Saturday, December 10, 2022

Astronomers report most distant known galaxies, detected and confirmed by JWST

Spectroscopic observations with JWST confirm four early galaxies dating back to less than 400 million years after the Big Bang, three of which are the most distant confirmed to date

Peer-Reviewed Publication

UNIVERSITY OF CALIFORNIA - SANTA CRUZ

Images and spectra of early galaxies 

IMAGE: THE JWST ADVANCED DEEP EXTRAGALACTIC SURVEY (JADES) FOCUSED ON THE AREA IN AND AROUND THE HUBBLE SPACE TELESCOPE’S ULTRA DEEP FIELD. USING WEBB’S NIRCAM INSTRUMENT, SCIENTISTS OBSERVED THE FIELD IN NINE DIFFERENT INFRARED WAVELENGTH RANGES. FROM THESE IMAGES (SHOWN AT LEFT), THE TEAM SEARCHED FOR FAINT GALAXIES THAT ARE VISIBLE IN THE INFRARED BUT WHOSE SPECTRA ABRUPTLY CUT OFF AT A CRITICAL WAVELENGTH KNOWN AS THE LYMAN BREAK. WEBB’S NIRSPEC INSTRUMENT THEN YIELDED A PRECISE MEASUREMENT OF EACH GALAXY’S REDSHIFT (SHOWN AT RIGHT). FOUR OF THE GALAXIES STUDIED ARE PARTICULARLY SPECIAL, AS THEY WERE REVEALED TO BE AT AN UNPRECEDENTEDLY EARLY EPOCH. THESE GALAXIES DATE BACK TO LESS THAN 400 MILLION YEARS AFTER THE BIG BANG, WHEN THE UNIVERSE WAS ONLY 2% OF ITS CURRENT AGE. IN THE BACKGROUND IMAGE BLUE REPRESENTS LIGHT AT 1.15 MICRONS (115W), GREEN IS 2.0 MICRONS (200W), AND RED IS 4.44 MICRONS (444W). IN THE CUTOUT IMAGES BLUE IS A COMBINATION OF 0.9 AND 1.15 MICRONS (090W+115W), GREEN IS 1.5 AND 2.0 MICRONS (150W+200W), AND RED IS 2.0, 2.77, AND 4.44 MICRONS (200W+277W+444W). view more 

CREDIT: IMAGE: NASA, ESA, CSA, M. ZAMANI (ESA/WEBB), LEAH HUSTAK (STSCI) SCIENCE: BRANT ROBERTSON (UC SANTA CRUZ), S. TACCHELLA (CAMBRIDGE), E. CURTIS-LAKE (UOH), S. CARNIANI (SCUOLA NORMALE SUPERIORE), JADES COLLABORATION

An international team of astronomers has discovered the earliest and most distant galaxies confirmed to date using data from the James Webb Space Telescope (JWST). The telescope captured light emitted by these galaxies more than 13.4 billion years ago, which means the galaxies date back to less than 400 million years after the Big Bang, when the universe was only 2% of its current age.

Initial observations from JWST yielded several candidate galaxies at extreme distances, as had earlier observations with the Hubble Space Telescope. Now, four of these targets have been confirmed by obtaining long spectroscopic observations, which not only provide secure measurements of their distances, but also allow astronomers to characterize the physical properties of the galaxies.

“We’ve discovered galaxies at fantastically early times in the distant universe," said Brant Robertson, professor of astronomy and astrophysics at UC Santa Cruz. “With JWST, for the first time we can now find such distant galaxies and then confirm spectroscopically that they really are that far away.”

Astronomers measure the distance to a galaxy by determining its redshift. Due to the expansion of the universe, distant objects appear to be receding from us and their light is stretched to longer, redder wavelengths by the Doppler effect. Photometric techniques based on images captured through different filters can provide redshift estimates, but definitive measurements require spectroscopy, which separates the light from an object into its component wavelengths.

The new findings focus on four galaxies with redshifts higher than 10. Two galaxies initially observed by Hubble now have confirmed redshifts of 10.38 and 11.58. The two most distant galaxies, both detected in JWST images, have redshifts of 13.20 and 12.63, making them the most distant galaxies confirmed by spectroscopy to date. A redshift of 13.2 corresponds to about 13.5 billion years ago.

“These are well beyond what we could have imagined finding before JWST,” Robertson said. “At redshift 13, the universe is only about 325 million years old.”

