Viruses on plastic pollution may quietly accelerate the spread of antibiotic resistance
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Plastisphere viruses: hidden drivers of antibiotic resistance dissemination
view moreCredit: Xue-Peng Chen, Di Wu & Dong Zhu
Plastic pollution does more than litter landscapes and oceans. According to a new perspective article published in Biocontaminant, viruses living on plastic surfaces may play an underrecognized role in spreading antibiotic resistance, raising concerns for environmental and public health worldwide.
When plastics enter natural environments, they quickly become coated with microbial biofilms known as the plastisphere. These plastic associated communities are already known hotspots for antibiotic resistance genes. The new study highlights that viruses, the most abundant biological entities on Earth, could be key players in moving these resistance genes between microbes.
“Most research has focused on bacteria in the plastisphere, but viruses are everywhere in these communities and interact closely with their hosts,” said corresponding author Dong Zhu of the Chinese Academy of Sciences. “Our work suggests that plastisphere viruses may act as hidden drivers of antibiotic resistance dissemination.”
Viruses can transfer genetic material between bacteria through a process called horizontal gene transfer. In plastisphere biofilms, where microbes are densely packed, viruses may more easily shuttle resistance genes across species, including to potential pathogens. Some viruses also carry auxiliary metabolic genes that can boost bacterial survival under stressful conditions, such as exposure to antibiotics or pollutants, indirectly favoring resistant microbes.
The authors point out that viral behavior appears to differ between environments. In aquatic plastispheres, viruses are more likely to adopt life strategies that promote gene transfer, potentially increasing resistance risks. In soils, viruses may instead limit resistant bacteria by killing their hosts. These contrasting roles highlight the need to consider environmental context when assessing the risks of plastic pollution.
“This perspective emphasizes that antibiotic resistance linked to plastics cannot be fully understood without including viral ecology,” said lead author Xue Peng Chen. “Incorporating viruses into a One Health framework will help us better evaluate the long term consequences of plastic pollution.”
The authors call for future studies to directly measure gene exchange between viruses and bacteria on plastics and to refine methods for detecting virus encoded resistance genes. Such insights could inform environmental monitoring and plastic waste management strategies aimed at reducing antibiotic resistance risks.
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Journal reference: Chen XP, Wu D, Zhu D. 2025. Plastisphere viruses: hidden drivers of antibiotic resistance dissemination. Biocontaminant 1: e018
https://www.maxapress.com/article/doi/10.48130/biocontam-0025-0020
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About Biocontaminant:
Biocontaminant is a multidisciplinary platform dedicated to advancing fundamental and applied research on biological contaminants across diverse environments and systems. The journal serves as an innovative, efficient, and professional forum for global researchers to disseminate findings in this rapidly evolving field.
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Method of Research
News article
Subject of Research
Not applicable
Article Title
Plastisphere viruses: hidden drivers of antibiotic resistance dissemination
Article Publication Date
11-Dec-2025
Tracing hidden sources of nitrate pollution in rapidly changing rural urban landscapes
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Tracing the nitrate source and process in rural-urban ecotone: integrated multi-tracer approach
view moreCredit: Zihan Zhao, Jiayu Zhao, Sidi Chen, Letian Ning, Zucong Cai & Yanhua Wang
Nitrate pollution has become one of the most widespread water quality challenges in intensively farmed regions around the world, threatening drinking water safety, aquatic ecosystems, and downstream lakes. A new study published in Nitrogen Cycling reveals how human activities in rural urban transition zones are reshaping the nitrogen cycle, allowing nitrate to move through rivers and groundwater and ultimately reach large freshwater lakes.
The research focuses on the rural urban ecotone of the Yangtze River Delta in eastern China, an area where agriculture, aquaculture, and residential development coexist. Using an integrated multi tracer approach, the research team traced nitrate sources and transformation processes across surface water and groundwater systems, offering one of the most detailed pictures to date of how nitrogen pollution travels through complex landscapes.
“Nitrate pollution does not come from a single source, and it does not stay in one place,” said corresponding author Yanhua Wang. “Our goal was to understand not only where nitrate originates, but also how it moves, transforms, and accumulates as water flows from farmland and villages toward major lakes.”
The team combined water chemistry measurements, dual stable isotope analysis, Bayesian mixing models, and a county scale nitrogen cascade model. This approach allowed them to distinguish nitrate derived from manure, chemical fertilizers, aquaculture effluent, soil leaching, and atmospheric deposition. Field samples were collected across multiple seasons from rivers, canals, and groundwater wells in two contrasting river networks upstream of Taihu Lake, one of China’s largest freshwater lakes.
The results show that nitrate contamination was widespread in both surface water and groundwater, often exceeding regional water quality thresholds. In traditional agricultural areas, manure was identified as the dominant source of nitrate, contributing nearly 70 percent of surface water nitrate and about 60 percent of groundwater nitrate across seasons. In more industrialized and aquaculture intensive zones, aquaculture effluent emerged as the main nitrate source during dry and wet seasons, highlighting a pollution pathway that is often underestimated.
“Our findings show that manure and aquaculture are major drivers of nitrate pollution, yet they are frequently overlooked compared to chemical fertilizers,” said Wang. “This has important implications for how we design pollution control strategies.”
The study also revealed clear differences in nitrogen transformation processes. Nitrification dominated in agricultural surface waters, especially during dry periods, while denitrification was more pronounced in groundwater, where low oxygen conditions allow microbes to convert nitrate into other nitrogen forms. These underground processes can reduce nitrate concentrations locally, but they may also produce greenhouse gases such as nitrous oxide.
By integrating isotopic evidence with a nitrogen cascade model, the researchers linked observed water pollution to long term changes in land use and agricultural practices. Rapid expansion of aquaculture and commercial crop production has significantly increased nitrogen losses to aquatic systems in some counties, while reductions in cropland area and livestock restructuring have altered nitrogen pathways in others.
“Surface water and groundwater are not separate systems,” Wang explained. “They act together as major transport pathways that deliver nitrogen from land to lakes. Effective water protection must consider both.”
The study underscores the need for coordinated watershed management strategies that address manure application, pond aquaculture, and fragmented cropland management. According to the authors, reducing nitrate pollution in large lakes like Taihu will require integrated solutions that balance agricultural productivity with environmental protection, especially in rapidly urbanizing regions.
This multi tracer framework provides a powerful tool for policymakers and scientists seeking to untangle complex pollution sources and design targeted strategies to protect water resources in agricultural landscapes worldwide.
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Journal Reference: Zhao Z, Zhao J, Chen S, Ning L, Cai Z, et al. 2025. Tracing the nitrate source and process in rural-urban ecotone: integrated multi-tracer approach. Nitrogen Cycling 1: e011
https://www.maxapress.com/article/doi/10.48130/nc-0025-0011
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About Nitrogen Cycling:
Nitrogen Cycling is a multidisciplinary platform for communicating advances in fundamental and applied research on the nitrogen cycle. It is dedicated to serving as an innovative, efficient, and professional platform for researchers in the field of nitrogen cycling worldwide to deliver findings from this rapidly expanding field of science.
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Method of Research
Experimental study
Subject of Research
Not applicable
Article Title
Tracing the nitrate source and process in rural-urban ecotone: integrated multi-tracer approach
Article Publication Date
30-Dec-2025
