Wednesday, December 20, 2023

 

Corona pandemic has reduced the melting of Himalayan glaciers


Clean air would make water supplies safer for billions of people


Peer-Reviewed Publication

LEIBNIZ INSTITUTE FOR TROPOSPHERIC RESEARCH (TROPOS)

Smog-1 

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SMOG OVER NORTHERN INDIA ON THE EDGE OF THE HIMALAYAN GLACIERS.

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CREDIT: NASA EARTH OBSERVATORY IMAGE BY JOSHUA STEVENS, USING VIIRS DATA FROM NASA EOSDIS/LANCE




Pune/Leipzig. Reducing air pollution to levels similar to those during the coronavirus pandemic could protect the glaciers in the Himalayas and prevent them from disappearing by the end of the century. This is the conclusion reached by an international research team analysing the situation during the COVID-19 lockdown in 2020. The cleaner air has ensured that less soot has been deposited on the glaciers, resulting in 0.5 to 1.5 mm less snow melting per day. The rapid retreat of glaciers and the loss of snow cover already pose a threat to the sustainable water supply of billions of people in Asia who live in the catchment areas of rivers such as the Indus, Ganges and Yangtze. If emissions of air pollutants such as soot could be reduced to at least the level of the lockdowns, snowmelt could be reduced by up to half. A switch to clean energy supplies and lower-emission modes of transport would therefore bring significant benefits for sustainable water supplies, agriculture and ecosystems in large parts of Asia, the researchers write in the journal Atmospheric Chemistry and Physics (ACP).

 

 

The mountains of the Hindu Kush Himalayas (HKH) and the highlands of Tibet in Central Asia form the largest snow-covered region outside the poles. The meltwater from these glaciers feeds rivers in India and China, which fuel agriculture, hydropower generation and the economies of these countries. The Himalayan snowmelt in spring provides around half of the annual fresh water for around 4 billion people in South Asia and East Asia. But resources are dwindling: Global warming has already led to a loss of around 40 per cent of the Himalayan glacier area compared to the Little Ice Age in the Middle Ages. With the exception of a few Karakoram glaciers, the snow mass there has also decreased significantly over the last 30 years. Model simulations for extreme scenarios show that the melting snow in the Himalayas could cause the glaciers there to disappear by the end of the 21st century. This is worrying news for the water supply of several billion people.

 

The fact that glaciers are becoming thinner and thinner is partly due to climate change with higher air temperatures and changes in precipitation - in other words, long-term causes that will take decades to combat. However, short-term factors such as the distribution and deposition of light-absorbing particles such as dust and soot (black carbon (BC)) also play a major role in glacier melting. Earlier studies have already shown that soot melts the snow on glaciers more than greenhouse gases in the atmosphere. The increasing energy demand of densely populated South Asia has greatly increased emissions of greenhouse gases and soot particles in recent decades, leading to increased darkening and melting of snow.

 

The economic slowdown caused by the lockdown measures during the coronavirus pandemic led to a drastic decline in passenger and freight transport, industrial emissions and energy consumption in this region in 2020. As a result, air pollution with greenhouse gases and especially soot also decreased significantly: satellite observations showed cleaner snow with almost a third less light-absorbing pollution during the lockdown in Asia between March and May 2020. This led to a decrease in snowmelt of 25 to 70 mm in 2020 - compared to the 20-year average for the months of March to May in the western Himalayas. The changes in snow absorption and surface albedo thus ensured that around 7 cubic kilometres of meltwater remained in the Indus catchment area.

 

The international team of researchers from India, Germany and the UK used global simulations to analyse in detail the impact of reduced air pollution over high mountains in Central Asia during the COVID-19 lockdowns between March and May 2020: They used the ECHAM6-HAMMOZ chemistry-climate model, updated with an improved soot-snow parameterisation, to compare corona time with typical air pollution conditions. The corona simulations were performed with a COVID-19 emission inventory where emissions were calculated based on Google and Apple mobility data. Various observational data was also included in the new study: Snow cover and atmospheric opacity were determined using MODIS spectral data from NASA. These data were supplemented by solar photometer measurements from two Aerosol Robotic Network (AERONET) stations in Lahore (Pakistan) and Dushanbe (Tajikistan). The AERONET measurements in Dushanbe were part of the joint German-Tajik CADEX project from 2014 to 2016, in which the Academy of Sciences of Tajikistan and TROPOS jointly analysed mineral dust over Central Asia.

