Saturday, August 10, 2024

 

A vaping cessation text message program for adolescent e-cigarette users


JAMA Network





About The Study: A tailored, interactive text message intervention increased self-reported vaping cessation rates among adolescents recruited via social media channels.

Quote from corresponding author Amanda L. Graham, PhD:

“Health care providers, teachers, and parents have been asking how to help teens quit vaping. This study is a critical breakthrough that demonstrates the power of a behavioral intervention for vaping cessation. Text messages serve as powerful reminders of an initial commitment to quit and can deliver proven behavior change support right to a young person’s phone.

“We also did not see evidence that teens who quit vaping transitioned to smoking. The intervention was effective in reducing dual use (smoking and vaping) and keeping teens in this study from starting to smoke.”

Contact information for Amanda L. Graham, PhD: email Megan Kelley (mkelley@truthinitiative.org).

To access the embargoed study: Visit our For The Media website at this link https://media.jamanetwork.com/ 

(doi:10.1001/jama.2024.11057)

Editor’s Note: Please see the article for additional information, including other authors, author contributions and affiliations, conflict of interest and financial disclosures, and funding and support.

#  #  #

Embed this link to provide your readers free access to the full-text article This link will be live at the embargo time https://jamanetwork.com/journals/jama/fullarticle/10.1001/jama.2024.11057?guestAccessKey=52355ac8-73a4-4e2b-b400-3cf946de795c&utm_source=For_The_Media&utm_medium=referral&utm_campaign=ftm_links&utm_content=tfl&utm_term=080724


 

Neighborhood socioeconomic disadvantage across the life course and premature mortality



JAMA Network




About The Study: 

Low neighborhood socioeconomic status was associated with premature mortality in this study. The risk of premature mortality was greatest among individuals experiencing persistently low neighborhood socioeconomic status from young to middle adulthood. Place-based interventions that target neighborhood social determinants of health should be designed from a life course perspective that accounts for early-life socioeconomic inequality. 

Corresponding Author: To contact the corresponding author, Wayne R. Lawrence, DrPH, email wayne.lawrence@nih.gov.

To access the embargoed study: Visit our For The Media website at this link https://media.jamanetwork.com/

(doi:10.1001/jamanetworkopen.2024.26243)

Editor’s Note: Please see the article for additional information, including other authors, author contributions and affiliations, conflict of interest and financial disclosures, and funding and support.

#  #  #

Embed this link to provide your readers free access to the full-text article This link will be live at the embargo time http://jamanetwork.com/journals/jamanetworkopen/fullarticle/10.1001/jamanetworkopen.2024.26243?utm_source=For_The_Media&utm_medium=referral&utm_campaign=ftm_links&utm_term=080724

About JAMA Network Open: JAMA Network Open is an online-only open access general medical journal from the JAMA Network. On weekdays, the journal publishes peer-reviewed clinical research and commentary in more than 40 medical and health subject areas. Every article is free online from the day of publication. 

 

Cocaine discovery could pave way for treatment for substance abuse



University of Copenhagen - The Faculty of Health and Medical Sciences




You have probably heard of dopamine. The substance also known as the “feel-good hormone”.

Drugs such as cocaine cause a surge of dopamine in the brain. Normally, a protein in the brain called the dopamine transporter (DAT) helps regulate dopamine levels and prevent the brain from thinking that every experience is pleasurable.

However, when affected by cocaine, the brain is unable to regulate dopamine levels. Previously, researchers did not know how cocaine affects the different transporters in the brain, but a new study from the University of Copenhagen has changed that.

“We have learned how cocaine binds to the dopamine transporter, which is a protein responsible for regulating dopamine levels in the brain. What we did was describe the structure of the dopamine transporter, which gave us molecular-level insight into how it is inhibited by cocaine,” says PhD Student Jeppe Cederholm Nielsen, who is one of the researchers responsible for the new study.

Our dream is to find a medical treatment for cocaine abuse.

Professor Claus Løland

When the brain is unable to regulate dopamine levels, everything we do seems great, the researchers explain. Cocaine does not make the brain release more dopamine, but it prevents it from regulating and removing dopamine.

