Thursday, June 05, 2025

 

Archaeologists uncover massive 1000-year-old Native American fields in Northern Michigan that defy limits of farming




Dartmouth College
Raised agricultural beds at Sixty Islands archaeological site along the Menominee River. 

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Raised agricultural beds cover an estimated 70% of the lidar survey area at Sixty Islands archaeological site along the Menominee River.

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Credit: Photo by Madeleine McLeester.





With its cold climate, short growing season, and dense forests, Michigan's Upper Peninsula is known as a challenging place for farming. But a new Dartmouth-led study provides evidence of intensive farming by ancestral Native Americans at the Sixty Islands archaeological site along the Menominee River, making it the most complete ancient agricultural site in the eastern half of the United States.

The site features a raised ridge field system that dates to around the 10th century to 1600, and much of it is still intact today.

The raised fields are comprised of clustered ridged garden beds that range from 4 to 12 inches in height and were used to grow corn, beans, squash, and other plants by ancestors of the Menominee Indian Tribe of Wisconsin.

The findings are published in Science.

"The scale of this agricultural system by ancestral Menominee communities is 10 times larger than what was previously estimated," says lead author Madeleine McLeester, an assistant professor of anthropology at Dartmouth. “That forces us to reconsider a number of preconceived ideas we have about agriculture not only in the region, but globally.”

The researchers surveyed approximately 330 acres. However, they have yet to map the entire site because it extends beyond the edge of where they surveyed. They estimate that they surveyed approximately 40% percent of the site. 

"When you look at the scale of farming, this would require the kind of labor organization that is typically associated with a much larger, state-level hierarchical society," says McLeester. "Yet, everything we know about this area suggests smaller egalitarian societies lived in this region but in fact, this may have been a rather large settlement."

The site is part of Anaem Omot, which stands for the "Dog's Belly" in Menominee. Anaem Omot is a cluster of significant ancestral Menominee archaeological sites that includes several burial mounds and a village that were excavated from the 1950s through the 1970s. It was initially mapped in the 1990s by Marla Buckmaster, an archaeologist at Northern Michigan University, and excavated by Jan Brashler, an archaeologist at Grand Valley State University, who found and radiocarbon dated a corn cupule (the cup-shaped structure on the cob that holds a kernel in place) during excavations. Given the site's cultural significance with its burial mounds, dance rings, and agricultural ridges, it is listed on the U.S. National Register of Historic Places.

Building on earlier work and upon the request of the Menominee tribal authorities, archaeologists from Dartmouth were invited to survey and document the area using new technologies that were previously unavailable. Through this partnership, the team collaborated with David Grignon, tribal historic preservation officer for the Menominee Indian Tribe of Wisconsin and the late David Overstreet, a consulting archaeologist for the College of the Menominee Nation.

In May 2023, after the snow had melted but before the leaves were out, the team conducted an innovative, drone-based survey of a 330-acre area using lidar, a remote sensing technology that uses pulses of light from a laser to map objects on the Earth's surface. Lidar provides a dataset that's like a giant cloud of points with the locations of the trees and rocks, which can be filtered out to see the ground. 

"Lidar is a really powerful tool in any kind of forested or heavily vegetated region where the archaeology is hidden below trees—where no kind of optical imagery can see what's underneath the tree canopy," says senior author Jesse Casana, a professor of anthropology who uses remote sensing technologies regularly in his work.

"Forests are really confounding to archaeology in a lot of ways, so a lot of archaeologists rely on publicly available lidar that has often been obtained from a plane that flies really high. But the resolution of the data is usually too low to see many archaeological features. Drone lidar enables us to collect the same kind of data but at a much higher resolution," says Casana.

The lidar uncovered sets of parallel ridges at the site that create quilt-like patterns stretched across the landscape. The ridges were constructed in various directions, illustrating that their locations may have been determined by individual farmers rather than the direction of the sun or other environmental factors.

The results also revealed: a circular dance ring, a rectangular building foundation that may have been a colonial trading post, two 19th-century logging camps, looted burial mounds, previously unknown burial mounds that were thought to be destroyed in the 1970s, and a burial mound on privately owned land that is currently owned by a mining company.

In August 2023, the team excavated three raised agricultural ridges at various distances from the Menominee River. Through radiocarbon dating of charcoal samples obtained during excavation, they found that the ridges had been rebuilt over a 600-year period, with construction initially around the year 1000, which was during the Late Woodland period.