 

The ECHAM6-HAMMOZ model simulations show that the COVID lockdown in spring 2020 led to a cleaner atmosphere over the mountains of the Hindu Kush Himalayas and the highlands of Tibet. "The aerosol optical thickness (AOD), i.e. the atmospheric opacity, over this region decreased by around 10 per cent in April 2020 compared to before the pandemic. This is supported by measurements from NASA's Moderate Resolution Imaging Spectroradiometer (MODIS), which also show a reduction in AOD compared to the average of the last 20 years," reports Dr Suvarna Fadnavis from the Indian Institute of Tropical Meteorology (IITM). The decrease in soot was also observed in the ground-based measurements of the Aerosol Radiative Forcing Over India Network (ARFINET): over the Indian Gangetic Plain (>50%), Northeast India (>30%), the Himalayan regions (16%-60%) and Tibet (70%).

 

The reduction in anthropogenic air pollution led to less soot being deposited on the snow in large parts of the high mountains of Central Asia. According to this study, there were around 25 to 350 micrograms less soot per kilogramme of snow in spring 2020, which corresponds to up to a third of the soot concentration in the snow there. However, according to the model, soot concentrations in the snow have also risen sporadically in some areas in the Hindu Kush, the eastern Himalayas and the Kunlun Mountains. The seemingly paradoxical differences are due to the fact that soot interacts with solar radiation not only on the surface, but above all in the atmosphere. This leads to complex adjustments in atmospheric circulation and thus to changes in the transport and deposition of air pollutants. "Our simulations show that the decrease in soot concentration in the snow and the general reduction in air pollution and associated radiative effects reduced the short-wave radiative forcing at the surface by up to 2 watts per square metre in March to May 2020, resulting in less atmospheric warming. This lower warming of the snowpack and the tropospheric column is the combined effect of less soot in the snow and the changes in atmospheric concentrations of sulphate and soot," explains Dr Bernd Heinold from TROPOS. "In the model, we were able to show that the decrease in air pollution reduced snowmelt in spring 2020 by 0.5 to 1.5 millimetres per day and thus reduced the runoff meltwater in the year by up to half." The reduction in man-made pollution during the COVID-19 lockdown has therefore benefited the high mountains of Central Asia in many ways: increased reflectivity of the snow surface, reduced snowmelt and increased snow cover, as well as an increase in stored water due to reduced surface water runoff.

 

"Our results make it clear that of the two processes causing the retreat of the Himalayan glaciers - global climate change and local air pollution - a reduction in air pollution in particular could be a short-term help," emphasises Prof. Ina Tegen from TROPOS. "Even if we were to stop CO2 emissions immediately, temperatures would not initially fall. However, our results confirm the importance of reducing short-lived climate drivers such as soot and their complementary role in CO2 mitigation. Reducing air pollution to similar levels as during the COVID-19 lockdowns in 2020 could protect the Himalayan glaciers, which are otherwise at risk of disappearing by the end of the 21st century." Since 2000, the glaciers in the Himalayas have lost almost half a metre of ice per year. If air pollution could be reduced to the level it was at during the coronavirus pandemic, for example, then snowmelt could be reduced by up to half. Clean air measures would therefore not only benefit the health of billions of people in Asia, but also the water supply, agriculture and ecosystems in large parts of Asia.

Thick clouds of smoke from straw fires over India.

CREDIT

European Union, Copernicus Sentinel-3 data - Processed by COPERNICUSEU

The impacts of reduced pollution on snow brightening in the Himalayas and reduced surface water runoff, as observed during the 2020 COVID-19 lockdown period.

