Tuesday, October 10, 2023

 

Could a specialized diet alleviate long COVID?


Keck Medicine of USC launches clinical trial to investigate if a nutritional intervention designed to reduce inflammation could curb the condition that so far has no cure


Business Announcement

UNIVERSITY OF SOUTHERN CALIFORNIA - HEALTH SCIENCES

Adupa Rao, MD, is an investigator of the long COVID clinical trial and medical director of the Keck Medicine Covid Recovery Clinic. 

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ADUPA RAO, MD, IS AN INVESTIGATOR OF THE LONG COVID CLINICAL TRIAL AND MEDICAL DIRECTOR OF THE KECK MEDICINE COVID RECOVERY CLINIC. 

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CREDIT: RICARDO CARRASCO III



LOS ANGELES — Approximately 7% of Americans have had long COVID, a range of ongoing health problems experienced after infection and recovery from COVID-19. Symptoms can include fatigue, brain fog, headaches, chest pain, heart palpitations and more.  

To date, there is no proven treatment for the syndrome, and the mechanisms that cause it are not fully understood.  

Now, a new clinical trial from Keck Medicine of USC is investigating if a diet designed to lower inflammation may play a role in easing this often debilitating condition.  

The premise of the trial revolves around recent research indicating that long COVID may be caused by a hyper inflammatory response that becomes activated during COVID-19 as the body fights off the virus but, in some people, does not recede even after the infection has passed. High inflammation levels in the body can lead to organ damage and other health problems.  

“We are examining if food choice can quiet the body’s inflammatory response and in doing so, effectively minimize or curtail long COVID symptoms,” said Adupa Rao, MD, an investigator of the clinical trial and medical director of the Keck Medicine Covid Recovery Clinic.  

The study will examine the anti-inflammatory effect of a low-carbohydrate diet to lower blood glucose (sugar) levels in combination with a medical food that raises blood ketone levels. Ketones, including beta-hydroxybutyrate, the active ketone in this food, are chemicals the body produces to provide energy when the body is low on carbohydrates and sugars. A low-carb diet and ketones have both been associated with reduced inflammation in the body.  

Researchers plan to enroll 50 long COVID patients being treated by Keck Medicine’s Covid Recovery Clinic. Half the individuals will receive a 30-day dietary intervention and half will not. At the end of the month, researchers will determine how patients tolerated the regimen as well as compare inflammatory markers and long COVID symptoms between the two groups of patients. 

Nuria Pastor-Soler, MD, PhD, is the principal investigator of the long COVID clinical trial and an associate professor of medicine at the Keck School of Medicine of USC. 

CREDIT

Ricardo Carrasco III

If the nutritional intervention is tolerated well by patients and improves their health issues, researchers plan to expand the clinical trial to a larger population.  

"Research like ours is vital to expand our understanding of long COVID and ultimately help identify effective treatments to improve patients’ quality of life,” said principal investigator of the clinical trial, Nuria Pastor-Soler, MD, PhD, who is also an associate professor of medicine at the Keck School of Medicine of USC. “The results of this trial will hopefully move us closer to potential solutions.” 

Ken Hallows, MD, PhD, a professor of medicine at the Keck School of Medicine, is also a researcher in this study. The clinical trial is funded by the Amy P. Goldman Foundation. 

To learn more about the Keck Medicine Covid Recovery Clinic, please email covidrecovery@med.usc.edu or call 323-442-9209. 

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For more information about Keck Medicine of USC, please visit news.KeckMedicine.org.  

Space weather disrupts nocturnal bird migration, study finds


Peer-Reviewed Publication

UNIVERSITY OF MICHIGAN




Graphics

It's well-known that birds and other animals rely on Earth's magnetic field for long-distance navigation during seasonal migrations.

But how do periodic disruptions of the planet's magnetic field, caused by solar flares and other energetic outbursts, affect the reliability of those biological navigation systems?

University of Michigan researchers and their colleagues used massive, long-term datasets from networks of U.S. Doppler weather radar stations and ground-based magnetometers—devices that measure the intensity of local magnetic fields—to test for a possible link between geomagnetic disturbances and disruptions to nocturnal bird migration.

They found a 9%-17% reduction in the number of migrating birds, in both spring and fall, during severe space weather events. And the birds that chose to migrate during such events seemed to experience more difficulty navigating, especially under overcast conditions in autumn.

