Fruit juice offers a fresh take on kombucha

American Chemical Society

 

image: 

Researchers brewed kombucha (left) and kombucha-like beverages from passion fruit (center) and apple (right) juices and found that the apple-based drink had the most flavonoids and ranked most favorable among a panel of taste testers.

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Credit: Adapted from ACS Agricultural Science & Technology 2024, DOI:10.1021/acsagscitech.4c00372

Kombucha is a fizzy, tangy drink made by fermenting tea. But brewers are now fermenting other plant-based drinks to explore nutritional properties and flavors. Researchers in ACS Agricultural Science & Technology compared the biochemistry and flavor of kombucha with brews made from apple and passion fruit juices. They found that the apple beverage contained high levels of bioactive compounds called flavonoids and ranked highly among taste testers, signaling its promise as a kombucha alternative.

To make kombucha, brewers ferment sweetened tea with a spongy disk of microbes known as a SCOBY, or symbiotic culture of bacteria and yeast. The resulting beverage contains beneficial bacteria from the fermentation process and bioactive compounds from the tea, including flavonoids, phenolics and anthocyanins that may have antioxidant and anti-inflammatory properties. Few studies have investigated whether liquids other than tea could be brewed as kombucha-like beverages with boosted antioxidant levels or unique flavors. So, Socorro Vanesca, Frota Gaban and coworkers fermented antioxidant-rich apple and passion fruit juices with a SCOBY to find out.

After fermenting apple juice, passion fruit juice, and tea in separate jars for 10 days at room temperature, the researchers measured the levels of several bioactive compounds in each brew and found that:

• The apple beverage had the highest level of flavonoids, followed by kombucha and the passion fruit drink.
• The kombucha and apple beverages had comparable levels of phenolic compounds that were higher than those of the passion fruit beverage.
• All three brews had similar amounts of anthocyanin, a red-colored antioxidant.

The researchers asked 12 volunteer taste testers to evaluate each beverage’s color, aroma and flavor. The taste testers noted an amber color for the apple beverage, yellow for passion fruit and gold for the kombucha. The volunteers reported stronger, fruitier aromas for the fermented fruit juices compared with the tea. However, the passion fruit drink tasted more bitter than the sweeter apple and tea drinks, both of which received equal votes as the favorite beverage. Because fermented apple juice has more flavonoids and a pleasant taste compared with the other beverages, the researchers say it could be a successful alternative to kombucha made from tea. They plan to explore health benefits and flavors of other fermented fruits in the future.

The authors acknowledge funding from the Coordination for the Improvement of Higher Education Personnel.

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The American Chemical Society (ACS) is a nonprofit organization chartered by the U.S. Congress. ACS’ mission is to advance the broader chemistry enterprise and its practitioners for the benefit of Earth and all its people. The Society is a global leader in promoting excellence in science education and providing access to chemistry-related information and research through its multiple research solutions, peer-reviewed journals, scientific conferences, e-books and weekly news periodical Chemical & Engineering News. ACS journals are among the most cited, most trusted and most read within the scientific literature; however, ACS itself does not conduct chemical research. As a leader in scientific information solutions, its CAS division partners with global innovators to accelerate breakthroughs by curating, connecting and analyzing the world’s scientific knowledge. ACS’ main offices are in Washington, D.C., and Columbus, Ohio.

Registered journalists can subscribe to the ACS journalist news portal on EurekAlert! to access embargoed and public science press releases. For media inquiries, contact newsroom@acs.org.

Note: ACS does not conduct research but publishes and publicizes peer-reviewed scientific studies.

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Journal

ACS Agricultural Science & Technology

DOI

10.1021/acsagscitech.4c00372 

Article Title

“Physicochemical Properties, Antioxidant Activity, and Sensory Profiles of Kombucha and Kombucha-Like Beverages Prepared Using Passion Fruit (Passiflora edulis) and Apple (Malus pumila)”

Increased antioxidants and phenolic compounds produced in salted red perilla leaves during Japanese apricot pickling

Antioxidant content and activity are increased during the processing and digestion of Japanese apricots pickled with salted red perilla leaves

Chiba University

 

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Sampling was conducted at three stages: during simple salt pickling, after 30 days of pickling with salted perilla leaves, and after 60 days of pickling with salted perilla leaves. The resulting Shiso-zuke Umeboshi had a distinctive red appearance.