Robertson and Emma Curtis-Lake from the University of Hertfordshire (U.K.) will be presenting the new findings on December 12 at a Space Telescope Science Institute (STScI) conference in Baltimore on “First Science Results from JWST.” They are the lead authors of two papers on the results that have not yet been through the peer-review process (see links below).

The observations result from a collaboration of scientists who led the development of two of the instruments onboard Webb, the Near-Infrared Camera (NIRCam) and the Near-Infrared Spectrograph (NIRSpec). The investigation of the faintest and earliest galaxies was the leading motivation in the concepts for these instruments. In 2015, the instrument teams joined together to propose the JWST Advanced Deep Extragalactic Survey (JADES), an ambitious program that has been allocated just over one month of the telescope’s time and is designed to provide a view of the early universe unprecedented in both depth and detail. JADES is an international collaboration of more than eighty astronomers from ten countries.

“These results are the culmination of why the NIRCam and NIRSpec teams joined together to execute this observing program,” said Marcia Rieke, NIRCam principal investigator at the University of Arizona.

The JADES program began with NIRCam, using over 10 days of mission time to observe a small patch of sky in and around the Hubble Ultra Deep Field. Astronomers have been studying this region for over 20 years with nearly all large telescopes. The JADES team observed the field in nine different infrared wavelength ranges, capturing exquisite images that reveal nearly 100,000 distant galaxies, each billions of light years away.

The team then used the NIRSpec spectrograph for a single three-day observation period to collect the light from 250 faint galaxies. This yielded precise redshift measurements and revealed the properties of the gas and stars in these galaxies.

“With these measurements, we can know the intrinsic brightness of the galaxies and figure out how many stars they have,” Robertson said. “Now we can start to really pick apart how galaxies are put together over time.”

Coauthor Sandro Tacchella from the University of Cambridge in the United Kingdom added, “It is hard to understand galaxies without understanding the initial periods of their development. Much as with humans, so much of what happens later depends on the impact of these early generations of stars. So many questions about galaxies have been waiting for the transformative opportunity of Webb, and we’re thrilled to be able to play a part in revealing this story.”

According to Robertson, star formation in these early galaxies would have begun about 100 million years earlier than the age at which they were observed, pushing the formation of the earliest stars back to around 225 million years after the Big Bang.

“We are seeing evidence of star formation about as early as we could expect based on our models of galaxy formation,” he said.

Other teams have identified candidate galaxies at even higher redshifts based on photometric analyses of JWST images, but these have yet to be confirmed by spectroscopy. JADES will continue in 2023 with a detailed study of another field, this one centered on the iconic Hubble Deep Field, and then a return to the Ultra Deep Field for another round of deep imaging and spectroscopy. Many more candidates in the field await spectroscopic investigation, with hundreds of hours of additional time already approved.

The following papers on the new findings have been submitted for publication and are available online:

“Discovery and properties of the earliest galaxies with confirmed distances” (https://arxiv.org/abs/2212.04480)

“Spectroscopy of four metal-poor galaxies beyond redshift ten” (https://t.co/QIy5bDmqwL)

BioRescue produces primordial germ cells from northern white rhino stem cells – a world’s first for large mammals

Creation of primordial germ cell-like cells (PGCLSs) from induced pluripotent stem cells of the northern white rhino Nabire nourishes the hope to prevent the extinction of the northern white rhino

Peer-Reviewed Publication

LEIBNIZ INSTITUTE FOR ZOO AND WILDLIFE RESEARCH (IZW)

Northern White Rhino Nabire at Safari Park Dvur Kralove 

IMAGE: NORTHERN WHITE RHINO NABIRE, FROM WHICH THE PRIMORDIAL GERM CELLS HAVE BEEN PRODUCED, AT SAFARI PARK DVUR KRALOVE view more 

CREDIT: PHOTO BY KHALIL BAALBAKI

In its race to advance assisted reproduction and stem cell associated technologies to save the northern white rhinoceros from extinction, the BioRescue consortium announces a major breakthrough: the creation of primordial germ cell-like cells (PGCLSs) from induced pluripotent stem cells of the northern white rhino Nabire. This milestone was led by specialists from Osaka University, Japan, and has never been achieved in large mammals before. Now there is one last step to master for the production of artificial rhino gametes (eggs and sperm) from preserved tissue. If successful, this would boost the availability and genetic diversity of embryos and become a cornerstone for saving the northern white rhinoceros. The scientists describe the culture systems and processes for the induction of the PGCLCs from stem cells in a newly published paper in the journal Science Advances.

