Wednesday, August 26, 2026

 

Biocultural Minga: Chile and Colombia forge a new path for south-south cooperation on biodiversity



How can science and communities work together to tackle the biodiversity crisis? Chile and Colombia are forging a new model of south-south cooperation to drive socioecological solutions with global impact.




Instituto de Ecologia y Biodiversidad

Biocultural Minga Participants in Chiloé 

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Participants from the Institute of Ecology and Biodiversity (IEB) and the Humboldt Institute during the Biocultural Minga at the Senda Darwin Biological Station in Chiloé, Chile.

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Credit: Xuksa Kramcsak / Institute of Ecology and Biodiversity (IEB)





These were the questions that brought together Chile’s Institute of Ecology and Biodiversity (IEB) and Colombia’s Humboldt Institute, two of Latin America’s leading scientific institutions dedicated to biodiversity and sustainability research. At a time when the international community is seeking new responses to the climate and ecological crises, the two institutions are advancing an alliance that positions South-South cooperation as a strategic platform for co-producing knowledge, strengthening capacities, and developing innovative, place-based solutions.

Building on their experience working with communities, indigenous peoples, local governments, and civil society organizations, this collaboration seeks to build a Latin American model of socioecological research that integrates science, local knowledge, and collective action to address the environmental challenges of the 21st century.

During the last week of July, 17 participants—including members of the IEB associated with its Long-Term Socioecological Research Integration Program, representatives of Colombia’s Humboldt Institute, particularly its Center for Social Appropriation and Center for Socioecological Studies and Global Change, as well as local territorial stakeholders—took part in a series of working sessions at the Senda Darwin Biological Station in Chiloé, southern Chile.

The meeting aimed to advance the design of a collaborative model for socioecological research between Chile and Colombia, strengthening regional scientific cooperation and facilitating its implementation across different Latin American territories through knowledge co-production, the exchange of experiences, and joint work with local stakeholders.

Over three days, researchers, communities, and territorial stakeholders engaged in dialogue and reflection using an exchange matrix designed to guide discussions between the two institutes. The matrix posed strategic questions about the knowledge and methodologies to be shared, forms of collaboration, expected outcomes, and mutual learning.

The process placed particular emphasis on socioecological restoration, inter- and transdisciplinary research, the integration of local and Indigenous knowledge, territorial governance, knowledge co-production, biocultural heritage, and the development of a south-south cooperation agenda aimed at generating shared solutions to the socioenvironmental challenges currently facing Chile and Colombia.

Through presentations, discussion sessions, a visit to the territorial experience of the Catruman community and its sustainable watershed management model, and participatory workshops, participants identified shared principles, methodological approaches, and challenges for strengthening collaborative socioecological research. This work helped move the partnership from an exchange of experiences toward defining guidelines and agreements for launching pilot joint-research initiatives and consolidating a collaborative model between the two countries.

The meeting was held within the framework of the Collaboration Agreement signed by the IEB and the Humboldt Institute in October 2025, whose purpose is to strengthen biodiversity research, conservation, and knowledge management in Latin America.

Commitments Moving Forward

As a result of the meeting, the participating institutions and territorial organizations agreed to consolidate an alliance based on the principles of biocultural diversity, reciprocity with nature, and recognition of territories as living spaces for learning and collaboration.

Within this framework, they committed to strengthening and systematizing experiences of joint work among science, communities, and local governments; creating the Biocultural Minga community of practice to promote the exchange of methodologies, capacity building, and the development of collaborative projects; developing a pathway for policy impact to help incorporate these experiences into planning instruments and public policies; promoting, over the longer term, a Latin American exchange school focused on biocultural diversity and socioecological restoration; and developing a Chiloé Manifesto as an expression of their ethical and strategic commitment to collaborative research and to strengthening relationships between people and nature.

 

IEB Positions Social Innovation as a Key Pillar of Knowledge Transfer

The working sessions were organized by the IEB’s integrative program, Long-Term Socioecological Research. In this context, IEB manager Fernando Valenzuela highlighted the value of bringing together researchers, professionals from the Humboldt Institute, and representatives of the territories where the IEB works to strengthen a shared vision of socioecological research.