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Credit: Prof. Yukiharu Ogawa from Chiba University

The diverse biochemical composition of Japanese apricot fruits explains their broad spectrum of action on the human body. The high levels of key phenolic compounds and hydroxycinnamic acids contribute to various health benefits including anti-inflammatory, antioxidant, and antimicrobial properties.

A recent study published in Food Research International on July 19, 2024, led by Prof. Yukiharu Ogawa and Jutalak Suwannachot from Chiba University, quantitatively evaluated the changes in phenolic compounds and antioxidant activity during the production of Shiso-zuke Umeboshi (PP). The study also simulated the digestive process to characterize the release of these compounds and their antioxidant activity.

“Some of the benefits have been observed in pickling with red perilla (PP), but the current data are still limited. Many recent efforts have been focused on creating functional foods, which are designed to provide health benefits and essential nutrients in addition to the traditional micronutrients. However, there remains uncertainty regarding the specific effects of apricot pickled with red perilla (Shiso-zuke Umeboshi, PP). This motivated our study,” explains Prof. Ogawa.

In this study, the production of perilla-pickled apricots involved three methods: simple salt pickling (SP), pickling with salted perilla leaves for 30 days (30-PP), and pickling with salted perilla leaves for 60 days (PP). The pickle samples underwent ultrasonic-assisted treatment, after which the supernatants were extracted from the mixture by centrifugation to quantify free and esterified phenolics in the aqueous phase. The remaining residue was used to analyze the insoluble-bound phenolics in the organic phase.

All three phenolic fractions were assessed using liquid chromatography-mass spectrometry (HPLC-PDA-QDa), with a focus on their peak areas at 280 and 320 nm wavelengths.

The researchers evaluated total phenolic content (TPC) using the Folin-Ciocalteu reagent test. The Total flavonoid content (TFC) was determined via a 96-well microplate method combined with an aluminum chloride (AlCl3) colorimetric assay. Antioxidant activity was then assessed at 740 nm, with results expressed in millimoles (mmol).

The scientists modeled the gastrointestinal digestion of apricot pickles. They adjusted the sample’s pH to simulate the conditions of the different parts of the digestive tract. Next, digestive enzymes were introduced, and the digested fractions were filtered. The components were then identified, and their antioxidant activity was measured.

The HPLC chromatogram revealed rosmarinic, caffeic, p-coumaric, and ferulic acids as the main phenolic compounds detected. A comparative analysis of the three samples showed an increase in the concentration of bioactive compounds from the 30-day pickling stage (30-PP) to the finished product (PP). Among all phenolics, rosmarinic acid (RA) was the most predominant in all the samples. Ferulic acid was detected in smaller concentrations, particularly in esterified fractions that also showed higher levels of caffeic and p-coumaric acids. The lower concentration of ferulic acid is likely due to its low water solubility.

In the simulated in vitro digestion, differences between salt pickling (SP) and salt pickling with perilla (PP) were analyzed at various stages: before digestion (G0), 60 minutes after gastric digestion (G60), at the transition to intestinal digestion after 60 minutes (G60), and 120 min into small intestinal digestion (I120).

The highest release of bioactive compounds occurred at G0, while the lowest levels were observed at G60. However, a significant increase in bioactive compound release was noted between G60 and I120. At I120, both SP and PP showed the highest release of TPC and TFC. These results can be attributed to the influence of pH conditions and intestinal enzymes, which likely enhanced TPC and TFC levels.

For antioxidant activity, PP demonstrated a higher potential throughout the in vitro gastrointestinal digestion from 1 hour of gastric digestion through the final stage of small intestinal digestion, in contrast, SP exhibited a more steady trend. Overall, the acidic conditions during pickling appear to enhance the availability of RA and other perilla compounds.