Thirty-three-year-old Najin and her daughter Fatu are the last surviving northern white rhinos on the planet. Without a possibility for natural reproduction with only two females left of their kind, the only hope for the northern white rhinoceros lies in advanced assisted reproduction technologies. The scientists of the BioRescue consortium have already produced northern white rhino embryos by in vitro fertilisation of oocytes with sperm. Embryos are destined to be carried to term by southern white rhino surrogate mothers. Crucial to embryo production is the availability of oocytes (egg cells) and sperm. Female Fatu is the only donor of natural oocytes left and frozen sperm is available to the program from only four males – and some of these males are closely related to Fatu. With stem cell associated techniques (SCAT) the BioRescue scientists aim to overcome this bottleneck: By using stored and preserved tissue of rhino skin it should be possible in principle to create induced pluripotent stem cells (iPCSs), primordial germ cells and finally artificial gametes. This could increase the number of individuals in the founder group of a future population to 12 and permit the production of oocytes in much larger quantities. According to their most recent paper published in the journal Science Advances, the team has now successfully cultivated primordial germ cells (PGCs) – the precursors of eggs and sperm – from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

In sexually reproducing organisms such as humans, rhinos or mice, primordial germ In sexually reproducing organisms such as humans, rhinos or mice, primordial germ cells (PGCs) are embryonic precursors of sperm and eggs that pass on genetic and epigenetic information from one generation to the next. In order for them to be developed from stem cells, they need a very specific environment in which signals from hormones or proteins trigger the required morphological and functional transformation. This also includes a migration of the cells in the body (PGC migration). For the first time in large mammals, BioRescue scientists Masafumi Hayashi and Katsuhiko Hayashi and their team from Osaka University succeeded in creating such an environment in a culture system. They established culture systems for the southern white rhino, for which embryonic stem cells are available, and the northern white rhino, for which they used induced pluripotent stem cells derived from tissue samples. In order to succeed they needed to identify the signals which had to be introduced to the system at specific time points and the order in which they should trigger the development into PGCLCs.

The scientists relied on knowledge from the mouse model: In 2016, Katsuhiko Hayashi and his team managed to create primordial germ cell-like cells and finally germ cells from mice that were fertilised in the lab and resulted in healthy offspring being born. In the case of the white rhinoceroses, Hayashi is working in close cooperation within BioRescue with Sebastian Diecke’s Pluripotent Stem Cells Platform at the Max Delbrück Center and with reproduction experts Thomas Hildebrandt from Leibniz-IZW, both of them last authors of the paper, and Cesare Galli from Avantea.

Through repeated refinement, the BioRescue team produced PGCLCs from northern white rhino Nabire under a defined condition. Because PGCs are the founder population for gametes, this accomplishment paves a way to produce functional gametes from induced pluripotent stem cells from northern white rhinos which will contribute to the effort to rewind their extinction. As soon as the creation of artificial gametes is successful, this plan merges with the procedures that BioRescue carries out with natural gametes: Just like with oocytes obtained from Fatu and sperm thawed from frozen samples, the artificially created eggs and sperm would be in vitro fertilised in the lab. The embryos created in this way would be stored safely in liquid nitrogen until a transfer to a surrogate mother is possible.

The southern white rhino embryonic stem cells which were used in Japan come from the Avantea laboratory in Cremona, Italy, where they were grown by Cesare Galli’s team. The newly derived northern white rhino PGCs, meanwhile, originated from the skin cells of Fatu’s aunt Nabire, who died in 2015 at Safari Park Dvůr Králové in the Czech Republic. Sebastian Diecke’s team at the Max Delbrück Center was responsible for converting them into induced pluripotent stem cells.

All BioRescue procedures are accompanied by ethical risk assessments, conducted under the guidance of the Ethics Laboratory for Veterinary Medicine, Conservation and Animal Welfare at the University of Padua, Italy


Northern White Rhinos Najin and Fatu in their enclosure at Ol Pejeta Conservancy (Kenya). They are are the only two remaining individuals of their kind on the planet.