“All the perspectives brought together here have helped us understand the importance of consolidating the work that has been carried out at the biological stations through the Long-Term Socioecological Research Integration Program. Both stations host highly relevant research activities and processes, with more than 20 and even 30 years of history, generating information and data on different ecosystems and their dynamics. However, we need to make much greater progress in incorporating the social dimension and in understanding how people who interact with these territories can, on the one hand, take ownership of scientific knowledge and, at the same time, convey and share local and traditional knowledge with the scientists carrying out this monitoring,” he said.

Paula Gatica, coordinator of the Long-Term Socioecological Research Integrative Program and organizer of the event, said the meeting demonstrated the value of collaboration among institutions.

“Sharing experiences, lessons learned, and challenges allows us to adapt and strengthen good practices, enhancing the socioecological approach of long-term research. In turn, this knowledge can be transferred and implemented across the different sites that make up the Chilean Long-Term Socio-Ecological Research Network (LTSER-Chile), strengthening the network and promoting research that is increasingly connected to the needs of local territories.”

Gatica added that one of the main lessons drawn from their Colombian colleagues is their experience in the social appropriation of knowledge and the development of participatory processes with territorial stakeholders.

The mapping exercises carried out in Fray Jorge and Senda Darwin allowed us to identify these key stakeholders and understand their interests and needs. The Colombian experience can help us move from that diagnosis toward concrete actions and more lasting collaborations, strengthening territorial governance and integrating scientific knowledge with local knowledge.”

Building on this point, Valenzuela said one of the IEB’s goals is to consolidate socioecological efforts and approaches while incorporating these criteria into the way science itself is conducted from a socioecological perspective.

“This will allow us to improve the impact that science can have, not only on public policy, but also on productive sectors and in the territories where decisions are made.”

Along the same lines, Marcela Verdugo, director of the IEB’s Development and Technology and Knowledge Transfer Unit (UDTT), emphasized that the meeting encourages progress toward more collaborative models in which science does not simply reach territories but enters into dialogue with the knowledge, experiences, and capacities of the people who live there.

Knowledge co-production creates opportunities to foster social innovation processes, generate solutions that are relevant to each context, and strengthen capacities that remain within the territories. The challenge now is to sustain these alliances and ensure that the lessons built together translate into concrete actions and impacts,” she said.

 

Reflecting Together to Expand Socioecological Impact

From the Humboldt Institute, Marcela Lozano, manager of the Center for Social Appropriation, highlighted the collaborative process and collective reflection.

“For us, it has been very inspiring to exchange the experiences we have had as institutes, because we often have too few opportunities to reflect on these dynamics, which allow us to strengthen our processes—especially those seeking to connect social processes with the scientific and biological research carried out by our institutions.”

Regarding the challenges of inter- and transdisciplinary research, which involves the active participation of communities and dialogue among different knowledge systems, she said it is important to “recognize other ways of seeing the world and understanding biodiversity, and to understand that local knowledge also enriches the scientific knowledge produced in the territories.”

Lozano noted that “we act as a mirror,” reflecting the realities of both countries, and that these situations must therefore be addressed ethically and responsibly while promoting equity. This space, she explained, not only allows participants to share the tensions that arise among different disciplines, but also to jointly build new ideas, proposals, and aspirations.

It has been extremely valuable to discover that, despite our different contexts, we share concerns and common commitments regarding governance, socioenvironmental conflicts, and participatory socioecological restoration. Although our institutional trajectories are different, we are driven by people’s well-being and by the goal of building pathways toward sustainability,” she said.

Meanwhile, Omar Ruiz Nieto, a researcher at the Humboldt Institute and leader of the Governance and Conflict Transformation research line at the Center for Social Appropriation, highlighted the opportunity to learn about the water-management experience of the Catruman community and its example of community-led governance supported by the IEB, which provided participants with key elements for interaction, knowledge sharing, and exchange.

“I would highlight the constructive dialogue we have been developing to think about and conceptualize socioecological restoration—in other words, understanding how social dimensions are incorporated into restoration processes. This integrated perspective, bringing together the social and ecological dimensions, allows us to open up new avenues for analysis and action, including work alongside communities,” he said.

