Long-term nitrogen addition shifts soil microbial community structure and potential carbon-nitrogen and PAH metabolic functions
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Schematic of the 15-year nitrogen-gradient field trial and the analytical workflow — soil physicochemical analysis, shotgun metagenomic sequencing, co-occurrence network analysis and residual PAH profiling — linking fertiliser regime with microbial community structure and functional potential.
view moreCredit: Yongtao Xu, Tianen Zhang, Kunlong Hui, Ying Yuan
Nitrogen fertiliser underpins global food production, but decades of application also reshape soil chemistry and the microbial communities that drive nutrient cycling. However, far less is known about how this chronic nitrogen input interacts with legacy organic pollutants — such as polycyclic aromatic hydrocarbons (PAHs) — that persist in arable soils long after their emissions have ceased.
Reporting in the journal Emerging Contaminants, researchers from the Chinese Research Academy of Environmental Sciences and the China University of Mining and Technology analysed surface soil (0–20 cm) from a 15-year field trial comparing four nitrogen application rates. They combined soil physicochemical analysis, shotgun metagenomics, exploratory co-occurrence network reconstruction and residual PAH profiling to ask how sustained nitrogen loading restructures bacterial and fungal communities and their functional potential.
“Experiments of this duration are rare, because most fertiliser trials record yields and nutrient budgets rather than microbial genomes and contaminant residues,” says corresponding author Kunlong Hui.
The treatments left a clear chemical signature: relative to unfertilised controls, total nitrogen and calcium concentrations in the highest-nitrogen plots were 2.25 and 1.38 times higher. “Microbial networks responded differently at different rates,” shares Hui. “Under high nitrogen, average degree — how densely taxa are connected — rose by 15% for bacteria and 31% for fungi; under low nitrogen, network modularity fell by 12% and 30% respectively, pointing to looser, less compartmentalised community organisation.”
Metagenomic profiling associated high nitrogen with a greater relative representation of carbon-metabolism genes and with shifts in the abundance of genes assigned to the PAH-degradation pathway (ko00624). In particular, mantel tests showed that the link between community composition and residual PAH profiles depended on treatment and was strongest under low nitrogen, suggesting that fertiliser regime may influence how tightly the microbiome is coupled to contaminant fate.
“Our results show that long-term nitrogen fertilisation reshapes soil microbial communities and their functional potential for carbon, nitrogen and PAH metabolism,” says Hui. “These are association-based findings: they do not demonstrate pathway activity, PAH degradation rates or altered environmental risk. Measuring actual process rates and contaminant bioavailability is the necessary next step.”
Because the direction and strength of the microbial response depended on application rate, the authors suggested that no single prescription fits every field. “We recommend site-specific nitrogen management that weighs crop nutrient demand against the persistence of residual organic contaminants, rather than treating fertiliser decisions and legacy pollution as separate problems,” says Hui. “Pairing routine nutrient monitoring with metagenomic and residue-based endpoints would give land managers the evidence to do so.”
Contact the Author: Kunlong Hui (corresponding author), State Key Laboratory of Environmental Criteria and Risk Assessment, and Key Laboratory of Groundwater Pollution Simulation and Control of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Email: huikunlong@163.com
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Journal
Emerging Contaminants
Method of Research
Experimental study
Subject of Research
Not applicable
Article Title
Long-term nitrogen addition shifts soil microbial community structure and potential carbon-nitrogen and PAH metabolic functions
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