Pig kidney functions normally for two months in brain-dead recipient
Columbia University Irving Medical Center
NEW YORK, NY (Nov. 13, 2025)--A study of a pig kidney that flourished for two months in a brain-dead recipient shows that a protocol developed by Columbia University immunologists can help prevent long-term rejection of a xenotransplant.
In the study, surgeons at New York University Langone Health transplanted a pig kidney and the same animal’s thymus gland into a 57-year-old man with glioblastoma who had been declared brain-dead at the hospital. The study was published in Nature.
Studying xenotransplants (organs from other species) in brain-dead individuals gives researchers more extensive details about how a transplanted organ is working and how the recipient’s immune system is reacting than is possible with living patients. But until now, xenotransplant studies in decedents have been short, ending one or two weeks after the surgery.
“In our study, we obtained an unprecedented number of tissue, blood, and fluid samples from the recipient, allowing us to monitor immunological changes over time and identify ways to improve the success of xenotransplantation,” says Megan Sykes, director of the Columbia Center for Translational Immunology, one of the study’s co-leaders who developed the thymus-kidney strategy.
Pig thymus reduces rejection
Over the past three decades, Sykes and her colleagues have studied how to train a transplant recipient’s immune system to tolerate a donated organ, including xenotransplants.
In animal studies, Sykes and her team have found that transplanting tissue from a donor’s thymus—which teaches immune cells to distinguish between native and foreign tissue—along with the replacement organ reduces the immune attack on the donated organ, producing remarkable long-term results.
The approach also seemed to work in the decedent.
“Our analyses suggested that the transplanted pig thymus may have helped to restrain the recipient’s immune system from attacking the kidney.”
The thymus also may have prevented a dangerous loss of proteins from the body, which has hampered xenotransplants in living patients. “One of the kidney’s jobs is to keep proteins in the body and prevent their release into urine,” Sykes says. “With the thymo-kidney transplant, we saw no evidence of this complication.”
Attack by recipient’s immune system still a challenge
Despite the calming presence of the pig thymus, Sykes’s analyses found immunological challenges that still need to be addressed to improve long-term outcomes with xenotransplants.
One month after transplantation, a rejection episode that was thought to be caused by antibodies occurred. However, studies in the Sykes lab implicated the recipient’s own T cells that existed before the transplant in attacking the pig kidney. The rejection episode was successfully treated by temporarily eliminating the recipient’s T cells.
The researchers also found new antibodies directed against the donor organ after the transplant, but not against the pig antigens predicted to pose a problem (and which have been edited by some xenotransplant developers).
“These antibodies are directed at other unknown pig antigens, and I think it will be really important to identify them to improve future xenotransplants,” Sykes says.
Minimal gene editing required
The pig kidney transplanted in this study had been genetically edited to eliminate the alpha-gal sugar molecule on pig organs, wich causes immediate rejection when the organs are transplanted into humans.
Though some suppliers have made many other genetic modifications to pig organs to lower the risk of rejection, the minimally edited organ performed surprisingly well for two months.
“At two months, the kidney was still functioning fine with no major problems,” Sykes says. “It suggests that extensive genetic editing of the donor pig organ may not be as important as controlling the response from the patient’s pre-existing T cells in recipients who do not have high levels of antibodies before the transplant.”
Minimally edited pig organs are also easier to produce, promising a greater supply for patients than more rarefied pig organs.
Future directions
Though results from decedent studies may not apply to all xenotransplants in living recipients, there is a need to continue such studies. “Additional studies in decedents can help us improve xenotransplants,” Sykes says.
“But it is a big sacrifice on the part of the family, and they must ultimately decide when the study stops. The family of the recipient in this study was very generous. What we’ve learned has been invaluable and will help us advance the science of xenotransplantation.”
Additional information
The study, "Physiology and immunology of pig-to-human decedent kidney xenotransplant," by Robert A Montgomery and Jeffrey M. Stern, et al., was published in Nature on Nov. 13.
