Warming temps alone fail to trigger increased CO2 levels from soil
North Carolina State University
image:
Study co-author Paul Frankson of the University of Georgia looks over the soil-warming controls.
view moreCredit: Photo courtesy of Debjani Sihi, NC State University.
A study examining the effects of higher temperatures on soil shows that warming alone does not increase levels of carbon dioxide emitted from the soil. Instead, higher temperatures combined with more added carbon – and more nutrients like nitrogen and phosphorus – led to higher carbon dioxide levels released from the soil.
The findings provide another piece of the puzzle reflecting the role nature plays in the delicate balancing act between carbon storage in soil and carbon dioxide emissions into the atmosphere.
Much of the carbon dioxide emissions from soil come from microbes, tiny organisms like bacteria, fungi, viruses and others, that live in soil and “breathe out” carbon dioxide – just like people.
“When things warm up, there is more plant photosynthesis, more ‘food’ for microbes to metabolize on, more activity for microbes,” said Debjani Sihi, an assistant professor with joint appointments in NC State’s Department of Plant and Microbial Biology and Department of Crop and Soil Sciences and corresponding author of a paper describing the research.
“The question here is whether warming was enough to cause more carbon dioxide release from soil. The findings show that if you don't have the carbon and nutrients in easily available forms that soil microbes need to grow and thrive, then heating alone will not increase the loss of carbon.”
Sihi added that adding heat and nutrients alone also did not increase carbon dioxide emissions from the studied soil, which came from a long-term field-warming experimental site in the southeastern United States. Soil carbon in an easily available form was required for carbon dioxide levels from soil to increase.
Until recently, warming studies have mostly been conducted in cold (e.g., Arctic, boreal or temperate) climates, Sihi said, as researchers attempt to understand the effects in places where a little bit of warming might lead to large changes.
This study, in contrast, examined unfertile soil from a subtropical climate – Athens, Georgia, home to one of the longest-running soil-warming facilities on the planet.
“This study occurs in former cotton fields converted to forest land, not in native forest land,” Sihi said. “Cotton is an exhaustive crop, so the soil doesn’t contain many nutrients or carbon; the soil is not fertile or healthy.”
The researchers gathered soil from the field site and brought it to a lab to undergo heating – up to 2.5 degrees Celsius. They also examined a number of complex pathways in the soil carbon cycle, the process by which carbon is either stored in or expelled from the soil.
Soil holds many different forms of organic matter, from plant material to living and dead microbes, all of which play a part in the carbon cycle. Microbes are constantly searching for food to survive and grow. The researchers tracked how much carbon is stored in these different pools.
“Microbes are breathing and they are getting their energy from carbon. And then they're also fulfilling their demand of nutrients from the same food that they're getting,” Sihi said. “Like humans who need a balanced diet – an energy source, proteins, fiber – you can think about a similar parallel with microbes. They use some of the carbon to build biomass. And they will invest some energy to build enzymes that they need to break down complex organic matter into carbon and nutrients in forms that are easy for them to ingest. The remainder will just be expelled, because that's part of their metabolism.
“Nature emits carbon, but it also absorbs carbon. If you know how much CO2 comes from the natural system, then you can identify targets for different other industries or economic sectors to reduce carbon emissions.”
Sihi said that ongoing collaborative work is also examining a range of ecosystems, including two field warming experiments from the tropics – Puerto Rico and Panama – to understand how warming influences soil carbon loss.
“It appears in this case that warming alone may not stimulate microbial activities because these microbes actually don’t have a lot of resources to thrive in,” Sihi said. “In other words, depleted microbial resources constrain warming effects.”
The paper appears in Biogeochemistry. Yaxi Du, a former graduate student of Sihi’s, is the first author. Jacqueline Mohan and Paul Frankson from the University of Georgia co-authored the paper and maintained the long-term field-warming experiment used in the study. Greta Franke and Zhilin Chen are undergraduate researchers who assisted in Sihi’s lab.
Funding for the research was provided by the U.S. Department of Energy’s Environmental System Science Program awards DE-SC0024410 and DE-SC0025314.
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Note to editors: The abstract of the paper follows.