"All three ridges showed a similar picture in terms of their construction, history and reconstruction," says Casana. "Most field systems have been either lost or destroyed due to intensive land use across most of North America, through farming, including pastures, and the cutting down of trees for urban development."

"Through this research, we get this little window of preservation into pre-Colonial farming in the Upper Peninsula of Michigan," says Casana.

Through the excavations, charcoal, broken pieces of ceramics known as sherds, and artifacts were recovered, suggesting that remains from fires and household refuse were likely used as compost in the fields. The results also showed that wetland soils had been used to enrich the soil. 

"Our work shows that the ancestral Menominee communities were modifying the soil to completely rework the topography in order to plant and harvest corn at the near northern extent of where this crop can grow," says McLeester. "This farming system was a massive undertaking requiring a lot of organization, labor, and know-how to maximize agricultural productivity." 

"We're seeing this kind of landscape alteration in a place where we wouldn't expect it," says McLeester.

"This may be just a little remnant of what must have been a much larger system," says Casana.

The findings have made the researchers consider if perhaps the majority of eastern North America was once covered with agricultural ridges. Findings also challenge existing forest history of the Upper Peninsula, since Sixty Islands would have been deforested during this 600 year period.

The team is continuing their work with the Menominee Indian Tribe of Wisconsin at the Menominee Sixty Islands site with upcoming plans to survey the site and locate ancestral Menominee villages.

McLeester (madeleine.mcleester@dartmouth.edu) and Casana (jesse.j.casana@dartmouth.edu) are available for comment.

Carolin Ferwerda, a research scientist in the Department of Anthropology and Jonathan Alperstein, Guarini, a graduate student in the Ecology, Evolution, Environment & Society program in the Department of Anthropology at Dartmouth also contributed to the study.

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Map shows Sixty Islands and other archaeological sites along the Menominee River, and the lidar survey area.

Credit

Map by Carolin Ferwerda.

Lidar data detected other cultural features at Sixty Islands archaeological site in addition to agricultural field ridges, including: A) a newly documented dance ring; B) a historic building foundation; C) a 19th century logging camp; D) looted burial mounds; E) remains of previously unknown burial mounds at Backlund mound group; and F) a burial mound.

Credit

Lidar images by Carolin Ferwerda and Jesse Casana.

 

Advance in creating organoids could aid research, lead to treatment



Advance in organoid creation



Stanford Medicine

Heart organoid 

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This 2-week-old heart organoid — with cardiomyocytes (green) and smooth muscle cells (white) — is surrounded by endothelial cells (magenta) that form a network of realistic blood vessels.

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Credit: Oscar Abilez/Stanford Medicine





For over a decade, scientists have been growing organoids — small clusters of cells that mimic a particular organ — to serve as miniature biological models. Organoids of the brain have been used to study neurodevelopmental disorders; intestinal organoids, to model celiac disease; and lung organoids, to investigate SARS-CoV-2. Heart organoids have even been sent to space to test the effect of microgravity on cardiac muscle. But there’s a tiny problem — the organoids can’t grow any bigger than a sesame seed.

Unlike living tissue in the body, organoids lack a blood vessel system that delivers oxygen and nutrients to every cell. Beyond about 3 millimeters in diameter, an organoid can no longer sustain itself by absorbing resources directly from its environment.

“When you grow organoids to a certain size they start to die inside because they can’t get oxygen and nutrients to the center,” said Oscar Abilez, MD, PhD, a senior scientist in the Division of Pediatric Cardiac Surgery.

But for a study published June 5 in Science, Abilez and a team of Stanford Medicine researchers grew heart and liver organoids replete with tiny blood vessels, potentially allowing them to overcome the current size limit.

The ability to grow vascularized organoids overcomes a major bottleneck in the field, said Abilez, who is a co-lead author of the study. The integrated blood vessels could allow the organoids to not only grow larger, but also to reach a more mature state, making them more useful as biological models.

Huaxiao (Adam) Yang, a former instructor at the Stanford Cardiovascular Institute and now an assistant professor in biomedical engineering at the University of North Texas, co-led the study.