CREDIT

Fadnavis, S., Heinold, B., Sabin, T. P., Kubin, A., Huang, K., Rap, A., and Müller, R.: Air pollution reductions caused by the COVID-19 lockdown open up a way to preserve the Himalayan glaciers, Atmos. Chem. Phys., 23, 10439-10449, https://doi.org/10.5194/acp-23-10439-2023 , 2023. Figure 6 (c)

Same and different: A new species of pit viper from Myanmar


Peer-Reviewed Publication

PENSOFT PUBLISHERS

A specimen of Trimeresurus ayeyarwadyensis 

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A SPECIMEN OF TRIMERESURUS AYEYARWADYENSIS FROM THE YANGON REGION, MYANMAR.

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CREDIT: WOLFGANG WÜSTER




Finding and describing new species can be a tricky endeavor. Scientists typically look for distinctive characters that can differentiate one species from another. However, variation is a continuum that is not always easy to quantify. At one extreme, multiple species can look alike even though they are different species—these are known as cryptic species. At the other extreme, a single species can be highly variable, creating an illusion of being different species. But what happens when you encounter both extremes simultaneously?

Herpetologist Dr Chan Kin Onn (previously at the Lee Kong Chian Natural History Museum, Singapore, now with the University of Kansas Biodiversity Institute and Natural History Museum, USA) led a study describing a new species of pit viper from Myanmar that is both similar and different from its sister species. The discovery is published in the open-access journal ZooKeys.

“Asian pit vipers of the genus Trimeresurus are notoriously difficult to tell apart, because they run the gamut of morphological variation. Some groups contain multiple species that look alike, while others may look very different but are actually the same species,” they say.

The redtail pit viper (Trimeresurus erythrurus) occurs along the northern coast of Myanmar and is invariably green with no markings on its body. A different species called the mangrove pit viper (Trimeresurus purpureomaculatus) occurs in southern Myanmar. This species typically has distinct dorsal blotches, and incredibly variable dorsal coloration including gray, yellow, brown, and black, but never green. Interestingly, in central Myanmar, sandwiched between the distribution of the redtail pit viper and the mangrove pit viper, a unique population exists that is green with varying degrees of blotchiness, which appears to be a blend between the redtail pit viper and the mangrove pit viper.

“This mysterious population in central Myanmar baffled us and we initially thought that it could be a hybrid population,” the researchers said. In a separate paper, Dr Chan used modern genomic techniques and determined that the population in central Myanmar was actually a distinct species and not a hybrid population.

But this was not the end of the story. The researchers discovered another surprise when they examined the snake’s morphological features: they found that the new species was also highly variable. Certain populations are dark green with distinct blotches, easily distinguishable from its closest relative, the redtail pit viper, which is bright green with no blotches. However, some populations of the new species are bright green with no blotches and look virtually identical to the redtail pit viper.

“This is an interesting phenomenon, where one species is simultaneously similar and different from its closest relative (the redtail pit viper). We think that at some point in the past, the new species may have exchanged genes with the redtail pit viper from the north and the mangrove pit viper from the south,” says Dr Chan.

The new species is called the Ayeyarwady pit viper (Trimeresurus ayeyarwadyensis) in reference to the Ayeyarwady River, which is the largest and one of the most important rivers in Myanmar. The river forms an expansive delta that is bounded by the Pathein River to the west and the Yangon River to the east. These rivers and their associated basins also mark the westernmost and easternmost distribution boundaries of the Ayeyarwady pit viper.


 

A specimen of Trimeresurus ayeyarwadyensis from the Yangon Region, Myanmar.

CREDIT

Wolfgang Wüster

 

Original source:

Chan KO, Anuar S, Sankar A, Law IT, Law IS, Shivaram R, Christian C, Mulcahy DG, Malhotra A (2023) A new species of pit-viper from the Ayeyarwady and Yangon regions in Myanmar (Viperidae, Trimeresurus). ZooKeys 1186: 221-234. https://doi.org/10.3897/zookeys.1186.110422

 

TTUHSC researcher studies the ability of brine shrimp to thrive in high salinity


Results show how special sodium pump molecules adapt to high-salt environments


Peer-Reviewed Publication

TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER

Artigas_Pablo-web-3.jpg 

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TTUHSC’S PABLO ARTIGAS, PH.D., LED A RESEARCH TEAM THAT EXAMINED THE ADVANTAGES A NKA Α SUBUNIT VARIANT PROVIDES TO THE ABILITY OF BRINE SHRIMP TO SURVIVE IN EXTREME SALINITY. THEIR STUDY WAS PUBLISHED IN DECEMBER BY PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES.

