiny fibers or microspheres, using electrospinning or electrospray, respectively, and tested how well the masks could transfer moisture. They found:

  • Water droplets did not pass through the masks without skin contact, regardless of which side a water droplet was placed on.
  • Contact with skin initiated one-way water transport from PLA to gelatin to skin, but only for masks coated with gelatin-based microspheres.
  • Placing the mask on moistened, rather than dry, skin improved water delivery through the mask.

Finally, the team investigated how its mask’s ingredients impacted mouse cells as a proxy for reactions on skin. Fewer cells showed signals of aging when grown on the mask compared with cells grown in control conditions; the researchers attribute this to the antioxidant properties of the green tea extracts. The team says the beneficial properties of the natural ingredients and the one-way moisture-delivery design make this mask a promising alternative with a lesser environmental impact compared to traditional, wet-packed products.

The authors acknowledge funding from the City University of Hong Kong, the National Natural Science Foundation of China, and Shenzhen-Hong Kong-Macau Science & Technology Fund.

###

The American Chemical Society (ACS) is a nonprofit organization chartered by the U.S. Congress. ACS’ mission is to advance the broader chemistry enterprise and its practitioners for the benefit of Earth and all its people. The Society is a global leader in promoting excellence in science education and providing access to chemistry-related information and research through its multiple research solutions, peer-reviewed journals, scientific conferences, eBooks and weekly news periodical Chemical & Engineering News. ACS journals are among the most cited, most trusted and most read within the scientific literature; however, ACS itself does not conduct chemical research. As a leader in scientific information solutions, its CAS division partners with global innovators to accelerate breakthroughs by curating, connecting and analyzing the world’s scientific knowledge. ACS’ main offices are in Washington, D.C., and Columbus, Ohio.

To automatically receive news releases from the American Chemical Society, contact newsroom@acs.org.

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UK 

New PhD. training centers for flooding, mineral resources, freshwater quality and wetlands


Grant and Award Announcement

UK RESEARCH AND INNOVATION




 

UK invests in a new generation of environmental scientists

Four new centres for doctoral training will boost UK expertise in flood management, wetland restoration, sustainable mineral resources and freshwater quality.

The new Natural Environment Research Council-funded centres will teach the next generation of PhD students who will go on to build careers in research, business and public service. Each centre will be supported with £2.6 million funding.

The centres for doctoral training  (CDTs) will focus on following themes:

#Flooding

Millions of homes and businesses are at risk of flooding in the UK from rivers, the sea or surface water, with billions more affected globally.

A new CDT led by the University of Southampton will improve understanding of: drivers of flooding, for example, from rainfall, enhanced river discharge, surface runoff and storm surges; and climate change, changing population and development, and public perceptions affect flood risk.

The new cohort of researchers will develop new approaches to map and forecast floods, including using state-of-the-art monitoring and advances in artificial intelligence, and develop new flood management strategies.

#Freshwater early warning systems

Freshwaters in UK rivers and lakes are being impacted by chemical pollution, disease and biodiversity loss. Research has also shown that these factors can impact human health and aquatic ecosystems.

A CDT led by the University of Bath will train doctoral students to improve real-time monitoring and management of freshwaters. The aim is ultimately to reverse global environmental degradation and better protect both wildlife and human health.

#Wetland wildscapes

A new CDT led by Heriot-Watt University will deliver the next generation of innovative researchers and conservationists needed to protect some of the Earth's most vulnerable and valuable ecosystems.

Wetlands and fringing ecosystems are critical for combating the climate emergency and enhancing community resilience to extreme weather events such as storms and floods. These ecosystems are among the most threatened globally. A new generation of doctoral students will build our understanding of how environmental changes are impacting these crucial habitats.

#Mineral resources

Expertise in the sustainable mineral resources sector is in high demand to support the growth of clean energy technologies and advanced manufacturing.

A new CDT led by the University of Leicester will support researchers across three cohorts. PhD projects will be based around the themes of mineral deposit discovery, efficient resource recovery, environmental sustainability, and strategy for supply chains and the circular economy.