They could also be the next step in regenerative therapies, said Joseph Wu, MD, PhD, the study’s senior author. Wu is a professor of medicine and of radiology, the director of the Stanford Cardiovascular Institute and the Simon H. Stertzer, MD, Professor.

In a separate clinical study led by Wu, Stanford Medicine researchers are injecting lab-grown heart muscle cells, called cardiomyocytes, into patients with heart dysfunction. “But actual heart tissue contains more than cardiomyocytes,” Wu said. “There are endothelial cells that line blood vessels, smooth muscle cells that surround blood vessels, pericytes that connect blood vessels, fibroblasts and other cells.” 

In the future, perhaps vascularized cardiac organoids grown from a patient’s own stem cells could be surgically implanted to replace lost or damaged tissue.

“The thought is that if organoids have a vascular system, they could connect with the host vasculature, and that’ll give them a better chance to survive,” Abilez said.

Recipe testing

Scientists grow organoids from pluripotent stem cells by bathing the cells in various chemicals — growth factors and other small molecules — to induce their transformation into different cell types.

But attempts to grow vascularized cardiac organoids have produced inconsistent levels of the cell types needed to form blood vessels. Other researchers have tried an engineering approach, separately growing endothelial cells, or even 3D bioprinting vascularized networks, then combining them with a cardiac organoid. But none have achieved organoids with realistic blood vessel systems.

“They don’t really make branched vessels with passageways,” Abilez said.

In the recently published study, the team set out to optimize a chemical recipe to grow heart organoids that could reliably generate nearly all the cell types in the human heart, including cells that form a robust network of blood vessels.

The researchers reviewed the established methods for creating three key types of cells: cardiomyocytes, endothelial cells and smooth muscle cells. They combined these methods into 34 different recipes, or growing conditions — specifying which growth factors, how much and when to add them — for creating cardiac organoids containing all three cell types.

They also modified stem cells to fluoresce in different colors when they transformed into the three cell types.

When they tested the 34 recipes on stem cells and allowed them to grow for about two weeks, one in particular — condition 32 — was the clear winner. It produced the most colorful cardiac organoid.

“It was pretty obvious,” Abilez said. “We picked the one that gave us the most amount of those three fluorescent colors, which correspond to the most cardiomyocytes, endothelial cells and smooth muscles cells.”

Under 3D microscopy, the doughnut-shaped organoids were organized with cardiomyocytes and smooth muscle cells on the inside, along with an outer layer of endothelial cells that formed unmistakable blood vessels. These tiny branching, tubular vessels resemble the capillaries in the heart, which are 10 to 100 microns, about a hair’s width, in diameter.

When the researchers analyzed the other cells in the organoids using single-cell RNA sequencing, they were surprised to find nearly all the other cell types of the heart. Each organoid contained 15 to 17 different cell types, comparable to a six-week-old embryonic heart, which has 16 cell types. An adult heart has 21 cell types. 

“It had all these other cell types that are found in the heart,” Abilez said. “That was unexpected in a positive way.”

Model of development

The winning recipe seems to approximate the conditions found in early stages of embryonic development, when different cell types emerge and blood vessels begin to form.

That suggests the organoids could be valuable as models of the earliest stages of human development, a period that is difficult to study.

“There’s this black box of development in early pregnancy when it’s not possible, ethically, to test drugs,” Abilez said. 

As proof of concept, the researchers tested fentanyl, a potent and often misused opioid, on the vascularized cardiac organoids. They found that organoids exposed to fentanyl generated more blood vessels.

“We don’t know how that might manifest in a newborn yet, but it’s a difference,” Abilez said.

Other organs

The researchers also showed that their vascularization strategy could be adapted to create other organoids. By combining established methods to differentiate the key cell types in the liver, they created liver organoids with robust networks of blood vessels.

In future studies, the researchers will allow the vascularized organoids to develop longer to see how large and mature they become. They also plan to further optimize their vascularization recipes to generate even more cell types in the organoids, like immune cells and blood cells, to more closely resemble the makeup of an adult heart and better model adult diseases, Wu said. 

“I’d love to be able to do this in all the different organoid types,” Abilez said. “After all, almost every organ in our body has a blood vessel system.”

Researchers from the University of North Texas, Rosebud Biosciences, Bullseye Biotechnologies and Greenstone Biosciences contributed to the work.