The new findings, scheduled for online publication the week of Oct. 9 in Proceedings of the National Academy of Sciences, provide correlational evidence for previously unknown relationships between nocturnal bird migration dynamics and geomagnetic disturbances, according to the researchers.

"Our findings highlight how animal decisions are dependent on environmental conditions—including those that we as humans cannot perceive, such as geomagnetic disturbances—and that these behaviors influence population-level patterns of animal movement," said study lead author Eric Gulson-Castillo, a doctoral student in the U-M Department of Ecology and Evolutionary Biology.

Earth's magnetic field is regularly impacted by solar outbursts that can trigger colorful auroras and that sometimes disrupt satellite communications, human navigation systems and power grids.

But little is known about how those disturbances affect animals that depend on Earth's magnetic field for migratory orientation and navigation. Previous experimental studies over several decades provide strong evidence that birds, sea turtles and other organisms key into small changes in magnetic inclination, intensity and declination when making orientation decisions and developing navigational maps.

One recent study examined millions of bird banding records and found that geomagnetic disturbances were associated with increased incidence of migratory bird "vagrancy," that is, birds becoming lost during migration.

But most previous studies were narrowly focused in geographic extent, duration and the number of species examined. The newly published study, in contrast, uses a 23-year dataset of bird migration across the U.S. Great Plains to provide new insights at population and landscape levels.

The researchers used images collected at 37 NEXRAD radar stations in the central flyway of the U.S. Great Plains, a major migratory corridor. The flyway spans more than 1,000 miles in the U.S., from Texas to North Dakota.

The research team selected this relatively flat region to minimize influences from mountainous topography or oceanic and Great Lakes coastlines. Their final datasets included 1.7 million radar scans from the fall and 1.4 million from the spring.

The community of nocturnally migrating birds in this region is primarily composed of a diverse set of perching birds (Passeriformes, 73% of species) such as thrushes and warblers; shorebirds (Charadriiformes, 12%) such as sandpipers and plovers; and waterfowl (Anseriformes, 9%) such as ducks, geese and swans.

The NEXRAD radar scans detect groups of hundreds to thousands of migrating birds. Migration intensity—meaning the number of birds in each cluster—can be estimated and direction of flight can be measured.

Concurrent geomagnetic measurements were accessed through superMAG, a worldwide collection of geomagnetic ground stations. Data were collected from magnetometer stations near weather radar sites.

The researchers matched data from each radar station with a customized, spatiotemporally explicit index of geomagnetic disturbance that represents the maximum hourly change from background magnetic conditions.

U-M space scientist Daniel Welling and former University of Texas at Arlington undergraduate Michelle Bui compiled the space weather data and designed the geomagnetic disturbance index. Welling and Bui are co-authors of the new study.

"The biggest challenge was trying to distill such a large dataset—years and years of ground magnetic field observations—into a geomagnetic disturbance index for each radar site," said Welling, assistant professor in the Department of Climate and Space Sciences and Engineering at the U-M College of Engineering. "There was a lot of heavy lifting in terms of assessing data quality and validating our final data product to ensure that it was appropriate for this study."

The data trove was fed into two complementary statistical models to measure the putative effects of magnetic disturbances on bird migration. The models controlled for the known effects of weather, temporal variables such as time of night and geographic variables such as longitude and latitude.

"We found broad support that migration intensity decreases under high geomagnetic disturbance," said study senior author Ben Winger, assistant professor in the U-M Department of Ecology and Evolutionary Biology and a curator of birds at the U-M Museum of Zoology. 

"Our results provide ecological context for decades of research on the mechanisms of animal magnetoreception by demonstrating community-wide impacts of space weather on migration dynamics."

The researchers also found that migrating birds appear to drift with the wind more frequently during geomagnetic disturbances in the fall, instead of expending great effort to battle crosswinds.

"Effort flying" against the wind was reduced by 25% under cloudy skies during strong solar storms in the fall, suggesting that a combination of obscured celestial cues and magnetic disruption may hinder navigation.

"Our results suggest that fewer birds migrate during strong geomagnetic disturbances and that migrating birds may experience more difficulty navigating, especially under overcast conditions in autumn," said Gulson-Castillo, who conducted the study as part of his doctoral dissertation. "As a result, they may spend less effort actively navigating in flight and consequently fly in greater alignment with the wind."