Science, Research and Innovation Minister, Andrew Griffith, said:

“Backing our brightest students to tackle issues as vital as flooding and protecting our water quality is an investment in protecting the landscape of the UK, while defending our planet and the resources we need to deliver us all healthier and more prosperous lives.

“With more than £10m in funding over the coming years it will also help to skill-up students in high-value research, which will grow the UK economy and ensure we fulfil the potential of the talent spread throughout our country.”

Professor Peter Liss, Interim Executive Chair of NERC, said:

“This investment by NERC will equip the next generation of environmental science researchers with the technical and professional skills to tackle some of the most significant challenges facing the UK and globally. 

 

“The new centres for doctoral training will focus on the key themes of flood management, freshwater quality, sustainable mineral resources and wetland conservation.”

 

This investment is the first time NERC has funded four new CDTs in one year, and follows the announcement in November that NERC would rebalance its doctoral provision from 2024.[LINK]

The move also supports the transition to collective talent funding across UK Research and Innovation (UKRI) with the aim of improving the flow of talent across disciplines and sectors in research and innovation.

NERC’s new CDTs will take their first cohort of students from October.

 

Ends

Further information:

Media enquiries:

Sophie Docker, Interim Media and Communications Lead, UKRI

Sophie.docker@ukri.org or 07513 485076

NERC will launch new funding opportunities for doctoral landscape awards in January 2024 and doctoral focal award in February 2024.

Centres for doctoral training project summaries:

Doctoral Training for Resilient Flood Futures (FLOOD-CDT)

Led by Professor Ivan Haigh, University of Southampton

Hosting partners: University of Southampton, University of Bristol, Loughborough University, Newcastle University, National Oceanography Centre, UK Centre for Ecology & Hydrology, British Geological Survey.

FLOOD-CDT is a collaborative venture, made up of world-leading researchers and educators from four universities and three research centres together with 40 partners across the UK. It will develop understanding of: flooding across all flood drivers (fluvial, pluvial, coastal, tsunamis, dam failures and groundwater); how climate change, changing population, development and land use, and public perception affect flood risk; mapping flood dynamics and damage, and improving forecasting; make use of AI in modelling floods.

Centre for Doctoral Training in Real-Time Digital Water-Based Systems for Environmental Health Protection (RED-ALERT CDT)

Led by Professor Barbara Kasprzyk-Hordern, University of Bath

Hosting partners: University of Bath, Bangor University, Cardiff University, University of Exeter, UK Centre for Ecology & Hydrology 

RED-ALERT will train and empower a new generation of leaders to transform how we manage aquatic environmental health via Real-Time Digital Water-Based Systems.

Training will focus on challenge-led multidisciplinary research and problem-solving skills. Leveraging nationally leading research facilities, international networks and whole system "Living Labs" to provide the in-depth knowledge and breadth of understanding, it will enable a step-change in managing environmental health.

Centre for Ecologically Relevant Multiple Stressor Effects on Wetland Wildscapes (ECO-WILD)

Led by Dr Frances Orton, Heriot-Watt University

Hosting partners: Heriot-Watt University, Oxford University, University of York, University of the Highlands and Islands, UK Centre for Ecology & Hydrology. 

ECOWILD is a unique partnership that brings together research expertise and innovation across various fields including environmental toxicology, ecology, multiple stressor theory and modelling, wetland conservation and restoration, socioeconomics, community engagement, management and governance; and involves some of the leading UK experts in these fields. We will address scientific topics including wetland conservation and restoration, climate and pollution impacts, wetland management and creation, nature-based solutions and advances in multi-stressor theory and application.

The ECOWILD CDT will run over six years and comprise three cohorts, each of 13-14 students. During their three year and eight-month PhD, ECOWILD students will work on a challenging research project aligned with one or more of five priority research areas, identified through horizon scanning exercises and in collaboration with our stakeholders.