The study was supported by funding from the National Institutes of Health (grants K01HL130608, L30HL138771, R15HD108720, R56HL174856, T32GM136501, K08HL119251, R01HL150693, R01HL141371, R01HL146690, R01HL145676, P01HL141084, R01HL150414, R01HL139679 and K99HL166693), the American Heart Association, Tobacco-Related Disease Research Program, California Institute for Regenerative Medicine, Stanford Maternal and Child Health Research Institute Transdisciplinary Initiatives Program, Stanford Cardiovascular Institute, and Stanford Bio-X Program.

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About Stanford Medicine

Stanford Medicine is an integrated academic health system comprising the Stanford School of Medicine and adult and pediatric health care delivery systems. Together, they harness the full potential of biomedicine through collaborative research, education and clinical care for patients. For more information, please visit med.stanford.edu.

 

Drought-resilient plant holds promise for future food production, study finds




Colorado State University
Sean Gleason scanning plant 

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USDA Agricultural Research Service scientist Sean Gleason uses a micro-CT scanner at a Colorado State University lab to study plants. The machine allows Gleason and his CSU collaborators to view internal plant processes non-destructively. Photo by John Eisele/CSU

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Credit: John Eisele/Colorado State University





For the first time, researchers have demonstrated in an intact plant a long-contested process that allows some plants to rebound from extended drought. The team of Colorado State University, University of Colorado and U.S. Department of Agriculture scientists says understanding this special trait could improve agricultural productivity and food security.  

Drought costs the United States billions in agricultural losses and increased irrigation. Lost productivity lowers food availability and raises prices for consumers.   

When a plant dries out, its water transport system becomes impaired through the formation of gas bubble blockages, or embolisms, in the plant’s water-transporting tissues – xylem. Depending on the extent of embolism, some plants never recover.  

To recover, the gas bubbles must be removed and water flow restored through a process called “refilling.” Plant scientists have been divided on whether refilling happens. Most evidence in favor of refilling has been based on destructive study methods, where a plant is cut and water is forced into tissue at a higher pressure than exists in nature.  

The CSU, CU and USDA researchers said that cutting into plants creates “artifacts,” or byproducts from the study method, which in this case could cause embolisms and lead to inaccurate results. Instead, they used a micro-CT scanner, a specialized X-ray machine, to observe internal plant processes as they happen in nature.  

Their study, published on the cover of April’s edition of Proceedings of the National Academy of Sciences, found a complete reversal of embolism and full recovery in a type of wild grass within 24 hours of watering. 

“This is the first convincing evidence of the reversal, or refilling, of embolism in a vascular plant species, with the plant regaining full functional recovery afterward,” said Sean Gleason, a researcher with the USDA Agricultural Research Service, CSU affiliate and co-author of the paper.  

The researchers have started to look for other plants with this trait to identify the genetic mechanism behind it. Once the mechanism is identified, it could potentially be bred into crops, making them more resilient to drought.  

"If a plant can recover from drought quickly by refilling, then you might be able to recoup some losses during a drought year,” said co-author Troy Ocheltree, a CSU associate professor in the Warner College of Natural Resources. “If plants can refill, this may allow flexibility in the amount and timing of irrigation, although additional work is required to identify how refilling would impact crop water use.” 

Happenstance study subject 

Embolism occurs in all vascular plants, and previous studies found that embolism can’t be reversed in some plants, although they may continue to function at reduced capacity once water is restored.   

For this study, lead author Jared Stewart, a postdoctoral researcher with CSU, CU and the USDA ARS, then-CSU M.S. student Brendan Allen and then-CU Ph.D. student Stephanie Polutchko examined a grass that had been growing in a parking lot to see if it would be a good candidate for their experiment. The grass was thriving in the cracks of the hot, dry asphalt lot, so they figured it might be resilient after drought. 

Sure enough, despite its dead appearance and as much as 88% embolized xylem tissue after a prolonged period without water, the grass’s water-transporting system bounced back literally overnight. 

So far, this is the only species known to refill, but the researchers think there likely are others with this trait.  

“We don't know how common this is,” Ocheltree said. “But the fact that we found a plant in the parking lot that refills makes me think there's probably others out there that also refill. It shifts our mindset.”  