“When we experience something as pleasurable, it is because the dopamine that is released in the brain stimulates the reward centre. The role of the dopamine transporter is to stop this process by removing dopamine,” says Professor Claus Løland from the Department of Neuroscience at the University of Copenhagen and adds:

“When cocaine blocks the dopamine transporter, dopamine will continue to stimulate the reward centre – even though the experience itself is not particularly pleasurable. In other words, we become incapable of distinguishing between what is truly pleasurable and what is not, and then all types of input given by our senses will seem great. You could say that cocaine tricks the brain. It is a form of chemical brainwashing.”

World’s most powerful microscope provides answers

To understand what cocaine does to the brain, the researchers studied the dopamine transporter, which is a very small protein – too small to be studied in a standard microscope.

“We have used the most powerful microscope in the world to study the structure of the atoms of the dopamine transporter. We have learned how cocaine binds and thus blocks this function, and this is important if we want to be able to stop cocaine from affecting the brain,” says Jeppe Cederholm Nielsen and adds:

“These proteins are so tiny that they are invisible under a microscope – even this extremely powerful microscope. But we used a couple of tricks to work around this and make them visible.”

Want to develop treatment for substance abuse

There is currently no for cocaine abuse, but in the long run the researchers want to that can help people get rid of their addictions.

“Our dream is to find a medical treatment for cocaine abuse. While fewer and fewer people are addicted to drugs in general, an increasing number of people become addicted to cocaine. It is one of the most addictive substances out there, and it is becoming more and more affordable,” says Claus Løland.

The researchers hope their new knowledge of the function of the dopamine transporter will help them understand addiction in general.

“Perhaps we will be able to treat addiction to a number of things, not just cocaine. For instance, addiction to gambling or other substances, which is also caused by a release of dopamine into the brain,” says Claus Løland and adds:

“If we were able to design a drug for addiction in general, we would be able to help a lot of people.”

 

Plants offer fruit to insects to disperse dust-like seeds



Kobe University
Suetsugu Crickets Illustration 

image: 

The shrub-like plant Rhynchotechum discolor produces fruit that are difficult to see from above but suitable for ground-dwelling insects. However, seed dispersal by insects was previously thought to be limited to cases where other dispersers were not available or where fungi-eating plants already had dust-like seeds. The Kobe University botanist SUETSUGU Kenji says, “Our findings challenge the notion that insect-mediated seed dispersal is a special case and suggest that it may be more widespread and ecologically important than previously understood.”

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Credit: ANSAI Shun



Fruit exist to invite animals to disperse the swallowed seeds. A Kobe University research team found that plants targeting insects rather than birds or mammals for this service are more common than previously thought. These plants produce dust-like seeds and fruit suitable for the minute, ground-dwelling animals.

Whether it’s strawberries, apples or grapes, the reason why plants make tasty fruit is to invite and reward animals to also eat and then disperse their seeds, which are smooth and sturdy for just that purpose. Given the size of most seeds, it’s natural that the animals associated with this are mostly birds and mammals. Incidentally, this is also why fruit are often red and dangle from branches. This allows the intended dispersers to easily see and reach the fruit. Insects, on the other hand, while they are often associated with plant pollination, are not seen as important for dispersing ingested plant seeds, mostly because the animals’ minute size wouldn’t allow for the safe passage of the seed through the animal. However, there are plants that produce dust-like seeds embedded in whitish and somewhat translucent fruit that are difficult to see from above and that fall to the ground as soon as they are ripe. And while this makes insects the prime candidates, it has not been known who the actual dispersers are.

The Kobe University botanist SUETSUGU Kenji has an extensive background in studying interactions between plants and arthropods (animals like insects, crabs, and spiders), with a particular focus on dust-seeded plants in Japan. He and his team travelled to Amami-Oshima Island in Japan where a shrub-like plant called Rhynchotechum discolor produces such translucent fruit with dust seeds. The researchers took automated night photographs of the fruit on the ground to find out who eats them and additionally captured insects in the plants’ vicinity (using a mixture of Kirin beer and the soft drink Calpis, both produced in Kobe University’s home region of Kansai). They then put the captured insects into containers to search their excrements for plant seeds and, with a separate feeding experiment, to find out how many of the seeds they eat can germinate.