In addition to Gulson-Castillo, Winger, Welling and Bui, the authors of the PNAS paper are Benjamin Van Doren of Cornell University, Kyle Horton of Colorado State University, Jing Li of the U-M Department of Statistics and Department of Biostatistics, Mark Moldwin of U-M's Department of Climate and Space Sciences and Engineering, and Kerby Shedden of the U-M Department of Statistics.

Winger and Moldwin were supported by a University of Michigan MCubed grant. Gulson-Castillo was supported by a National Science Foundation Graduate Research Fellowship Grant and a U-M Rackham Merit Fellowship. Van Doren was supported by a Cornell Presidential Postdoctoral Fellowship. Moldwin was also supported by an NSF grant.

"Space weather disrupts nocturnal bird migration," DOI 10.1073/pnas.2306317120

 

Climate-driven extreme heat may make parts of Earth too hot for humans


Peer-Reviewed Publication

PENN STATE





If global temperatures increase by 1 degree Celsius (C) or more than current levels, each year billions of people will be exposed to heat and humidity so extreme they will be unable to naturally cool themselves, according to interdisciplinary research from the Penn State College of Health and Human Development, Purdue University College of Sciences and Purdue Institute for a Sustainable Future. 

Results from a new article published today (Oct. 9) in Proceedings of the National Academy of Sciences indicated that warming of the planet beyond 1.5 C above preindustrial levels will be increasingly devastating for human health across the planet.  

Humans can only withstand certain combinations of heat and humidity before their bodies begin to experience heat-related health problems, such as heat stroke or heart attack. As climate change pushes temperatures higher around the world, billions of people could be pushed beyond these limits.  

Since the start of the industrial revolution, when humans began to burn fossil fuels in machines and factories, temperatures around the world have increased by about 1 C, or 1.8 degrees Fahrenheit (F). In 2015, 196 nations signed the Paris Agreement which aims to limit worldwide temperature increases to 1.5 C above pre-industrial levels.  

The researcher team modeled global temperature increases ranging between 1.5 C and 4 C — considered the worst-case scenario where warming would begin to accelerate — to identify areas of the planet where warming would lead to heat and humidity levels that exceed human limits. 

“To understand how complex, real-world problems like climate change will affect human health, you need expertise both about the planet and the human body,” said co-author W. Larry Kenney, professor of physiology and kinesiology, the Marie Underhill Noll Chair in Human Performance at Penn State and co-author of the new study. “I am not a climate scientist, and my collaborators are not physiologists. Collaboration is the only way to understand the complex ways that the environment will affect people’s lives and begin to develop solutions to the problems that we all must face together.” 

A threat to billions 

The ambient wet-bulb temperature limit for young, healthy people is about 31 C, which is equal to 87.8 F at 100% humidity, according to work published last year by Penn State researchers. However, in addition to temperature and humidity, the specific threshold for any individual at a specific moment also depends on their exertion level and other environmental factors, including wind speed and solar radiation. In human history, temperatures and humidity that exceed human limits have been recorded only a limited number of times — and only for a few hours at a time — in the Middle East and Southeast Asia, according to the researchers.  

Results of the study indicate that if global temperatures increase by 2 C above pre-industrial levels, the 2.2 billion residents of Pakistan and India’s Indus River Valley, the one billion people living in eastern China and the 800 million residents of sub-Saharan Africa will annually experience many hours of heat that surpass human tolerance. 

These regions would primarily experience high-humidity heatwaves. Heatwaves with higher humidity can be more dangerous because the air cannot absorb excess moisture, which limits sweat evaporates from human bodies and moisture from some infrastructure, like evaporative coolers. Troublingly, researchers said, these regions are also in lower-to-middle income nations, so many of the affected people may not have access to air conditioning or any effective way to mitigate the negative health effects of the heat. 

If warming of the planet continues to 3 C above pre-industrial levels, the researchers concluded, heat and humidity levels that surpass human tolerance would begin to affect the Eastern Seaboard and the middle of the United States — from Florida to New York and from Houston to Chicago. South America and Australia would also experience extreme heat at that level of warming.  

At current levels of heating, the researchers said, the United States will experience more heatwaves, but these heatwaves are not predicted to surpass human limits as often as in other regions of the world. Still, the researchers cautioned that these types of models often do not account for the worst, most unusual weather events.  