TARGET Mineral Resources - Training And Research Group for Energy Transition Mineral Resources

Led by Dr Daniel Smith, University of Leicester

Hosting partners: University of Leicester, Cardiff University, University of Exeter, British Geological Survey, Natural History Museum, Imperial College London, SUERC/University of Glasgow, UCL, University of Edinburgh, University of St Andrews, University of Southampton, University of Liverpool, University of Leeds, University of Brighton, University of Aberdeen  

TARGET will train up to 36 multidisciplinary mineral resources researchers across three cohorts. PhD projects will be based around the themes of mineral deposit discovery, efficient resource recovery, environmental sustainability, and strategy for supply chains and the circular economy. Cohort training will deliver multidisciplinary skills and knowledge that cover the whole of the resources lifecycle and prepare researchers for careers in a rapidly changing industry that is vital to the UK's green energy transition. https://target.le.ac.uk/

For Immediate Release

 

UK invests in a new generation of environmental scientists

Four new centres for doctoral training will boost UK expertise in flood management, wetland restoration, sustainable mineral resources and freshwater quality.

The new Natural Environment Research Council-funded centres will teach the next generation of PhD students who will go on to build careers in research, business and public service. Each centre will be supported with £2.6 million funding.

The centres for doctoral training  (CDTs) will focus on following themes:

#Flooding

Millions of homes and businesses are at risk of flooding in the UK from rivers, the sea or surface water, with billions more affected globally.

A new CDT led by the University of Southampton will improve understanding of: drivers of flooding, for example, from rainfall, enhanced river discharge, surface runoff and storm surges; and climate change, changing population and development, and public perceptions affect flood risk.

The new cohort of researchers will develop new approaches to map and forecast floods, including using state-of-the-art monitoring and advances in artificial intelligence, and develop new flood management strategies.

#Freshwater early warning systems

Freshwaters in UK rivers and lakes are being impacted by chemical pollution, disease and biodiversity loss. Research has also shown that these factors can impact human health and aquatic ecosystems.

A CDT led by the University of Bath will train doctoral students to improve real-time monitoring and management of freshwaters. The aim is ultimately to reverse global environmental degradation and better protect both wildlife and human health.

#Wetland wildscapes

A new CDT led by Heriot-Watt University will deliver the next generation of innovative researchers and conservationists needed to protect some of the Earth's most vulnerable and valuable ecosystems.

Wetlands and fringing ecosystems are critical for combating the climate emergency and enhancing community resilience to extreme weather events such as storms and floods. These ecosystems are among the most threatened globally. A new generation of doctoral students will build our understanding of how environmental changes are impacting these crucial habitats.

#Mineral resources

Expertise in the sustainable mineral resources sector is in high demand to support the growth of clean energy technologies and advanced manufacturing.

A new CDT led by the University of Leicester will support researchers across three cohorts. PhD projects will be based around the themes of mineral deposit discovery, efficient resource recovery, environmental sustainability, and strategy for supply chains and the circular economy.

Science, Research and Innovation Minister, Andrew Griffith, said:

“Backing our brightest students to tackle issues as vital as flooding and protecting our water quality is an investment in protecting the landscape of the UK, while defending our planet and the resources we need to deliver us all healthier and more prosperous lives.

“With more than £10m in funding over the coming years it will also help to skill-up students in high-value research, which will grow the UK economy and ensure we fulfil the potential of the talent spread throughout our country.”

Professor Peter Liss, Interim Executive Chair of NERC, said:

“This investment by NERC will equip the next generation of environmental science researchers with the technical and professional skills to tackle some of the most significant challenges facing the UK and globally. 

“The new centres for doctoral training will focus on the key themes of flood management, freshwater quality, sustainable mineral resources and wetland conservation.”

This investment is the first time NERC has funded four new CDTs in one year, and follows the announcement in November that NERC would rebalance its doctoral provision from 2024.[LINK]

The move also supports the transition to collective talent funding across UK Research and Innovation (UKRI) with the aim of improving the flow of talent across disciplines and sectors in research and innovation.

NERC’s new CDTs will take their first cohort of students from October.

Ends

Further information:

Media enquiries:

Sophie Docker, Interim Media and Communications Lead, UKRI

Sophie.docker@ukri.org or 07513 485076

NERC will launch new funding opportunities for doctoral landscape awards in January 2024 and doctoral focal award in February 2024.