“The health benefits of Japanese apricot pickles are mainly derived from the antioxidant compounds of the red perilla, which acts as both a natural food color and a health-promoting ingredient,” concludes the lead researcher Jutalak Suwannachot.

About Professor Yukiharu Ogawa
Yukiharu Ogawa is a Professor at the Graduate School and Faculty of Horticulture, Chiba University, Japan. He has previously held positions at National Food Research Institute in Tsukuba, the USDA Western Regional Research Center in California as a post-doctoral fellow, and at Kurashiki-Sakuyo University as a lecturer. The main area of his scientific work is agricultural and food process engineering, with a specific focus on food quality evaluation. His work aims to understand the impact of postharvest, food processing, and cooking procedures on the digestibility of processed food materials using simulated in vitro digestion techniques. He is also involved in developing long-term storage methods for fresh-cut vegetables and fruits monitored using atmospheric and lighting parameters.

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Journal

Food Research International

DOI

10.1016/j.foodres.2024.114752 

Method of Research

Experimental study

Subject of Research

Not applicable

Article Title

Changes in polyphenolic compounds and antioxidant activity of Japanese pickled apricot with salted red perilla leaf during pickling and digestion process

 

Artificial intelligence may enhance patient safety, say BU researchers

Study marks an important first step towards leveraging AI technology to reduce preventable harms, achieve better healthcare outcomes

Boston University School of Medicine

(Boston) — Generative artificial intelligence (genAI) uses hundreds of millions, sometimes billions, of data points to train itself to produce realistic and innovative outputs that can mimic human-created content. Its applications include personalized recommendations for online shoppers, creating audio and visual content and accelerating engineering design. In healthcare, possible genAI uses include enhancing imaging technologies, predicting the course of a disease in an individual patient and discovering new vaccines.

BU researchers tested an advanced publicly available genAI model, GPT-4, to determine its ability to answer questions across five key areas of patient safety in the 50-question self-assessment for the Certified Professional in Patient Safety (CPPS) exam, a standardized multiple-choice certification exam for patient safety professionals. GPT-4 answered 88% of the questions correctly, demonstrating a high level of performance.

“While other studies have looked at genAI's performance on exams from different healthcare specialties over the past year, ours is the first robust test of its proficiency specifically in patient safety,” said corresponding author Nicholas Cordella, MD, MSc, assistant professor of medicine at BU Chobanian & Avedisian School of Medicine. 

James Moses, MD, MPH, formerly an associate professor of pediatrics at the school and now chief  of quality, safety and patient experience at Corewell Health in Michigan, is a co-author of the study.

The researchers presented questions from the CPPS self-assessment exam to the GPT-4 model without any additional training or medical fine-tuning. They then evaluated the model's performance across various exam categories. They found GPT-4 performed particularly well in the domains of Patient Safety and Solutions, Measuring and Improving Performance, and Systems Thinking and Design/Human Factors. Based on the strength of those results, the researchers outlined areas where patient safety professionals could begin to conduct more testing of the real-world strengths and weaknesses of AI.

“Our findings suggest that AI could help doctors better recognize, address and prevent mistakes in hospitals and clinics. While more research is needed to fully understand what current AI can do in patient safety, this study shows that AI has some potential to improve healthcare by assisting clinicians in addressing preventable harms,” said Cordella who also is medical director for quality and patient safety at Boston Medical Center.

He believed the use of AI holds promise for improving patient safety systems and better tackling the intractable problem of medical errors, which are estimated to cause approximately 400,000 deaths every year.

Cordella said the study aligns with the broader idea that AI can help professionals, including doctors, enhance their work. By using AI to support their tasks, clinicians may be able to improve the safety and efficiency of healthcare, similar to how other knowledge workers are adapting AI to boost their performance.

The study also revealed limitations in current AI technology and cautioned that users must remain vigilant for bias, false confidence, fabricated data or hallucinations in large language model (like GPT-4) responses.