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CREDIT: TTUHSC




Brine shrimp of the genus Artemia are small crustaceans that can thrive in environments where sodium concentrations are as high as 25% (more than eight times typical ocean sea water). Also known by the household pet trademark ‘sea monkeys,’ these animals are abundant in inland salt lakes where brine-fly larvae are the only other animals known to exist.

The mechanisms which permit brine shrimp to tolerate some of the harshest environments are only partially understood. Previously known adaptive features include a tight protective layer (integument) to avoid water loss and the increased extrusion of sodium (Na+) and chloride (Cl-) ions through specialized salt glands in the neck of larvae or in the swimming appendages of adults. 

The salt gland lining is an ion-transport tissue in which the transport of most ions is powered by the sodium-potassium ATPase (NKA), an essential protein pump found in all animals and formed by an alpha (α) and a beta (β) subunit. Most known NKA variants transport three Na+ ions out of the cell in exchange for importing two potassium (K+) ions. The Na+ gradient built by the NKA is then used by other proteins in the cell membrane to transport other substances. 

One brine shrimp NKA α subunit variant drastically increases abundance as salinity rises. The upregulation is extreme at salinities where not even brine fly larvae (which lack a special α subunit variant) can survive. To better understand the advantages this variant provides to the ability of brine shrimp to survive in extreme salinity, a research team led by Pablo Artigas, Ph.D., from the Texas Tech University Health Sciences Center (TTUHSC) School of Medicine’s Department of Cell Physiology and Molecular Biophysics (CPMB) and Center for Membrane Protein Research, examined the changes induced by salinity and the structure and function of the upregulated NKA variant.

The research team included Artigas’ CPMB and Center for Membrane Protein Research laboratory members Dylan J. Meyer, Ph.D., Victoria C. Young, Ph.D., Kerri Spontarelli and Jessica Eastman; and collaborators Evan Strandquist and Craig Gatto, Ph.D., from Illinois State University; Huan Rui, Ph.D., and Benoit Roux, Ph.D., from the University of Chicago; Matthew A. Birk, Ph.D., from St. Francis University (Pennsylvania); and Hanayo Nakanishi, Ph.D., and Kazuhiro Abe, Ph.D., from Nagoya (Japan) University.

Their study (“A Na pump with reduced stoichiometry is upregulated by brine shrimp in extreme salinities”) was published in December by Proceedings of the National Academy of Sciences (PNAS). The research was funded by a grant from the National Science Foundation.

Prior to embarking on structure function studies, the researchers discovered that brine shrimp have three α variants (instead of the two previously known) and two β variants (instead of one). The α subunit contains most of the protein components necessary for NKA function, while β is necessary for the NKA to reach the plasma membrane, where the NKA is localized for proper function. The upregulated NKA subunit is called α2KK because it has two amino acid substitutions, where lysine (a positively charged residue indicated by the single-letter code K) replaces asparagine (a polar, neutral residue) in the region where the sodium and potassium ions bind during the transport process.

The research team was able to solve the structure of the α2KK, which revealed that the two NKA-alpha2KK lysines were situated in a manner that could allow them to alter the number of Na+ and K+ ions the pump transports per cycle. The team then showed that the double-lysine containing NKA behaves like α2KK, and then demonstrated that these lysine-containing NKA variants transport with a different stoichiometry: two Na+ for one K+ instead of the Na+ for two K+ in each cycle. Stoichiometry is the calculation of the ratio between reactants and products during a chemical reaction or process.

This unique stoichiometry means that this special NKA variant uses more energy than canonical (commonly recognized) NKAs to transport Na+ and K+. Artigas said one way to envision why this is important is to think of the gradient as the height, and the sodium ions as the bricks that need to be lifted to that height. You may be able to lift three bricks at once from the floor, but may only be able to lift them a few inches. If you need to lift them six feet from the floor, one solution may be to lift a single brick three separate times. Another analogy could be the need to lower the gear in a car when going uphill, trading speed for power and using more gasoline to move.