Centres for doctoral training project summaries:

Doctoral Training for Resilient Flood Futures (FLOOD-CDT)

Led by Professor Ivan Haigh, University of Southampton

Hosting partners: University of Southampton, University of Bristol, Loughborough University, Newcastle University, National Oceanography Centre, UK Centre for Ecology & Hydrology, British Geological Survey.

FLOOD-CDT is a collaborative venture, made up of world-leading researchers and educators from four universities and three research centres together with 40 partners across the UK. It will develop understanding of: flooding across all flood drivers (fluvial, pluvial, coastal, tsunamis, dam failures and groundwater); how climate change, changing population, development and land use, and public perception affect flood risk; mapping flood dynamics and damage, and improving forecasting; make use of AI in modelling floods.

Centre for Doctoral Training in Real-Time Digital Water-Based Systems for Environmental Health Protection (RED-ALERT CDT)

Led by Professor Barbara Kasprzyk-Hordern, University of Bath

Hosting partners: University of Bath, Bangor University, Cardiff University, University of Exeter, UK Centre for Ecology & Hydrology 

RED-ALERT will train and empower a new generation of leaders to transform how we manage aquatic environmental health via Real-Time Digital Water-Based Systems.

Training will focus on challenge-led multidisciplinary research and problem-solving skills. Leveraging nationally leading research facilities, international networks and whole system "Living Labs" to provide the in-depth knowledge and breadth of understanding, it will enable a step-change in managing environmental health.

Centre for Ecologically Relevant Multiple Stressor Effects on Wetland Wildscapes (ECO-WILD)

Led by Dr Frances Orton, Heriot-Watt University

Hosting partners: Heriot-Watt University, Oxford University, University of York, University of the Highlands and Islands, UK Centre for Ecology & Hydrology. 

ECOWILD is a unique partnership that brings together research expertise and innovation across various fields including environmental toxicology, ecology, multiple stressor theory and modelling, wetland conservation and restoration, socioeconomics, community engagement, management and governance; and involves some of the leading UK experts in these fields. We will address scientific topics including wetland conservation and restoration, climate and pollution impacts, wetland management and creation, nature-based solutions and advances in multi-stressor theory and application.

The ECOWILD CDT will run over six years and comprise three cohorts, each of 13-14 students. During their three year and eight-month PhD, ECOWILD students will work on a challenging research project aligned with one or more of five priority research areas, identified through horizon scanning exercises and in collaboration with our stakeholders.

TARGET Mineral Resources - Training And Research Group for Energy Transition Mineral Resources

Led by Dr Daniel Smith, University of Leicester

Hosting partners: University of Leicester, Cardiff University, University of Exeter, British Geological Survey, Natural History Museum, Imperial College London, SUERC/University of Glasgow, UCL, University of Edinburgh, University of St Andrews, University of Southampton, University of Liverpool, University of Leeds, University of Brighton, University of Aberdeen  

TARGET will train up to 36 multidisciplinary mineral resources researchers across three cohorts. PhD projects will be based around the themes of mineral deposit discovery, efficient resource recovery, environmental sustainability, and strategy for supply chains and the circular economy. Cohort training will deliver multidisciplinary skills and knowledge that cover the whole of the resources lifecycle and prepare researchers for careers in a rapidly changing industry that is vital to the UK's green energy transition. https://target.le.ac.uk/

 

New study reveals significant reduction in lumbar spine reoperation with chiropractic spinal manipulation



Peer-Reviewed Publication

UNIVERSITY HOSPITALS CLEVELAND MEDICAL CENTER





A new study conducted by researchers at University Hospitals (UH) Connor Whole Health sheds light on the potential benefits of chiropractic spinal manipulation (CSM) for adults experiencing ongoing sciatica following low back surgery. The study, titled "Association between spinal manipulative therapy and lumbar spine reoperation after discectomy: a retrospective cohort study," was recently published in BMC Musculoskeletal Disorders.

The researchers investigated whether adults receiving CSM for sciatica at least one year after lumbar discectomy would be less likely to undergo lumbar spine reoperation compared to matched controls not receiving CSM, over a two-year follow-up period. Using a retrospective cohort study design, the research team analyzed data from a United States network of health records (TriNetX, Inc.) spanning from 2003 to 2023. The authors used a statistical technique called propensity matching to make the chiropractic and usual medical care cohorts similar according to age and other features. The study included a total of 756 patients, with a mean age of 61 years.