"Our findings suggest that AI has the potential to significantly enhance patient safety, marking an enabling step towards leveraging this technology to reduce preventable harms and achieve better healthcare outcomes. However, it's important to recognize this as an initial step, and we must rigorously test and refine AI applications to truly benefit patient care," said Cordella.

These findings appear online in the Joint Commission Journal on Quality and Patient Safety.

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DOI

10.1016/j.jcjq.2024.05.007 

Method of Research

Computational simulation/modeling

Subject of Research

Not applicable

Article Title

Artificial Intelligence and the Practice of Patient Safety: GPT-4 Performance on a Standardized Test of Safety Knowledge

Article Publication Date

25-Sep-2024

 

Alarming surge: Global crisis of childhood overweight and obesity

Researchers ‘sound the alarm’ in commentary

Florida Atlantic University

 

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The deleterious consequences of this epidemic are already evident: childhood hypertension, type 2 diabetes, among others. Researchers sound the alarm and discuss both challenges and potential solutions. 

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Credit: Alex Dolce, Florida Atlantic University

Since 1990, the rise in childhood overweight and obesity has surged across every continent, almost doubling in prevalence. While the United States has the highest prevalence, other nations are not far behind.

In Southern Europe, including Greece, Italy and Spain, 10 to 15% of children are obese, while Eastern European countries have somewhat lower rates, but are experiencing a rapid increase that may soon match Southern Europe. Globally, Asia has nearly half of all overweight children under the age of 5, and Africa has one-quarter of such children. In Latin America, about 20% of children under 20 are overweight. Many developing countries face the dual challenge of both overweight/obesity and malnutrition in their children.

The deleterious consequences of this epidemic are already evident: childhood hypertension, type 2 diabetes, among others. Researchers from Florida Atlantic University’s Schmidt College of Medicine and colleagues sound the alarm and discuss both challenges and potential solutions in a commentary published in The Maternal and Child Health Journal.

“Pediatric overweight and obesity have reached epidemic levels in the U.S. and are becoming a pandemic globally. These conditions lead to high blood pressure, type 2 diabetes and lipid disorders, which contribute to metabolic syndrome. In adults, these issues significantly increase the risks of heart attacks, stroke, liver disease, obstructive sleep apnea, arthritis and certain cancers – many of which are now occurring at younger ages,” said Charles H. Hennekens, M.D., first author and the first Sir Richard Doll Professor of Medicine and Preventive Medicine, FAU Schmidt College of Medicine. “Through coordinated clinical and public health efforts, we can address these troubling trends and work toward a healthier future for children and families globally.”

In the commentary, the authors report on the leading causes of this epidemic including high body mass index (BMI), which increases the risks of many serious health issues. In the U.S., a preschooler is considered overweight if their BMI exceeds the 85th percentile. Research shows that these children are at a significantly higher risk of being overweight during adolescence compared to those with a BMI at the 50th percentile. This underscores the misconception that children simply “outgrow” overweight issues.

In addition, the authors note that health care providers and public health practitioners face major challenges in boosting daily physical activity among children, which is crucial for increasing metabolic rates, lowering BMI, and reducing future risks of coronary heart disease.

“With declining physical education in schools and excessive time spent on electronic devices, many children fail to meet recommended activity guidelines. This sedentary behavior contributes to overweight and obesity through poor diet, reduced sleep, and decreased physical activity,” said Panagiota “Yiota” Kitsantas, Ph.D., co-author and professor and chair, FAU Department of Population Health and Social Medicine, Schmidt College of Medicine. “Encouraging organized, enjoyable activities rather than competitive ones can help children achieve necessary physical activity levels.”

The authors also caution that while increasing levels of daily physical activity is necessary, it isn’t sufficient to make a major impact on the rates of childhood overweight and obesity. The rise of high sugar containing foods, along with consumption of ultra-processed foods also are major contributors.

