Monday, June 15, 2026

THE CASE FOR SYNTH BIO TO END ANIMAL TESTING

Rat kidneys grown in mice offer new insights into addressing organ donor shortages




International Society for Stem Cell Research

Rat Kidneys Grown in Mice Offer New Insights into Addressing Organ Donor Shortages 

image: 

Rat embryonic stem cells (red) generate kidney tissue in Osr1-deficient mouse embryos via interspecies blastocyst complementation. The nephron structure and collecting duct (E-cadherin, green) are predominantly derived from the rat donor cells. The DNA (blue) was used for counterstaining.

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Credit: Shunsuke Yuri and Ayako Isotani, Nara Institute of Science and Technology





Kidney transplantation remains the most effective treatment for end-stage kidney disease, yet a severe shortage of donor organs continues to limit access for millions of patients worldwide. With demand for kidney transplants expected to reach 5 million patients by 2030 and only a fraction of that need currently being met, researchers are exploring innovative approaches to generate transplantable organs.

In a study published today in Stem Cell Reports, Shunsuke Yuri of the National Center for Geriatrics and Gerontology, Japan and Ayako Isotani of the Nara Institute of Science and Technology, Japan, successfully generated rat-derived kidneys in mice using a technique known as interspecies blastocyst complementation. The researchers created mouse embryos genetically unable to form kidneys, leaving a developmental niche that could be filled by injected embryonic stem cells. When rat embryonic stem cells were introduced into these embryos, they contributed extensively to kidney formation, particularly to nephron progenitor cells and ureteric bud lineages, resulting in the generation of a rat cell-derived kidney.

Although the interspecies embryos did not survive to birth, preventing assessment of kidney function, the study demonstrates the potential of using one species to generate organs from another. The findings represent an important step toward future efforts to grow transplantable human organs in larger animals, such as pigs, with the long-term goal of helping address the global shortage of donor kidneys.

About Stem Cell Reports
Stem Cell Reports is the open access, peer-reviewed journal of the International Society for Stem Cell Research (ISSCR) for communicating basic discoveries in stem cell research, in addition to translational and clinical studies. Stem Cell Reports focuses on original research with conceptual or practical advances that are of broad interest to stem cell biologists and clinicians. Stem Cell Reports is a Cell Press partner journal. Find the journal on X: @StemCellReports.

About ISSCR
Across more than 80 countries, the International Society for Stem Cell Research (@ISSCR) is the preeminent global, cross-disciplinary, science-based organization dedicated to advancing stem cell research and its translation to medicine.

 

People show a natural tendency to walk to the left




Universidad Carlos III de Madrid






An international study led by the University de Navarra, with the participation of the Universidad Carlos III de Madrid (UC3M), has discovered that people exhibit a slight but consistent tendency to move toward the left when walking. The research, carried out in collaboration with researchers from Waseda University and the University of Tokyo (Japan), as well as Shanghai University (China), and published in Nature Communications, shows that this individual inclination influences how crowds spontaneously organize themselves and could help improve models used to manage pedestrian flows in crowded spaces.

The results identify an intrinsic locomotor bias—that is, a predisposition in the direction of movement—which helps explain certain collective patterns observed in groups of pedestrians. The study challenges the idea that these behaviors depend exclusively on social norms or human interaction, offering a new perspective for understanding how certain collective phenomena arise. In the long term, this knowledge could contribute to designing safer, more comfortable, and more efficient public spaces.

Until now, scientists attributed the spontaneous organization of crowds primarily to: Interactions between people; collision avoidance maneuvers; social norms regarding movement (such as walking on the right or left depending on the country); physical characteristics of the environment (such as walls or spatial boundaries). However, this study shows that part of these behaviors could originate from individual predispositions present before any social interaction.

"For decades, we thought that these collective patterns arose solely from the interaction between pedestrians. In our work, we have verified that a significant part of them does not emerge only when people gather, but is instead inherent to the individual," notes Iñaki Echeverría, a researcher in Physics and Applied Mathematics at the University of Navarra and first author of the paper.