Ruiz Nieto also noted that each research process carried out at the IEB’s long-term study sites—Fray Jorge and Senda Darwin—provides an opportunity for the Humboldt Institute to rethink its own biodiversity monitoring processes and consider how it might move toward a long-term monitoring framework with a significant social component.

“I believe there are important opportunities for learning and joint development that we could pursue,” he said.

Mónica Ortiz, a recently appointed IEB researcher and academic at the University of Concepción, who facilitated the dialogue and working matrix between the two institutes, highlighted the action-research process and the synergy between the two countries.

“We had the opportunity to exchange experiences based on the recognition that ecosystems are deeply coupled with social systems, that communities are part of nature, and that this relationship must also be reflected in governance, in the management of socioenvironmental conflicts, and in the way we conduct research.”

From her perspective, Ortiz highlighted the inspiration provided by Chiloé and the concept of the minga, in which everyone contributes to building something together.

“We have the opportunity to share, co-create, and build a common path. This is extremely important in the context of regional cooperation and from the perspective that we are part of the Global South, at the frontiers of science,” she emphasized.

She also underscored the value of short-, medium-, and long-term collaboration deeply rooted in communities.

The challenge now is to consider how this collaborative model can also become a model for socioecological restoration, engagement with territories, and, eventually, influence on public policy at both the local and international levels.

“I am very excited about everything we have envisioned as a result of this meeting: new meetings, joint activities, the possibility of publishing a book, and participating in international biodiversity forums, while also recognizing the Humboldt Institute’s leadership in these processes,” Ortiz said.

 

Committed Voices and Leaders from Chiloé and Monkul

More than an academic exchange, this meeting demonstrates that new forms of south-south cooperation are emerging in Latin America to jointly address the global socioenvironmental crisis. In a context marked by profound fragmentation—between nature and culture, the natural and social sciences, and different knowledge systems—the experience in Chiloé shows that it is possible to build bridges between scientific research, local knowledge, and place-based action.

These alliances not only strengthen knowledge generation but also create opportunities to advance socioecological transitions aimed at peace, sustainability, and biodiversity conservation.

Among the places where the IEB has collaborated and established ties with territorial communities to advance biocultural conservation are the municipality of Ancud, near the Senda Darwin Biological Station, and Monkul, on the coast of Chile’s Araucanía Region. At both sites, work has focused on community well-being through a socioecological approach.

Juan Corrales, from the Municipality of Ancud, reflected on the importance of including local stakeholders in the design of research policies and in defining the objectives of research centers over the short, medium, and long term, noting that this gives scientific work greater meaning.

Today we are living through a critical moment in human history, when it is essential to ask ourselves what we conduct research for, why we do it, where we want to go, and what gives meaning to our actions. And those answers can only be built by bringing together academia, institutions, and the local stakeholders who experience this reality every day—a reality that is dynamic, changing, and under constant tension,” he said.

Corrales also noted the importance of seeing how researchers have been able to recognize the value of their own work and of the fraternal experience of coming together, learning, and creating.

“There is a sense of self-worth that belongs not only to the territories, but also to those who produce knowledge, and that is something we must value. It is important to recognize the people at the heart of these stories, with their strengths, concerns, and challenges. Sharing these experiences, communicating them, and supporting them is essential, because addressing the multiple crises we face requires a joint and coordinated effort.”

Meanwhile, Estela Nahuelpán, a leader of the Mateo Nahuelpán Lafkenche community on the coast of the Carahue and a representative of Ekos de Monkul, also took part in the meeting and learned about the approaches being developed in both countries around socioenvironmental restoration. “I believe both teams have been building significant strengths, creating enormous potential for future joint work. I think it is extremely important to highlight the participation of local communities, different stakeholders and, above all, Indigenous Peoples in the territories of both countries. Their perspectives and ways of understanding the world can help enrich research projects, while these projects can, in turn, make a highly valuable contribution to the territories and their people,” she said.

Finally, this institutional collaboration takes on strategic importance given that the Humboldt Institute was designated as a Subregional Technical and Scientific Cooperation Support Centre for Latin America and the Caribbean under the Convention on Biological Diversity (CBD) to support implementation of the Kunming-Montreal Global Biodiversity Framework (KMGBF).