Their findings, published in the journal Plants, People, Planet, confirmed that insects, specifically camel crickets, were the primary consumers of the fruit. Moreover, the captured insects’ excrement contained numerous seeds, about 80% of which were fully viable. “This result provides the first evidence of insects acting as seed dispersers for a light-harvesting, green plant in regions inhabited by land-dwelling mammals,” says Suetsugu. Previously, seed dispersal by insects was thought to occur only in special situations. There is the case of the weta, gigantic, flightless crickets home to New Zealand, that have taken on ecological functions usually associated with ground-dwelling mammals that don’t exist there. Otherwise, the ability of insects to disperse dust-sized seeds was understood to be largely restricted to heterotrophic plants. These non-green plants don’t harvest light but eat underground fungi and get what nutrients and energy they need from there, so their seeds can be tiny. Suetsugu explains, “Our findings challenge the notion that insect-mediated seed dispersal is a special case and suggest that it may be more widespread and ecologically important than previously understood.”

This discovery excites Suetsugu for another reason: “It also contributes to the understanding of the evolution of dust seeds. Because they are minute and contain minimal energy reserves, their evolution has often been associated with heterotrophy. However, our research on Rhynchotechum discolor suggests that dust seeds can evolve from other selective pressures: Given that smaller seeds are more likely to survive digestive processes, a small seed size could have evolved as an adaptation to convert seed predators into seed dispersers.”

In their paper, the research team notes that the shrub they investigated is likely not unique: “The fruit and seed morphology and coloration of other members of Rhynchotechum parallel those seen in R. discolor, suggesting that seed dispersal by camel crickets is widespread within the genus, which comprises 16 species. In a broader ecological context, similar seed dispersal systems might be found among plants with dust seeds, which are present in at least 13 families.” Suetsugu adds: “We have laid the groundwork for future studies to investigate similar interactions in other regions and with other plant species. This research enhances our knowledge of how plants adapt to their environments and the roles that different organisms play in their life cycles.”

This research was funded by the Japan Science and Technology Agency (grant JPMJPR21D6). It was conducted in collaboration with a researcher from the University of Tokyo.

Kobe University is a national university with roots dating back to the Kobe Commercial School founded in 1902. It is now one of Japan’s leading comprehensive research universities with nearly 16,000 students and nearly 1,700 faculty in 10 faculties and schools and 15 graduate schools. Combining the social and natural sciences to cultivate leaders with an interdisciplinary perspective, Kobe University creates knowledge and fosters innovation to address society’s challenges.

Friday, August 09, 2024

 

Cucumber's genetic core: new insights into centromere structures



Nanjing Agricultural University The Academy of Science
Alignment of CENH3 homologs and localization of CsCENH3 protein and ChIPed DNA on cucumber chromosomes. 

image: 

Alignment of CENH3 homologs and localization of CsCENH3 protein and ChIPed DNA on cucumber chromosomes. a Multiple alignment of CsCENH3 (C. sativus), NtCENH3 (Nicotiana tabacum), AtCENH3 (A. thaliana), OsCENH3 (Oryza sativa) and ZmCENH3 (Zea mays). The protein structure is shown below the sequence. The peptide sequence used to generate anti-CsCENH3 is highlighted in a red box. bg Immunostaining localization of CsCENH3. Interphase nuclei and metaphase chromosomes (blue) were stained with 4′,6-diamidino-2-phenylindole (DAPI). Immunofluorescence signals (c and df and g) are visible within the nucleus (d) and in the centromeric regions of the chromosomes (g). hj FISH signal of DNA precipitated by ChIP using anti-CsCENH3 antibody. Somatic metaphase chromosomes (h and j) that were hybridized to CsCENH3 ChIPed DNA probes. FISH signals (i and j) are visible in the centromeres (j). Scale bars: 10 μm.

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Credit: Horticulture Research



The study provides significant insights into cucumber centromeres by identifying key centromeric satellite sequences and retrotransposons. Researchers found notable differences in centromeric DNA between wild and cultivated cucumbers, highlighting the impact of domestication. Using chromatin immunoprecipitation (ChIP) and sequencing, the study mapped CsCENH3-binding domains and discovered active genes with low transcription in these regions. This comprehensive characterization of cucumber centromeres advances our understanding of genome evolution and provides valuable information for improving genetic maps and breeding programs, potentially enhancing cucumber cultivation and genetic research.