The researchers observed that when a group of people is asked to walk, whether in an enclosed or completely open space, there is a slight tendency to move in a counterclockwise direction. Although this preference is small at an individual level, its effect is amplified when hundreds or thousands of people participate, generating collective patterns observable on a large scale.

"Not all group members exhibit this preference, but a large majority do tend to move counterclockwise. This inclination ends up dictating the direction the group follows and gives rise to visible collective patterns," Echeverría explains.

The counterclockwise tendency appears beyond cultural norms and the environment

To test traditional explanations, the team conducted an extensive experimental campaign over several years in Spain and Japan. Comparing both countries allowed them to analyze whether the phenomenon depended on how pedestrians try to avoid colliding while walking, given that people in both countries typically move and dodge each other on opposite sides. The trials included groups of adults walking in controlled spaces, schoolchildren moving freely in an open yard, preschool children, and participants completing routes individually.

Another explanation that the team has ruled out is that there is some social norm making us prefer moving in a counterclockwise direction. UC3M professor Anxo Sánchez designed a questionnaire based on his experience researching social norms in contexts like climate change to study this possibility.

"The results were clear: there was no notable social norm, but furthermore, if data analysis suggests anything, it is that the norm would be to walk in a clockwise direction—in complete contradiction with the experiment—meaning it cannot explain the observations," notes Anxo Sánchez, from the Mathematics Department at UC3M.

One of the most significant results was verifying that this tendency persisted even when the factors traditionally considered responsible disappeared. It appeared in young children who had not yet internalized many social norms related to pedestrian circulation, in open spaces without walls or obstacles, and in countries with different traffic habits.

"We wanted to find out if the phenomenon depended on cultural norms, interaction with the environment, or pedestrian avoidance strategies. The results indicate that none of these factors, on its own, fully explains what we observed," points out Iker Zuriguel, Professor of Applied Physics at the University of Navarra and one of the main authors of the study.

Testing individuals walking alone further confirmed that the directional preference was present at an individual level, ruling out that it was a property that only emerges when people form part of a group. The researchers also analyzed factors associated with laterality, such as being right- or left-handed, dominant foot, or ocular dominance, without finding evidence that these factors explain the observed behavior.

Designing spaces adapted to how people move

In addition to providing new knowledge about human behavior, the work could have applications in fields such as urban mobility and the management of large crowds. The researchers managed to mathematically reproduce the patterns observed in large groups using only information obtained from individuals walking alone, which reinforces the idea that some collective dynamics can be understood from shared individual characteristics.

This knowledge can be useful in environments with a high concentration of people, such as airports, train stations, shopping centers, or sports venues, where models are increasingly used to optimize routes and reduce congestion.

"Better understanding the factors that influence the way we move allows for the development of more precise models on how people circulate in shared spaces. This information could be useful for designing more efficient infrastructure and creating environments that better adapt to how we move in our daily lives," Zuriguel adds.

The authors remind us that social interactions and the environment remain key elements for understanding crowd behavior.

"The results suggest that individual predispositions also play a relevant role in the emergence of collective movements. Furthermore, the study opens up new questions about the biological origin of these tendencies, as similar phenomena are observed in other animal species, from schools of fish to insect colonies," Echeverría concludes.

 

Organism absorbs little of the minerals from nuts, points a study that simulated human digestion


An experiment conducted at the Federal University of São Paulo showed that only a fraction of copper and magnesium present in Brazil nuts and cashews is rendered available by the human body.




Fundação de Amparo à Pesquisa do Estado de São Paulo






The presence of minerals in oilseeds, such as Brazil nuts (Bertholletia excelsa) and cashews (Anacardium occidentale), does not guarantee that the body will utilize them. A study conducted at the Diadema campus of the Federal University of São Paulo (UNIFESP) in Brazil and published in the journal Química Nova evaluated the bioavailability of these nutrients. Bioavailability is defined as the fraction of the mineral actually released during simulated gastrointestinal digestion and potentially available for absorption. 