“Nearly 70% of the average U.S.-based child’s diet is made up of ultra-processed foods,” said Hennekens. “Moreover, consumption of ultra-processed foods among children under 24 months is rising worldwide, triggering not only the potential of developing obesity but also decreased immunological protection.”

The authors say that more research is needed to pinpoint which components of ultra-processed foods contribute to weight gain in children. However, they warn that a diet high in ultra-processed foods is linked to rising rates of overweight and obesity, with schools being a major source of these foods.

“Evidence suggests that enhancing school lunch nutritional standards could help reduce obesity, particularly among low-income children,” said Kitsantas. “We recommend adopting school food policies that remove ultra-processed foods from menus and promote healthier alternatives, alongside educational programs on healthy eating, despite the challenges posed by external influences on children.”

Among the challenges highlighted in the commentary is the use of social media and advertising, which significantly affect children’s food choices and behaviors that include sharing unhealthy food posts and recognizing many unhealthy food brands upon exposure. 

“Despite recommendations from the World Health Organization and public health authorities to restrict food marketing aimed at children, few countries have implemented such measures,” said Hennekens. “The effectiveness of existing regulations in today’s media landscape is uncertain, creating an opportunity for health providers and public health practitioners to educate families about the impact of this advertising.”

The authors explain that addressing the rising pediatric obesity epidemic requires a multifaceted approach. In 2023, the American Academy of Pediatrics endorsed WHO guidelines and released their own recommendations for managing pediatric overweight and obesity. These guidelines advise health care providers and public health practitioners to tackle social determinants of health, use motivational interviewing to modify nutrition and activity behaviors, and consider pharmacotherapy or surgery to meet personalized patient goals.

However, the authors say that while there are approved drug therapies available, before prescribing pharmacologic options, maternal and child health care providers should employ therapeutic lifestyle changes.

“While the ultimate goal is prevention of pediatric overweight and obesity as well as metabolic syndrome, to paraphrase Voltaire, we should not ‘let the perfect be the enemy of the good,’” said Hennekens.

In conclusion, the authors urge leveraging all available resources to at least stabilize the rising rates of childhood obesity and its associated health issues. Ignoring these challenges could lead to an unprecedented global epidemic of childhood and adolescent obesity, with severe future health consequences, as seen in the U.S.

“Health care and public health professionals must collaborate across disciplines to address these issues with patients, families, communities and policymakers. United efforts can help reverse these troubling trends and ensure a healthier future for children worldwide,” said Kitsantas.

Other co-authors are Dawn Harris Sherling, M.D.; Allison H. Ferris, M.D., associate professor and chair, Department of Medicine, FAU Schmidt College of Medicine; Alicia Caceres, a fourth-year premedical student at Tufts University; Katerina Benson, a third-year pre-medical student at FAU; Alexandra Rubenstein, a second-year medical student at Tufts University School of Medicine; and Sarah K. Wood, M.D., director, Harvard Macy Institute, Harvard Medical School, and former interim dean and professor and chair of maternal and child health, FAU Schmidt College of Medicine.

- FAU -

About the Charles E. Schmidt College of Medicine:

FAU’s Charles E. Schmidt College of Medicine is one of approximately 157 accredited medical schools in the U.S. The college was launched in 2010, when the Florida Board of Governors made a landmark decision authorizing FAU to award the M.D. degree. After receiving approval from the Florida legislature and the governor, it became the 134th allopathic medical school in North America. With more than 70 full and part-time faculty and more than 1,300 affiliate faculty, the college matriculates 64 medical students each year and has been nationally recognized for its innovative curriculum. To further FAU’s commitment to increase much needed medical residency positions in Palm Beach County and to ensure that the region will continue to have an adequate and well-trained physician workforce, the FAU Charles E. Schmidt College of Medicine Consortium for Graduate Medical Education (GME) was formed in fall 2011 with five leading hospitals in Palm Beach County. The Consortium currently has five Accreditation Council for Graduate Medical Education (ACGME) accredited residencies including internal medicine, surgery, emergency medicine, psychiatry, and neurology.