Key partnership and equipment 

The study was facilitated by a partnership with CSU’s College of Veterinary Medicine and Biomedical Sciences, which has a micro-CT scanner for small animals. This specialized machine emits less radiation than many CT scanners, enabling repeated scans without harming subjects.  

Gleason said support from lab staff and the principal investigator, Professor Nicole Ehrhart, director of CSU’s Center for Healthy Aging and Laboratory of Comparative Musculoskeletal Oncology and Traumatology, was essential for the study. Laura Chubb, a lab technician, helped the team by scanning plants initially and then trained Gleason to use the machine.  

“The resilience of this humble grass – reviving its vascular system overnight – was both surprising and deeply compelling,” Ehrhart said. “Collaborations like this remind us how powerful it can be when tools developed for biomedical research are applied in new ways to answer fundamental questions about life. We were thrilled to contribute to such impactful science.”  

“Dr. Ehrhart and Laura made this study possible with their generous offer to help us with our scans and allow us use of their micro-CT lab,” Gleason said. “Without them, we wouldn’t have been able to do this research.” 

For more information, read the USDA ARS press release

 

To spot toxic speech online, try AI



A new tool helps balance accuracy with fairness toward all groups in social media



University of Texas at Austin





Earlier this year, Facebook rolled back rules against some hate speech and abuse. Along with changes at X (formerly Twitter) that followed its purchase by Elon Musk, the shifts make it harder for social media users to avoid encountering toxic speech.

That doesn’t mean that social networks and other online spaces have given up on the massive challenge of moderating content to protect users. One novel approach relies on artificial intelligence. AI screening tools can analyze content on large scales while sparing human screeners the trauma of constant exposure to toxic speech.

But AI content moderation faces a challenge, says Maria De-Arteaga, assistant professor of information, risk, and operations management at Texas McCombs: being fair as well as being accurate. An algorithm may be accurate at detecting toxic speech overall, but it may not detect it equally well across all groups of people and all social contexts.

“If I just look at overall performance, I may say, oh, this model is performing really well, even though it may always be giving me the wrong answer for a small group,” she says. For example, it might better detect speech that’s offensive to one ethnic group than to another.

In new research, De-Arteaga and her co-authors show it’s possible to achieve high levels of both accuracy and fairness. What’s more, they devise an algorithm that helps stakeholders balance both, finding desirable combinations of accuracy and fairness for their particular situations.

With professor Matthew Lease and graduate students Soumyajit Gupta and Anubrata Das of UT’s School of Information, as well as Venelin Kovatchev of the University of Birmingham, United Kingdom, De-Arteaga worked with datasets of social media posts already rated “toxic” and “nontoxic” or safe by previous researchers. The sets totaled 114,000 posts.

The researchers used a fairness measurement called Group Accuracy Parity (GAP), along with formulas that helped train a machine learning model to balance fairness with accuracy. Applying their approach through AI to analyze the datasets:

  • It performed up to 1.5% better than the next-best approaches for treating all groups fairly.
  • It performed the best at maximizing both fairness and accuracy at the same time.

But GAP is not a one-size-fits-all solution for fairness, De-Arteaga notes. Different measures of fairness may be relevant for different stakeholders. The kinds of data needed to train the systems depends partly on the specific groups and contexts for which they’re being applied.

For example, different groups may have different opinions on what speech is toxic. In addition, standards on toxic speech can evolve over time.

Getting such nuances wrong could wrongly remove someone from a social space by mislabeling nontoxic speech as toxic. At the other extreme, missteps could expose more people to hateful speech.

The challenge is compounded for platforms like Facebook and X, which have global presences and serve wide spectrums of users.

“How do you incorporate fairness considerations in the design of the data and the algorithm in a way that is not just centered on what is relevant in the U.S.?” De-Arteaga says.

For that reason, the algorithms may require continual updating, and designers may need to adapt them to the circumstances and kinds of content they’re moderating, she says. To facilitate that, the researchers have made GAP’s code publicly available.

High levels of both fairness and accuracy are achievable, De-Arteaga says, if designers pay attention to both technical and cultural contexts.

“You need to care, and you need to have knowledge that is interdisciplinary,” she says. “You really need to take those considerations into account.”

Finding Pareto Trade-Offs in Fair and Accurate Detection of Toxic Speech” is published in Information Research.