“The line of research I’ve been developing focuses on evaluating the actual nutritional value of the minerals present in the diet. In this context, we study foods of great economic importance in Brazil. Our group not only determines the total quantity [concentration] of elements present in foods, but also uses assays that simulate gastric and intestinal digestion processes on those samples,” explains Angerson Nogueira do Nascimento, a chemist, associate professor at UNIFESP, and coordinator of the study. It was conducted in partnership with the group led by Professor Dário Santos Junior.  

With support from FAPESP, the researchers focused their investigation on two widely consumed oilseeds in Brazil. Their goal was to analyze the behavior of essential minerals during simulated human digestion while taking into account the action of gastric and intestinal juices, as well as the characteristics of the digestive tract.

They evaluated four minerals: copper, which is important for red blood cell formation, energy production, and immune system maintenance; magnesium, which is essential for bone health and muscle function; manganese, which has antioxidant properties and plays a role in bone and connective tissue formation; and zinc, which is essential for the immune system, wound healing, and protein synthesis.

After selecting the samples, the scientists determined the total concentration of these minerals in the nuts. They then assessed the bioaccessibility of the nutrients using an in vitro gastrointestinal digestion assay because the total amount of a nutrient present in food does not necessarily correspond to the amount absorbed by the body. 

The researchers highlighted the difference between bioaccessibility and bioavailability. Bioaccessibility refers to the fraction of a nutrient that is released from the food matrix in the gastrointestinal tract and is available to be absorbed. Bioavailability, on the other hand, corresponds to the portion actually absorbed and utilized by the body, taking into account processes such as distribution, metabolism, and excretion. Bioaccessibility can be assessed in a laboratory through in vitro assays, but bioavailability requires studies with animals or humans. These studies demand significant resources and require approval from a research ethics committee.

For the experiment, the researchers simulated the conditions of the human digestive system in a laboratory setting. This included controlling the temperature, agitation, pH level, and enzymatic composition. Then, they compared the results with the daily recommended intake of minerals.

The results showed that approximately 56% of the copper and 52% of the magnesium in cashews were available for absorption after the simulated digestion process. However, manganese and zinc were found in insufficient quantities for reliable measurement (below the detection limit of the instrumental technique). 

In Brazil nuts, approximately 50% of the copper and 28% of the magnesium were bioaccessible. Manganese and zinc remained below the detection limit after the test.

Therefore, although the analyzed nuts contained considerable amounts of copper, magnesium, manganese, and zinc, only a portion of these elements is available for absorption by the human body.

“The results demonstrated that assessing a food’s nutritional value shouldn’t be limited to total nutrient concentration. It’s also essential to investigate how these elements behave under conditions that simulate the digestive system to estimate their actual availability for absorption,” says Nascimento. 

According to the researcher, the analyzed nuts should not be considered exclusive sources of these nutrients, but they can contribute to a balanced and diverse diet in a complementary way.  

About São Paulo Research Foundation (FAPESP)
The São Paulo Research Foundation (FAPESP) is a public institution with the mission of supporting scientific research in all fields of knowledge by awarding scholarships, fellowships and grants to investigators linked with higher education and research institutions in the State of São Paulo, Brazil. FAPESP is aware that the very best research can only be done by working with the best researchers internationally. Therefore, it has established partnerships with funding agencies, higher education, private companies, and research organizations in other countries known for the quality of their research and has been encouraging scientists funded by its grants to further develop their international collaboration. You can learn more about FAPESP at www.fapesp.br/en and visit FAPESP news agency at www.agencia.fapesp.br/en to keep updated with the latest scientific breakthroughs FAPESP helps achieve through its many programs, awards and research centers. You may also subscribe to FAPESP news agency at http://agencia.fapesp.br/subscribe

 

Horse owners' personality and attachment style shape how they interact with and care for their horses




University of Turku






A new study shows that horse owners' psychological characteristics, including their attachment styles and personality traits, are systematically linked to how frequently they ride, train, and spend quality time with their horses, as well as the equipment they choose to use. The findings offer new insights into how human psychology shapes horse welfare and management.