Together with the IEB—which receives funding from Chile’s National Agency for Research and Development (ANID)—the two institutions have consolidated a cooperation agenda aimed at promoting the generation and application of scientific knowledge, exchanging experiences, and strengthening capacities to respond to biodiversity conservation challenges across the region.

With extensive scientific experience and active participation in international advisory processes, both institutes contribute to assessments by the Intergovernmental Panel on Climate Change (IPCC) and the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES), as well as to the Conferences of the Parties (COPs) of major environmental conventions.

This alliance demonstrates how scientific cooperation among countries of the Global South can become a driver of innovation, policy impact, and collective action, projecting collaborative solutions from Latin America to address the global challenges of biodiversity loss and climate change.

 

Early nicotine vapor exposure increases nicotine seeking later in life, UTEP study finds





University of Texas at El Paso

Early Nicotine Vapor Exposure_01 

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Ian Mendez, Ph.D. (left), associate professor of pharmacy and chair of the Department of Pharmaceutical Sciences at The University of Texas at El Paso, and Liliana Maynez-Anchondo (right), a doctoral student in the UTEP Department of Psychology, are two of the coauthors of a study that found nicotine vaping during adolescence may leave a lasting imprint on the brain’s reward system, driving nicotine-seeking behavior well into adulthood.

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Credit: The University of Texas at El Paso.






EL PASO, Texas (Aug. 26, 2026) – Nicotine vaping during adolescence may leave a lasting imprint on the brain's reward system, driving nicotine-seeking behavior well into adulthood even after long stretches without any exposure, according to new research led by scientists at The University of Texas at El Paso.

The findings, published in the journal Psychopharmacology, come from a study by a UTEP team working across the School of Pharmacy and the Department of Psychology in the College of Liberal Arts.

Using an animal model of e-cigarette use, the researchers tracked reward-seeking behavior from adolescence through late adulthood.

The most striking result came in the study's final phase, the team said, which was conducted in late adulthood. Following a lengthy period of abstinence, subjects exposed to nicotine vapor early in life sought out nicotine significantly more often than those never exposed. The behavior persisted despite the long gap, pointing to durable changes in how the brain responds to nicotine.

"What we found is that the effects of early nicotine vapor exposure don't simply fade with time," said Ian Mendez, Ph.D., associate professor of pharmacy at UTEP, chair of the pharmaceutical sciences department and the study's corresponding author. "Even after an extended period with no nicotine whatsoever, that early exposure was still shaping behavior later in life. That matters enormously for the millions of young people who began vaping as teenagers and are now carrying the habit into adulthood."

A second experiment examined whether nicotine vapor itself would motivate sustained, effortful work. When each delivery required escalating effort, nicotine drove only modest responding. When the effort required was low, subjects clearly favored a port delivering nicotine. The pattern is consistent with earlier findings that nicotine is a comparatively subtle reinforcer, and it illustrates how challenging vaping behavior can be to capture in a pre-clinical model.

The work lands at a moment of sustained concern about youth vaping. E-cigarettes remain the most commonly used nicotine product among American adolescents, according to data from the FDA National Youth Tobacco Survey (NYTS). Nicotine use is the leading preventable cause of disease, death and disability in the United States, yet the cumulative health effects of vaping that begin in the teenage years and continue into adulthood remain poorly understood, the researchers noted.

"Our faculty and students are confronting a public health crisis that reaches into many households,” said José O. Rivera, Pharm.D., founding dean and professor at UTEP's School of Pharmacy. “And they are doing it with the rigor and creativity that define this University.”

The authors note that the study examined only male subjects and call for future work that includes female subjects, citing well-documented sex differences in the mechanisms driving nicotine use disorder. They also recommend that subsequent studies test how the unique additives and metabolites in e-liquids – the materials that are heated and converted into inhalable aerosol by electronic cigarettes and vaping devices – affect the brain and behavior.

The study was supported by a grant from the National Institutes of Health.

About The University of Texas at El Paso

The University of Texas at El Paso is America's leading Hispanic-serving university. Located at the westernmost tip of Texas, where three states and two countries converge along the Rio Grande, 84% of our 26,000 students are Hispanic, and more than half are the first in their families to go to college. With respect to research, UTEP is in the top 5% of universities in America and offers 169 bachelor's, master's and doctoral degree programs at the only open-access, top-tier research university in America.