CREDIT

Photo by Jan Zwilling



Immune system of modern Papuans shaped by DNA from ancient Denisovans


Sequences of Denisovan DNA are located near immune-related genes and regulate their activity

Peer-Reviewed Publication

PLOS

Modern Papuans’ immune system likely evolved with a little help from the Denisovans, a mysterious human ancestor who interbred with ancient humans, according to a new study led by Irene Gallego Romero of the University of Melbourne, Australia, publishing December 8, 2022 in the open access journal PLOS Genetics.

Papuans, the indigenous peoples of New Guinea Island, owe up to 5% of their genome to Denisovans, an extinct group closely related to Neanderthals who are known only by their DNA and sparse remains in Siberia and Tibet. To better understand the significance of this genetic contribution, researchers searched the genomes of 56 Papuan individuals to see if they carried Denisovan or Neanderthal DNA sequences, and then predicted how those sequences might affect the functioning of different types of cells.

Based on the location of the non-human sequences, the team found that in Papuans, Denisovan DNA – but not Neanderthal DNA – appears to strongly and consistently affect immune cells and functions. Further testing in cell cultures confirmed that Denisovan DNA sequences successfully regulated nearby genes, turning their expression up or down in ways that could affect how people respond to infections.

The new study suggests that Denisovan DNA sequences altered the immune response in early modern humans living in New Guinea and nearby islands, potentially helping them adapt to their local environment. The researchers conclude that further exploring how DNA from extinct human ancestors affects gene expression may be the key to understanding the consequences of interbreeding between ancient humans and other groups. The results also support the idea that archaic DNA has had a broad impact in shaping the genetic diversity and evolution of modern humans, and has likely affected multiple characteristics in people who inherited Denisovan and Neanderthal DNA.

Dr. Davide Vespasiani, first author, adds, “We show that not only Neanderthal, but also Denisovan DNA is very likely to contribute to gene expression in human populations. Further validations will reveal whether these effects are mostly cell type specific or consistent across cells.”

Dr. Irene Gallego Romero, senior author, concludes, “Some of the Denisovan DNA that has persisted in Papuan individuals until today plays a role in regulating genes involved in the immune system. Our study is the first to comprehensively shed light on the functional legacy of Denisovan DNA in the genomes of present-day humans.”

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In your coverage, please use this URL to provide access to the freely available article in PLOS Geneticshttp://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010470  

Citation: Vespasiani DM, Jacobs GS, Cook LE, Brucato N, Leavesley M, Kinipi C, et al. (2022) Denisovan introgression has shaped the immune system of present-day Papuans. PLoS Genet 18(12): e1010470. https://doi.org/10.1371/journal.pgen.1010470

Author Countries: Australia, Estonia, France, New Zealand, Papua New Guinea, United Kingdom

Funding: This work was supported by an award from the Leakey Foundation and by Australian Research Council Discovery Project DP200101552, both to I.G.R. and by The French National Research Agency (ANR) (grant PAPUAEVOL n° ANR-20-CE12-0003-01 (F.X.R). N.B. was supported by the PAPUAEVOL grant. D.M.V was supported by the University of Melbourne’s Albert Shimmins Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

The genes of the sixth sense

Peer-Reviewed Publication

MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE IN THE HELMHOLTZ ASSOCIATION

Cells of the sixth sense 

IMAGE: DIFFERENT POPULATIONS OF SENSORY NEURONS CELL BODIES IN A DORSAL ROOT GANGLION (RIGHT) AND THEIR AXONS IN THE SPINAL CORD (LEFT): THE CELLS IN GREEN DETECT PROPRIOCEPTIVE INFORMATION WHILE THE CELLS IN RED THERMAL AND TACTILE INFORMATION. view more 

CREDIT: STEPHAN DIETRICH, ZAMPIERI LAB, MAX DELBRÜCK CENTER

To perform coordinated movements, we rely on special sensory neurons in our muscles and joints. Without them, the brain wouldn’t know what the rest of our body was doing. A team led by Niccolò Zampieri has studied their molecular markers to better understand how they work and describes the results in Nature Communications.

Sight, hearing, smell, taste, touch: We’re all familiar with the five senses that allow us to experience our surroundings. Equally important but much less well known is the sixth sense: “Its job is to collect information from the muscles and joints about our movements, our posture and our position in space, and then pass that on to our central nervous system”, says Dr. Niccolò Zampieri, head of the Development and Function of Neural Circuits Lab at the Max Delbrück Center in Berlin. “This sense, known as proprioception, is what allows the central nervous system to send the right signals through motor neurons to muscles so that we can perform a specific movement.”