###
Columbia University Irving Medical Center (CUIMC) is a clinical, research, and educational campus located in New York City. Founded in 1928, CUIMC was one of the first academic medical centers established in the United States of America. CUIMC is home to four professional colleges and schools that provide global leadership in scientific research, health and medical education, and patient care including the Vagelos College of Physicians and Surgeons, the Mailman School of Public Health, the College of Dental Medicine, the School of Nursing. For more information, please visit cuimc.columbia.edu.
Journal
Nature
Subject of Research
People
Article Title
Physiology and immunology of pig-to-human decedent kidney xenotransplant
Article Publication Date
13-Nov-2025
Immune reactions found behind human rejection of transplanted pig kidneys
Targeting immune responses may save future organ transplants
NYU Langone Health / NYU Grossman School of Medicine
Researchers have uncovered and then overcome an obstacle that has led to the failure of pioneering efforts in xenotransplantation, in which an animal kidney is transplanted into a human.
More than 800,000 Americans have late-stage kidney disease yet only 3% receive a transplant each year, according to the U.S. Centers for Disease Control and Prevention. To boost the supply of available organs, experts are exploring the use of genetically modified pig kidneys. The genetic changes are meant to keep the human immune system from recognizing the animal organ as foreign, and attacking it to cause rejection. However, recipients’ immune reactions can still lead to organ damage and failure after the surgery.
To better understand the immune mechanisms behind xenotransplant rejection, a new investigation, led by NYU Langone Health researchers, explored the transplantation of a genetically engineered pig kidney into a brain-dead recipient with a beating heart and on a ventilator, whose family donated his body to science. For 61 days after the surgery, the team was able to collect samples of tissue, blood, and body fluid at a pace that is impossible to safely maintain in primates or living patients. As a result, they had a rare opportunity to trace the network of interactions that occur among immune cells when a pig organ is being tolerated by a human and when it undergoes a rejection episode.
In the first of two reports publishing online Nov. 13 in the journal Nature, the study authors created a detailed map of both human and pig kidney immune activity in response to the transplant. They found that rejection was driven by antibodies — immune proteins that “tag” foreign substances for later destruction — as well as by T cells, which target and kill specific invaders.
Once the researchers uncovered this set of reactions, they for the first time successfully reversed the rejection using a combination of drugs approved by the Food and Drug Administration to temper both the antibody and T cell activity. There was no evidence of permanent damage or reduced kidney function after the intervention.
“Our results better prepare us for anticipating and addressing harmful immune reactions during pig-organ transplantation in living humans,” said study lead author Robert Montgomery, MD, PhD, the H. Leon Pachter, MD, Professor of Surgery at the NYU Grossman School of Medicine. “This sets the stage for more successful clinical trials in the near future.”
The findings also confirmed that a pig kidney can effectively serve as a replacement for a human kidney, says Montgomery, who is also the chair of the Department of Surgery and director of the NYU Langone Transplant Institute.
The second report in Nature outlines immune activity in greater detail, says Montgomery, who is a co-author. The research team conducted a multi-omics analysis, which integrates information about gene function, gene expression (activity level), and proteins, as well as other data, to gain a holistic understanding of complex mechanisms at work in the immune system.
Measuring about 5,100 expressed human and pig genes in the pig xenograft, the authors identified every type of immune cell in the tissue, tracked immune behavior over the two-month period, and observed the organ rejection in day-by-day snapshots.
The analysis revealed three major immune responses against the pig kidney: on postoperative day (POD) 21, driven by a part of the human recipient’s immune system that responds generally to intruders (innate) rather than to a specific target; on POD 33, driven by a specific population of human white blood cells that engulf invaders (macrophages); and on POD 45, driven mostly by the human T cell response. Montgomery says that by measuring levels of various blood biomarkers the researchers were able to spot these attacks up to five days before they were clinically visible in the tissue.
“Our multi-omics analysis uncovers various biomarkers that shows promise as an early-warning system for pig organ rejection,” said study co-lead author Eloi Schmauch, PhD, from the Keating Lab in the Department of Surgery at the NYU Grossman School of Medicine.