Attachment theory, originally developed to describe emotional bonds between humans, has increasingly been applied to human–animal relationships. Attachment style describes how individuals relate to others emotionally: those with high avoidant attachment tend to distance themselves emotionally and rely less on others, while those with high anxious attachment tend to seek closeness and fear rejection or abandonment.

"We were interested in whether these psychological tendencies, which are well-studied in human relationships, would also manifest in how people interact with their horses — and indeed they do," says lead author of the study, Postdoctoral Researcher Océane Liehrmann from the Department of Biology at the University of Turku, Finland, and the Department of Biosystem and Technology at the Swedish University of Agricultural Sciences, Sweden.

The international research group conducted an online survey, gathering responses from 2,239 horse owners worldwide. The survey assessed their attachment styles using the Horse Attachment Questionnaire (HAQ) validated by the research group, and their personality traits using the Short Five Questionnaire.

Owners reported how often they rode, did groundwork training, and spent quality time with their horses, as well as what type of headgear — bit, bitless, or both — they used.

More emotionally distant owners interact less with their horses

The results showed a clear pattern: owners who spent more time with their horses, whether riding, training, or simply being with them, tended to be more emotionally connected to their horses. In contrast, owners who kept more emotional distance to their horse also tended to interact with them less frequently, spending less time riding and engaging in hands-on activities.

"This mirrors what has been found in companion animal research, where people with higher avoidant attachment tend to be less attentive and less engaged in caring for their pets. Our findings suggest the same dynamic extends to horse ownership," notes Liehrmann.

Interestingly, the type of interaction and relationship length also mattered. Owners who regularly spent informal, unstructured quality time with their horse, simply being present with them without a specific training goal, tended to show stronger emotional bonds. Owners with longer relationships with their horse showed progressively lower avoidant attachment, with those knowing their horse for over 10 years being about 15 percent less avoidant than those in relationships of under one year.

Owners’ personality also played a role in how they engaged with their horses, though differences between groups were subtle. Those who rode more frequently tended to be slightly more organised, outgoing, and emotionally stable. In contrast, owners who practised groundwork more often tended to score higher in openness to new experiences, which may reflect a greater curiosity and willingness to explore non-traditional, horse-centred training methods.

"These are not dramatic differences, but they are consistent and meaningful. Personality shapes the way people approach activities across many domains of life, and equestrian practice appears to be no exception," Liehrmann explains.

Equipment choice linked to emotional distancing

The study also found subtle links between headgear choice and owner psychology. Owners who used bitless equipment or alternated between bit and bitless tended to score slightly lower in emotional distancing than those who rode exclusively with a bit. Owners open to using both types of headgear also tended to score higher in openness to new experiences, suggesting a greater willingness to explore different approaches to riding.

The researchers note that these are modest tendencies observed across a large group of owners, and that equipment choice is shaped by many factors, including riding discipline, training background, and the individual horse's needs.

Rather than pointing fingers at any particular practice, the findings suggest that reflecting on our own psychological tendencies could be a valuable step toward more conscious, horse-centred decision-making.

Implications for horse welfare and training

The researchers emphasise that even modest psychological differences may carry practical importance when they consistently influence how horses are managed and cared for over time.

"Understanding the psychological characteristics of horse owners could help tailor training programmes, improve human-horse communication, and ultimately support better horse welfare. Designing tools that give equestrians greater awareness of their own psychological tendencies could be a meaningful step in that direction," concludes Liehrmann.
 

 

The universal tempo of animal communication



A team from UNIGE, NCCR Evolving Language, reconnect and the Institut Pasteur shows that the vast majority of animal species have been vocalising at a shared rhythm for hundreds of millions of years.



Institut Pasteur





From insects to great apes, by way of birds and fish, animals communicate through an extraordinary variety of sounds. Whilst the pitch or timbre of their vocalisations matters, rhythm may play a more fundamental role. Scientists from the University of Geneva (UNIGE), the NCCR Evolving Language, the reConnect Institute and the Institut Pasteur analysed more than 2,000 sound recordings produced by 98 animal species. All of them vocalise at a strikingly similar rate — roughly two to three acoustic events per second — regardless of their size, habitat, species or social complexity. This constraint is likely linked to the brain’s capacity to process auditory stimuli, and human language is no exception. The findings are published in PLOS Biology.