“Models like these are good at predicting trends, but they do not predict specific events like the 2021 heatwave in Oregon that killed more than 700 people or London reaching 40 C last summer,” said lead author Daniel Vecellio, a bioclimatologist who completed a postdoctoral fellowship at Penn State with Kenney. “And remember, heat levels then were all below the limits of human tolerance that we identified. So, even though the United States will escape some of the worst direct effects of this warming, we will see deadly and unbearable heat more often. And — if temperatures continue to rise — we will live in a world where crops are failing and millions or billions of people are trying to migrate because their native regions are uninhabitable.” 

Understanding human limits and future warming 

Over the last several years, Kenney and his collaborators have conducted 462 separate experiments to document the combined levels of heat, humidity and physical exertion that humans can tolerate before their bodies can no longer maintain a stable core temperature.  

“As people get warmer, they sweat, and more blood is pumped to their skin so that they can maintain their core temperatures by losing heat to the environment,” Kenney said. “At certain levels of heat and humidity, these adjustments are no longer sufficient, and body core temperature begins to rise. This is not an immediate threat, but it does require some form of relief. If people do not find a way to cool down within hours, it can lead to heat exhaustion, heat stroke and strain on the cardiovascular system that can lead to heart attacks in vulnerable people.” 

In 2022, Kenney, Vecellio and their collaborators demonstrated that the limits of heat and humidity people can withstand are lower than were previously theorized.  

“The data collected by Kenney’s team at Penn State provided much needed empirical evidence about the human body’s ability to tolerate heat. Those studies were the foundation of these new predictions about where climate change will create conditions that humans cannot tolerate for long,” said co-author Matthew Huber, professor of earth, atmospheric and planetary sciences at Purdue University. 

When this work was published, Huber, who had already begun work on mapping the impacts of climate change, contacted Vecellio about a potential collaboration. Huber had previously published widely cited work proposing a theoretical limit of humans’ heat and humidity limits. 

The researchers, along with Huber’s graduate student, Qinqin Kong, decided to explore how people would be affected in different regions of the world if the planet warmed by between 1.5 C and 4 C. The researchers said that 3 C is the best estimate of how much the planet will warm by 2100 if no action is taken. 

“Around the world, official strategies for adapting to the weather focus on temperature only,” Kong said. “But this research shows that humid heat is going to be a much bigger threat than dry heat. Governments and policymakers need to re-evaluate the effectiveness of heat-mitigation strategies to invest in programs that will address the greatest dangers people will face.” 

Staying safe in the heat 

Regardless of how much the planet warms, the researchers said that people should always be concerned about extreme heat and humidity — even when they remain below the identified human limits. In preliminary studies of older populations, Kenney found that older adults experience heat stress and the associated health consequences at lower heat and humidity levels than young people. 

“Heat is already the weather phenomenon that kills the most people in the United States,” Vecellio, now a postdoctoral researcher at George Mason University’s Virginia Climate Center, said. “People should care for themselves and their neighbors — especially the elderly and sick — when heatwaves hit.” 

The data used in this study examined the body’s core temperatures, but the researchers said that during heatwaves, people experience health problems from other causes as well. For example, Kenney said that most of the 739 people who died during Chicago’s 1995 heatwave were over 65 and experienced a combination of high body temperature and cardiovascular problems, leading to heart attacks and other cardiovascular causes of death. 

Looking to the future 

To stop temperatures from increasing, the researchers cite decades of research indicating that humans must reduce the emission of greenhouse gases, especially the carbon dioxide emitted by burning fossil fuels. If changes are not made, middle-income and low-income countries will suffer the most, Vecellio said.  

As one example, the researchers pointed to Al Hudaydah, Yemen, a port city of more than 700,000 people on the Red Sea. Results of the study indicated that if the planet warms by 4 C, this city can expect more than 300 days when temperatures exceed the limits of human tolerance every year, making it almost uninhabitable.  

“The worst heat stress will occur in regions that are not wealthy and that are expected to experience rapid population growth in the coming decades,” Huber said. “This is true despite the fact that these nations generate far fewer greenhouse gas emissions than wealthy nations. As a result, billions of poor people will suffer, and many could die. But wealthy nations will suffer from this heat as well, and in this interconnected world, everyone can expect to be negatively affected in some way.” 

This research was supported by grants from the National Institute on Aging, the National Aeronautics and Space Administration, and the National Science Foundation. 