The study revealed that patients who received chiropractic spinal manipulation experienced a substantial 45% reduction in the likelihood of undergoing additional lumbar surgery compared to those who received usual medical care. Specifically, the proportion of patients undergoing lumbar spine reoperation was 7% in the chiropractic spinal manipulation group, significantly lower than the 13% in the usual medical care group.

Lead author Robert J Trager, DC, noted, "This is the largest study of its kind to date and shows promise for chiropractic spinal manipulation in this population. However, we need to be careful about interpreting the findings, considering it is an observational study."

The researchers called for further research to explore the relevance of their findings, with studies that would include measures of pain, disability, and safety. The study also raises questions about the possibility of a generalized effect of engaging with a non-surgical clinician, prompting comparison of the findings to related contexts such as physical therapy or acupuncture.

Reference:

Trager RJ, Gliedt JA, Labak CM, Daniels CJ, Dusek JA: Association between spinal manipulative therapy and lumbar spine reoperation after discectomy. BMC Musculoskelet Disord. 2024, 25:46. [DOI: 10.1186/s12891-024-07166-x]

https://bmcmusculoskeletdisord.biomedcentral.com/articles/10.1186/s12891-024-07166-x

 EVERYBODY LOVES WATER BEARS

Molecular sensor enables water bear hardiness by triggering dormancy


Free radicals sensor triggers tardigrades to enter dehydrated tun state to withstand extreme stress


Peer-Reviewed Publication

PLOS

Molecular sensor enables water bear hardiness by triggering dormancy 

IMAGE: 

TARDIGRADE OBSERVED USING A CONFOCAL FLUORESCENT MICROSCOPE. THE TARDIGRADE WAS OVEREXPOSED TO 5-MF, A CYSTEINE SELECTIVE FLUORESCENT PROBE, THAT ALLOWS FOR VISUALIZATION OF INTERNAL ORGANS.

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CREDIT: SMYTHERS ET AL., 2024, PLOS ONE, CC-BY 4.0 (HTTPS://CREATIVECOMMONS.ORG/LICENSES/BY/4.0/)






Tardigrades – hardy, microscopic animals commonly known as “water bears” – use a molecular sensor that detects harmful conditions in their environment, telling them when to go dormant and when to resume normal life. A team led by Derrick R. J. Kolling of Marshall University and Leslie M. Hicks of the University of North Carolina at Chapel Hill report these findings in a new study published January 17 in the open-access journal PLOS ONE.

Water bears are famous for their ability to withstand extreme conditions, and can survive freezing, radiation, and environments without oxygen or water. They persist by going dormant and entering a tun state, in which their bodies become dehydrated, their eight legs retract and their metabolism slows to almost undetectable levels. Previously, little was known about what signals water bears to enter and leave this state.

In the new study, researchers exposed water bears to freezing temperatures or high levels of hydrogen peroxide, salt or sugar to trigger dormancy. In response to these harmful conditions, the animals’ cells produced damaging oxygen free radicals. The researchers found that water bears use a molecular sensor—based on the amino acid cysteine—which signals the animals to enter the tun state when it is oxidized by oxygen free radicals. Once conditions improve and the free radicals disappear, the sensor is no longer oxidized, and the water bears emerge from dormancy. When the researchers applied chemicals that block cysteine, the water bears could not detect the free radicals and failed to go dormant.

Altogether, the new results indicate that cysteine is a key sensor for turning dormancy on and off in response to multiple stressors, including freezing temperatures, toxins and concentrated levels of salt or other compounds in the environment. The findings suggest that cysteine oxidation is a vital regulatory mechanism that contributes to water bears’ remarkable hardiness and helps them survive in ever-changing environments.

The authors add: "Our work reveals that tardigrade survival to stress conditions is dependent on reversible cysteine oxidation, through which reactive oxygen species serve as a sensor to enable tardigrades to respond to external changes."