Many animal species communicate using acoustic signals — calls, songs or vocalisations — that vary in frequency and tone. “But what about rhythm? Is there a common tempo, or does it adapt to the characteristics of each species? This temporal dimension is what we wanted to examine,” says Anne-Lise Giraud, director of the reConnect Institute, researcher at the Pasteur Institute and adjunct professor in the Department of Basic Neurosciences at the UNIGE Faculty of Medicine and Director of the reConnect Institute at the Institut Pasteur, who led the research.

The scientists analysed sounds produced by 98 species — mammals, birds, amphibians, insects, reptiles and fish — by developing a method to calculate vocalisation rate in a uniform way and assess which parameters might influence it. “We found that 95% of species fall between 0.45 and 4.99 vocalisations per second, with a marked concentration around 2.8 Hz — a striking degree of homogeneity for animals that are otherwise so different,” says Théophane Piette, postdoctoral researcher in the Department of Basic Neurosciences at the UNIGE Faculty of Medicine and first author of the study. “Neither body weight, lung capacity, social complexity nor habitat proved to be determining factors. This suggests that this rhythm is shaped by a shared, ancient constraint common across species, rather than by recent adaptations.”

A neural explanation

To analyse a sound, the brain must simultaneously integrate its overall structure and its fine details. The researchers therefore propose that auditory systems evolved around two complementary timescales. Slow oscillations, particularly in the delta band (1–4 Hz), would provide a long integration window for tracking acoustic sequences and identifying the general structure of sounds — a rate that also corresponds to that observed across many animal vocalisations. Conversely, faster processes, likely in the low gamma bands, would enable fine-grained temporal discrimination and the analysis of detailed acoustic structure. These faster mechanisms would contribute in particular to the identification of individual speakers or sound sources.

Towards cross-species communication?

Humans are no exception to this universal pattern in the animal kingdom. Although our speech rate is slightly above the animal average — partly owing to the structuring of language into syllables, words and sentences — we spontaneously slow down in challenging communication situations: background noise, an elderly interlocutor, or a young child.

This shared rhythm may have another consequence: facilitating communication between species. If most animals transmit and receive at the same basic rate, they may be able to decode signals produced by other species. That's exactly what dogs do with their owners: they process human speech at this same slow rhythm, and humans in turn tend to slow their speech when addressing them. “This common tempo may not merely reflect how brains process sound; it could also constitute a kind of universal synchronisation that facilitates communication across species,” concludes Théophane Piette.

This study is part of the work of the NCCR Evolving Language, co-directed by the universities of Geneva, Zurich and Neuchâtel, which aims to understand the biological foundations of language, its evolutionary origins and the challenges posed by new technologies.

 

Source

Animal acoustic communication has a conserved optimal rhythm within the neural delta range, PLOS Biology, 9th June 2026

Theophane Piette 1*, Chundra Cathcart2*, Chiaria Barbieri2,3,4, Keesha Martin Ming3,Didier Grandjean5, Balthasar Bickel2, Eloïse Déaux1*, Anne-Lise Giraud1,6*

1 Department of Basic Neurosciences, Faculty of Medicine, University of Geneva, Geneva, Switzerland,
2 Institute for the Interdisciplinary Study of Language Evolution (ISLE), University of Zurich, Zurich,Switzerland,
3 Department of Evolutionary Biology and Environmental Studies, University of Zurich,Zurich, Switzerland,
4 Department of Life and Environmental Sciences, University of Cagliari, Cagliari,Italy,
5 Swiss Center for Affective Sciences, University of Geneva, Geneva, Switzerland,
6 UniversitéParis Cité, Institut Pasteur, AP-HP, Inserm, Fondation Pour l’Audition, Institut de l’Audition, IHU reConnect,Paris, France
* These authors contributed equally to this work.

https://doi.org/10.1371/journal.pbio.3003798