 

About Florida Atlantic University:
Florida Atlantic University, established in 1961, officially opened its doors in 1964 as the fifth public university in Florida. Today, the University serves more than 30,000 undergraduate and graduate students across six campuses located along the southeast Florida coast. In recent years, the University has doubled its research expenditures and outpaced its peers in student achievement rates. Through the coexistence of access and excellence, FAU embodies an innovative model where traditional achievement gaps vanish. FAU is designated a Hispanic-serving institution, ranked as a top public university by U.S. News & World Report and a High Research Activity institution by the Carnegie Foundation for the Advancement of Teaching. For more information, visit www.fau.edu.

 

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Journal

Maternal and Child Health Journal

DOI

10.1007/s10995-024-04001-6 

Method of Research

Commentary/editorial

Subject of Research

People

Article Title

Navigating the Global Pandemic in Pediatric Overweight and Obesity: Emerging Challenges and Proposed Solutions

Article Publication Date

24-Sep-2024

 

Statement by NIH on research misconduct findings

NIH/Office of the Director

 

Following an investigation, the National Institutes of Health (NIH) has made findings of research misconduct against Eliezer Masliah, M.D., due to falsification and/or fabrication involving re-use and relabel of figure panels representing different experimental results in two publications. NIH will notify the two journals of its findings so that appropriate action can be taken. NIH initiated its research misconduct review process in May 2023 after it received allegations from the HHS Office of Research Integrity (ORI) that same month. NIH began its investigation phase in December 2023, concluded its investigation of these allegations on September 15, 2024, and notified HHS ORI of its findings.

 

Dr. Masliah joined the agency in the summer of 2016 as director of the Division of Neuroscience (DN) at the National Institute on Aging and an NIH intramural researcher investigating synaptic damage in neurodegenerative disorders. He has published numerous papers as an NIH intramural researcher. Currently, Dr. Masliah is not serving in the capacity of director of the NIA DN. Amy Kelley, M.D., NIA Deputy Director, is also serving as the Acting NIA DN Director. Beyond this information, NIH does not discuss personnel matters.

 

Any allegations involving Dr. Masliah’s NIH-supported extramural research prior to joining NIH would be referred to HHS ORI, consistent with NIH policies and procedures.  

 

Satellite data fusion enhances the early detection of convective clouds

Institute of Atmospheric Physics, Chinese Academy of Sciences

 

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Developing cumulus clouds

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Credit: Yang Gao

As global warming continues, convective weather events are becoming more frequent. The early stage of these storms, known as convective initiation (CI), can be monitored using geostationary satellites. However, detecting CI accurately remains a challenge. The current detection methods still have a high rate of false alarms and missed events. One key reason for this is that the resolution of existing geostationary meteorological satellites is not yet precise enough to meet the requirements for better detection.

To address this issue, researchers from the National Satellite Meteorological Center in China have proposed a new fusion method in which the high-resolution texture information provided by Gaofen-4 (GF-4), an Earth observation satellite, is combined with the multispectral data of Fengyun-4A (FY-4A), a geostationary meteorological satellite. This approach retains the detection advantages of each satellite, ensuring the accuracy of spectral information, while fully considering the early growth patterns of convective clouds, thereby significantly enhancing the capability to detect CI. The results have recently been published in Atmospheric and Oceanic Science Letters.

The fused data revealed a notable improvement in the detection of smaller-scale convective clouds, which often develop rapidly and can be difficult to capture using traditional observation methods. By leveraging high-resolution Earth observation satellites, forecasters gain the ability to identify these clouds much earlier in their formation.

"This early detection is particularly important as small convective clouds can quickly evolve into severe weather systems, such as thunderstorms or localized heavy rainfall", says the corresponding author, Prof. Xin Wang. "The integration of these detailed satellite data helps improve the timing of forecasts, allowing meteorologists to track cloud development with greater accuracy and issue earlier, more reliable warnings".