 Long-term lizard study challenges the rules of evolutionary biology

By lassoing lizards, putting tiny chips on their legs, and tracking them for three years, Georgia Tech's James Stroud revealed why species often appear unchanged for millions of years despite Charles Darwin's theory of constant evolution

Peer-Reviewed Publication

GEORGIA INSTITUTE OF TECHNOLOGY

Long-term lizard study challenges the rules of evolutionary biology 

IMAGE: 

A FEMALE BARK ANOLE (ANOLIS DISTICHUS) LIZARD. 

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CREDIT: JON SUH




Charles Darwin said that evolution was constantly happening, causing animals to adapt for survival. But many of his contemporaries disagreed. If evolution is always causing things to change, they asked, then how is it that two fossils from the same species, found in the same location, can look identical despite being 50 million years apart in age?

Everything changed in the past 40 years, when an explosion of evolutionary studies proved that evolution can and does occur rapidly — even from one generation to the next. Evolutionary biologists were thrilled, but the findings reinforced the same paradox: If evolution can happen so fast, then why do most species on Earth continue to appear the same for many millions of years?

This is known as the paradox of stasis, and James Stroud, assistant professor in the School of Biological Sciences at the Georgia Institute of Technology, set out to investigate it. He conducted a long-term study in a community of lizards, measuring how evolution unfolds in the wild across multiple species. In doing so, he may have found the answer to one of evolution’s greatest challenges.

His research was published as the cover story in the Proceedings of the Natural Academy of Sciences.

“We call this a paradox because it doesn’t seem to make any sense,” Stroud said. “The most common explanation is that natural selection is working to stabilize a species’ appearance, with the assumption that an average form will help them survive the best. The problem is, when people do field studies, they almost never find that this kind of ‘stabilizing’ selection actually exists.”

Lassoing Lizards

Stroud set up a field study with four different species of Anolis lizards (anoles) on a small island at the Fairchild Tropical Botanic Gardens in Coral Gables, Florida. He measured natural selection in all four lizard species over five consecutive time periods by catching and monitoring the survival of every lizard on the island.

Stroud and his colleagues searched day and night for lizards. Using long fishing poles with tiny lassos at their tips, they gently captured them by their strong necks, placed them in coolers, and documented the exact branch or stump where they found each lizard.

Back in the lab, Stroud measured the lizards’ heads, legs, feet, weight, and even the stickiness of their toes. After assigning an identifying number to each lizard and marking them with a tiny tag under the skin, the team released the lizards to the same branches where they’d found them. They went out in the following days and weeks to catch the rest of them.

Every six months for three years, Stroud and his team started the process over again. Catching the same lizards, taking measurements, releasing them, and making notes of which lizards survived and which didn’t.

A Picture of Evolution Is Worth a Thousand Lizards

By incorporating data for each time period, Stroud captured the history of every lizard in the community. He then related survival data to the variation in body traits, which allowed him to analyze which body traits were important predictors of survival. Taken together, the analysis painted a picture of how natural selection operated on the community as a whole.

To his surprise, Stroud found that the stabilizing form of natural selection — that which maintains a species’ same, average features — was extremely rare. In fact, natural selection varied massively through time. Some years, lizards with longer legs would survive better, and other years, lizards with shorter legs fared better. For other times, there was no clear pattern at all.

“The most fascinating result is that natural selection was extremely variable through time,” Stroud said. “We often saw that selection would completely flip in direction from one year to the next. When combined into a long-term pattern, however, all this variation effectively canceled itself out: Species remained remarkably similar across the entire time period.”

The findings provided by Stroud’s study had never been seen before. There had never been such insight into how selection works on a community level, and certainly not at this level of detail.

The reason scientists never understood how evolution works on the community level is because long-term studies like Stroud’s are extremely rare. Researchers are unlikely to undertake such projects because of the great amount of work and time required.

“Evolution can and does happen — it’s this ongoing process, but it doesn't necessarily mean things are constantly changing in the long run,” Stroud said. “Now we know that even if animals appear to be staying the same, evolution is still happening.”

According to Stroud, understanding evolution is critical to everything that we want to understand about life on Earth.

“Understanding evolution doesn’t only help us understand the plants and animals around us and how they're distributed across the world,” he said. “It also shows us how life sustains itself in a world dominated by humans.”

There have been very few studies that monitor how evolution unfolds in the wild at long time scales. That, according to Stroud, is why we have a biased view of what evolution is.