This sixth sense – which, unlike the other five, is entirely unconscious – is what stops us from falling over in the dark, and what allows us to raise a cup of coffee to our mouth with our eyes shut in the morning. But that’s not all: “People without proprioception can’t actually perform coordinated movements,” says Zampieri. He and his team have now published an article in the journal “Nature Communications”, in which they describe the molecular markers of the cells involved in this sixth sense. The findings should help researchers to better understand how proprioceptive sensory neurons (pSN) work.

Precise connections are crucial

The pSN cell bodies are located in the dorsal root ganglia of the spinal cord. They are connected via long nerve fibers to the muscle spindles and Golgi tendon organs that constantly register stretch and tension in every muscle of the body. The pSN send this information to the central nervous system, where it is used to control motor neuron activity so that we can perform movements.

“One prerequisite for this is that pSN precisely connect to different muscles in our bodies,” says Dr. Stephan Dietrich, a member of Zampieri’s lab. However, almost nothing was known about the molecular programs that enable these precise connections and lend the muscle-specific pSN their unique identity. “That’s why we used our study to look for molecular markers that differentiate the pSN for the abdominal, back and limb muscles in mice,” says Dietrich, lead author of the study, which was carried out at the Max Delbrück Center.

Guidance for nascent nerve fibers

Using single-cell sequencing, the team investigated which genes in the pSN of the abdominal, back and leg muscles are read and translated into RNA. “And we did find characteristic genes for the pSN connected to each muscle group,” says Dietrich. “We also showed that these genes are already active at the embryonic stage and remain active for at least a while after birth.” Dietrich explains that this means there are fixed genetic programs that decide whether a proprioceptor will innervate the abdominal, back or limb muscles. 

Among their findings, the Berlin researchers identified several genes for ephrins and their receptors. “We know that these proteins are involved in guiding nascent nerve fibers to their target during development of the nervous system,” says Dietrich. The team found that the connections between the proprioceptors and the rear leg muscles were impaired in mice that can’t produce ephrin-A5.

One aim is better neuroprostheses

“The markers we identified should now help us further investigate the development and function of individual muscle-specific sensory networks,” says Dietrich. “With optogenetics, for instance, we can use light to turn proprioceptors on and off, either individually or in groups. This will allow us to reveal their specific role in our sixth sense,” adds Zampieri.

This knowledge should eventually benefit patients, such as those with spinal cord injuries. “Once we better understand the details of proprioception, we’ll be able to optimize the design of neuroprostheses, which take over motor or sensory abilities that have been impaired by an injury,” says Zampieri.

Altered muscle tension causes a crooked spine

He adds that researchers in Israel have recently discovered that properly functioning proprioception is also important for a healthy skeleton. Scoliosis, for instance, is a condition that sometimes develops during growth in childhood and causes the spine to become crooked and twisted. “We suspect this is caused by dysfunctional proprioception, which alters the muscle tension in the back and distorts the spine,” says Zampieri.

Hip dysplasia, an abnormality of the hip joint, might also be caused by faulty proprioception. This has led Zampieri to envision another outcome of the research: “If we can better understand our sixth sense, it will be possible to develop novel therapies that effectively counteract these and other types of skeletal damage.” 

Max Delbrück Center 

The Max Delbrück Center for Molecular Medicine in the Helmholtz Association (Max Delbrück Center) is one of the world’s leading biomedical research institutions. Max Delbrück, a Berlin native, was a Nobel laureate and one of the founders of molecular biology. At the locations in Berlin-Buch and Mitte, researchers from some 70 countries study human biology – investigating the foundations of life from its most elementary building blocks to systems-wide mechanisms. By understanding what regulates or disrupts the dynamic equilibrium of a cell, an organ, or the entire body, we can prevent diseases, diagnose them earlier, and stop their progression with tailored therapies. Patients should benefit as soon as possible from basic research discoveries. The Max Delbrück Center therefore supports spin-off creation and participates in collaborative networks. It works in close partnership with Charité – Universitätsmedizin Berlin in the jointly run Experimental and Clinical Research Center (ECRC), the Berlin Institute of Health (BIH) at Charité, and the German Center for Cardiovascular Research (DZHK). Founded in 1992, the Max Delbrück Center today employs 1,800 people and is funded 90 percent by the German federal government and 10 percent by the State of Berlin.