Meet the future of fashion: Clothes made alive by fungi




Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences






Fungal mycelium is a promising renewable material, but conventional processing generally eliminates the living activity that could support growth, repair and environmental response.

Recently, a study published in Science Advances and led by Zhong Chao from the Shenzhen Institutes of Advanced Technology (SIAT) of the Chinese Academy of Sciences developed a flexible living textile based on caterpillar fungus, or Cordyceps militaris.

The researchers grew caterpillar fungus in liquid culture, where it formed spherical mycelial pellets composed of intertwined fungal filaments. After being collected and placed into molds, the pellets naturally bonded during mild drying to form free-standing sheets without additional scaffolds or adhesives.

Glycerol treatment reduced the brittleness of the dried sheets, allowing them to be folded, cut and sewn. A rope twisted from four narrow strips could support a one-kilogram weight.

The team also introduced additional biological functions. Engineered yeast cells generated pale blue, red, orange and purple colors, while nutrient-induced aerial hyphae enabled patterned growth and self-cleaning surfaces.

Melanin-rich aerial hyphae from Aspergillus niger further provided ultraviolet protection. Under humid, nutrient-rich conditions, some cells could become active again, enabling regrowth and repair; in soil composting tests, the material almost completely degraded in just over 40 days.

The researchers assembled the functionalized sheets into a prototype dress, demonstrating that the material could be shaped and sewn into larger structures. Further work is needed to improve manufacturing consistency, washability, abrasion resistance and control over biological activation.

As the authors noted, "Beyond textile analogs, this paradigm provides a foundation for the broader development of adaptive, living material platforms across biomedical, environmental, and architectural domains."

 

Heavy social media use tied to depression



Depression linked to 2.5 hours or more of daily scrolling




University of Cincinnati

SOCIAL MEDIA 

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UC Professor Hans Breiter and his research partners used a machine learning model to examine links between social media use and self-reported depression. 

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Credit: Andrew Higley





Social media use is a better predictor of depression than many other common factors such as one’s job and educational background or time spent on other activities such as playing video games, surfing the web or watching TV.

Meanwhile, researchers observed a correlation with self-reported depression in survey respondents starting at 2.5 hours of daily scrolling.

Researchers from the University of Cincinnati and Northwestern University assessed 11 years of data from the annual Media Behaviors and Influence Study starting in 2014 and found that social media use was among the most important predictors of self-reported depression.

“When it comes to depression and social media, the argument is salient across scientific test after test of those 11 years,” corresponding author and UC Professor Hans Breiter said.

“It’s a damning picture. Social media is harmful for individuals across the lifespan. We should not subject ourselves to a product using addiction science to optimize our viewing time.“

The study was published in the Nature Portfolio journal Mental Health Research.

UC researchers collaborated with co-lead author and Professor Emeritus Martin Block at Northwestern University. While numerous studies have documented associations between social media use and negative outcomes such as mental health conditions, especially depression, none have used AI to show these relationships persist in adults.

Researchers examined 183,300 anonymized surveys from adults ages 18 to 70 from the Media Behaviors and Influence Study conducted annually since 2002 by Prosper Insights & Analytics. The survey asks 1,318 questions across topics and the respondent’s health across 31 measures, including self-reported depression.

They used a machine learning model that was evaluated with tools designed to ensure its accuracy and calibration. Using this model, they found an increasing relationship between social media use and depression.

“Much of the published research has focused on younger populations, single datasets or broad measures of screen time. We need studies that distinguish social media from other digital activities and examine whether the same associations appear across different populations and years,” co-lead author and UC Research Associate Vikram Suresh said.

Researchers observed a correlation with self-reported depression among respondents who spent at least 150 minutes on social media per day. They observed the same result in each of the 11 years studied.

“Mental health and happiness are about engaging the world and having purpose, not passively watching something for hours,” Breiter said.

UC doctoral student and study co-author Johnathan Avant said people long have suspected that excessive social media use could have negative health effects.

“You can see trends on social media where they talk about the mental health toll it has on them,” Avant said.

Researchers found that social media increasingly became a bigger predictor of depression during the 11 years studied, eclipsing many other factors like TV viewing or web surfing.