“For a very long time, evolutionary biologists have tried to figure out what was behind this paradox of stasis idea,” Stroud said. “What this study shows is that the answer may not be particularly complicated — we just had to conduct a study in the wild for a long enough time to figure it out.”

 

A green anole lizard (Anolis carolinensis). 

Taking high-resolution photographs of lizard feet to measure the size of adhesive sub-digital toepads.

Researchers identified the lizards by harmless blacklight tags that they implanted under the skin of their legs. 

James Stroud uses a tiny lasso attached to a fishing pole to catch a lizard. 

CREDIT

Day's Edge Prods

Citation: Stroud, J.T., et al. “Fluctuating selection maintains distinct species phenotypes in an ecological community in the wild.” PNAS, Oct. 2023.

DOI: https://doi.org/10.1073/pnas.2222071120

Writer: Catherine Barzler

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The Georgia Institute of Technology, or Georgia Tech, is one of the top public research universities in the U.S., developing leaders who advance technology and improve the human condition. The Institute offers business, computing, design, engineering, liberal arts, and sciences degrees. Its more than 45,000 undergraduate and graduate students, representing 50 states and more than 148 countries, study at the main campus in Atlanta, at campuses in France and China, and through distance and online learning. As a leading technological university, Georgia Tech is an engine of economic development for Georgia, the Southeast, and the nation, conducting more than $1 billion in research annually for government, industry, and society.

No lizard is an island


Peer-Reviewed Publication

WASHINGTON UNIVERSITY IN ST. LOUIS





Many species experience little to no change over long periods of time. Biologists often fall back on the same explanation for why this is true: that natural selection favors individuals with more moderate characteristics. Individuals with more extreme features — longer limbs, for example — have a disadvantage, while more moderate or average individuals are more likely to survive and reproduce, passing on their common features.

But new research from Washington University in St. Louis and the Georgia Institute of Technology provides a more complete explanation of how evolution plays out among species that live side-by-side. By directly measuring the long-term survival of lizards in the wild, the scientists showed that co-existing species each occupy a distinct “fitness peak” that is best understood as part of a communitywide “fitness surface” or landscape.

The study, led by James Stroud at Georgia Tech and publishing this week in the Proceedings of the National Academy of Sciences, offers a new way of thinking about how species relate to each other over time and how the differences between them reinforce their distinctness.

Jonathan Losos, the William H. Danforth Distinguished University Professor and a professor of biology in Arts & Sciences at Washington University, said: “If species are adapted to their environment, and the environment doesn’t change, then you wouldn’t expect the species to change. However, when scientists have gone out and studied natural selection, they rarely find evidence of such stabilizing selection.

“Given this disconnect, we set out to study natural selection on the organisms we know so well, Anolis lizards, to measure selection over several years and try to understand what’s going on,” Losos said.

Stroud, who was working as a postdoctoral researcher in Losos’ lab at WashU at the time, identified a place where four different species of anoles were living together on a small island in a lake in the Fairchild Tropical Botanical Garden near Miami.

He caught thousands of individual lizards on the island, tagged them and measured their body proportions. Stroud then re-caught all of the lizards on the island every six months for 2 ½ years, a period of time representing two to three generations of lizards.

New lizards that showed up were island babies, obviously. If a lizard disappeared from his census rolls, it was safe for Stroud to assume it had died, because the surrounding lake, filled with predatory fish, didn’t let them leave. By determining which lizards survived from one year to the next, the researchers could evaluate whether survival was related to the body traits they had been measuring, like leg length.

“What is special about this study is that we simultaneously measured natural selection on four co-existing species, something that has rarely been accomplished,” said Losos, who also serves as the director of the Living Earth Collaborative. “By coincidence, just as our paper was published, another group published a similar study on Darwin’s famous finches of the Galapagos Islands.”

In the Florida lizards, Losos and Stroud found that the stabilizing form of natural selection — that which maintains a species’ same, average features — was extremely rare. In fact, natural selection varied massively through time. Some years, lizards with longer legs would survive better, and other years, lizards with shorter legs fared better. For other times, there was no clear pattern at all.

“The most fascinating result is that natural selection was extremely variable through time,” Stroud said. “We often saw that selection would completely flip in direction from one year to the next. When combined into a long-term pattern, however, all this variation effectively canceled itself out: species remained remarkably similar across the entire time period.”