“The specific immune reactions revealed in our investigation provide clear pig and human targets for therapies to improve the success of xenotransplantation to address the dire shortage of available organs,” said study senior author Brendan Keating, PhD, a faculty member in the Department of Surgery at the NYU Grossman School of Medicine.
According to Keating, now that the researchers understand which antibodies and T cells are damaging the transplanted pig kidney, they next plan to investigate what molecules the immune response is targeting through the different layers of DNA, RNA, and protein datasets generated.
Keating says future studies in other human decedents and in live patients are needed to confirm the findings.
The gene edited pig organ was provided by Revivicor, a subsidiary of United Therapeutics. Funding for the studies was provided by National Institutes of Health grants U19AI191396, P30CA016087, R01AI144522, S10RR027050, and S10OD020056. Additional study funding was provided by Yosemite, United Therapeutics, Imam Abdulrahman bin Faisal University, the Vaisala Fund, the Aarne Koskelon Foundation, the Antti and Tyyne Soininen Foundation, and the Finnish Cultural Foundation.
Montgomery consults with and holds roles with ProCure On-Demand; the Spanish Society of Transplantation; the Transplant Society; LiveOnNY; the National Kidney Foundation; Sanofi-Aventis U.S.; and Lung Biotechnology, a subsidiary of United Therapeutics, and PBC BioMed. He has also previously served on the advisory boards for the biotechnology companies eGenesis and Recombinetics. The terms and conditions of all of these relationships are being managed in accordance with NYU Langone Health policies and procedures.
Megan Sykes, MD, at Columbia University in New York City, is the senior author on the clinical study.
Brian Piening, PhD; at Providence Health in Portland, Ore, is co-lead author of the multi-omics study.
Alexey Stukalov, PhD; Serafim Batzoglou, PhD; Shadi Ferdosi, PhD; and Asim Siddiqui, PhD; at Seer in San Francisco, were key study collaborators.
Other NYU Langone researchers involved in the clinical study are Jeffrey Stern, MD; Jacqueline Kim, MD; Karen Khalil, PharmD; Vasishta Tatapudi, MD; Edward Skolnik, MD; Ian Jaffe, MD, MS; Imad Aljabban, MD; Tal Eitan, MD; Shivani Bisen, BA; Elaina Weldon, MS; Kevin Breen, MD; Yasmeen Saad, MA; Constanza Bay Muntnich, MD; Simon Williams, PhD; Weimin Zhang, PhD; Larisa Kagermazova, BS; Chandra Goparaju, PhD; Rebecca Dieter, PharmD; Nikki Lawson, RN; Dorry Segev, MD, PhD; Nicole Ali, MD; David Goldfarb, MD; Victoria Costa, MD; Timothy Hilbert, MD, PhD; Sapna Mehta, MD; Ramin Herati, MD; Harvey Pass, MD; Ming Wu, MD; Jef Boeke, PhD; Massimo Mangiola, PhD; Philip Sommer, MD; and Adam Griesemer, MD. Additional investigators are Farshid Fathi, PhD; Nathan Suek, MD; Benjamin Vermette, BA; and Aprajita Mattoo, MD, at Columbia University in New York City; Valentin Goutaudier, MD, MSc; Erwan Morgand, PhD; Fariza Mezine, MSc; Alessia Giarraputo, PhD; Patrick Bruneval, MD; and Aurelie Sannier, MD, at University Paris Cite in France; Idris Boudhabhay, MD, MS; and Alexandre Loupy, MD, PhD, at Cordelier Research Center in Paris; Amy Dandro, MSc; Ana Laura Fazio-Kroll, PhD; Lars Burdorf, MD, PhD; and David Ayares, PhD, at Revivicor in Blacksburg, Virginia; and Marc Lorber, MD, at United Therapeutics in Silver Spring, Maryland.