“Decoding the hidden mechanisms of soil carbon cycling in response to climate change in a substrate-limited forested ecosystem”
Authors: Debjani Sihi, North Carolina State University; Yaxi Du, Greta Franke and Zhilin Chen, Emory University; Jacqueline Mohan and Paul Frankson, University of Georgia
Published: Sept. 12, 2025 in Biogeochemistry
DOI: https://doi.org/10.1007/s10533-025-01265-0
Abstract: Climate change is rapidly redefining the biogeochemical dynamics of our planet, particularly in relation to soil organic carbon (SOC) storage and loss. Also, most existing soil warming studies have focused on nutrient-rich soils in temperate and arctic/boreal regions, limiting predictions for the many nutrient-poor tropical/subtropical soils that store a substantial fraction of global soil C. To address this gap, we evaluated the influence of temperature and substrate (C and nutrient) availability on soil C cycling in a nutrient poor (substrate-limited) subtropical forest, where previous field research suggested mixed warming responses. We aimed to isolate confounding elements and elucidate the principal mechanisms underpinning SOC dynamics under diverse environmental scenarios: warming (ambient at 25° C, +1.5 °C at 26.5 °C, and +2.5 °C at 27.5° C), nutrient addition (nitrogen and phosphorus) and carbon addition treatments. Samples were collected from a low-latitude soil warming experiment with subtropical Typic Kanhapludults soil (Whitehall Forest, Athens, Georgia). Under laboratory conditions, we incubated soil samples for 22 days at the temperatures recorded during sample collection in the field. We looked at key elements of the soil C cycle, including particulate and mineral associated organic C, microbial biomass C, and microbial necromass C. We also examined important processes like soil microbial respiration and enzyme kinetics. Our systematic evaluations helped us distinguish between the direct and indirect effects of warming (i.e., inherent and apparent temperature sensitivity) on SOC formation and loss. Our laboratory incubations showed that warming alone did not produce a sustained increase in microbial respiration or microbial biomass, underscoring the dominant role of C limitation in regulating microbial metabolism. In contrast, adding labile C alone or in combination with nutrients (N+P+C) significantly boosted microbial metabolism, supporting a co-limitation framework in which nutrient amendments became impactful only after alleviating C scarcity. Enzymatic assays further indicated that substrate depletion, rather than enzyme denaturation, constrained any prolonged warming effect. These findings underscore the need for continued research into SOC dynamics and microbial adaptation in nutrient-poor ecosystems, which remain underrepresented in Earth system models.
Journal
Biogeochemistry
Method of Research
Experimental study
Subject of Research
Not applicable
Article Title
Decoding the hidden mechanisms of soil carbon cycling in response to climate change in a substrate-limited forested ecosystem
Article Publication Date
12-Sep-2025
Chapman University research reveals tropical rainforest soils may fuel climate change as the Earth warms – Accelerating global warming
Chapman University
Orange, Calif. — Sept. 16, 2025 — A new study led by the U.S. Forest Service, with Chapman University as a key senior collaborator, published in Nature Communications, suggests the Earth’s own tropical soils may contribute to climate change as global warming continues, releasing vast amounts of carbon dioxide (CO₂) as they warm and potentially accelerating a dangerous feedback loop.
Tropical forests have long been viewed as critical allies in the fight against climate change, natural systems that absorb excess carbon and cool the planet. But this new research shows that warming itself is causing these forests’ soils to release enormous amounts of CO₂, essentially flipping the script.
This matters to everyone. If rainforests begin acting as carbon sources instead of sinks, it could accelerate global warming far faster than previously predicted, affecting everything from sea-level rise and extreme weather to food security and public health. Understanding these feedback loops is essential if we are to prepare for, and hopefully prevent, the worst impacts of a rapidly changing climate.
The international research team, including Chapman Assistant Professor of Biological Sciences Dr. Christine Sierra O’Connell, found that soil respiration in a Puerto Rican rainforest increased by 42–204% in experimentally warmed plots, one of the largest CO₂ release rates ever recorded in a terrestrial ecosystem. The findings position belowground ecosystems as critical players in the global climate crisis.
“This research shows that as the planet warms, tropical soils may begin to amplify that warming,” said O’Connell. “If these patterns persist across time and regions, we may be drastically underestimating the extent to which tropical forests will lose carbon and accelerate climate change.”
The study simulated a future climate scenario by raising atmospheric temperatures 4 °C using infrared heaters, marking the first such experiment in a tropical rainforest. Conducted through the TRACE (Tropical Responses to Altered Climate Experiment) project, which includes undergraduate researchers from Chapman University working alongside faculty in the field, the work suggests that microbes, not plant roots, were responsible for the dramatic CO₂ increases. These findings are significant because soils store more carbon than the atmosphere and all terrestrial plants combined. Releasing that carbon could amplify warming globally.
“We are witnessing a troubling shift,” O’Connell added. “The very systems we rely on to stabilize the climate may now be pushing us in the opposite direction.”
Researchers from the USDA Forest Service, US Geological Survey, University of Vermont, Morton Arboretum, and Michigan Technological University also contributed to the study.
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About Chapman University
Founded in 1861, Chapman University is a nationally ranked private university in Orange, California, about 30 miles south of Los Angeles. Chapman serves nearly 10,000 undergraduate and graduate students, with a 12:1 student-to-faculty ratio. Students can choose from over 100 areas of study within 11 colleges for a personalized education. Chapman is categorized by the Carnegie Classification as an R2 “high research activity” institution. Students at Chapman learn directly from distinguished world-class faculty including Nobel Prize winners, MacArthur fellows, published authors and Academy Award winners. The campus has produced a Rhodes Scholar, been named a top producer of Fulbright Scholars, and hosts a chapter of Phi Beta Kappa, the nation’s oldest and most prestigious honor society. Chapman also includes the Harry and Diane Rinker Health Science Campus in Irvine. The university features the No. 4 film school and No. 66 business school in the U.S. Learn more about Chapman University: www.chapman.edu.
Media Contact:
Bob Hitchcock, Director of Strategic Communications | rhitchcock@chapman.edu | Mobile: 407-388-4657
Journal
Nature Communications
Method of Research
Experimental study
Subject of Research
Not applicable
Article Title
Warming induces unexpectedly high soil respiration in a wet tropical forest
Article Publication Date
16-Sep-2025
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