“In other words, the Na+ gradient when the animals live at extreme salinity is so high, that the energy available in one ATP molecule is insufficient to move three Na+ ions, but enough to move two,” Artigas said. “Thus, our results show how the two lysines contribute to generate a pump with reduced stoichiometry, which allows these brine shrimp to maintain steeper Na+gradients in hypersaline environments. This unique adaptation allows brine shrimp to build and maintain the larger Naelectrochemical gradients imposed by their harsh environment.”

Michael Wiener, Ph.D., TTUHSC professor and chairperson for the CPMB and co-director of the Center for Membrane Protein Research, said the paper describes truly outstanding and fundamental science accomplished by the Artigas lab team and their national and international collaborators.

“Beyond the work itself — providing deep insight into how an essential-to-life molecular pump for sodium function in an organism that lives in a very high salt (sodium chloride) environment — there are further ramifications for the adaptation of organisms and the introduction of new organisms to environments such as lakes, ponds, streams and rivers that are becoming saltier with time due to climate change,” Wiener said.

When asked what’s next for the studies with brine shrimp, Artigas said the transport of salt across an epithelium is not the work of the NKA alone; other transport proteins participate in the process in coordination with NKA. With that in mind, the Artigas team is currently completing studies to identify these proteins and show that they are essential for the high-salinity adaptation process. 

“As exciting as the research questions to uncover essential biological principles are themselves, I am even more enthusiastic about the possibility of developing the use of brine shrimp for educational purposes,” Artigas said. “Brine shrimp are extremely easy to grow, and their adaptation mechanisms include other cool features such as the presence of a cyst dormant stage (cryptobiosis) able to tolerate even harsher conditions, such as desiccation and anoxia. We are currently working with Jessica D. Thomas, a biology teacher at Littlefield High School, to develop brine shrimp into an animal model for use in hands-on experiential scientific learning.”

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New larks revealed in Africa


Peer-Reviewed Publication

UPPSALA UNIVERSITY

The Plains Lark, Corypha kabalii 

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THE PLAINS LARK CORYPHA KABALII IS ONE OF THE LEAST-KNOWN LARK SPECIES IN THE WORLD. IT OCCURS MAINLY IN EASTERN ANGOLA AND NEIGHBORING PARTS OF THE DEMOCRATIC REPUBLIC OF THE CONGO AND ZAMBIA. IT IS GENETICALLY WELL SEPARATED FROM ITS CLOSEST RELATIVES AND ALSO HAS A UNIQUE FLIGHT DISPLAY. MINYANYA PLAIN, NORTH-WESTERN ZAMBIA, 2 NOVEMBER 2023.

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CREDIT: PER ALSTRÖM




Researchers at Uppsala University, together with colleagues at the Swedish Museum of Natural History, the University of Gothenburg, and institutions in seven other countries, have studied the relationships between five closely related species of larks that occur in Africa south of the Sahara. Two of these have not been observed for decades, so the researchers analysed DNA from museum specimens, some of which were over 100 years old.

The DNA analyses revealed nine distinct evolutionary lines, which were estimated to have been separated for up to at least five million years – which is about as long as humans and chimpanzees have been separated. Five of these branches on the tree of life are normally classified as different subspecies of Rufous-naped Lark Corypha africana and two of them as subspecies of Red-winged Lark Corypha hypermetra. Based on the genetic results, combined with extensive analyses of plumage, size/structure, vocalisations and behaviours, the researchers demonstrated that the relationships are considerably more complex and that the branches were previously missorted. They propose that the two species be split into seven. Conversely, the analyses showed that Ash’s Lark Corypha ashi, which is only known from a few specimens collected in Somalia, is the same species as the slightly better-known Somali Lark Corypha somalica.

Most of the ‘new’ species are extremely poorly known, and the paper describes songs and behaviours for the first time for several of them. After the article was accepted for publication, the lead author, Per Alström at Uppsala University, had the opportunity to study one of the least known ‘new’ species in north-western Zambia, on the border with Angola, in November 2023.