#####

In your coverage please use this URL to provide access to the freely available article in PLOS ONEhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0295062

Citation: Smythers AL, Joseph KM, O’Dell HM, Clark TA, Crislip JR, Flinn BB, et al. (2024) Chemobiosis reveals tardigrade tun formation is dependent on reversible cysteine oxidation. PLoS ONE 19(1): e0295062. https://doi.org/10.1371/journal.pone.0295062

Author Countries: USA

Funding: This research was supported by National Science Foundation grants awarded to L.M.H. (NSF-MCB 2149172) and D.R.J.K. (NSF-MCB 2149173). A.L.S. acknowledges funding from the North Carolina Space Grant. Marshall University students were also funded by a National Science Foundation (NSF) Grant (Award Nos. CHE1229498 and OIA1458952), the NASA West Virginia Space Grant Consortium (Grant no. NNX15AK74A). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 

NIH-developed HIV antibodies protect animals in proof-of-concept study


Findings support the HIV fusion peptide as a promising preventive vaccine target

Peer-Reviewed Publication

NIH/NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

HIV-1 virus particles 

IMAGE: 

TRANSMISSION ELECTRON MICROGRAPH OF HIV-1 VIRUS PARTICLES (BLUE) FROM INFECTED H9 CELLS, PRODUCED IN CELL CULTURE. THE PARTICLES EXHIBIT TWO STAGES OF REPLICATION: THE TWO “ARCS” ARE IMMATURE PARTICLES BUDDING FROM THE PLASMA MEMBRANE OF THE CELL, AND THE CENTER SPHERICAL PARTICLE IS A MATURE FORM IN EXTRACELLULAR SPACE. IMAGE CAPTURED AT THE NIAID INTEGRATED RESEARCH FACILITY IN FORT DETRICK, MARYLAND. 

view more 

CREDIT: NIAID





WHAT:
Three different HIV antibodies each independently protected monkeys from acquiring simian-HIV (SHIV) in a placebo-controlled proof-of-concept study intended to inform development of a preventive HIV vaccine for people. The antibodies—a human broadly neutralizing antibody and two antibodies isolated from previously vaccinated monkeys—target the fusion peptide, a site on an HIV surface protein that helps the virus fuse with and enter cells. The study, published in Science Translational Medicine, was led by the Vaccine Research Center (VRC) at the National Institute of Allergy and Infectious Diseases (NIAID), part of the National Institutes of Health. 

Antibodies that target the fusion peptide can neutralize diverse strains of HIV in vitro, that is, in a test tube or culture dish outside of a living organism. The NIAID VRC isolated a fusion peptide-directed human antibody, called VRC34.01, from a person living with HIV who donated blood samples for research. They also isolated two antibodies from rhesus macaques—a species of monkey with immune systems like humans’—who previously had received a vaccine regimen designed to generate fusion peptide-directed antibodies. Demonstrating that these antibodies protect animals would validate the fusion peptide as a target for human vaccine design. SHIV challenge—administering an infective dose of SHIV—to rhesus macaques is a widely used animal model for assessing the performance of HIV antibodies and vaccines. 

In this study, rhesus macaques in each of four groups received a single intravenous infusion of one type of antibody—a 2.5 or 10 mg/kg of bodyweight dose of VRC34.01, or one of the two vaccine-elicited rhesus macaque antibodies—and other monkeys received a placebo infusion. To determine the protective effect of the antibodies, each monkey was challenged five days after infusion with a strain of SHIV known to be sensitive to fusion peptide-directed antibodies. 

All monkeys that received a placebo infusion acquired SHIV following the challenge. Among monkeys that received VRC34.01 infusions, none receiving the 10 mg/kg dose and 25% of those receiving the 2.5 mg/kg dose acquired SHIV. Of those that received the vaccine-elicited rhesus macaque antibodies, no monkeys receiving the antibody called DFPH-a.15 acquired SHIV, and 25% of those receiving the antibody called DF1W-a.01 acquired SHIV. Over time, the concentration of antibodies in the blood of animals that received DFPH-a.15 declined. Those animals were re-challenged 30 days later to see if the lower concentration of antibodies had a decreased protective effect, and half of them acquired SHIV. 

The three antibodies studied each provided statistically significant protection from SHIV, and the effect was dose dependent, that is, highest in monkeys with greater antibody concentrations in their blood. 