Scientists do not yet fully understand how evolution works on the community level. There are very few long-term studies like this one because of the great amount of work and time required.

“Evolution can and does happen — it’s this ongoing process, but it doesn’t necessarily mean things are constantly changing in the long run,” Stroud said. “Now we know that even if animals appear to be staying the same, evolution is still happening.”

 

Nature is inventive - the same substance is produced differently by plants


The production of special plant defense compounds has evolved independently in distantly related plant families


Peer-Reviewed Publication

MAX PLANCK INSTITUTE FOR CHEMICAL ECOLOGY

Aphelandra squarrosa and Lamium galeobdolon 

IMAGE: 

BENZOXAZINOIDS ARE PRODUCED IN VERY DIFFERENT PLANTS, SUCH AS THE ZEBRA PLANT APHELANDRA SQUARROSA (LEFT), THE GOLDEN DEAD-NETTLE LAMIUM GALEOBDOLON (RIGHT) AND MAIZE. COMPARATIVE STUDIES OF THE PLANT METABOLITES AND THE GENES EXPRESSED HAVE NOW DEMONSTRATED HOW FLEXIBLE PLANT METABOLISM IS. THE FORMATION OF BENZOXAZINOIDS IN THE THREE SPECIES IS BASED ON DIFFERENT ENZYME CLASSES AND HAS THUS EVOLVED INDEPENDENTLY OF ONE ANOTHER.

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CREDIT: KARIN GROTEN, MAX PLANCK INSTITUTE FOR CHEMICAL ECOLOGY




Maize plants form special compounds derived from indole, the so-called benzoxazinoids. They are considered ecologically important because they act against a wide range of herbivores and reduce their feeding. Benzoxazinoids also exhibit antimicrobial properties and are thought to be involved in mediating plant-plant interactions. Their biosynthesis in maize has been known since the 1990s. Meanwhile, their biosynthetic pathway has been described in several grasses, but benzoxazinoids have also been found in other plant species. Their distribution is peculiar: While specialized metabolites often occur in specific evolutionary related plant species, benzoxazinoids show the opposite behavior and occur sporadically in many distantly related plant families. Several attempts to elucidate this metabolic pathway not only in maize but also in distantly related species were unsuccessful. Accordingly, the research goal of Tobias Köllner's group in the Department of Natural Product Biosynthesis at the Max Planck Institute for Chemical Ecology was clear: "We wanted to find out whether the ability to form benzoxazinoids evolved independently in different species."

The team used two distantly related eudicot plant species that produce benzoxazinoids for the studies: the golden dead-nettle Lamium galebodolon, which is found in sparse forests and forest edges on nutrient-rich soils in Europe, and the zebra plant Aphelandra squarrosa, a popular houseplant. For both species, the researchers created data sets of the compounds and genes expressed in different tissues and compared them to closely related species that do not produce benzoxazinoids. "This approach allowed us to identify candidate genes that may be involved in the formation of these compounds. We further characterized the candidate genes by expressing them in tobacco to find out if they are really involved in the production of benzoxazinoids," says Matilde Florean, first author of the study, describing their methodological approach.

The researchers were able to show that the benzoxazinoid metabolic pathway evolved independently in maize and the two species under investigation. Tobias Köllner continues, "We found that, in contrast to maize where a number of closely related cytochrome P450 enzymes carry out specific steps of the metabolic pathway, different enzyme classes as well as unrelated enzyme families of cytochrome P450 were recruited.” In particular the discovery that the golden dead-nettle and the zebra plant use a dual-function flavin-containing monooxygenase, rather than two different cytochrome P450 enzymes as in grasses, was completely unexpected. Overall, the research team was surprised to find such a diversity of enzymes performing the same reactions.

"With this work, we have shown how flexible plant metabolism can be. We have shown that plants can independently invent very different strategies to make the same chemical compounds, and this has happened at least three times in the evolutionary history of benzoxazinoids" Sarah O'Connor, director of the Department of Natural Product Biosynthesis summarizes the research findings. In the future, the team hopes to elucidate the biosynthesis of these compounds in even more plant families.

Tobias Köllner and Matilde Florean with their research plants, the golden dead-nettle Lamium galebodolon and the zebra plant Aphelandra squarrosa, in the Institute's greenhouse. In their current study, they show how these two plant species form benzoxazinoids.

CREDIT

Angela Overmeyer, Max Planck Institute for Chemical Ecology