“This species, which we propose be called Plains Lark Corypha kabalii, but which does not yet have a Swedish name, is even more distinct than we concluded based on the data we analysed earlier,” Alström comments. “Among other things, it has a unique display behaviour, which is probably used both for defending a territory and attracting females. The Plains Lark male rapidly ascends to a height of a few metres, where it claps its wings to produce a rather strong sound before descending to the ground again on spread wings.”

Another recently published paper by largely the same research group presented the family tree for all but one of the more than 100 lark species of the world. That study confirmed that many species that are similar in appearance are not closely related at all, but likely developed similarities due to similar living conditions – so-called convergent evolution. Conversely, some close relatives have diverged so much in appearance that their close relationship can no longer be traced in their external characteristics.

 

Common insect species are suffering the biggest losses


Declines in insect numbers are largely driven by losses of more abundant species


Peer-Reviewed Publication

GERMAN CENTRE FOR INTEGRATIVE BIODIVERSITY RESEARCH (IDIV) HALLE-JENA-LEIPZIG

Monarch butterfly 

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MONARCH BUTTERFLIES (DANAUS PLEXIPPUS) ARE AN EXAMPLE OF A SPECIES WITH FORMERLY HIGH LOCAL ABUNDANCES THAT HAS DECLINED IN NUMBER

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CREDIT: T. HILL




Researchers at iDiv looked at long-term trends of land-based insects, such as beetles, moths, and grasshoppers, and found that decreases in the number of the formerly most common species have contributed most to local insect declines. Common or abundant insect species are those species that are locally found in the highest numbers, but which species these are differ among locations. The study’s findings challenge the idea that changes in insect biodiversity result from rarer species disappearing.

The study follows the recent sounding of alarm bells about insect loss, as researchers note dramatic declines in the total number of insects in many parts of the world. However, little is known about the general trends among locally rare and abundant species over long periods. “It was obvious this needed exploring,” says Roel van Klink, lead author of the study and senior scientist at iDiv and MLU. “We had to know whether observations about declines in total abundances of insects differed among common and rare species, and how this translated into changes in the overall insect diversity.”

More common species are losing out

Van Klink and colleagues set out to better understand trends in insect numbers by diving into past studies. They compiled a database on insect communities using data collected over periods between 9 and 64 years from 106 studies. For example, one Dutch study on ground beetles was started in 1959 and continues today. 

With this updated database, the researchers confirmed that despite variation among the data, on the whole, land-based insects from these long-term surveys are declining by 1.5% each year. To better understand this pattern, they compared the trends of species in different abundance categories and found that species that were the most abundant at the start of the time series showed the strongest average decline – around 8% annually – while rarer species declined less.

Importantly, the losses of previously dominant species were not compensated for by rises in other species, which has far-reaching implications: Abundant species are a staple food for birds and other insect-eating animals, making them essential for ecosystems. “Food webs must already be rewiring substantially in response to the decline of the most common species”, explains van Klink. “These species are super important for all kinds of other organisms and for the overall functioning of the ecosystem”.

Winners and Losers

The analysis clearly shows that the formerly abundant species are consistently losing the most individuals compared to the less abundant insect species. However, less abundant and rare species are also taking losses, driving declines in local species numbers. The study found a modest decrease in the overall number of species of just under 0.3% annually. This decline indicates that in addition to significant losses of common species, some rare species are going locally extinct. 

Coming out on top are new arrivals who managed to successfully establish themselves. Most of these new arrivals stay locally rare and replace other formerly rare insects, but occasionally they become very abundant. The invasive Asian Ladybeetle (Harmonia axyridis), which is now common throughout Europe, the Americas and South Africa, is one such example.

According to the paper’s authors, further research is necessary to determine the underlying causes of these trends. Although this study did not explicitly investigate possible causes, the declines are likely linked to recent human-related impacts, such as climate change and urbanisation, which are considered major drivers of biodiversity loss. “Insects seem to be taking a heavier hit than many other species as humans continue to dominate the planet,” explains Professor Jonathan Chase, senior author of the study and professor at iDiv and MLU. “Other studies, including those our team has worked on, have not found such diversity declines at local scales from many other groups of animals and plants”.