Centromeres are crucial for chromosome segregation during cell division and consist of repetitive DNA sequences. Their structure and evolution vary widely among species, making them a significant research focus. In cucumbers, understanding these variations can provide valuable insights into genome organization and evolution. Due to these complexities and the lack of comprehensive studies in this area, a detailed investigation of cucumber centromeres is necessary to enhance our knowledge of their function and evolutionary dynamics, ultimately contributing to advancements in plant genetics and breeding strategies.

Researchers from the State Key Laboratory of Crop Genetics and Germplasm Enhancement at Nanjing Agricultural University published a study (DOI: 10.1093/hr/uhae127) on May 7, 2024, in Horticulture Research. The study investigates the centromeres of cucumber (Cucumis sativus) using chromatin immunoprecipitation (ChIP) techniques, uncovering centromeric satellite sequences and the prevalence of Ty1/Copia retrotransposons.

The study identified key components of cucumber centromeres, including the centromeric satellite sequence CentCs and Ty1/Copia long terminal repeat retrotransposons. Using ChIP sequencing, researchers mapped CsCENH3-binding domains and uncovered significant differences in centromeric DNA between wild and cultivated cucumbers. They found that the domestication process amplified centromeric DNA, as evidenced by higher CentCs content in cultivated varieties. Additionally, active genes with low transcription levels were identified within CsCENH3 nucleosome regions, marking the first comprehensive characterization of cucumber centromeres. These findings provide new insights into the evolutionary mechanisms of centromeres in the Cucumis genus and enhance our understanding of genome structure. The research also has practical implications, offering valuable information for improving genetic maps and breeding programs, which could lead to the development of superior cucumber varieties with desirable traits.

Dr. Qunfeng Lou, the corresponding author, stated, "This study marks a significant advancement in our understanding of cucumber centromeres. The identification of centromeric sequences and their variations between wild and cultivated cucumbers provides valuable insights into genome evolution and the domestication process. These findings have the potential to inform future research and breeding strategies."

The findings from this study have several important implications. The detailed characterization of cucumber centromeres enhances our understanding of genome structure and evolution, which is critical for genetic mapping and breeding programs. Additionally, the identification of centromeric sequences can aid in the development of new genetic tools and resources, facilitating the improvement of cucumber varieties with desirable traits. This research also contributes to the broader field of plant genomics, providing a framework for studying centromeres in other species.

###

References

DOI

10.1093/hr/uhae127

Original Source URL

https://doi.org/10.1093/hr/uhae127

Funding information

This work was financially supported by National Key R&D Program of China (2021YFD1200200), the Province Key Research and Development Program (BE2021357), the National Natural Science Foundation of China (32272730), and the Fund for Seed Industry Revitalization Project (JBGS (2021)070) and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.

 

Medical issues experienced by women and children after returning from Hamas captivity




Wiley




Among the 250 individuals who were kidnapped during the Hamas terror attack on Israeli towns in October 2023, 19 children and 7 women were released and admitted to Schneider Children's Medical Center of Israel after approximately 50 days in captivity, during a cease-fire deal. A new study published in Acta Paediatrica reports on the physical and psychological state of these returnees upon their return.

The most common clinical findings included significant weight loss, psychological trauma, complications of poor hygiene (such as head lice), and complications of recent shrapnel injuries. Tests revealed that returnees also often had gastrointestinal pathogens and various infectious diseases.

URL upon publication: https://onlinelibrary.wiley.com/doi/10.1111/apa.17355

 

Additional Information
NOTE: 
The information contained in this release is protected by copyright. Please include journal attribution in all coverage. For more information or to obtain a PDF of any study, please contact: Sara Henning-Stout, newsroom@wiley.com.

About the Journal
Acta Paediatrica is a peer-reviewed monthly journal at the forefront of international paediatric research. Published on behalf of the Foundation Acta Paediatrica, it covers both clinical and experimental research in all areas of paediatrics.

About Wiley
Wiley is a knowledge company and a global leader in research, publishing, and knowledge solutions. Dedicated to the creation and application of knowledge, Wiley serves the world’s researchers, learners, innovators, and leaders, helping them achieve their goals and solve the world's most important challenges. For more than two centuries, Wiley has been delivering on its timeless mission to unlock human potential. Visit us at Wiley.com. Follow us on FacebookXLinkedIn and Instagram.