Other NYU Langone researchers involved in the mutli-omics study are Simon Williams, PhD; Ian Jaffe, MD, MS; Karen Khalil, PharmD; Jacqueline Kim, MD; Vasishta Tatapudi, MD; Imad Aljabban, MD; Tal Eitan, MD; Mercy Williams, PhD; Francesca Zanoni, PhD; Jon Albay, MS; David Andrijevic, MD; Berk Maden, MD; Vincent Mauduit, Ing.; Susanna Vikman, MS; Diana Argibay, MA; Zasha Zayas, MS; Leah Wu, BS; Kiana Moi, MS; Weimin Zhang, PhD; Elaina Weldon, MSN; Hui Gao, MS; Larisa Kagermazova, PhD; Brendan Camellato, PhD; Chan Wang, PhD; Divya Gandla, BS; Riyana Bhatt, BS; Sarah Gao; Andrew Chang, PhD; Han Chen; Jennifer Motter, MHS; Deepak Saxena, PhD; Adriana Heguy, PhD; Anoma Nellore, MD; Edward Skolnik, MD; Massimo Mangiola, PhD; Qian Guo; Ramin Herati, MD, Jeffrey Stern, MD; Adam Griesemer, MD; and Jef Boeke, PhD. Other study authors include Darragh O’Brien, PhD, at the University of Oxford in England; Alexa Dowdell, MS, Fred Robinson, PhD, Alexandra Bartlett, PhD, and Lauren Hamilton, BA, at Providence Health in Portland, Oregon; Maedeh Mohebnasab, MD, at the University of Pittsburg in Pennsylvania; Robin Bombardi, PhD, Ryan Taft, PhD, and Ali Crawford, PhD, at Illumina in San Diego; Billy Lau, PhD, and Michael Snyder, PhD, at Stanford University in California; Loren Gragert, PhD, at Tulane University in New Orleans; Rudaynah Al-Ali, PhD, and Alawi Habara, PhD, at Imam Abdulrahman Bin Faisal University in Dammam, Saudi Arabia; Scott Thomas, PhD, at the NYU College of Dentistry in New York City; Robert Fairchild, PhD, at the Cleveland Clinic in Ohio; Alexandre Loupy, MD, PhD, at the Paris Transplant Group in France; Michael Holmes, MD, PhD, at the University of Bristol in England; Anita Chong, PhD, at the University of Chicago in Illinois; Minna Kaikkonen, PhD, and Suvi Linna-Kuosmanen, PhD, at the University of Eastern Finland in Kuopio; David Ayares, PhD, at Revivicor in Blacksburg, Virginia; Manolis Kellis, PhD, at the Massachusetts Institute of Technology in Cambridge; and Marc Lorber, MD, at United Therapeutics in Silver Spring, Maryland.
About NYU Langone Health
NYU Langone Health is a fully integrated health system that consistently achieves the best patient outcomes through a rigorous focus on quality that has resulted in some of the lowest mortality rates in the nation. Vizient, Inc. has ranked NYU Langone No. 1 out of 118 comprehensive academic medical centers across the nation for four years in a row, and U.S. News & World Report recently ranked four of its clinical specialties number one in the nation. NYU Langone offers a comprehensive range of medical services with one high standard of care across seven inpatient locations, its Perlmutter Cancer Center, and more than 320 outpatient locations in the New York area and Florida. The system also includes two tuition-free medical schools, in Manhattan and on Long Island, and a vast research enterprise.
Media Inquiries
Shira Polan
Phone: 212-404-4279
Shira.Polan@NYULangone.org
Journal
Nature
Subject of Research
People
Article Title
Multi-omics analysis of a pig-to-human decedent kidney xenotransplant
Article Publication Date
13-Nov-2025
COI Statement
Montgomery consults with and holds roles with ProCure On-Demand; the Spanish Society of Transplantation; the Transplant Society; LiveOnNY; the National Kidney Foundation; Sanofi-Aventis U.S.; and Lung Biotechnology, a subsidiary of United Therapeutics, and PBC BioMed. He has also previously served on the advisory boards for the biotechnology companies eGenesis and Recombinetics. The terms and conditions of all of these relationships are being managed in accordance with NYU Langone Health policies and procedures.
Juan Ponce Enrile, known as the architect of the Philippines' martial law dictatorship, has died aged 101 © BULLIT MARQUEZ / POOL/AFP