While the study’s results are striking, these trends are strongly biased to data on insect communities in Europe and North America. As such, they should not be interpreted as a global phenomenon. Chase adds: “The patterns we observed might be a best-case scenario for quantifying the real impact of people on insects,” referring to what scientists have called the lifeboat effect. “These declines were observed in long-term data from areas that have remained largely intact, sort of like a lifeboat, rather than in areas where massive conversion of natural areas into human-dominated landscapes has occurred, such as malls and parking lots”. 

Original publication
(Researchers with iDiv affiliation and alumni bold)

Roel van Klink, Diana E. Bowler, Konstantin B. Gongalsky, Minghua Shen, Scott R. Swengel, Jonathan M. Chase (2023). Disproportionate declines of formerly abundant species underlie insect loss. Nature, DOI: https://doi.org/10.1038/s41586-023-06861-4

 

Discovery: Plants use “trojan horse” to fight mold invasions 


Plant RNA defense systems hidden in unassuming “bubbles”


Peer-Reviewed Publication

UNIVERSITY OF CALIFORNIA - RIVERSIDE

Jin Lab 

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HAILING JIN, MICROBIOLOGY & PLANT PATHOLOGY DEPARTMENT PROFESSOR (2ND FROM LEFT), WITH STUDENTS STUDYING GRAY MOLD. 

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CREDIT: HAILING JIN/UCR




UC Riverside scientists have discovered a stealth molecular weapon that plants use to attack the cells of invading gray mold. 

If you’ve ever seen a fuzzy piece of fruit in your fridge, you’ve seen gray mold. It is an aggressive fungus that infects more than 1,400 different plant species: almost all fruits, vegetables, and many flowers. It is the second most damaging fungus for food crops in the world, causing billions in annual crop losses.

A new paper in the journal Cell Host & Microbe describes how plants send tiny, innocuous-seeming lipid “bubbles” filled with RNA across enemy lines, into the cells of the aggressive mold. Once inside, different types of RNA come out to suppress the infectious cells that sucked them in.

“Plants are not just sitting there doing nothing. They are trying to protect themselves from the mold, and now we have a better idea how they’re doing that,” said Hailing Jin, Microbiology & Plant Pathology Department professor at UCR and lead author of the new paper.

Previously, Jin’s team discovered that plants are using the bubbles, technically called extracellular vesicles, to send small RNA molecules able to silence genes that make the mold virulent. Now, the team has learned these bubbles can also contain messenger RNA, or mRNA, molecules that attack important cellular processes, including the functions of organelles in mold cells. 

“These mRNAs can encode some proteins that end up in the mitochondria of the mold cells. Those are the powerhouses of any cells because they generate energy,” Jin explained. “Once inside, they mess up the structure and function of the fungal mitochondria, which inhibits the growth and virulence of the fungus.”

It isn’t entirely clear why the fungus accepts the lipid bubbles. Jin theorizes they might just be hungry. “The fungus likely takes up the vesicles because they just want nutrients. They don’t know those RNAs are hidden in the vesicles,” she said. 

The strategy is an efficient one for the plants, because one mRNA molecule can have an outsized effect on the fungus. “The beauty of delivering mRNA, instead of other forms of molecular weapons, is that one RNA can be translated into many copies of proteins. This amplifies the effect of the mRNA weapon,” Jin said. 

Mold also uses these same lipid bubbles to deliver small, damaging RNAs into the plants they are infecting to suppress host immunity, an ability developed as part of a co-evolutionary arms race. Because RNAs are easily degraded, the bubbles provide excellent protection for transporting vulnerable cargo, for both plants and fungi.

“During infections, there are always a lot of communications and molecule exchanges where plants and fungi try to fight against each other,” Jin said. “Previously people looked at proteins being exchanged. Now, modern technology has enabled us to discover another important group of players in this battle.”

Going forward, the scientists are hoping to use this discovery to create innovative, eco-friendly fungicides. “RNA-based fungicides would not leave toxic residue in the environment and would not affect humans or animals. RNA is present in most food, and it is easily digested,” Jin said. 

“There is a never-ending battle to control pests and pathogens. If we can deliver mRNA that interferes with mold cellular functions, we may be able to help plants more effectively fight in this battle.”

Gray mold overtaking strawberries. 

CREDIT

Steven Koike/UCCE