UC senior research associate and co-author Nicole Vike said consumption of social media has changed over the years.

“Initially, social media was a social outlet with people you knew,” Vike said.

Now, people typically consume content made by strangers, she said.

“It’s crazy how addictive it can be. Look at chronically online content creators,” Vike said. “They often have to take a monthslong hiatus from being online for their mental health.”

The study was supported with grants from the Office of Naval Research and a donation from UC alumnus Jim Goetz.

While the $300 billion industry has become ubiquitous in daily life, UC’s senior research associate and co-author Sumra Bari said there are ways people can limit time spent on social media like setting timers, turning off notifications and fully logging out of accounts after every session to make it harder to scroll again out of habit.

But it might be unrealistic for people to simply turn it off, she said.

“There is a lot of social pressure to stay connected and be out there. So being aware of the risks as early as possible is important,” she said.

Professor emeritus Block noted, “Social media is a common marker of our cultural age much like the automobile was more than a century ago. But social media has an important negative effect through its association with depression, much like the automobile is associated with injuries from crashes and pollution.”

Breiter said their findings suggest it’s in society’s best interest to find ways to mitigate the harms of today’s deeply online culture.

“This is a wake-up call,” he said.

 

New approach cuts sepsis diagnosis from days to hours, researchers report




Penn State
Blood Culture Vials Photo 

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The figure showcases the “broth” used to monitor red blood cell depletion in whole blood spiked with one colony-forming-unit of E. coli bacteria, each incubated at different orbital shaking speeds — left to right: 0 RPM, 65 RPM, 120 RPM and 200 RPM — after four hours of incubation. This culturing raises a bacteria-rich, plasma-like layer of bacteria to the top of the vials, while clusters of stuck blood cells known as a Rouleaux formation sink to the bottom.

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Credit: Provided by Pak Kin Wong






UNIVERSITY PARK, Pa. — Sepsis, a life-threatening condition that stems from the body trying to fight off an infection, plagues over 1.5 million patients a year in the U.S. alone, with one in three deaths recorded in the hospital attributed in part to sepsis. Despite the severity of the condition, which can kill in as little as 12 hours, it takes between two and seven days for most traditional approaches to definitively identify sepsis-causing bacteria in blood infections.  

A team led by an engineer at Penn State has developed a way to condense the days-long diagnosis timeline to just hours. The new approach rapidly grows the bacteria present in collected blood samples, intermittently analyzing the samples with advanced techniques that help scientists identify the specific pathogens causing infection. The researchers reported in a paper published today (Aug. 26) in Science Advances that their approach facilitated faster diagnosis and could help clinical decision-making that avoids worsening antibiotic resistance in the bacterial strains causing infections.  

“For a physician, ‘what actually caused this infection and how should it be treated?’ are the most important questions when it comes to the timely management of a bloodstream infection,” said corresponding author Pak Kin Wong, professor of biomedical engineering and of mechanical engineering. “We are developing a comprehensive diagnostic platform that rapidly tells physicians both the specific bacteria causing a bloodstream infection, as well as the ideal antibiotic to treat the infection, before sepsis ever sets in.”  

With a bloodstream infection, it is critical to find and neutralize the cause as quickly as possible, before it triggers a septic response from the body, Wong explained. Physicians must not only detect the presence of bacteria — they must also identify the specific pathogens, as well as the best antibiotic for treatment. The dire stakes of a false positive or negative complicate this further, as every hour counts when treating a bloodstream infection, Wong said.  

“This is not like a COVID test, where we are checking to see a specific virus is present in a patient’s system,” Wong explained. “Many different bacteria can cause sepsis, and they may respond differently to treatment. Therefore, analysis must be thorough to ensure the best treatment is prescribed.”  

Bloodstream infections are responsible for about 40% of all sepsis cases that lead to hospitalization. The complex biological makeup of the blood and the low pathogen loads needed to trigger sepsis make pinning the cause of a bloodstream infection time-consuming. To identify the bacteria causing a bloodstream infection, current best practices require bacterial culturing: Blood samples are enriched over a few days so that present bacteria grow to measurable levels. Then, technicians further analyze the samples to identify the specific bacteria, a process that adds another day or two to diagnosis.  