According to the authors, these findings represent the proof-of-concept that fusion peptide-directed antibodies can provide protection against SHIV and help determine the concentration of antibodies a vaccine would need to generate to be protective. They suggest that their findings on vaccine-elicited antibodies in some animals support further work to design preventive HIV vaccine concepts targeting the fusion peptide. They conclude that an effective HIV vaccine targeting the HIV fusion peptide likely will need to expand upon the concepts used in this study, by generating multiple varieties of fusion peptide-directed antibodies. This would increase the likelihood that the vaccine could maintain a preventive effect across the vastly diverse HIV variants in circulation.

ARTICLE:
A Pegu et al. Antibodies targeting the fusion peptide on the HIV envelope provide protection to rhesus macaques against mucosal SHIV challenge. Science Translational Medicine DOI: 10.1126/scitranslmed.adh9039 (2024).

NIAID conducts and supports research—at NIH, throughout the United States, and worldwide—to study the causes of infectious and immune-mediated diseases, and to develop better means of preventing, diagnosing and treating these illnesses. News releases, fact sheets and other NIAID-related materials are available on the NIAID website.

About the National Institutes of Health (NIH): NIH, the nation's medical research agency, includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. NIH is the primary federal agency conducting and supporting basic, clinical, and translational medical research, and is investigating the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit http://www.nih.gov/.

NIH...Turning Discovery Into Health®
 

Chromatin modifier-centered pathway points to higher crop yield


 JAN-2024

CHINESE ACADEMY OF SCIENCES HEADQUARTERS
TGW3 phosphorylation of HHC4 shapes rice grain size through functional impairment of a chromatin modifier ternary complex 

IMAGE: 

TGW3 PHOSPHORYLATION OF HHC4 SHAPES RICE GRAIN SIZE THROUGH FUNCTIONAL IMPAIRMENT OF A CHROMATIN MODIFIER TERNARY COMPLEX

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CREDIT: IBCAS





Chromatin is the complex of DNA and proteins that makes up the genetic material in the nucleus of eukaryotic cells. A chromatin modifier is a protein or complex of proteins that chemically modifies the structure of chromatin. Chromatin modifiers play a crucial role in regulating the expression of genes, which are segments of DNA strands, as well as in other chromatin-related processes. These modifiers mainly work by adding or subtracting chemical groups to histones, a type of protein within the chromatin, or to the DNA itself.

In the scientific effort to manipulate the expression of plant genes, such as for grain size or drought-resistance, etc., understanding the influence of chromatin modifiers is an important avenue of research.

A team led by Prof. SONG Xianjun from the Institute of Botany of the Chinese Academy of Sciences, while researching a ternary protein complex in rice nuclei that affects grain size, has shown that the transcription factor bZIP23—a protein that regulates the transcription of genetic DNA information into RNA and is part of the ternary complex—recruits the chromatin-modifying histone acetyltransferase HHC4 to specific promoters on the DNA. Furthermore, they discovered that HHC4 and the adaptor protein ADA2 additively enhance the bZIP23 transactivation of target genes.

The researchers also showed that HHC4 is phosphorylated by the GSK3-like kinase TGW3, which triggers a series of negative influences on the capabilities of the ternary complex.

These findings, published in Developmental Cell, contribute to a deeper understanding of the epigenetic regulation of grain size.

A previous study by Prof. SONG showed that GRAIN WEIGHT 6a (GW6a) encodes a newly identified histone acetyltransferase (OsglHAT1) that is a positive regulator of grain size and rice yield.

“At the beginning of this study, we identified five rice homologs of OsglHAT1 on chromosomes 2, 3, 4, and 7 (hence named HHCs) and sought to investigate whether these homologs also modulate grain size,” said Dr. SHEN Shaoyan, first author of the study. "Interestingly, HHC4 regulates grain size but adopts a different cytological mechanism from GW6a."

Using a series of molecular biology techniques, including chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) and immunoprecipitation followed by mass spectrometry (IP-MS), bZIP23 was shown to directly interact with HHC4.