 

Sharp-tailed grouse in south-central Wyoming potentially a distinct subspecies




University of Wyoming
Sharp-tailed grouse 

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A sharp-tailed grouse from the population in south-central Wyoming shows its colors. New research by University of Wyoming scientists finds that the birds in this population are likely a distinct subspecies from Columbian and plains sharp-tailed grouse.

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Credit: Jonathan Lautenbach





For decades, a population of grouse in south-central Wyoming and northwest Colorado has been identified as Columbian sharp-tailed grouse, the same subspecies that can be found in far western Wyoming near Jackson along with Idaho, northern Utah and parts of the Pacific Northwest.

But new research led by University of Wyoming scientists has found that the 8,000-10,000 sharp-tailed grouse found in the shrublands and high deserts of southern Carbon County and northwest Colorado are not Columbian sharp-tailed grouse. Nor are they more closely related to plains sharp-tailed grouse -- a subspecies found in portions of the northern Great Plains in the United States and Canada, including eastern Wyoming -- as was suggested by other researchers in 2006.

Rather, the birds potentially represent a distinct subspecies of sharp-tailed grouse that has been isolated from other populations of the bird for many years. The discovery could have major implications for wildlife managers in Wyoming and other states in the Intermountain West and Pacific Northwest.

“Our results may potentially change the current understanding of sharp-tailed grouse subspecies in western North America, which can impact how to manage them,” wrote the researchers, led by recent UW Ph.D. graduate Jonathan Lautenbach and Professor Jeff Beck, of UW’s Department of Ecosystem Science and Management. Their research appears in the journal Ecology and Evolution.

Currently, there are six recognized subspecies of sharp-tailed grouse in North America, generally separated geographically and by the habitats they occupy. They’re closely related to greater and lesser prairie chickens, which inhabit portions of the grasslands and shrublands of the Great Plains. In Wyoming, plains sharp-tailed grouse can be found in the grasslands of the eastern part of the state.

The sharp-tailed grouse found in Wyoming’s southern Carbon County and northwest Colorado -- which the UW researchers now suggest could be a distinct subspecies -- are isolated from the plains sharp-tailed grouse to the east, as well as from the Columbian sharp-tailed grouse farther west. But the isolated group has been categorized and managed as Columbian sharp-tailed grouse for many years.

The UW researchers used multiple lines of evidence to show that the south-central Wyoming sharp-tailed grouse are neither Columbian sharp-tailed grouse nor plains sharp-tailed grouse. They examined habitat characteristics, the birds’ appearance and two types of genetic data -- combined with computer modeling -- to reach their conclusion.

“Across all four datasets and both modeling techniques, we found that each population (Columbian, plains and south-central Wyoming sharp-tailed grouse) generally represented its own cluster,” the scientists wrote. “Our results suggest that the population of sharp-tailed grouse in south-central Wyoming is different from both Columbian and plains sharp-tailed grouse.”

Among the conservation and management implications of these new findings is a potential 10 percent to 20 percent decrease in the total number of birds recognized as Columbian sharp-tailed grouse, whose population has been estimated to be between 41,000-62,000 across multiple states and British Columbia. Due to habitat fragmentation and declining numbers, the Columbian sharp-tailed grouse has been petitioned for listing under the Endangered Species Act.

Additionally, the researchers say any efforts to boost Columbian sharp-tailed grouse numbers in states including Nevada, Oregon and Washington should not involve translocating sharp-tailed grouse from the isolated population of sharp-tailed grouse in south-central Wyoming and northwest Colorado. In addition to concerns about maintaining genetic integrity, the scientists note there are habitat differences between the two populations.

“Currently, habitat management actions are applied uniformly between Columbian sharp-tailed grouse and populations of sharp-tailed grouse in south-central Wyoming and northwest Colorado,” the researchers wrote. “Our results suggest a need to reevaluate habitat management approaches for sharp-tailed grouse across the range of these species/subspecies in Wyoming, Idaho and northwest Colorado.”

The research was funded, in part, by the Wyoming Game and Fish Department, the U.S. Forest Service, the Bureau of Land Management, the U.S. Department of Agriculture, the Wyoming Wildlife Federation and the High Plains Chapter of Pheasants Forever. Other members of the research team were from the University of North Texas, Boise State University, the Washington Department of Fish and Wildlife, and the George Miksch Sutton Avian Research Center in Bartlesville, Okla.