Streamlining bloodstream infection diagnosis is not a novel idea, with several commercial products offering culture-free blood testing already on the market. However, to reduce diagnostic time, these products provide less comprehensive and less sensitive readings, Wong said.  

To accelerate diagnosis without sacrificing accuracy, the team had to rethink culturing. Traditionally, bacterial growth in a cultured blood sample is measured through the carbon dioxide released by the bacteria. When this change in carbon dioxide levels confirms the presence of pathogens, bacteria are separated from the blood sample and analyzed. The team’s new approach, called STREAM, fast-tracks this culturing by facilitating rapid bacterial growth, while isolating and analyzing the pathogens inside simultaneously — blood samples are mixed in a specialized “broth” that separates whole blood cells from the individual bacteria found in the sample during culturing. 

Molecular analysis, a process known formally as barcoding, allows the team to detect tiny fragments of genetic information from isolated bacteria. From these smaller samples collected intermittently during culturing, the researchers can name the specific bacterial species present.  

“Instead of sampling at a particular endpoint after culture, we collect samples at multiple time points throughout the culture process,” Wong said. “This approach maintains robust bacterial detection while minimizing the time to results.”  

These smaller samples are then subjected to a series of new, single-cell-based techniques that allow researchers to analyze a bacterium with microscopic imaging. These images are then analyzed by computer algorithms the team developed to eliminate visual clutter from the images, helping physicians determine the specific bacteria causing infection. These analyses also suggest which antibiotics the strain is susceptible to and any existing antibiotic resistance the strain may have.  

The team tested their approach with about 100 positive bloodstream infection samples donated by patients and stored at Penn State Hershey Medical Center’s clinical microbiology laboratory. The researchers found that combining these techniques offered comprehensive diagnosis in as little as seven hours, enabling confident identification of the infection causing pathogen from whole blood.  

“Using single-cell analysis technologies developed by our team, we can accurately identify pathogens in blood even when they are present at very low concentrations,” Wong said. “Identifying the pathogen alone is not enough; we must also determine which antibiotics are effective against it. This led us to integrate antibiotic susceptibility testing into the same process, providing physicians with the information needed to select the most appropriate treatment.”  

Wong said it is important to note that just over 4% of these samples were classified as "very major errors," meaning a bacterium was inaccurately identified as resistant or susceptible to an antibiotic, which could cause the prescribed treatment to be ineffective. The team acknowledged that although some large hurdles need to be addressed before clinical implementation — including this relatively high major error rate and difficulties that could arise when testing blood from patients who had already received antibiotics — the framework offers a promising foundation for detecting and treating a leading cause of death around the world faster than ever before.  

“We are integrating artificial intelligence and lab automation to make the entire process even more efficient and accurate,” Wong said. “This framework is scalable, so the list of pathogens we can detect could feasibly be expanded. We believe we could adapt this approach to identify infections originating from sources other than bacteria, like fungal infections. We hope to work closely with physicians at Penn State College of Medicine to move towards more clinical studies and, eventually, clinical adoption.”  

Other co-authors affiliated with Penn State include April M. Bobenchik, associate professor of pathology and laboratory medicine; Siew Mei Chin, a biomedical engineering doctoral candidate at the time of work who has since graduated and is now a postdoctoral researcher at Stanford University’s School of Medicine; Evgenii Kovtunov, a postdoctoral researcher at the time of the work who is now a medical and public health microbiology fellow at the University of Rochester; and Emma Epiphaniou, an undergraduate researcher at the time of the work who earned her bachelor’s degree in biomedical engineering from Penn State.  

Additional co-authors include Samuel Yang, professor of emergency medicine; Joseph C. Liao, professor of urology; and Kathleen E. Mach, a senior research scientist, all with Stanford University’s School of Medicine.  

This work was supported by the One Health Microbiome Center Interdisciplinary Innovation Fellowship and the Leighton Riess Graduate Fellowship, as well as the National Institutes of Health’s National Institute of Allergy and Infectious Diseases under award number R01AI153133. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funders.  

At Penn State, researchers are solving real problems that impact the health, safety and quality of life of people across the commonwealth, the nation and around the world.  

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