The bZIP23 gene has been characterized for its role in salinity and drought resistance as well as seed vigor in rice; however, whether it could regulate grain size was unknown. The researchers subsequently discovered that overexpression of bZIP23 significantly increased rice grain size, and bZIP23 and HHC4 co-targeted and synergistically activated the expression of several positive regulators of grain size.

Furthermore, the researchers found that HHC4, ADA2, and bZIP23 interact with each other, and the resulting ternary complex facilitates the additive enhancement of bZIP23 transactivation on target genes by ADA2 and HHC4.

Meanwhile, yeast two-hybrid (Y2H) screening revealed that the GSK3-like kinase protein TGW3 also interacts with HHC4. In addition, the researchers showed that HHC4 is directly phosphorylated by TGW3.

Mutation analysis suggested that two serine residues (S189 and S190) of HHC4 are major sites of phosphorylation by TGW3.

Comparisons with mature grains of transgenic rice plants containing the mimicked phosphorylated and unphosphorylated versions of HHC4 suggested that phosphorylation is involved in grain size control.

Subsequent experimental data showed that phosphorylation exerts many negative influences, such as on the protein stability of HHC4, its interaction with bZIP23, and bZIP23's transactivation of target genes.

In addition, the researchers showed that both HHC4 overexpression and TGW3 knockout substantially increased rice grain yield by up to 24% in field trials.

Overall, these findings uncover a previously unknown chromatin modifier-centered pathway for grain size regulation in rice and provide useful genetic resources for high-yield crop breeding programs.

CRIMINAL CAPITALI$M;BIG PHARMA

Panel members for new psychiatric ‘bible’ received over $14M from industry


Study finds six in 10 US physician contributors had financial ties to industry. Findings raise questions about editorial independence

BMJ

Sixty percent of US physicians serving as panel and task force members for the American Psychiatric Association’s official manual of psychiatric disorders received payments from industry totalling $14.24m, finds a study published by The BMJ.

Because of the enormous influence of diagnostic and treatment guidelines, the researchers say their findings “raise questions about the editorial independence of this diagnostic manual.”

Often referred to as the ‘bible’ of psychiatric disorders, the Diagnostic and Statistical Manual of Mental Disorders, fifth edition, text revision (DSM-5-TR) is the latest edition of the guide that doctors use to diagnose and treat patients. It is thus critical that authors of this psychiatric taxonomy should be free of industry ties.

But until the development of Open Payments (a database of financial relationships between companies and physicians), it wasn’t possible to determine the amount of monies received by authors of diagnostic and clinical practice guidelines.

To address this, researchers used data from Open Payments to assess the extent and types of financial ties to industry of panel and task force members of the DSM-5-TR.

Their analysis included 92 physicians based in the US who served as members of either a panel (86) or task force (6) on the DSM-5-TR from 2016-19, the time during which work was initiated and completed for the 2022 text revision.

Of these 92 individuals, 55 (60%) received payments from industry. Collectively, these panel members received a total of $14.24m (£11.21m; €12.96m). Only two of the six task force members had any payments reported in Open Payments, totaling $196.02 and $792.67 for 2016-19.

The most common types of payment were for food and beverages (91%), followed by travel (69%) and consulting (69%).

The greatest proportion of compensation by category of payment was for research funding (70%) which the authors point out was excluded from the American Psychiatric Association’s disclosure policy for the previous edition (DSM-5).

They highlight some study limitations, such as not including payments to physicians based outside the US or non-physician prescribers and acknowledge that amounts listed in the database may be imprecise.

Nevertheless, they say this study “provides novel data about the appreciable conflicts of interest in the DSM-5-TR and extends past research on this topic.”

To ensure unbiased, evidence based mental health practice, there should be a rebuttable presumption of prohibiting financial conflicts of interest among the panel and task force members of the Diagnostic and Statistical Manual of Mental Disorders, they write.

When no independent individuals with the requisite expertise are available, they suggest that those with associations to industry could consult to the panels, but they would not have decision making authority on revisions or inclusion of new disorders.

“As researchers, clinicians, policy makers, and leaders in evidence based medicine have argued, guideline writers should be free of financial relationships with industry, especially those writers who are responsible for such an influential manual on psychiatric taxonomy,” they conclude.