 JUST ASKING

Do dieticians have weight biases towards themselves and others?




Wiley





In a survey-based study, UK dietitians exhibited significant weight stigma, both towards themselves and towards others.

The study in the Journal of Human Nutrition and Dietetics involved an online survey completed in 2022 by 402 registered dietitians aged 20–70 years old. Most respondents reported personally experiencing weight stigma prior to (51%) and after becoming (59.7%) registered dieticians, and nearly a quarter (21.1%) felt that their weight influenced their own ability to perform as a dietitian.

Weight stigma was experienced across the weight spectrum. Participants reported explicit (or conscious) weight bias attitudes, moderate beliefs that obesity is controllable, and implicit (or unconscious) anti-fat bias.

“The study highlights the need to address weight stigma and its implications within the dietetic profession,” the authors wrote.

URL upon publication: https://onlinelibrary.wiley.com/doi/10.1111/jhn.13337

 

Additional Information
NOTE: 
The information contained in this release is protected by copyright. Please include journal attribution in all coverage. For more information or to obtain a PDF of any study, please contact: Sara Henning-Stout, newsroom@wiley.com.

About the Journal
The Journal of Human Nutrition and Dietetics publishes articles related to nutritional science, clinical nutrition, dietetics practice, and public health nutrition. The scope of the journal recognises the breadth and multidisciplinary nature of nutrition and dietetics research and will consider material from all facets of the discipline.

About Wiley
Wiley is a knowledge company and a global leader in research, publishing, and knowledge solutions. Dedicated to the creation and application of knowledge, Wiley serves the world’s researchers, learners, innovators, and leaders, helping them achieve their goals and solve the world's most important challenges. For more than two centuries, Wiley has been delivering on its timeless mission to unlock human potential. Visit us at Wiley.com. Follow us on FacebookXLinkedIn and Instagram.

 

Can nanomaterials enhance plant tolerance to high soil salt levels?



Wiley





Soil salt concentrations above the optimal threshold for plant growth can threaten global food security by compromising agricultural productivity and crop quality. An analysis published in Physiologia Plantarum examined the potential of nanomaterials—which have emerged over the past decade as a promising tool to mitigate such “salinity stress”—to address this challenge.

Nanomaterials, which are tiny natural or synthetic materials, can modulate a plant’s response to salinity stress through various mechanisms, for example by affecting the expression of genes related to salt tolerance or by enhancing physiological processes such as antioxidant activities.

When investigators assessed 495 experiments from 70 publications related to how different nanomaterials interact with plants under salinity stress, they found that nanomaterials enhance plant performance and mitigate salinity stress when applied at lower dosages. At higher doses, however, nanomaterials are toxic to plants and may even worsen salinity stress.

Also, plant responses to nanomaterials vary across plant species, plant families, and nanomaterial types.

“Our analysis revealed that plants respond more positively to nanomaterials under salt stress compared with non-stressed conditions, indicating the ameliorative role of nanomaterials,” said corresponding author Damiano R. Kwaslema, MSc, of Sokoine University of Agriculture, in Tanzania. “These findings pave the way for considering nanomaterials as a future option for managing salinity stress.”

URL upon publication: https://onlinelibrary.wiley.com/doi/10.1111/ppl.14445

 

Additional Information
NOTE: 
The information contained in this release is protected by copyright. Please include journal attribution in all coverage. For more information or to obtain a PDF of any study, please contact: Sara Henning-Stout, newsroom@wiley.com.

About the Journal
Physiologia Plantarum advances the understanding of primary mechanisms of plant development, growth, and productivity, as well as plant interactions with the biotic and abiotic environment.

About Wiley
Wiley is a knowledge company and a global leader in research, publishing, and knowledge solutions. Dedicated to the creation and application of knowledge, Wiley serves the world’s researchers, learners, innovators, and leaders, helping them achieve their goals and solve the world's most important challenges. For more than two centuries, Wiley has been delivering on its timeless mission to unlock human potential. Visit us at Wiley.com. Follow us on FacebookXLinkedIn and Instagram.