Beyond simply detecting clouds earlier, the integration of high-resolution satellite data enhances the precision of identifying where these clouds will form and intensify. This added spatial accuracy is crucial in understanding localized weather patterns, which may have been previously overlooked.

"For decision-makers, this means not only having a more detailed picture of the early stages of convective cloud development, but also having access to data that informs strategic responses," says Yang Gao, one of the other authors of the paper.

By pinpointing the exact locations of potential severe weather systems, this advanced detection method enables more targeted and efficient disaster preparedness and mitigation efforts.

"Ultimately, it improves our ability to safeguard communities from the impacts of extreme weather," Professor Wang concludes.

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Journal

Atmospheric and Oceanic Science Letters

DOI

10.1016/j.aosl.2024.100551 

Who lives in the treetops? DNA-collecting drone provides insights

American Chemical Society

 

video: 

This drone can release a specialized probe in the rainforest to collect environmental DNA from the trees’ branches and leaves.

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Credit: Adapted from Environmental Science & Technology 2024, DOI: 10.1021/acs.est.4c05595

Squinting into the treetops won’t reveal the tiny organisms up there. But these creatures leave clues, in the form of DNA, on the leaves and branches. Now, researchers report in ACS’ Environmental Science & Technology that they have developed a way to collect this genetic material: a drone with a specialized fabric probe. The team flew the drone above the rainforest and, based on DNA collected by the probe, identified the invertebrates in the canopy.

“If we want people to protect nature, we need to tell them what we are actually protecting — with our solution, we hope to better understand the life in the canopy,” says the study’s lead author, Steffen Kirchgeorg.

Drones go where people can’t or shouldn’t go, including remote, protected or inaccessible locations. So, researchers have started to use aerial robots to take pictures, deploy sensors and collect samples in the forest canopy. To identify the species living in and around trees, samples are taken of genetic material left on leaves and branches. This environmental DNA (eDNA) comes from mucus, feces and dead skin cells. However, if a drone outfitted with swabs to gently collect eDNA accidentally collides with a tree, both the robot and plant can be damaged. So, Kirchgeorg, Stefano Mintchev and colleagues wanted to design a sampling system that keeps the drone out of the vegetation.

They developed a drone sampling system with a specialized fabric probe that brushes against branches and leaves to collect eDNA. When a remote pilot activates a pully underneath the drone, a tether lowers and raises the probe through the canopy. The system includes a piece of fleece cloth cut into a circle, similar in shape to a coffee filter, with strips of fiberglass attached to provide structure. In addition, a sensor keeps the probe’s tether from tangling on branches: If it detects an impact, the researchers programmed the system to automatically shift position before completing the drop or retrieval.

In proof-of-concept demonstrations, the researchers flew their drone into a rainforest in Southeast Asia, sending it beyond their line of sight to retrieve genetic material from trees 10 different times. When the drone returned from the flights, the researchers removed the fabric and extracted eDNA from each probe before bringing the samples to a lab for analysis and species identification. Across the 10 separate samples, most of the organisms detected were arachnids and insects. Additional species of note, according to the researchers, include the long-tailed macaque (monkey), multiple ant and termite species, and a type of fly called the gall midge. The study presents another way to study biodiversity in remote habitats, which the researchers say is critical for conservation and restoration initiatives.

The authors acknowledge funding from the Swiss National Science Foundation through an Eccellenza Grant and a Bridge Discovery Grant, the European Research Council through the European Union’s Horizon 2020 research and innovation program, Rütli-Stiftung, the ETH Foundation, the XPRIZE Foundation, and the Alana Foundation.

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The American Chemical Society (ACS) is a nonprofit organization chartered by the U.S. Congress. ACS’ mission is to advance the broader chemistry enterprise and its practitioners for the benefit of Earth and all its people. The Society is a global leader in promoting excellence in science education and providing access to chemistry-related information and research through its multiple research solutions, peer-reviewed journals, scientific conferences, e-books and weekly news periodical Chemical & Engineering News. ACS journals are among the most cited, most trusted and most read within the scientific literature; however, ACS itself does not conduct chemical research. As a leader in scientific information solutions, its CAS division partners with global innovators to accelerate breakthroughs by curating, connecting and analyzing the world’s scientific knowledge. ACS’ main offices are in Washington, D.C., and Columbus, Ohio.

Registered journalists can subscribe to the ACS journalist news portal on EurekAlert! to access embargoed and public science press releases. For media inquiries, contact newsroom@acs.org.

Note: ACS does not conduct research but publishes and publicizes peer-reviewed scientific studies.

Follow us: X, formerly Twitter | Facebook | LinkedIn | Instagram

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Journal

Environmental Science & Technology

DOI

10.1021/acs.est.4c05595 

Article Title

“eProbe: Sampling of Environmental DNA within Tree Canopies with Drones”

Using satellite images to understand changes in river flow

Journal of Remote Sensing

Since 1953, an equation has been used to explain the relationship between hydraulic parameters (river width, depth, and velocity) and river discharge, the volume of water that flows through a river channel. This relationship is called at-a-station hydraulic geometry (AHG). Understanding this relationship is important for hydraulic engineering, predicting flooding, navigation, and more.

Though this equation has been studied extensively through field research, this has limited sample size and study areas and is not enough to fully explain the variety of factors beyond hydraulic parameters that impact river discharge. The ability to use satellites to monitor the surface of the earth through remote sensing may provide an option to expand the way AHG is understood. The data was presented in a paper published in the Journal of Remote Sensing.

“Understanding the response of width to changing discharge in different rivers is crucial for hydraulic modeling and river management. However, previous research is limited in spatiotemporal coverage by field measurements and only offers a fragmentary understanding in confined areas. This study introduces new data sources—multi-temporal river width data derived from Landsat and global discharge observations built upon years of progresses by the community —to provide a more comprehensive scope,” said Zimin Yuan, a researcher at the Institute of Remote Sensing and Geographic Information Systems at Peking University in Beijing, China. By using several data sets covering years ranging from 1979 to 2020 and matching data against Google Map Images to find limitations, researchers were able to derive an unprecedented global samples of AHG.

18 variables relating to AHG were identified from a vast range of factors, which could be divided into six categories that included: hydrology, physiography, climate, land cover, geology, and human influences. To focus the list of variables, researchers looked at the relationship between each variable and how it impacts the AHG. Then, because AHG is also closely related to the planform river pattern, researchers also factored in whether a river was meandering, anabranching, straight, or braided.

Understanding how these identified factors are related is essential for predicting river width changes, which can have impacts on the land and communities around the river. “We found that a 1% increase in discharge will result in a median of 0.2% increase in river width worldwide. Reaches characterized by cohesive soil, high forest coverage, and less human influences typically exhibit weaker response of width to discharge changes. River planform patterns are correlated with width response, and the relationship can be well correlated by climatic conditions,” said Yuan.

Looking ahead, researchers are hoping to understand the changes in river width over time, which is impacted by channel shape. They also plan to explore the abilities of remote sensing to find other factors that influence river discharge. “We plan to explore the depth response to discharge using remote sensing in the future. We also hope to reconstruct global channel shape on the basis of the samples and knowledge obtained in this and following studies,” said Yuan.

Other contributors include Peirong Lin of the Institute of Remote Sensing and Geographic Information Systems at Peking University; Xiwei Guo at the Department of Geosciences at The Pennsylvania State University; Kai Zhang at Geovis Environment Technology Co. Ltd.; and Hylke E. Beck at the Physical Science and Engineering Division of King Abdullah University of Science and Technology.

The National Natural Science Foundation of China, the Beijing Nova Program, and the Yunnan Provincial Science and Technology Project at Southwest United Graduate School funded this research. 

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Journal

Journal of Remote Sensing

DOI

10.34133/remotesensing.0271 

Method of Research

Imaging analysis

Subject of Research

Not applicable

Article Title

Revisiting At-a-Station Hydraulic Geometry Using Discharge Observations and Satellite-Derived River Widths